Identification by extrachromosomal amplification and overexpression of a zeta-crystallin/NADPH-oxidoreductase homologue constitutively expressed in Leishmania spp.


:A gene which overexpresses a 36-kDa protein (p36) in tunicamycin-resistant Leishmania was mapped by transfection and overexpression to the upstream region of the drug maker in the extrachromosomal amplicon. Complete sequencing of this region revealed a single open reading frame of about 1 kb. Authenticity of the cloned gene is verified by immunologic specificity of its recombinant products and sequence identity with a p36 peptide. The gene shares an overall sequence similarity of about 50% with members of the eukaryote alcohol dehydrogenase family at the amino acid level, including essentially all 13 evolutionarily conserved residues and a nucleotide-binding domain. The binding ligands for both structurally and catalytically important zinc atoms are absent, similar to the zeta-crystallin/NADPH:quinone oxidoreductase gene. Consistent with hydrophilicity of its primary sequence and the presence of a nucleotide binding site, p36 is a soluble molecule non-sedimentable at 105,000 x g and binds Blue Sepharose, elutable only with NADPH. The p36 gene is expressed constitutively in both stages of the wild-type and is conserved among all Leishmania species examined, suggestive of its functional significance different from evolutionarily related homologues.


Mol Biochem Parasitol


Liu X,Chang KP




Has Abstract


1994-08-01 00:00:00














  • An S antigen gene from Plasmodium falciparum contains a novel repetitive sequence.

    abstract::The complete sequence of the gene coding for the S antigen from the Wellcome (West African) strain of Plasmodium falciparum has been obtained. It contains a central repetitive region consisting of 65 copies of a partially degenerate 24 bp sequence, coding for a conserved 8 amino acid repeat (Gly Pro Asn Ser Asp Gly As...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Nicholls SC,Hillman Y,Lockyer MJ,Odink KG,Holder AA

    更新日期:1988-02-01 00:00:00

  • Control of gene expression in Plasmodium falciparum.

    abstract::Transfection has facilitated a functional analysis of transcriptional processes in the human malarial parasite Plasmodium falciparum, providing the first fascinating glimpses into the mechanisms regulating parasite development and pathogenicity. Here we review our rapidly evolving knowledge of what constitutes a promo...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Horrocks P,Dechering K,Lanzer M

    更新日期:1998-09-15 00:00:00

  • Mediation of Trypanosoma cruzi invasion by heparan sulfate receptors on host cells and penetrin counter-receptors on the trypanosomes.

    abstract::Trypanosoma cruzi attaches and invades a large variety of mammalian cells by receptor-mediated interactions, one of them involving the binding of parasite trans-sialidase to host sialyl receptors. Three proteoglycan-deficient mutants of Chinese hamster ovary (CHO) cells were used to probe the role of host heparin and ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Herrera EM,Ming M,Ortega-Barria E,Pereira ME

    更新日期:1994-05-01 00:00:00

  • Helminth immunoregulation: the role of parasite secreted proteins in modulating host immunity.

    abstract::Helminths are masterful immunoregulators. A characteristic feature of helminth infection is a Th2-dominated immune response, but stimulation of immunoregulatory cell populations, such as regulatory T cells and alternatively activated macrophages, is equally common. Typically, Th1/17 immunity is blocked and productive ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Hewitson JP,Grainger JR,Maizels RM

    更新日期:2009-09-01 00:00:00

  • Carboxy-terminal sequence divergence and processing of the polyprotein antigen from Dirofilaria immitis.

    abstract::A polyprotein composed of multiple units arranged in direct tandem arrays has been identified in parasitic and free living nematodes. Analysis of previously cloned units from the Dirofilaria immitis polyprotein antigen (DiPA) indicated the units were nearly identical but here we demonstrate that they segregate into tw...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Poole CB,Hornstra LJ,Benner JS,Fink JR,McReynolds LA

    更新日期:1996-11-12 00:00:00

  • Coordinate regulation of a family of promastigote-enriched mRNAs by the 3'UTR PRE element in Leishmania mexicana.

    abstract::Post-transcriptional regulation is a key feature controlling gene expression in the protozoan parasite Leishmania. The nine-nucleotide paraflagellar rod regulatory element (PRE) in the 3'UTR of Leishmania mexicana PFR2 is both necessary and sufficient for the observed 10-fold higher level of PFR2 mRNA in promastigotes...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Holzer TR,Mishra KK,LeBowitz JH,Forney JD

    更新日期:2008-01-01 00:00:00

  • The P-glycoprotein-related gene family in Leishmania.

