Polymorphism of proteins in malaria parasites following mefloquine treatment.

abstract：:Phosphoinositides comprise a group of essential phospholipids that control a variety of cellular functions. In the case of the human malaria parasite Plasmodium falciparum, phosphoinositides have been shown to trigger exflagellation and to affect haemoglobin endocytosis and maturation of the parasite's digestive vacuo...

journal_title：Molecular and biochemical parasitology

authors： Krüger T,Sanchez CP,Lanzer M

更新日期：2010-08-01 00:00:00

abstract：:In most organisms rDNA transcription ceases under conditions of growth stress. However, we have earlier shown that pre-rRNA accumulates during encystation in Entamoeba invadens. We labeled newly-synthesized rRNA during encystation, with [methyl-(3)H] methionine in the presence of chitinase to enable uptake of isotope....

journal_title：Molecular and biochemical parasitology

authors： Ojha S,Ahamad J,Bhattacharya A,Bhattacharya S

更新日期：2014-06-01 00:00:00

abstract：:The tick-borne protozoan parasite, Babesia bovis is one of the causes of bovine babesiosis, an economically important disease of cattle in tropical and sub-tropical countries. Using the recently published genome sequence of the parasite, we developed a panel of eight mini- and micro-satellite markers and used these to...

journal_title：Molecular and biochemical parasitology

authors： Simuunza M,Bilgic H,Karagenc T,Syakalima M,Shiels B,Tait A,Weir W

更新日期：2011-06-01 00:00:00

abstract：:During intracellular development of the malarial parasite numerous membranous vesicles appear in the infected erythrocyte cytoplasm between the parasitophorous vacuolar membrane (PVM) and the erythrocyte plasma membrane. In this study we describe the characterization of a monoclonal antibody which recognizes two major...

journal_title：Molecular and biochemical parasitology

authors： Martinez SL,Clavijo CA,Winograd E

更新日期：1998-03-15 00:00:00

abstract：:Erythrocytes from Plasmodium berghei-infected mice on incubation either in plasma or artificial isotonic media showed an increase in uptake of 45Ca2+ compared with erythrocytes from uninfected mice. Infected cells (55% parasitaemia) incubated in plasma from normal or infected mice gave uptake rates of 9.8 and 8.1 nmol...

journal_title：Molecular and biochemical parasitology

authors： Krungkrai J,Yuthavong Y

更新日期：1983-03-01 00:00:00

abstract：:MRE11 is a conserved multi-functional protein that is important for maintaining genomic integrity in yeast and mammalian cells. By database searching, we identified a full-length candidate MRE11 on Trypanosoma brucei chromosome II. We subsequently cloned and sequenced the corresponding gene from the Lister 427 strain....

journal_title：Molecular and biochemical parasitology

authors： Tan KS,Leal ST,Cross GA

更新日期：2002-11-01 00:00:00

abstract：:Trypanosoma brucei survives in mammals by antigenic variation of its surface coat consisting of variant surface glycoprotein (VSG). Trypanosomes change coat mainly by replacing the transcribed VSG genes in an active telomeric expression site by a different VSG gene. There are about 20 different expression sites and tr...

journal_title：Molecular and biochemical parasitology

authors： Borst P,Ulbert S

更新日期：2001-04-25 00:00:00

abstract：:Many of the currently available nematicides used in nematode control are hazardous to the user, environment and beneficial non-target organisms. Therefore the need to develop alternative methods for nematode control such as the development of nematode-resistant crops through RNA-mediated interference (RNAi) holds grea...

journal_title：Molecular and biochemical parasitology

authors： Joseph S,Gheysen G,Subramaniam K

更新日期：2012-11-01 00:00:00

abstract：:Two proteins were previously described in the excretory-secretory products (ES) collected from Dirofilaria immitis during the molt from the third stage to the fourth stage in vitro. The two proteins were purified using cation exchange and reverse phase HPLC. During the purification of these two proteins, a third prote...

journal_title：Molecular and biochemical parasitology

authors： Frank GR,Grieve RB

更新日期：1996-01-01 00:00:00

abstract：:The intra-species polymorphism of E. histolytica and E. dispar species in endemic area is an important tool for geographic distribution and spread mechanism studies. Since E. dispar and E. histolytica shears ecological niche, cell cycle, and transmission mechanism for human host, we studied the intra-specie variation ...

journal_title：Molecular and biochemical parasitology

authors： Ramos F,García G,Valadez A,Morán P,González E,Gómez A,Melendro EI,Valenzuela O,Ximénez C

