Characterization of the gene family encoding a host-protective antigen of the tapeworm Taenia ovis.


:Genomic structure has been determined for a gene encoding a host-protective antigen of the parasitic platyhelminth Taenia ovis. An incomplete cDNA, known as 45W, encodes the protective antigen. Southern hybridisation experiments using 45W cDNA as a probe, revealed that the 45W gene was a member of a multigene family. Differential Southern hybridisation and rapid amplification of cDNA end (RACE) experiments were used to characterise the related genes, allowing the full-length coding region of the 45W encoded antigen to be determined. The gene family comprises a minimum of four members per haploid genome with each member showing varying degrees of 5' and 3' homology with respect to the 45W cDNA. A close homologue of the 45W gene, designated 45S, differed from 45W at 11 of 985 nt comprising the full-length mRNA. Sequencing of several independent RACE products for both 45W and 45S identified a cDNA which may be a product of homologous recombination between these genes, suggesting that the two genes may be alleles. Homologous recombination in genes which encode a host protective antigen such as 45W would provide a mechanism by which antigenic variants could arise.


Mol Biochem Parasitol


Waterkeyn JG,Lightowlers MW,Coppel R,Cowman AF




Has Abstract


1995-07-01 00:00:00














  • Biochemical and functional characterization of CpMuc4, a Cryptosporidium surface antigen that binds to host epithelial cells.

    abstract::Cryptosporidium spp. are intracellular apicomplexan parasites that cause outbreaks of waterborne diarrheal disease worldwide. Previous studies had identified a Cryptosporidium parvum sporozoite antigen, CpMuc4, that appeared to be involved in attachment and invasion of the parasite into intestinal epithelial cells. Cp...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Paluszynski J,Monahan Z,Williams M,Lai O,Morris C,Burns P,O'Connor R

    更新日期:2014-02-01 00:00:00

  • Nuclear actin-related protein is required for chromosome segregation in Toxoplasma gondii.

    abstract::Apicomplexa parasites use complex cell cycles to replicate that are not well understood mechanistically. We have established a robust forward genetic strategy to identify the essential components of parasite cell division. Here we describe a novel temperature sensitive Toxoplasma strain, mutant 13-20C2, which growth a...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Suvorova ES,Lehmann MM,Kratzer S,White MW

    更新日期:2012-01-01 00:00:00

  • Transient transfection and expression of foreign and endogenous genes in the intracellular stages of Trypanosoma cruzi.

    abstract::The capacity for rapid localization of epitope-tagged or fluorescent fusion proteins in cells is an important tool for biological discovery and functional analysis. For Trypanosoma cruzi, the protozoan parasite that causes human Chagas disease, visualization of ectopically-expressed proteins in the clinically-relevant...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Padmanabhan PK,Polidoro RB,Barteneva NS,Gazzinelli RT,Burleigh BA

    更新日期:2014-12-01 00:00:00

  • Benznidazole-resistance in Trypanosoma cruzi: evidence that distinct mechanisms can act in concert.

    abstract::Benznidazole is the main drug used to treat Trypanosoma cruzi infections. However, frequent instances of treatment failure have been reported. To better understand potential resistance mechanisms, we analysed three clones isolated from a single parasite population that had undergone benznidazole-selection. These clone...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Campos MC,Leon LL,Taylor MC,Kelly JM

    更新日期:2014-01-01 00:00:00

  • Genomic organization, chromosomal location and transcription of dispersed and repeated tubulin genes in Leishmania major.

    abstract::The alpha and beta tubulin genes of Leishmania major were cloned and used to study the genomic organization, chromosomal location and transcription of tubulin genes in L. major. The number of beta tubulin isogenes was determined by hybridization of probes representing the 5' and 3' ends of the cloned beta tubulin cDNA...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Spithill TW,Samaras N

    更新日期:1987-05-01 00:00:00

  • Generation and analysis of expressed sequence tags from Trypanosoma cruzi trypomastigote and amastigote cDNA libraries.

    abstract::We have generated 2771 expressed sequence tags (ESTs) from two cDNA libraries of Trypanosoma cruzi CL-Brener. The libraries were constructed from trypomastigote and amastigotes, using a spliced leader primer to synthesize the cDNA second strand, thus selecting for full-length cDNAs. Since the libraries were not normal...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Agüero F,Abdellah KB,Tekiel V,Sánchez DO,González A

