Characterization of tubulin genes in Trypanosoma rangeli.

abstract：:The nucleotide sequence of the cloned Echinococcus multilocularis DNA probe pAL1 was determined in order to simplify and improve the sensitivity of a diagnostic assay through the application of the polymerase chain reaction (PCR). The insert-specific oligonucleotides BG1 and BG2 define a 2.6-kb fragment in the genomic...

journal_title：Molecular and biochemical parasitology

authors： Gottstein B,Mowatt MR

更新日期：1991-02-01 00:00:00

abstract：:Localization of thymidylate synthase protein in Trichinella spiralis and Caenorhabditis elegans development was followed with the use of confocal microscopy, revealing similar expression patterns in both nematode species. In T. spiralis premature muscle larvae and C. elegans dauer, L3 and L4 larvae, thymidylate syntha...

journal_title：Molecular and biochemical parasitology

authors： Gołos B,Dąbrowska M,Wałajtys-Rode E,Zieliński Z,Wińska P,Cieśla J,Jagielska E,Moczoń T,Rode W

更新日期：2012-05-01 00:00:00

abstract：:Giardia lamblia is an early diverging eukaryote which causes gastrointestinal disease throughout the world. Different subgroups of Giardia have been defined based on several biochemical and genetic criteria. We have developed a method for stably introducing DNA into the nuclei of the parasite using puromycin acetyltra...

journal_title：Molecular and biochemical parasitology

authors： Singer SM,Yee J,Nash TE

更新日期：1998-04-01 00:00:00

abstract：:The glycosome, a microbody organelle found only in kinetoplastid protozoa, compartmentalizes the first six enzymes of glycolysis. In order to better understand the regulation and targeting of glycolytic enzymes in trypanosomes, we have cloned and analyzed the three genes of the phosphoglycerate kinase (PGK) complex of...

journal_title：Molecular and biochemical parasitology

authors： Parker HL,Hill T,Alexander K,Murphy NB,Fish WR,Parsons M

更新日期：1995-02-01 00:00:00

abstract：:The Plasmodium MSP-1 is a promising malaria vaccine candidate. However, the highly polymorphic nature of the MSP-1 gene (msp1) presents a potential obstacle for effective vaccine development. To investigate the evolutionary history of msp1 polymorphism in P. vivax, we construct phylogenetic trees of msp1 from P. vivax...

journal_title：Molecular and biochemical parasitology

authors： Tanabe K,Escalante A,Sakihama N,Honda M,Arisue N,Horii T,Culleton R,Hayakawa T,Hashimoto T,Longacre S,Pathirana S,Handunnetti S,Kishino H

更新日期：2007-11-01 00:00:00

abstract：:Most of the signaling pathways are regulated by reversible phosphorylation-dephosphorylation which involves enzymes- kinases and phosphatases. Current knowledge about the protein phosphatases in parasites like Trypanosoma and Leishmania is very minimal despite their enormousity. In present study, full length ORF of Le...

journal_title：Molecular and biochemical parasitology

authors： Jakkula P,Qureshi R,Iqbal A,Sagurthi SR,Qureshi IA

更新日期：2018-07-01 00:00:00

abstract：:Antibodies raised against a Leishmania major recombinant promastigote surface antigen 2 (PSA-2) fragment recognized three major polypeptides of approximate M(r) 96,000, 80,000 and 50,000 in promastigotes of three Israeli isolates of L. major including the cloned line LRC-L137-V121, but detected a different array of po...

journal_title：Molecular and biochemical parasitology

authors： Symons FM,Murray PJ,Ji H,Simpson RJ,Osborn AH,Cappai R,Handman E

更新日期：1994-09-01 00:00:00

abstract：:Investigating the proteome of intracellular Leishmania amastigotes has recently become possible due to the exploitation of fluorescence activated intracellular parasite sorting. Here, we employed this technology in combination with gel free analysis to greatly improve proteome coverage and suggest proteins putatively ...

journal_title：Molecular and biochemical parasitology

authors： Paape D,Barrios-Llerena ME,Le Bihan T,Mackay L,Aebischer T

更新日期：2010-02-01 00:00:00

abstract：:Among several protease inhibitors tested, only leupeptin was found to modify qualitatively the processing of P126, a major antigen of the parasitophorous vacuole of Plasmodium falciparum, and to inhibit the release of merozoites. Whereas P126 is normally processed upon merozoite release into 2 polypeptides of 50 and 7...

journal_title：Molecular and biochemical parasitology

authors： Debrabant A,Delplace P

更新日期：1989-03-01 00:00:00

abstract：:Phosphoserine phosphatase (PSP) catalyzes the third step of the phosphorylated serine biosynthetic pathway, and occurred multiple times in evolution, while enzymes catalyzing the first and second steps in the pathway have single respective origins. In the present study, we examined the existence of PSP among genus Ent...