    abstract::P-glycoprotein gene amplification has been described in several drug-resistant parasitic protozoa. The first P-glycoprotein related gene described in Leishmania was ltpgpA, a gene frequently amplified in arsenite resistant Leishmania. Hybridization experiments indicated that ltpgpA was part of a gene family. In additi...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Légaré D,Hettema E,Ouellette M

    更新日期:1994-11-01 00:00:00

  • Regions in the 3' untranslated region confer stage-specific expression to the Leishmania mexicana a600-4 gene.

    abstract::Protozoan parasites of the genus Leishmania have a digenetic lifecycle, alternating between the promastigote and amastigote stages. The extracellular promastigote resides within a sandfly vector, while the obligate intracellular amastigote stage replicates in the phagolysosome of mammalian host macrophages. Adaptation...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Murray A,Fu C,Habibi G,McMaster WR

    更新日期:2007-06-01 00:00:00

  • Molecular epidemiology of giardiasis.

    abstract::Giardia duodenalis is a widespread parasite of mammalian species, including humans. Due to its invariant morphology, investigation on aspects such as host specificity and transmission patterns requires a direct genetic characterization of cysts/trophozoites from host samples. A number of molecular assays have been dev...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Cacciò SM,Ryan U

    更新日期:2008-08-01 00:00:00

  • Substrate specificity studies of the cysteine peptidases falcipain-2 and falcipain-3 from Plasmodium falciparum and demonstration of their kininogenase activity.

    abstract::We studied the substrate specificity requirements of recombinant cysteine peptidases from Plasmodium falciparum, falcipain-2 (FP-2) and falcipain-3 (FP-3), using fluorescence resonance energy transfer (FRET) peptides as substrates. Systematic modifications were introduced in the lead sequence Abz-KLRSSKQ-EDDnp (Abz=or...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Cotrin SS,Gouvêa IE,Melo PM,Bagnaresi P,Assis DM,Araújo MS,Juliano MA,Gazarini ML,Rosenthal PJ,Juliano L,Carmona AK

    更新日期:2013-02-01 00:00:00

  • Over-expression and localization of a host protein on the membrane of Cryptosporidium parvum infected epithelial cells.

    abstract::The genus Cryptosporidium includes several species of intestinal protozoan parasites which multiply in intestinal epithelial cells. The impact of this infection on the transcriptome of cultured host cells was investigated using DNA microarray hybridizations. The expression of 14 genes found to be consistently up- or d...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Yang YL,Serrano MG,Sheoran AS,Manque PA,Buck GA,Widmer G

    更新日期:2009-11-01 00:00:00

  • Theileria parva 104 kDa microneme--rhoptry protein is membrane-anchored by a non-cleaved amino-terminal signal sequence for entry into the endoplasmic reticulum.

    abstract::The 104 kDa microneme-rhoptry protein (p104) is the only known apical complex organelle-specific protein of Theileria parva. p104 exhibits striking structural similarities to circumsporozoite protein and sporozoite surface protein 2 of Plasmodium yoelii. Their primary sequences contain two hydrophobic segments, locate...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ebel T,Gerhards J,Binder BR,Lipp J

    更新日期:1999-05-15 00:00:00

  • Trypanosoma cruzi response to the oxidative stress generated by hydrogen peroxide.

    abstract::As an intracellular parasite, Trypanosoma cruzi is exposed to reactive oxygen species. The study of the proteins involved in the hydroperoxide detoxification cascade, tryparedoxin peroxidase included, may lead to the development of a more specific chemotherapy for Chagas'disease. In this work, the involvement of TcCPX...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Finzi JK,Chiavegatto CW,Corat KF,Lopez JA,Cabrera OG,Mielniczki-Pereira AA,Colli W,Alves MJ,Gadelha FR

    更新日期:2004-01-01 00:00:00

  • Molecular species analysis and quantification of the glycosylphosphatidylinositol intermediate glycolipid C from Trypanosoma brucei.

    abstract::The precursor of the glycosylphosphatidylinositol membrane anchor of the variant surface glycoprotein of Trypanosoma brucei is known as glycolipid A and it has the structure: EtN-PO4-6Man alpha 1-2Man alpha 1-6Man alpha 1-4GlcN alpha 1-6myo-inositol-PO4-(sn-1,2-dimyristoylglycerol). This precursor exists in equilibriu...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Güther ML,Treumann A,Ferguson MA

    更新日期:1996-05-01 00:00:00

  • Characterization of trafficking pathways and membrane genesis in malaria-infected erythrocytes.