更新日期：2005-06-01 00:00:00

abstract：:After a single intravenous injection of suramin the rate of removal of the drug from the plasma into other tissue compartments of the rat is independent of initial concentration. The data can be fitted to the sum of two exponential functions, consistent with a two-compartment, open model system. Trypanosomes take up o...

journal_title：Molecular and biochemical parasitology

authors： Fairlamb AH,Bowman IB

更新日期：1980-10-01 00:00:00

abstract：:Previous studies in the parasitic protist Trichomonas vaginalis have revealed that protein coding genes are transcribed by an alpha-amanitin-resistant RNA polymerase (RNAP) II. To investigate whether this unusual property is a general characteristic of trichomonads, we addressed the physiology of RNA synthesis in lyso...

journal_title：Molecular and biochemical parasitology

authors： Vanácová S,Tachezy J,Ullu E,Tschudi C

更新日期：2001-07-01 00:00:00

abstract：:Species of Centrorhynchus (Polymorphida: Centrorhynchidae) commonly parasitize various falconiform and strigiform birds worldwide. In the present study, the complete mitochondrial (mt) genome sequences of Centrorhynchus clitorideus was sequenced and annotated for the first time based on specimens collected from the li...

journal_title：Molecular and biochemical parasitology

authors： Muhammad N,Suleman,Khan MS,Li L,Zhao Q,Ullah H,Zhu XQ,Ma J

更新日期：2020-05-01 00:00:00

abstract：:The binding of tritiated benzimidazoles (BZs)-albendazole, parbendazole, oxibendazole, mebendazole, oxfendazole and fenbendazole-to crude tubulin extracts from BZ-susceptible and -resistant Haemonchus contortus has been examined. For all BZs, the binding was substantially lower in the resistant isolate. The extent of ...

journal_title：Molecular and biochemical parasitology

authors： Lacey E,Prichard RK

更新日期：1986-05-01 00:00:00

abstract：:Infection with larval trematodes has been shown to inhibit several snail-host defences, including hemocyte phagocytosis, cytotoxicity, motility, and adherence. Certain plasma factors which mediate snail defence responses, and which may be produced by host hemocytes, also appear to be altered by these parasites. In thi...

journal_title：Molecular and biochemical parasitology

authors： Lodes MJ,Connors VA,Yoshino TP

更新日期：1991-11-01 00:00:00

abstract：:The in vitro-released 'excretory/secretory' (ES) and somatic antigens of the third stage (infective) larva of Anisakis simplex were characterised by radioiodination, immunoprecipitation, and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Oral infection with the parasite elicited a substantial IgG antibody...

journal_title：Molecular and biochemical parasitology

authors： Kennedy MW,Tierney J,Ye P,McMonagle FA,McIntosh A,McLaughlin D,Smith JW

更新日期：1988-10-01 00:00:00

abstract：:The glycine cleavage complex (GCV) is a potential source of the one carbon donor 5,10-methylene-tetrahydrofolate (5,10-CH(2)-THF) in the malaria parasite Plasmodium falciparum. One carbon (C1) donor units are necessary for amino acid and nucleotide biosynthesis, and for the initiation of mitochondrial and plastid tran...

journal_title：Molecular and biochemical parasitology

authors： Spalding MD,Allary M,Gallagher JR,Prigge ST

更新日期：2010-08-01 00:00:00

abstract：:This study is the first demonstration of individual gene products synthesized by Hymenolepis diminuta. To identify proteins synthesized specifically by H. diminuta, we radiolabeled adult worms with [14C]leucine in vitro. Subsequent one- and two-dimensional gel electrophoretic separation and staining of the protein con...

journal_title：Molecular and biochemical parasitology

authors： Atkinson BG,Podesta RB

更新日期：1982-07-01 00:00:00

abstract：:We have investigated the protein and glycoprotein content of Plasmodium falciparum merozoites by metabolically labeling cultures of schizont-stage parasites with [35S]methionine or with [3H]glucosamine followed by incubation in nonradioactive medium to allow the schizonts to mature into merozoites, infect new erythroc...

journal_title：Molecular and biochemical parasitology

authors： Howard RF,Reese RT

更新日期：1984-03-01 00:00:00

abstract：:The capacity for rapid localization of epitope-tagged or fluorescent fusion proteins in cells is an important tool for biological discovery and functional analysis. For Trypanosoma cruzi, the protozoan parasite that causes human Chagas disease, visualization of ectopically-expressed proteins in the clinically-relevant...

