Characterization of the gene family encoding a host-protective antigen of the tapeworm Taenia ovis.

abstract：:Telomeric DNA of trypanosomatids possesses a modified thymine base, called base J, that is synthesized in a two-step process; the base is hydroxylated by a thymidine hydroxylase forming hydroxymethyluracil (hmU) and a glucose moiety is then attached by the J-associated glucosyltransferase (JGT). To examine the importa...

journal_title：Molecular and biochemical parasitology

authors： Bullard W,Cliffe L,Wang P,Wang Y,Sabatini R

更新日期：2015-12-01 00:00:00

abstract：:The capacity for rapid localization of epitope-tagged or fluorescent fusion proteins in cells is an important tool for biological discovery and functional analysis. For Trypanosoma cruzi, the protozoan parasite that causes human Chagas disease, visualization of ectopically-expressed proteins in the clinically-relevant...

journal_title：Molecular and biochemical parasitology

authors： Padmanabhan PK,Polidoro RB,Barteneva NS,Gazzinelli RT,Burleigh BA

更新日期：2014-12-01 00:00:00

abstract：:Soon after infecting a mammalian host, cercariae of Schistosoma mansoni rapidly undergo a series of morphologic and biochemical adaptations associated with transformation to their next developmental stage, the schistosomulum. Few of these changes are associated with alterations in gene expression except for an apparen...

journal_title：Molecular and biochemical parasitology

authors： Blanton RE,Licate LS

更新日期：1992-04-01 00:00:00

abstract：:Repetitive DNA sequences of the Leishmania donovani genome have been identified by screening a recombinant DNA library made by cloning sheared genomic DNA into the vector pAT153. Bacterial clones containing a highly repetitive DNA sequence have been isolated. DNA sequencing has shown that this sequence is composed of ...

journal_title：Molecular and biochemical parasitology

authors： Ellis J,Crampton J

更新日期：1988-05-01 00:00:00

abstract：:Plasmodium falciparum invades human erythrocytes by redundant pathways. Unlike Plasmodium vivax that has one Duffy Binding-Like (DBL) receptor, P. falciparum has four members of the DBL receptor family. Furthermore, one of these DBL genes, BAEBL, has polymorphisms at four amino acids in region II; each polymorphism bi...

journal_title：Molecular and biochemical parasitology

authors： Jiang L,Duriseti S,Sun P,Miller LH

更新日期：2009-11-01 00:00:00

abstract：:Adult Schistosoma mansoni worms rapidly degrade their endogenous glycogen stores immediately after isolation from the host. In NCTC 109 or in a diphasic culture medium the glycogen levels slowly recovered again after the initial decrease. The rapid degradation of glycogen could be prevented, even in a simple salt medi...

journal_title：Molecular and biochemical parasitology

authors： Tielens AG,van den Bergh SG

更新日期：1987-07-01 00:00:00

abstract：:The FMRFamide-related peptides (FaRPs), KHEYLRFamide (AF2) and KSAYMRFamide (PF3) were structurally characterised from the parasitic nematode of sheep, Haemonchus contortus (MH isolate). Both peptides were sequenced in a single gas-phase sequencing run and their structure confirmed by mass spectrometry which identifie...

journal_title：Molecular and biochemical parasitology

authors： Marks NJ,Sangster NC,Maule AG,Halton DW,Thompson DP,Geary TG,Shaw C

更新日期：1999-05-25 00:00:00

abstract：:A polyprotein composed of multiple units arranged in direct tandem arrays has been identified in parasitic and free living nematodes. Analysis of previously cloned units from the Dirofilaria immitis polyprotein antigen (DiPA) indicated the units were nearly identical but here we demonstrate that they segregate into tw...

journal_title：Molecular and biochemical parasitology

authors： Poole CB,Hornstra LJ,Benner JS,Fink JR,McReynolds LA

更新日期：1996-11-12 00:00:00

abstract：:Two different classes of small nematode specific lipid-binding proteins, the nematode polyprotein allergens/antigens (NPAs) and the fatty acid- and retinol-binding (FAR) proteins, are secreted by helminth parasites. Until now, there was no evidence of the expression or secretion of these two families of proteins in Ha...

journal_title：Molecular and biochemical parasitology

authors： Kuang L,Colgrave ML,Bagnall NH,Knox MR,Qian M,Wijffels G

更新日期：2009-11-01 00:00:00

abstract：:Recent investigations into quinoline and phenanthrene methanol resistance in Plasmodium falciparum have described a linkage between amplification of the mdr homologue pfmdr1 and decreased susceptibility to mefloquine (MQ) and halofantrine (HF). We have examined the current theories on cross-resistance patterns and pfm...

journal_title：Molecular and biochemical parasitology

authors： Ritchie GY,Mungthin M,Green JE,Bray PG,Hawley SR,Ward SA

更新日期：1996-12-02 00:00:00

abstract：:Trypanosoma brucei is a unicellular parasite that is transmitted from one mammalian host to the next by tsetse flies. The expression of many trypanosome genes is regulated during the life cycle but there is no evidence for developmental control of transcription by RNA polymerase II. T. brucei expresses at least two he...