    abstract::The origin of membraneous structures in the cytoplasm of human erythrocytes infected with the malaria parasite, Plasmodium falciparum, was determined by confocal fluorescence imaging microscopy. When infectious merozoites invaded erythrocytes labeled with the fluorescent, lipophilic, non-exchangeable molecules DiIC16 ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Pouvelle B,Gormley JA,Taraschi TF

    更新日期:1994-07-01 00:00:00

  • Molecular cloning, expression, and characterization of UDP N-acetyl-α-d-galactosamine: Polypeptide N-acetylgalactosaminyltransferase 4 from Cryptosporidium parvum.

    abstract::Cryptosporidium spp. are the causative agents of diarrheal disease worldwide, but effective treatments are lacking. Cryptosporidium employs mucin-like glycoproteins with O-glycans to attach to and infect host intestinal epithelial cells. The Tn antigen (GalNAcα1-Ser/Thr) is an O-glycan essential for these processes, a...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: DeCicco RePass MA,Bhat N,Heimburg-Molinaro J,Bunnell S,Cummings RD,Ward HD

    更新日期:2018-04-01 00:00:00

  • Schizodeme analysis of Trypanosoma cruzi stocks from South and Central America by analysis of PCR-amplified minicircle variable region sequences.

    abstract::Kinetoplast DNA (kDNA) was isolated from 56 stocks of Trypanosoma cruzi isolated from human patients, animals and insects from Brazil, Venezuela, Colombia and Costa Rica. Comparison of the patterns of digested kDNA on acrylamide gels led to the grouping of several stocks into two schizodemes. Schizodeme analysis was a...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Avila H,Goncalves AM,Nehme NS,Morel CM,Simpson L

    更新日期:1990-09-01 00:00:00

  • The proteasome-ubiquitin pathway in the Schistosoma mansoni egg has development- and morphology-specific characteristics.

    abstract::Schistosoma mansoni eggs, consisting of an ovum surrounded by nutritive vitelline cells packaged in a tanned protein shell, are produced by paired worms residing in the mesenteric veins of the human host. The vitelline cells are degraded as the larval miracidium matures, the fully developed egg either crossing the gut...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Mathieson W,Castro-Borges W,Wilson RA

    更新日期:2011-02-01 00:00:00

  • Cytochrome c and c1 heme lyases are essential in Plasmodium berghei.

    abstract::Malaria parasites possess a de novo heme synthetic pathway. Interestingly, this pathway is dispensable during the blood stages of development in mammalian hosts. The assembly of the two most important hemeproteins, cytochromes c and c1, is mediated by cytochrome heme lyase enzymes. Plasmodium spp. possess two cytochro...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Posayapisit N,Songsungthong W,Koonyosying P,Falade MO,Uthaipibull C,Yuthavong Y,Shaw PJ,Kamchonwongpaisan S

    更新日期:2016-01-01 00:00:00

  • Extensive homologies between Leishmania donovani chromosomes of markedly different size.

    abstract::The smallest chromosome (230 kb) of the HU3 stock of Leishmania donovani was purified from an orthogonal field alternation (OFAGE) gel, digested with PstI and cloned into the plasmid pUC13. When used to probe Southern blots of OFAGE gels, the cloned sequences recognised one or more large chromosomes in all L. donovani...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Bishop RP

    更新日期:1990-01-01 00:00:00

  • Molecular cloning and partial characterization of ribosomal RNA genes from Trypanosoma cruzi.

    abstract::To further analyze the organization of the nuclear rDNA locus in Trypanosoma cruzi, genomic recombinant plasmid clones were constructed and isolated after hybridization with rRNA molecules as hybridization probes. Approximately 11 kilobase pairs from the cistron were cloned in three recombinant plasmids carrying adjac...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hernández R,Díaz-de Léon F,Castañeda M

    更新日期:1988-01-15 00:00:00

  • The divergent N-terminal domain of Tim17 is critical for its assembly in the TIM complex in Trypanosoma brucei.

    abstract::Trypanosoma brucei Tim17(TbTim17), the single member of the Tim17/23/22 protein family, is an essential component of the translocase of the mitochondrial inner membrane (TIM). In spite of the conserved secondary structure, the primary sequence of TbTim17, particularly the N-terminal hydrophilic region, is significantl...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Weems E,Singha UK,Smith JT,Chaudhuri M

    更新日期:2017-12-01 00:00:00

  • Heat shock and stress response in Giardia lamblia.

