Abstract：
:Schistosomiasis remains a severe problem of public health in developing countries. Several reports show that praziquantel, the drug of choice for treating schistosomiasis, can select Schistosoma mansoni strains resistant to the drug. Thus, developing new drugs against this parasitosis is a highly desirable goal. Curcumin, a phenolic compound deriving from the plant Curcuma longa, has been shown to have anticancer, anti-inflammatory and antiparasitic effects. Recently, our group has demonstrated that curcumin causes the separation of S. mansoni adult worm pairs, eggs infertility, decreased oviposition and parasite viability, leading to death. In the present work, we have investigated the effects of curcumin on S. mansoni gene expression in adult worms through microarray analyses. Our results showed 2374 genes that were significantly and differentially expressed, of which 981 were up-regulated and 1393 were down-regulated. Among the differentially expressed genes there were components of important signaling pathways involved in embryogenesis and oogenesis such as Notch and TGF-β. Gene networks most significantly enriched with altered genes were identified, including a network related to Cellular Function and Maintenance, Molecular Transport, Organ Development, which is connected to the TGF-β signaling pathway and might be related to the effect of curcumin on pairing of adult worm pairs, egg production and viability of worms. qPCR validation experiments with 7 genes have confirmed the expression changes detected with arrays. Here we suggest that transcriptional repression observed in Notch and TGF-β pathways could explain the effects on oviposition and egg development described in the literature.
journal_name
Mol Biochem Parasitoljournal_title
Molecular and biochemical parasitologyauthors
Morais ER,Oliveira KC,Magalhães LG,Moreira EB,Verjovski-Almeida S,Rodrigues Vdoi
10.1016/j.molbiopara.2012.11.006subject
Has Abstractpub_date
2013-02-01 00:00:00pages
91-7issue
2eissn
0166-6851issn
1872-9428pii
S0166-6851(12)00281-2journal_volume
187pub_type
杂志文章abstract：:Transfection has facilitated a functional analysis of transcriptional processes in the human malarial parasite Plasmodium falciparum, providing the first fascinating glimpses into the mechanisms regulating parasite development and pathogenicity. Here we review our rapidly evolving knowledge of what constitutes a promo...
journal_title：Molecular and biochemical parasitology
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doi：10.1016/0166-6851(82)90040-8
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journal_title：Molecular and biochemical parasitology
pub_type： 杂志文章
doi：10.1016/j.molbiopara.2010.10.005
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journal_title：Molecular and biochemical parasitology
pub_type： 杂志文章
doi：10.1016/0166-6851(92)90070-z
更新日期：1992-04-01 00:00:00
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journal_title：Molecular and biochemical parasitology
pub_type： 杂志文章
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doi：10.1016/0166-6851(93)90048-3
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journal_title：Molecular and biochemical parasitology
pub_type： 杂志文章
doi：10.1016/j.molbiopara.2012.05.002
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doi：10.1016/0166-6851(89)90054-6
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journal_title：Molecular and biochemical parasitology
pub_type： 杂志文章,评审
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journal_title：Molecular and biochemical parasitology
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journal_title：Molecular and biochemical parasitology
pub_type： 杂志文章,评审
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更新日期：2004-03-01 00:00:00
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journal_title：Molecular and biochemical parasitology
pub_type： 杂志文章
doi：10.1016/0166-6851(90)90029-l
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journal_title：Molecular and biochemical parasitology
pub_type： 杂志文章
doi：10.1016/0166-6851(94)90147-3
更新日期：1994-08-01 00:00:00
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journal_title：Molecular and biochemical parasitology
pub_type： 杂志文章
doi：10.1016/j.molbiopara.2005.03.020
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journal_title：Molecular and biochemical parasitology
pub_type： 杂志文章
doi：10.1016/s0166-6851(01)00223-7
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journal_title：Molecular and biochemical parasitology
pub_type： 杂志文章
doi：10.1016/0166-6851(91)90070-m
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journal_title：Molecular and biochemical parasitology
pub_type： 杂志文章
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更新日期：1993-12-01 00:00:00