journal_title：Molecular and biochemical parasitology

authors： Chiba Y,Makiuchi T,Jeelani G,Nozaki T

更新日期：2016-06-01 00:00:00

abstract：:We studied the substrate specificity requirements of recombinant cysteine peptidases from Plasmodium falciparum, falcipain-2 (FP-2) and falcipain-3 (FP-3), using fluorescence resonance energy transfer (FRET) peptides as substrates. Systematic modifications were introduced in the lead sequence Abz-KLRSSKQ-EDDnp (Abz=or...

journal_title：Molecular and biochemical parasitology

authors： Cotrin SS,Gouvêa IE,Melo PM,Bagnaresi P,Assis DM,Araújo MS,Juliano MA,Gazarini ML,Rosenthal PJ,Juliano L,Carmona AK

更新日期：2013-02-01 00:00:00

abstract：:Clones of the rRNA genes of Ostertagia ostertagi were selected from a library prepared from the genomic DNA of adult worms of strain LA-2. A 13.4-kb insert in a clone, lambda OOR78, is comprised of one complete 7.5-kb rDNA unit and portions of two adjacent units. The rDNA unit is directly repeated in a head-to-tail fa...

journal_title：Molecular and biochemical parasitology

authors： Dame JB,Yowell CA,Courtney CH,Lindgren WG

更新日期：1991-04-01 00:00:00

abstract：:Adult Schistosoma mansoni worms rapidly degrade their endogenous glycogen stores immediately after isolation from the host. In NCTC 109 or in a diphasic culture medium the glycogen levels slowly recovered again after the initial decrease. The rapid degradation of glycogen could be prevented, even in a simple salt medi...

journal_title：Molecular and biochemical parasitology

authors： Tielens AG,van den Bergh SG

更新日期：1987-07-01 00:00:00

abstract：:Neospora caninum is an obligate intracellular Apicomplexa, a phylum where one of the current methods for functional studies relies on molecular genetic tools. For Toxoplasma gondii, the first method described, in 1993, was based on resistance against chloramphenicol. As in T. gondii, we developed a vector constituted ...

journal_title：Molecular and biochemical parasitology

authors： Pereira LM,Yatsuda AP

更新日期：2014-09-01 00:00:00

abstract：:The scarcity of molecular tools for genetic manipulation is a critical obstacle for functional genomics studies on Trypanosoma cruzi. The current study adapted an inducible site-specific recombination system based on Dimerizable CRE recombinase (DiCRE). Two vectors for stable transfection were created, a first one to ...

journal_title：Molecular and biochemical parasitology

authors： Kangussu-Marcolino MM,Cunha AP,Avila AR,Herman JP,DaRocha WD

更新日期：2014-12-01 00:00:00

abstract：:The Plasmodium falciparum P-glycoprotein homologue 1 (PGH1) is structurally similar to several members of the ATP-binding cassette (ABC) superfamily of membrane transporters. We have examined whether the nucleotide binding domains predicted from the deduced amino sequence are functional by photoaffinity labeling of pu...

journal_title：Molecular and biochemical parasitology

authors： Karcz SR,Galatis D,Cowman AF

更新日期：1993-04-01 00:00:00

abstract：:Repeated DNA sequences have been instrumental in the development of DNA probes for many different parasites. Isolation of such DNA probes has generally been accomplished by differential screening of genomic libraries with total genomic DNA preparations. In the current work, a rational design strategy is presented for ...

journal_title：Molecular and biochemical parasitology

authors： Zimmerman PA,Toe L,Unnasch TR

更新日期：1993-04-01 00:00:00

abstract：:In trypanosomatids, removal of hydrogen peroxide and other aryl and alkyl peroxides is achieved by the NADPH-dependent trypanothione peroxidase system, whose components are trypanothione reductase (TRYR), trypanothione, tryparedoxin (TRYX) and tryparedoxin peroxidase (TRYP). Here, we report the cloning of a multi-copy...

journal_title：Molecular and biochemical parasitology

authors： Tetaud E,Giroud C,Prescott AR,Parkin DW,Baltz D,Biteau N,Baltz T,Fairlamb AH

更新日期：2001-09-03 00:00:00

abstract：:MRE11 is a conserved multi-functional protein that is important for maintaining genomic integrity in yeast and mammalian cells. By database searching, we identified a full-length candidate MRE11 on Trypanosoma brucei chromosome II. We subsequently cloned and sequenced the corresponding gene from the Lister 427 strain....

journal_title：Molecular and biochemical parasitology

authors： Tan KS,Leal ST,Cross GA

更新日期：2002-11-01 00:00:00

abstract：:We have cloned and sequenced a homologue of the ring-infected erythrocyte surface antigen (RESA) gene from Plasmodium falciparum designated RESA-2. Two reading frames with high homology to exon 1 and exon 2 of RESA at both the nucleotide and amino acid levels were identified in the RESA-2 sequence. However, RESA-2 doe...