    abstract::The heat shock response was studied as a model for control of gene expression and protein synthesis in Giardia lamblia. Cultured trophozoites were metabolically labelled with [35S]methionine, and proteins were analysed by SDS-polyacrylamide gel electrophoresis and fluorography. A temperature shift from 37 degrees C to...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Lindley TA,Chakraborty PR,Edlind TD

    更新日期:1988-03-01 00:00:00

  • Characterisation of the gene encoding a 104-kilodalton microneme-rhoptry protein of Theileria parva.

    abstract::A neutralizing antiserum, C16, raised against sporozoites of Theileria parva parva was used to screen a lambda gt11 expression library of T. parva parva (Muguga) genomic DNA fragments. Proteins encoded by one phage clone, lambda TpS-17, were reactive with the C16 antiserum. Detailed characterisation of the DNA insert ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Iams KP,Young JR,Nene V,Desai J,Webster P,ole-MoiYoi OK,Musoke AJ

    更新日期:1990-02-01 00:00:00

  • A role for tertiary structure in the generation of antigenic diversity and molecular association of the Tams1 polypeptide in Theileria annulata.

    abstract::The major merozoite-piroplasm surface antigen (mMPSA) of Theileria annulata, Tams1, is known to be antigenically diverse. The possession of variable N-linked glycosylation sites and removal of monoclonal antibody 5E1 reactivity by mild periodate treatment suggested, previously, that divergent epitopes may be conferred...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Katzer F,McKellar S,Ferguson MA,d'Oliveira C,Shiels BR

    更新日期:2002-06-01 00:00:00

  • Expression and substrate specificity of a recombinant cysteine proteinase B of Leishmania braziliensis.

    abstract::The cysteine proteinase B of Leishmania parasites is an important virulence factor. In this study we have expressed, isolated and characterized for the first time a recombinant CPB from Leishmania braziliensis, the causative agent of mucocutaneous leishmaniosis. The mature region of the recombinant CPB shares a high p...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Lanfranco MF,Loayza-Muro R,Clark D,Núñez R,Zavaleta AI,Jimenez M,Meldal M,Coombs GH,Mottram JC,Izidoro M,Juliano MA,Juliano L,Arévalo J

    更新日期:2008-10-01 00:00:00

  • Polymorphism of proteins in malaria parasites following mefloquine treatment.

    abstract::Proteins in malaria parasites (Plasmodium falciparum) isolated from a patient in Thailand before treatment, and after recrudescence of infection subsequent to mefloquine treatment, were compared by two dimensional polyacrylamide gel electrophoresis (2D-PAGE) analysis. Nine 'pre-treatment' and six 'recrudescent' clones...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Pinswasdi C,Thaithong S,Beale GH,Fenton B,Webster HK,Pavanand K

    更新日期:1987-03-01 00:00:00

  • Trypanosoma cruzi poly-zinc finger protein: a novel DNA/RNA-binding CCHC-zinc finger protein.

    abstract::A poly-zinc finger protein, designated PZFP1 was identified in Trypanosoma cruzi for the first time. The protein has 191 amino acids, contains seven motifs Cys(X)(2)Cys(X)(4)His(X)(4)Cys. A recombinant PZFP1 was generated in E. coli and the expected 21kDa polypeptide co-purified with two other inducible products of ab...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Espinosa JM,Portal D,Lobo GS,Pereira CA,Alonso GD,Gómez EB,Lan GH,Pomar RV,Flawiá MM,Torres HN

    更新日期:2003-09-01 00:00:00

  • The interaction of artemisinin with malarial hemozoin.

    abstract::[14C]Artemisinin was taken up by Plasmodium falciparum in culture and concentrated in hemozoin. In vitro, hemin and artemisinin were found to undergo a chemical reaction forming two major products which were isolated by high-performance liquid chromatography (HPLC). The m/z values of the two products were 856 and 871....

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hong YL,Yang YZ,Meshnick SR

    更新日期:1994-01-01 00:00:00

  • SYBR, TaqMan, or both: highly sensitive, non-invasive detection of Cardicola blood fluke species in Southern Bluefin Tuna (Thunnus maccoyii).

    abstract::Three species of blood fluke from the genus Cardicola are known to parasitize and cause disease in Bluefin Tunas--C. forsteri, C. orientalis, and C. opisthorchis. Although initially believed to be separated by geography and host specificity, recent identification of at least two Cardicola spp. concurrently present wit...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Polinski M,Hamilton DB,Nowak B,Bridle A

    更新日期:2013-09-01 00:00:00