journal_title：Molecular and biochemical parasitology

authors： Hotz HR,Lorenz P,Fischer R,Krieger S,Clayton C

更新日期：1995-12-01 00:00:00

abstract：:Nucleotide-sugar transporters (NSTs) transport activated sugars (e.g. UDP-GlcNAc) from the cytosol to the lumen of the endoplasmic reticulum or Golgi apparatus where they are used to make glycoproteins and glycolipids. UDP-Glc is an important component of the N-glycan-dependent quality control (QC) system for protein ...

journal_title：Molecular and biochemical parasitology

authors： Banerjee S,Cui J,Robbins PW,Samuelson J

更新日期：2008-05-01 00:00:00

abstract：:The cyclophilins are a large family of proteins implicated in folding, transport and regulation of other proteins and are potential drug targets in cancer and in some viral and parasitic infections. The functionality of cyclophilins appears to depend on peptidyl-prolyl cis-trans isomerase (foldase) and/or molecular ch...

journal_title：Molecular and biochemical parasitology

authors： Marín-Menéndez A,Monaghan P,Bell A

更新日期：2012-07-01 00:00:00

abstract：:Cathepsin L-like activity was demonstrated in the excretory/secretory (E/S) products of Fasciola hepatica newly excysted juveniles (NEJ), 3-week-old, 5-week-old and mature flukes using the fluorogenic substituted 7-amino-4-methylcoumarin substrates Z-phe-arg-AMC, Z-arg-arg-AMC and Z-arg-AMC. Gelatin-substrate polyacry...

journal_title：Molecular and biochemical parasitology

authors： Carmona C,Dowd AJ,Smith AM,Dalton JP

更新日期：1993-11-01 00:00:00

abstract：:Trophozoites of Entamoeba invadens were able to ingest glucopolysaccharides and metabolize them. An activity capable of degrading these substrates was purified to apparent homogeneity. The enzyme was identified as beta-amylase (alpha-1,4-D-glucan maltohydrolase EC 3.2.1.2): It was active against glycogen, amylose and ...

journal_title：Molecular and biochemical parasitology

authors： Serafimova F,Franz A,Werries E

更新日期：1996-12-20 00:00:00

abstract：:Phosphoinositides comprise a group of essential phospholipids that control a variety of cellular functions. In the case of the human malaria parasite Plasmodium falciparum, phosphoinositides have been shown to trigger exflagellation and to affect haemoglobin endocytosis and maturation of the parasite's digestive vacuo...

journal_title：Molecular and biochemical parasitology

authors： Krüger T,Sanchez CP,Lanzer M

更新日期：2010-08-01 00:00:00

abstract：:Adults of Onchocerca volvulus and Onchocerca gibsoni were identically fractionated into a surface-enriched fraction, a phosphate buffered saline (PBS) extract and a PBS insoluble-detergent soluble fraction. Glycoproteins were prepared from these extracts and all fractions were examined by the Western blot technique us...

journal_title：Molecular and biochemical parasitology

authors： Cabrera Z,Parkhouse RM

更新日期：1986-09-01 00:00:00

abstract：:We report the isolation and sequence of a part of a gene encoding a collagen from the genome of the human filarial parasite Brugia malayi. The deduced amino acid sequence of the sequenced portion of this gene, which we have designated BmCol1, differs from the most catalogued nematode collagens in that it is composed p...

journal_title：Molecular and biochemical parasitology

authors： Caulagi VR,Werner C,Rajan TV

更新日期：1991-03-01 00:00:00

abstract：:Several proteins synthesized by mature asexual stages of Plasmodium falciparum interact with the erythrocyte membrane skeleton. One of these is the mature-parasite-infected erythrocyte surface antigen (MESA; also called PfEMP2), a phosphoprotein of 250-300 kDa, which is found on the internal face of the erythrocyte me...

journal_title：Molecular and biochemical parasitology

authors： Lustigman S,Anders RF,Brown GV,Coppel RL

更新日期：1990-01-15 00:00:00

abstract：:Given the growing appreciation of serious health sequelae from widespread Trichomonas vaginalis infection, new tools are needed to study the parasite's genetic diversity. To this end we have identified and characterized a panel of 21 microsatellites and six single-copy genes from the T. vaginalis genome, using seven l...

journal_title：Molecular and biochemical parasitology

authors： Conrad M,Zubacova Z,Dunn LA,Upcroft J,Sullivan SA,Tachezy J,Carlton JM

更新日期：2011-01-01 00:00:00

abstract：:Cryptosporidium parvum is a protozoan parasite of the intestinal epithelium that has caused numerous outbreaks of diarrheal illness in humans. During our studies of the host immune response to C. parvum infection, we noted that two of the immunodominant surface antigens of the sporozoite stage of the parasite readily ...

