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Microsatellite polymorphism in the sexually transmitted human pathogen Trichomonas vaginalis indicates a genetically diverse parasite.

Abstract：

:Given the growing appreciation of serious health sequelae from widespread Trichomonas vaginalis infection, new tools are needed to study the parasite's genetic diversity. To this end we have identified and characterized a panel of 21 microsatellites and six single-copy genes from the T. vaginalis genome, using seven laboratory strains of diverse origin. We have (1) adapted our microsatellite typing method to incorporate affordable fluorescent labeling, (2) determined that the microsatellite loci remain stable in parasites continuously cultured for up to 17 months, and (3) evaluated microsatellite marker coverage of the six chromosomes that comprise the T. vaginalis genome, using fluorescent in situ hybridization (FISH). We have used the markers to show that T. vaginalis is a genetically diverse parasite in a population of commonly used laboratory strains. In addition, we have used phylogenetic methods to infer evolutionary relationships from our markers in order to validate their utility in future population analyses. Our panel is the first series of robust polymorphic genetic markers for T. vaginalis that can be used to classify and monitor lab strains, as well as provide a means to measure the genetic diversity and population structure of extant and future T. vaginalis isolates.

journal_name

Mol Biochem Parasitol

journal_title

Molecular and biochemical parasitology

authors

Conrad M,Zubacova Z,Dunn LA,Upcroft J,Sullivan SA,Tachezy J,Carlton JM

doi

10.1016/j.molbiopara.2010.08.006

subject

Has Abstract

pub_date

2011-01-01 00:00:00

pages

30-8

issue

1

eissn

0166-6851

issn

1872-9428

pii

S0166-6851(10)00229-X

journal_volume

175

pub_type

杂志文章

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