    更新日期:2004-08-01 00:00:00

  • Crystal structures of Toxoplasma gondii pterin-4a-carbinolamine dehydratase and comparisons with mammalian and parasite orthologues.

    abstract::The enzyme pterin-4a-carbinolamine dehydratase (PCD) is important for the recycling of pterins within eukaryotic cells. A recombinant expression system for PCD from the apicomplexan parasite Toxoplasma gondii has been prepared, the protein purified and crystallised. Single crystal X-ray diffraction methods have produc...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Cameron S,Fyffe SA,Goldie S,Hunter WN

    更新日期:2008-04-01 00:00:00

  • Purification, characterization, and immunochemical studies of beta-N-acetyl-D-hexosaminidase from the parasitic nematode Trichinella spiralis.

    abstract::The exoglycosidase, beta-N-acetyl-D-hexosaminidase was purified 600-fold from the muscle-stage larvae (L1) of Trichinella spiralis. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), the purified enzyme-active fraction contained 4 polypeptides with apparent molecular weights of 100,000, 68,000, 5...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Rhoads ML

    更新日期:1988-10-01 00:00:00

  • Validation of a modified method for Bxb1 mycobacteriophage integrase-mediated recombination in Plasmodium falciparum by localization of the H-protein of the glycine cleavage complex to the mitochondrion.

    abstract::The glycine cleavage complex (GCV) is a potential source of the one carbon donor 5,10-methylene-tetrahydrofolate (5,10-CH(2)-THF) in the malaria parasite Plasmodium falciparum. One carbon (C1) donor units are necessary for amino acid and nucleotide biosynthesis, and for the initiation of mitochondrial and plastid tran...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Spalding MD,Allary M,Gallagher JR,Prigge ST

    更新日期:2010-08-01 00:00:00

  • Homologous telomeric sequences are present in different species of the genus Plasmodium.

    abstract::The telomeric sequence cloned from Plasmodium berghei (see M. Ponzi et al. (1985) EMBO J. 4, 2991-2995) was tested for species specificity. A telomeric and a subtelomeric fragment of the cloned insert served as separate, labelled probes on pulsed field gradient electrophoretical patterns and on genomic digests from th...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Dore E,Pace T,Ponzi M,Scotti R,Frontali C

    更新日期:1986-11-01 00:00:00

  • Role of glutathione in the detoxification of ferriprotoporphyrin IX in chloroquine resistant Plasmodium berghei.

    abstract::The reduction in hemozoin content is a well known feature of chloroquine-resistant Plasmodium berghei. Using NK65-derived lines displaying increasing resistance levels, we observed an inverse relationship between the hemozoin content, and the glutathione (GSH) and glutathione S-transferase (GST) levels. Treatment of h...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Platel DF,Mangou F,Tribouley-Duret J

    更新日期:1999-01-25 00:00:00

  • Thiol groups on the surface of anaerobic parasitic protozoa.

    abstract::Evidence is presented that Giardia lamblia and Entamoeba histolytica, phylogenetically unrelated aerotolerant anaerobes, have crucial thiol groups on or easily accessible to their external surface. Both parasites were killed by three structurally unrelated thiol-blocking reagents which penetrate intact cells poorly or...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Gillin FD,Reiner DS,Levy RB,Henkart PA

    更新日期:1984-09-01 00:00:00

  • Surface epitope localization and gene structure of a Babesia bovis 44-kilodalton variable merozoite surface antigen.

    abstract::Variation of Babesia bovis merozoite surface antigens occurs among geographic strains of the parasite. In this and a concurrent report, we investigate this variation at the gene and protein level. Using a monoclonal antibody (mAb 23/70.174), B. bovis gene sequences were identified that encoded a surface epitope of a 4...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Jasmer DP,Reduker DW,Hines SA,Perryman LE,McGuire TC

    更新日期:1992-10-01 00:00:00

  • Expression and functional characterization of a giant Type I fatty acid synthase (CpFAS1) gene from Cryptosporidium parvum.

    abstract::A 25-kb CpFAS1 gene from Cryptosporidium parvum has been engineered and expressed as five individual maltose-binding protein (MBP)-fusion proteins: an N-terminal loading unit, three fatty acyl elongation modules, and a C-terminal reductase. Enzymatic activities of all domains (except the reductase) were individually a...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Zhu G,Li Y,Cai X,Millership JJ,Marchewka MJ,Keithly JS

    更新日期:2004-03-01 00:00:00

  • The complexity of the secreted NPA and FAR lipid-binding protein families of Haemonchus contortus revealed by an iterative proteomics-bioinformatics approach.

