Antigenic variation and cytoadhesion in Babesia bovis and Plasmodium falciparum: different logics achieve the same goal.

abstract：:Adult Schistosoma mansoni worms rapidly degrade their endogenous glycogen stores immediately after isolation from the host. In NCTC 109 or in a diphasic culture medium the glycogen levels slowly recovered again after the initial decrease. The rapid degradation of glycogen could be prevented, even in a simple salt medi...

journal_title：Molecular and biochemical parasitology

authors： Tielens AG,van den Bergh SG

更新日期：1987-07-01 00:00:00

abstract：:Transcriptional control of the hgl5 gene of Entamoeba histolytica is mediated through an unusual core promoter composed of TATA, GAAC and Initiator elements. In the hgl5 promoter the GAAC element (AATGAACT) determines the site and rate of transcription initiation. Here we tested the role of the GAAC element in transcr...

journal_title：Molecular and biochemical parasitology

authors： Singh U,Gilchrist CA,Schaenman JM,Rogers JB,Hockensmith JW,Mann BJ,Petri WA

更新日期：2002-03-01 00:00:00

abstract：:Phosphoinositides comprise a group of essential phospholipids that control a variety of cellular functions. In the case of the human malaria parasite Plasmodium falciparum, phosphoinositides have been shown to trigger exflagellation and to affect haemoglobin endocytosis and maturation of the parasite's digestive vacuo...

journal_title：Molecular and biochemical parasitology

authors： Krüger T,Sanchez CP,Lanzer M

更新日期：2010-08-01 00:00:00

abstract：:Two tandemly linked genes are present in the Leishmania infantum genome that code for the acidic ribosomal PO protein. The genes are identical in the coding region, although a striking lack of nucleotide sequence conservation is observed when the boundaries of the coding regions between both genes are compared. The 3'...

journal_title：Molecular and biochemical parasitology

authors： Soto M,Requena JM,Alonso C

更新日期：1993-10-01 00:00:00

abstract：:We have demonstrated that bradyzoites return to the tissue-cyst stage by a developmental pathway that is indistinguishable from that initiated by sporozoites. Mature bradyzoites, like sporozoites from oocysts, were non-proliferative as they contained uniform 1N DNA contents, and replication occurred only in parasites ...

journal_title：Molecular and biochemical parasitology

authors： Radke JR,Guerini MN,Jerome M,White MW

更新日期：2003-10-01 00:00:00

abstract：:The binding of tritiated benzimidazoles (BZs)-albendazole, parbendazole, oxibendazole, mebendazole, oxfendazole and fenbendazole-to crude tubulin extracts from BZ-susceptible and -resistant Haemonchus contortus has been examined. For all BZs, the binding was substantially lower in the resistant isolate. The extent of ...

journal_title：Molecular and biochemical parasitology

authors： Lacey E,Prichard RK

更新日期：1986-05-01 00:00:00

abstract：:A neutralizing antiserum, C16, raised against sporozoites of Theileria parva parva was used to screen a lambda gt11 expression library of T. parva parva (Muguga) genomic DNA fragments. Proteins encoded by one phage clone, lambda TpS-17, were reactive with the C16 antiserum. Detailed characterisation of the DNA insert ...

journal_title：Molecular and biochemical parasitology

authors： Iams KP,Young JR,Nene V,Desai J,Webster P,ole-MoiYoi OK,Musoke AJ

更新日期：1990-02-01 00:00:00

abstract：:Cysteine protease gene fragments from three protozoan parasites Trypanosoma cruzi, Trypanosoma brucei, and Entamoeba histolytica were amplified by the polymerase chain reaction (PCR) from genomic DNA using degenerate oligonucleotide primers. The primers used for the amplification were designed based upon amino acid se...

journal_title：Molecular and biochemical parasitology

authors： Eakin AE,Bouvier J,Sakanari JA,Craik CS,McKerrow JH

更新日期：1990-02-01 00:00:00

abstract：:Malarial merozoites use an array of ligands, including members of the Reticulocyte Binding Like (RBL) super-family of invasion proteins, to identify and invade erythrocytes. RBL family members are large Type I membrane anchored proteins expressed at the invasive end of merozoites that share homology with the Reticuloc...

journal_title：Molecular and biochemical parasitology

authors： Rayner JC,Huber CS,Galinski MR,Barnwell JW

更新日期：2004-02-01 00:00:00

abstract：:Pathogens of the Leishmania and Trypanosoma genera compartmentalize glycolytic and other nutritional pathways in glycosomes, unique subcellular organelles related to the peroxisomes of mammals and yeasts. Most glycosomal proteins are targeted to the glycosomes by a COOH-terminal tripeptide signal similar to the peroxi...

