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Trypanosoma cruzi poly-zinc finger protein: a novel DNA/RNA-binding CCHC-zinc finger protein.

Abstract：

:A poly-zinc finger protein, designated PZFP1 was identified in Trypanosoma cruzi for the first time. The protein has 191 amino acids, contains seven motifs Cys(X)(2)Cys(X)(4)His(X)(4)Cys. A recombinant PZFP1 was generated in E. coli and the expected 21kDa polypeptide co-purified with two other inducible products of about 42 and 63kDa. Western blot analysis of cell extracts using an anti-PZFP1 antibody recognized a major band of 41kDa. Electrophoretic mobility shift analysis demonstrated that both, recombinant and native PZFP1, specifically interact with single-stranded DNA or RNA oligonucleotides carrying recognition sequences of other CCHC proteins. The protein was localized mainly in the cytoplasm and nucleus as observed by indirect immunofluorescence analysis. PZFP1 interacted specifically with a T. cruzi serine-arginine-rich protein (TcSR) in a yeast two-hybrid assay, suggesting a role in pre-mRNA processing.

journal_name

Mol Biochem Parasitol

journal_title

Molecular and biochemical parasitology

authors

Espinosa JM,Portal D,Lobo GS,Pereira CA,Alonso GD,Gómez EB,Lan GH,Pomar RV,Flawiá MM,Torres HN

doi

10.1016/s0166-6851(03)00187-7

subject

Has Abstract

pub_date

2003-09-01 00:00:00

pages

35-44

issue

1

eissn

0166-6851

issn

1872-9428

pii

S0166685103001877

journal_volume

131

pub_type

杂志文章

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