    abstract::Two different classes of small nematode specific lipid-binding proteins, the nematode polyprotein allergens/antigens (NPAs) and the fatty acid- and retinol-binding (FAR) proteins, are secreted by helminth parasites. Until now, there was no evidence of the expression or secretion of these two families of proteins in Ha...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kuang L,Colgrave ML,Bagnall NH,Knox MR,Qian M,Wijffels G

    更新日期:2009-11-01 00:00:00

  • Sequences required for the flagellar targeting of an integral membrane protein.

    abstract::Previous studies have established that the ISO1 glucose transporter of Leishmania enriettii resides primarily in the flagellar membrane, whereas the ISO2 glucose transporter is located in the pellicular plasma membrane surrounding the cell body. This pronounced difference in subcellular targeting is conferred by the N...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Nasser MI,Landfear SM

    更新日期:2004-05-01 00:00:00

  • Preliminary characterization of the major RNA species from Plasmodium falciparum.

    abstract::Polyacrylamide gel electrophoresis of the total RNA extracted from cultures of Plasmodium falciparum resolved two major RNA components with estimated molecular weights of 1.3 X 10(6) and 0.72 - 0.74 X 10(6). Oligo(dT)-cellulose column chromatography of the total cellular RNA indicates that more than 90% of the total r...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Vezza AC,Trager W

    更新日期:1981-12-01 00:00:00

  • Uptake of the trypanocidal drug suramin by bloodstream forms of Trypanosoma brucei and its effect on respiration and growth rate in vivo.

    abstract::After a single intravenous injection of suramin the rate of removal of the drug from the plasma into other tissue compartments of the rat is independent of initial concentration. The data can be fitted to the sum of two exponential functions, consistent with a two-compartment, open model system. Trypanosomes take up o...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Fairlamb AH,Bowman IB

    更新日期:1980-10-01 00:00:00

  • SYBR, TaqMan, or both: highly sensitive, non-invasive detection of Cardicola blood fluke species in Southern Bluefin Tuna (Thunnus maccoyii).

    abstract::Three species of blood fluke from the genus Cardicola are known to parasitize and cause disease in Bluefin Tunas--C. forsteri, C. orientalis, and C. opisthorchis. Although initially believed to be separated by geography and host specificity, recent identification of at least two Cardicola spp. concurrently present wit...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Polinski M,Hamilton DB,Nowak B,Bridle A

    更新日期:2013-09-01 00:00:00

  • mRNA sequences for Haemonchus contortus intestinal cathepsin B-like cysteine proteases display an extreme in abundance and diversity compared with other adult mammalian parasitic nematodes.

    abstract::Cathepsin B-like cysteine protease (cbl) genes produce the most abundant mRNAs ( approximately 16%) detected in the adult female intestine of the parasitic nematode Haemonchus contortus. CBL enzymes appear to digest host proteins and are vaccine candidates for immune control of H. contortus and potentially other paras...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Jasmer DP,Mitreva MD,McCarter JP

    更新日期:2004-10-01 00:00:00

  • Stable transfection and continuous expression of heterologous genes in Entamoeba invadens.

    abstract::Amoebiasis is spread by the ingestion of dormant Entamoeba histolytica cysts. Intervention of encystation could break the transmission cycle, thereby reducing disease burden. The model system used to study trophozoite to cyst differentiation is Entamoeba invadens. Here we describe an electroporation-based method for s...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Singh N,Ojha S,Bhattacharya A,Bhattacharya S

    更新日期:2012-07-01 00:00:00

  • Profiling the malaria genome: a gene survey of three species of malaria parasite with comparison to other apicomplexan species.

    abstract::We have undertaken the first comparative pilot gene discovery analysis of approximately 25,000 random genomic and expressed sequence tags (ESTs) from three species of Plasmodium, the infectious agent that causes malaria. A total of 5482 genome survey sequences (GSSs) and 5582 ESTs were generated from mung bean nucleas...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Carlton JM,Muller R,Yowell CA,Fluegge MR,Sturrock KA,Pritt JR,Vargas-Serrato E,Galinski MR,Barnwell JW,Mulder N,Kanapin A,Cawley SE,Hide WA,Dame JB

    更新日期:2001-12-01 00:00:00

  • Identification and functional characterization of a member of the PUR-alpha family from Schistosoma mansoni.