journal_title：Molecular and biochemical parasitology

authors： Jardim A,Rager N,Liu W,Ullman B

更新日期：2002-09-01 00:00:00

abstract：:Nucleotide-sugar transporters (NSTs) transport activated sugars (e.g. UDP-GlcNAc) from the cytosol to the lumen of the endoplasmic reticulum or Golgi apparatus where they are used to make glycoproteins and glycolipids. UDP-Glc is an important component of the N-glycan-dependent quality control (QC) system for protein ...

journal_title：Molecular and biochemical parasitology

authors： Banerjee S,Cui J,Robbins PW,Samuelson J

更新日期：2008-05-01 00:00:00

abstract：:The merozoite surface protein-1 of the human malaria parasite Plasmodium falciparum undergoes an extracellular proteolytic cleavage (secondary processing) intrinsic to successful erythrocyte invasion. In the T9/96 clone of P. falciparum the protease responsible has been characterised as a membrane-associated, calcium-...

journal_title：Molecular and biochemical parasitology

authors： Blackman MJ,Chappel JA,Shai S,Holder AA

更新日期：1993-11-01 00:00:00

abstract：:One of the candidate schistosome antigens for the development of a circulating antigen detection diagnostic assay is the circulating cathodic antigen (CCA). Detection of CCA in urine provides a non-invasive assay with high sensitivity. Previously we reported that CCA is secreted in patients' urine as a small molecular...

journal_title：Molecular and biochemical parasitology

authors： Abdeen HH,Attallah AF,Mansour MM,Harrison RA

更新日期：1999-06-25 00:00:00

abstract：:Actin-related proteins (Arps) constitute a family of eukaryotic cytoskeletal proteins involved in such diverse events as cell motility, cytokinesis, vesicle transport, and chromatin remodelling. Previously, in a study of Plasmodium berghei gene expression in ookinetes and oocysts, we detected stage-specific increased ...

journal_title：Molecular and biochemical parasitology

authors： Siden-Kiamos I,Schüler H,Liakopoulos D,Louis C

更新日期：2010-10-01 00:00:00

abstract：:Chloroquine is a critically important antimalarial drug and a well known intercalator into DNA. We now show that chloroquine binds more avidly to poly(dG-dC).poly(dG-dC) than to other synthetic polynucleotides and that this binding inhibits both salt- and cobalt-induced transitions to Z-DNA. These data are consistent ...

journal_title：Molecular and biochemical parasitology

authors： Kwakye-Berko F,Meshnick S

更新日期：1990-03-01 00:00:00

abstract：:The merozoite of Plasmodium vivax possesses a high molecular mass surface protein called Pv-merozoite surface protein 1, PvMSP-1, which exhibits antigenic diversity among isolates. In this study, the extent of sequence variation in the polymorphic region and the flanking interspecies conserved blocks (ICBs) 5 and 6 of...

journal_title：Molecular and biochemical parasitology

authors： Putaporntip C,Jongwutiwes S,Tanabe K,Thaithong S

更新日期：1997-01-01 00:00:00

abstract：:Cryptosporidium parvum is a protozoan parasite of the intestinal epithelium that has caused numerous outbreaks of diarrheal illness in humans. During our studies of the host immune response to C. parvum infection, we noted that two of the immunodominant surface antigens of the sporozoite stage of the parasite readily ...

journal_title：Molecular and biochemical parasitology

authors： Priest JW,Xie LT,Arrowood MJ,Lammie PJ

更新日期：2001-03-01 00:00:00

abstract：:Beta 1,3-glucan recognition proteins (GRP) have specific affinity for beta 1,3-glucan, a component on the surface of fungi and bacteria. By interacting with beta 1,3-glucan, GRP initiates activation of prophenoloxidase, a key enzyme in the signaling pathway leading to melanotic encapsulation in invertebrates. In this ...

journal_title：Molecular and biochemical parasitology

authors： Wang X,Fuchs JF,Infanger LC,Rocheleau TA,Hillyer JF,Chen CC,Christensen BM

更新日期：2005-01-01 00:00:00

abstract：:The exosome, a complex of 3'-exoribonucleases and associated proteins, is involved in the degradation of eukaryotic mRNAs in the cytoplasm, and has RNA processing and quality control functions in the nucleus. In yeast, the nuclear exosome differs from the cytoplasmic one in that it contains an additional non-essential...

journal_title：Molecular and biochemical parasitology

authors： Haile S,Cristodero M,Clayton C,Estévez AM

更新日期：2007-01-01 00:00:00

abstract：:Electron paramagnetic resonance spectrometry (EPR) techniques revealed a complex set of redox components in intact respiring cells of Tritrichomonas foetus, an aerotolerant anaerobic protozoon which lacks mitochondria. One of the two main systems of terminal respiration of this organism is the hydrogenosomal system, w...