journal_title：Molecular and biochemical parasitology

authors： Priest JW,Xie LT,Arrowood MJ,Lammie PJ

更新日期：2001-03-01 00:00:00

abstract：:We have examined changes in the relative synthesis of individual proteins in promastigotes of Leishmania major subjected to decreasing serum levels in vitro. We observed increases in the relative synthesis of the putative heat-shock proteins of 82 and 70 kDa and of proteins of 79 and 41 kDa but decreases in the synthe...

journal_title：Molecular and biochemical parasitology

authors： Toye P,Remold H

更新日期：1989-06-01 00:00:00

abstract：:Toxoplasma gondii differentially expresses two forms of lactate dehydrogenase in tachyzoites and bradyzoites, respectively, designated LDH1 and LDH2. Previously it was demonstrated that LDH1 and LDH2 share a unique structural feature with LDH from the malarial parasite Plasmodium falciparum (pLDH), namely, the additio...

journal_title：Molecular and biochemical parasitology

authors： Dando C,Schroeder ER,Hunsaker LA,Deck LM,Royer RE,Zhou X,Parmley SF,Vander Jagt DL

更新日期：2001-11-01 00:00:00

abstract：:Opisthorchis viverrini is the causative agent of human opisthorchiasis in Thailand and long lasting infection with the parasite has been correlated with the development of cholangiocarcinoma. In this work we have molecularly characterized the first member of a protein family carrying two DM9 repeats in this parasite (...

journal_title：Molecular and biochemical parasitology

authors： Labbunruang N,Phadungsil W,Tesana S,Smooker PM,Grams R

更新日期：2016-05-01 00:00:00

abstract：:Thrombospondin-related anonymous protein (TRAP) family members participate in attachment and invasion of host cells by apicomplexan parasites. A TRAP homologue in Neospora caninum strain Nc-1 (NcMIC2) was cloned, sequenced and found to be 61% identical (75% similar) at the amino acid level to Toxoplasma gondii MIC2 (T...

journal_title：Molecular and biochemical parasitology

authors： Lovett JL,Howe DK,Sibley LD

更新日期：2000-03-15 00:00:00

abstract：:Schistosomiasis remains a largely neglected, global health problem. The morbid pathology of the disease stems from the host's inflammatory response to parasite eggs trapped in host tissues. Long term host/parasite survival is dependent upon the successful modulation of the acute pathological response, which is induced...

journal_title：Molecular and biochemical parasitology

authors： Cass CL,Johnson JR,Califf LL,Xu T,Hernandez HJ,Stadecker MJ,Yates JR 3rd,Williams DL

更新日期：2007-10-01 00:00:00

abstract：:We have undertaken the first comparative pilot gene discovery analysis of approximately 25,000 random genomic and expressed sequence tags (ESTs) from three species of Plasmodium, the infectious agent that causes malaria. A total of 5482 genome survey sequences (GSSs) and 5582 ESTs were generated from mung bean nucleas...

journal_title：Molecular and biochemical parasitology

authors： Carlton JM,Muller R,Yowell CA,Fluegge MR,Sturrock KA,Pritt JR,Vargas-Serrato E,Galinski MR,Barnwell JW,Mulder N,Kanapin A,Cawley SE,Hide WA,Dame JB

更新日期：2001-12-01 00:00:00

abstract：:A poly-zinc finger protein, designated PZFP1 was identified in Trypanosoma cruzi for the first time. The protein has 191 amino acids, contains seven motifs Cys(X)(2)Cys(X)(4)His(X)(4)Cys. A recombinant PZFP1 was generated in E. coli and the expected 21kDa polypeptide co-purified with two other inducible products of ab...

journal_title：Molecular and biochemical parasitology

authors： Espinosa JM,Portal D,Lobo GS,Pereira CA,Alonso GD,Gómez EB,Lan GH,Pomar RV,Flawiá MM,Torres HN

更新日期：2003-09-01 00:00:00

abstract：:Protein kinase activities in bloodstream and procyclic forms of Trypanosoma brucei have been partially purified and characterised. Cytosolic extracts were separated on DEAE-cellulose and assayed for the ability to phosphorylate histone in the presence of Ca2+ and diacylglycerol. Five peaks of activity were identified ...

journal_title：Molecular and biochemical parasitology

authors： Keith K,Hide G,Tait A

更新日期：1990-11-01 00:00:00

abstract：:The genus Cryptosporidium includes several species of intestinal protozoan parasites which multiply in intestinal epithelial cells. The impact of this infection on the transcriptome of cultured host cells was investigated using DNA microarray hybridizations. The expression of 14 genes found to be consistently up- or d...

journal_title：Molecular and biochemical parasitology

authors： Yang YL,Serrano MG,Sheoran AS,Manque PA,Buck GA,Widmer G

更新日期：2009-11-01 00:00:00