    abstract::Schistosoma mansoni p14 gene encodes an eggshell precursor that is expressed only in vitelline cells of mature female worms in response to a male stimulus. The upstream region of the p14 gene contains several potential cis-acting regulatory sequences. We used the upstream region of the p14 gene as bait in a yeast-one-...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Fantappie MR,Osman A,Niles EG,LoVerde PT

    更新日期:2000-10-01 00:00:00

  • Rapid evolution of an erythrocyte invasion gene family: the Plasmodium reichenowi Reticulocyte Binding Like (RBL) genes.

    abstract::Malarial merozoites use an array of ligands, including members of the Reticulocyte Binding Like (RBL) super-family of invasion proteins, to identify and invade erythrocytes. RBL family members are large Type I membrane anchored proteins expressed at the invasive end of merozoites that share homology with the Reticuloc...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Rayner JC,Huber CS,Galinski MR,Barnwell JW

    更新日期:2004-02-01 00:00:00

  • Molecular cloning and characterization of a C-type lectin from Ancylostoma ceylanicum: evidence for a role in hookworm reproductive physiology.

    abstract::Lectins comprise a family of related proteins that mediate essential cell functions through binding to carbohydrates. Within this protein family, C-type lectins are defined by the requirement of calcium for optimal biologic activity. Using reverse transcription PCR, a cDNA corresponding to a putative C-type lectin has...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Brown AC,Harrison LM,Kapulkin W,Jones BF,Sinha A,Savage A,Villalon N,Cappello M

    更新日期:2007-02-01 00:00:00

  • High efficiency transfection of Plasmodium berghei facilitates novel selection procedures.

    abstract::The use of transfection in the study of the biology of malaria parasites has been limited due to poor transfection efficiencies (frequency of 10(-6) to 10(-9)) and a paucity of selection markers. Here, a new method of transfection, using non-viral Nucleofector technology, is described for the rodent parasite Plasmodiu...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Janse CJ,Franke-Fayard B,Mair GR,Ramesar J,Thiel C,Engelmann S,Matuschewski K,van Gemert GJ,Sauerwein RW,Waters AP

    更新日期:2006-01-01 00:00:00

  • Mimicry of elastin repetitive motifs by Theileria annulata sporozoite surface antigen.

    abstract::Theileria annulata is an important pathogen of cattle in the tropics. The gene sequence of a sporozoite surface antigen (SPAG-1) is reported. Data is also presented demonstrating that SPAG-1 is synthesised as a large precursor. This antigen, which is a candidate for inclusion in a subunit vaccine, shows a remarkable d...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hall R,Hunt PD,Carrington M,Simmons D,Williamson S,Mecham RP,Tait A

    更新日期:1992-07-01 00:00:00

  • RNA interference targeting leucine aminopeptidase blocks hatching of Schistosoma mansoni eggs.

    abstract::Schistosoma mansoni leucine aminopeptidase (LAP) is thought to play a central role in hatching of the miracidium from the schistosome egg. We identified two discrete LAPs genes in the S. mansoni genome, and their orthologs in S. japonicum. The similarities in sequence and exon/intron structure of the two genes, LAP1 a...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Rinaldi G,Morales ME,Alrefaei YN,Cancela M,Castillo E,Dalton JP,Tort JF,Brindley PJ

    更新日期:2009-10-01 00:00:00

  • The effect of over-expression of the alternative oxidase in the procyclic forms of Trypanosoma brucei.

    abstract::Trypanosome alternative oxidase (TAO) is the cyanide-resistant but SHAM-sensitive terminal oxidase of the mitochondrial electron transport chain in African trypanosomes. The bloodstream forms of Trypanosoma brucei lack cytochromes and respire exclusively via TAO. On the other hand, the insect, or procyclic form posses...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Walker R Jr,Saha L,Hill GC,Chaudhuri M

    更新日期:2005-02-01 00:00:00

  • Duplication, gene conversion, and genetic diversity in the species-specific acyl-CoA synthetase gene family of Plasmodium falciparum.

    abstract::While genes encoding antigens and other highly polymorphic proteins are commonly found in subtelomeres, it is unusual to find a small family of housekeeping genes in these regions. We found that in the species Plasmodium falciparum only, a non-subtelomeric acyl-CoA synthetase (ACS) gene has expanded into a family of d...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Bethke LL,Zilversmit M,Nielsen K,Daily J,Volkman SK,Ndiaye D,Lozovsky ER,Hartl DL,Wirth DF

    更新日期:2006-11-01 00:00:00