journal_title：Molecular and biochemical parasitology

authors： Ohnishi T,Lloyd D,Lindmark DG,Müller M

更新日期：1980-10-01 00:00:00

abstract：:A gene-family, named sep, encoding small exported proteins conserved across Plasmodium species has been identified. SEP proteins (13-16 kDa) contain a predicted signal peptide at the NH(2)-terminus, an internal hydrophobic region and a polymorphic, low-complexity region at the carboxy-terminus. One member of the Plasm...

journal_title：Molecular and biochemical parasitology

authors： Birago C,Albanesi V,Silvestrini F,Picci L,Pizzi E,Alano P,Pace T,Ponzi M

更新日期：2003-02-01 00:00:00

abstract：:The effect of 9 monosaccharides which constitute cell surface carbohydrates on the infection of bovine embryonic skin and muscle (BESM) cells by Trypanosoma cruzi trypomastigotes and Toxoplasma gondii tachyzoites was assayed. Most of the monosaccharides tested stimulated the infection of BESM cells by T. gondii; none ...

journal_title：Molecular and biochemical parasitology

authors： Crane MS,Dvorak JA

更新日期：1982-05-01 00:00:00

abstract：:New phosphoproteins appear on the host erythrocyte membrane during Plasmodium berghei and P. chabaudi infection. Distinct proteins having similar properties and all distinguished by isoelectric points of less than 4.0 are identified. Associated with the erythrocyte membranes of P. berghei infected erythrocytes are two...

journal_title：Molecular and biochemical parasitology

authors： Wiser MF,Leible MB,Plitt B

更新日期：1988-01-01 00:00:00

abstract：:Parasitic infections are a widespread health problem and research of novel anthelmintic compounds is of the utmost importance. In this study we performed a virtual screening campaign by coupling ligand-based pharmacophore, homology modeling and molecular docking. The virtual screening campaign was conducted using a jo...

journal_title：Molecular and biochemical parasitology

authors： Stevanovic S,Marjanović DS,Trailović SM,Zdravković N,Perdih A,Nikolic K

更新日期：2021-01-07 00:00:00

abstract：:Leishmania major aquaglyceroporin (AQP1) is an adventitious metalloid channel that allows the bidirectional movement of arsenite and antimonite. Here we demonstrate that AQP1 is subjected to proteasome-dependent degradation. Treatment of Leishmania promastigotes with the proteasome inhibitor MG132 resulted in increase...

journal_title：Molecular and biochemical parasitology

authors： Sharma M,Mandal G,Mandal S,Bhattacharjee H,Mukhopadhyay R

更新日期：2015-06-01 00:00:00

abstract：:Three species of blood fluke from the genus Cardicola are known to parasitize and cause disease in Bluefin Tunas--C. forsteri, C. orientalis, and C. opisthorchis. Although initially believed to be separated by geography and host specificity, recent identification of at least two Cardicola spp. concurrently present wit...

journal_title：Molecular and biochemical parasitology

authors： Polinski M,Hamilton DB,Nowak B,Bridle A

更新日期：2013-09-01 00:00:00

abstract：:We have purified and characterized the Ascaris suum gamma-glutamylcysteine synthetase, the rate-limiting step in the glutathione biosynthesis. The purified enzyme exhibited a specific activity of 18 U (mg protein)-1. Estimation of the molecular mass of the native enzyme by FPLC on Superdex S-200 revealed the presence ...

journal_title：Molecular and biochemical parasitology

authors： Hussein AS,Walter RD

更新日期：1995-06-01 00:00:00

abstract：:A polyprotein composed of multiple units arranged in direct tandem arrays has been identified in parasitic and free living nematodes. Analysis of previously cloned units from the Dirofilaria immitis polyprotein antigen (DiPA) indicated the units were nearly identical but here we demonstrate that they segregate into tw...

journal_title：Molecular and biochemical parasitology

authors： Poole CB,Hornstra LJ,Benner JS,Fink JR,McReynolds LA

更新日期：1996-11-12 00:00:00

abstract：:Studies measuring the glycolytic intermediate and adenine nucleotide concentrations in Trypanosoma brucei metabolising glucose either aerobically or under conditions where glycerol-3-phosphate oxidase is inactive have shown the following: 1. Inhibition with 0.5 mM salicylhydroxamic acid (SHAM) accurately simulates ana...

journal_title：Molecular and biochemical parasitology

authors： Hammond DJ,Bowman IB

更新日期：1980-12-01 00:00:00

abstract：:To further analyze the organization of the nuclear rDNA locus in Trypanosoma cruzi, genomic recombinant plasmid clones were constructed and isolated after hybridization with rRNA molecules as hybridization probes. Approximately 11 kilobase pairs from the cistron were cloned in three recombinant plasmids carrying adjac...

journal_title：Molecular and biochemical parasitology

authors： Hernández R,Díaz-de Léon F,Castañeda M

更新日期：1988-01-15 00:00:00