journal_title：Molecular and biochemical parasitology

authors： Cappai R,Kaslow DC,Peterson MG,Cowman AF,Anders RF,Kemp DJ

更新日期：1992-09-01 00:00:00

abstract：:Cyclic AMP-dependent protein kinase (protein kinase A, PKA) is a key element in many cell signaling pathways. An essential role of Plasmodium falciparum PKA (PfPKA) activity was reported in the intraerythrocytic growth of the malaria parasite. However, molecular characterization of PfPKA using purified recombinant pro...

journal_title：Molecular and biochemical parasitology

authors： Sudo A,Kato K,Kobayashi K,Tohya Y,Akashi H

更新日期：2008-08-01 00:00:00

abstract：:The glycosylphosphatidylinositol (GPI)-anchored variant surface glycoprotein (VSG) of Trypanosoma brucei is the most abundant GPI-anchored protein expressed on any cell, and is an essential virulence factor. To determine what structural features affect efficient expression of VSG, we made a series of mutations in two ...

journal_title：Molecular and biochemical parasitology

authors： Wang J,Böhme U,Cross GA

更新日期：2003-05-01 00:00:00

abstract：:Reversible acetylation of core histones plays an important role in transcriptional regulation, cell cycle progression and developmental events. The acetylation state of histones is controlled by a dynamic equilibrium between activities of histone acetylase and deacetylase enzymes. Histone deacetylase (HDAC) was recent...

journal_title：Molecular and biochemical parasitology

authors： Joshi MB,Lin DT,Chiang PH,Goldman ND,Fujioka H,Aikawa M,Syin C

更新日期：1999-03-15 00:00:00

abstract：:To investigate whether the lysosomes of Trypanosoma brucei are capable of uptake of macromolecules after internalization by the cell, we used Triton WR-1339, a non-digestible macromolecular compound, which is known to cause a marked decrease in the density of hepatic lysosomes due to massive intralysosomal storage. In...

journal_title：Molecular and biochemical parasitology

authors： Opperdoes FR,Van Roy J

更新日期：1982-09-01 00:00:00

abstract：:Phosphatidylcholine (PC) is the most abundant phospholipid in the membranes of the human parasite Leishmania. It is synthesized via two metabolic routes, the de novo pathway that starts with the uptake of choline, and the threefold methylation of phosphatidylethanolamine. Choline was shown to be dispensable for Leishm...

journal_title：Molecular and biochemical parasitology

authors： Bibis SS,Dahlstrom K,Zhu T,Zufferey R

更新日期：2014-09-01 00:00:00

abstract：:The sequence of two fragments derived from the variable region of the kinetoplast maxicircle of Trypanosoma brucei has been determined. One fragment (1334 nucleotides, situated immediately upstream of the 12S and 9S ribosomal RNA genes) consists of non-repetitious DNA, which does not hybridize to other maxicircle regi...

journal_title：Molecular and biochemical parasitology

authors： de Vries BF,Mulder E,Brakenhoff JP,Sloof P,Benne R

更新日期：1988-01-01 00:00:00

abstract：:The heat shock response was studied as a model for control of gene expression and protein synthesis in Giardia lamblia. Cultured trophozoites were metabolically labelled with [35S]methionine, and proteins were analysed by SDS-polyacrylamide gel electrophoresis and fluorography. A temperature shift from 37 degrees C to...

journal_title：Molecular and biochemical parasitology

authors： Lindley TA,Chakraborty PR,Edlind TD

更新日期：1988-03-01 00:00:00

abstract：:In the mosquito, Plasmodium sporozoites rupture from oocysts found on the midgut wall, circulate in the hemolymph and invade salivary glands where they wait to be injected into a vertebrate host during a bloodmeal. The mechanisms by which sporozoites specifically attach to and invade salivary glands are not known but ...

journal_title：Molecular and biochemical parasitology

authors： Sidjanski SP,Vanderberg JP,Sinnis P

更新日期：1997-12-01 00:00:00

abstract：:The complete sequences of the genomic small subunit ribosomal RNA gene from two Phytomonas isolates: one associated with palm pathologies (P. cocos FGuiana) and one found in lactiferous plants with no apparent pathology (P. Euphorbe Senegal), were analyzed. Partial sequences from a number of other Phytomonas isolates ...

journal_title：Molecular and biochemical parasitology

authors： Marché S,Roth C,Philippe H,Dollet M,Baltz T

更新日期：1995-04-01 00:00:00

abstract：:Topoisomerases are enzymes that participate in many cellular functions involving topological manipulation of DNA strands. There are two types of topoisomerases in the cell: (a) type I topoisomerases; and (b) type II topoisomerases (topo II). Previously we have cloned and sequenced the gene encoding Trypanosoma cruzi t...

journal_title：Molecular and biochemical parasitology

authors： Fragoso SP,Mattei D,Hines JC,Ray D,Goldenberg S

更新日期：1998-08-01 00:00:00