journal_title：Molecular and biochemical parasitology

authors： Padmanabhan PK,Polidoro RB,Barteneva NS,Gazzinelli RT,Burleigh BA

更新日期：2014-12-01 00:00:00

abstract：:A restriction fragment from a cloned kinetoplast minicircle DNA has been shown to be diagnostic for Leishmania major. This 402-bp TaqI fragment has been used routinely (as a radiolabelled probe) to detect 10(4) parasites in simple dot blots, both experimentally and in epidemiological surveys. It positively identified ...

journal_title：Molecular and biochemical parasitology

authors： Smith DF,Searle S,Ready PD,Gramiccia M,Ben-Ismail R

更新日期：1989-12-01 00:00:00

abstract：:The body's defense against schistosome infection can take many forms. For example, upon developing acute schistosomiasis, patients often have fever coinciding with larval maturation, migration and early oviposition. As the infection becomes established, the parasite comes under oxidative stress generated by the host i...

journal_title：Molecular and biochemical parasitology

authors： Aragon AD,Imani RA,Blackburn VR,Cunningham C

更新日期：2008-12-01 00:00:00

abstract：:Trypanosome alternative oxidase (TAO) is the cyanide-resistant but SHAM-sensitive terminal oxidase of the mitochondrial electron transport chain in African trypanosomes. The bloodstream forms of Trypanosoma brucei lack cytochromes and respire exclusively via TAO. On the other hand, the insect, or procyclic form posses...

journal_title：Molecular and biochemical parasitology

authors： Walker R Jr,Saha L,Hill GC,Chaudhuri M

更新日期：2005-02-01 00:00:00

abstract：:The secretory organelles of Toxoplasma gondii orchestrate invasion of the host cell and establish the parasitophorous vacuole. Although much has been learned about the roles played by these organelles in invasion by the tachyzoite stage, little is known about the contents or functions of these organelles during bradyz...

journal_title：Molecular and biochemical parasitology

authors： Schwarz JA,Fouts AE,Cummings CA,Ferguson DJ,Boothroyd JC

更新日期：2005-12-01 00:00:00

abstract：:Adenylosuccinate synthetase (AdSS) catalyses the Mg(2+) dependent formation of adenylosuccinate from IMP and aspartate, the reaction being driven by the hydrolysis of GTP to GDP. All characterized AdSS thus far exhibit a random kinetic mechanism. We present here kinetic evidence that unlike all other AdSS, Plasmodium ...

journal_title：Molecular and biochemical parasitology

authors： Raman J,Mehrotra S,Anand RP,Balaram H

更新日期：2004-11-01 00:00:00

abstract：:The cyst wall of Entamoeba invadens (Ei), a model for the human pathogen Entamoeba histolytica, contains chitin, which is a homopolymer of beta-1, 4-linked N-acetyl-glucosamine (GlcNAc). In fungi and in bacteria that make nodulation factors, chitin deacetylases make chitosan, which is a mixture of GlcNAc and glucosami...

journal_title：Molecular and biochemical parasitology

authors： Das S,Van Dellen K,Bulik D,Magnelli P,Cui J,Head J,Robbins PW,Samuelson J

更新日期：2006-07-01 00:00:00

abstract：:Cytoadherence of infected erythrocytes is a hallmark of Plasmodium falciparum infection and a key determinant in the particular virulence of this species. Infected erythrocytes bind a variety of host receptors but certain adhesion traits are associated with more severe disease. A large, diverse protein family named P....

journal_title：Molecular and biochemical parasitology

authors： Kraemer SM,Gupta L,Smith JD

更新日期：2003-06-01 00:00:00

abstract：:The complete sequence of the gene coding for the S antigen from the Wellcome (West African) strain of Plasmodium falciparum has been obtained. It contains a central repetitive region consisting of 65 copies of a partially degenerate 24 bp sequence, coding for a conserved 8 amino acid repeat (Gly Pro Asn Ser Asp Gly As...

journal_title：Molecular and biochemical parasitology

authors： Nicholls SC,Hillman Y,Lockyer MJ,Odink KG,Holder AA

更新日期：1988-02-01 00:00:00

abstract：:A Trypanosoma cruzi small subunit ribosomal RNA gene was sequenced from genomic recombinant plasmid clones. The assigned coding region was 2319 bp, the longest SSU rRNA gene described to date. On the basis of comparisons with published sequences from Crithidia fasciculata, Trypanosoma brucei, and Leishmania donovani, ...

journal_title：Molecular and biochemical parasitology

authors： Hernández R,Rios P,Valdés AM,Piñero D

更新日期：1990-06-01 00:00:00

abstract：:The obligate intracellular protozoan parasite Toxoplasma gondii has a single tubular mitochondrion. During infection, it recruits the host cell's mitochondria abutting to the intracellular vacuole, that contains the parasites. The respective contribution of host and parasitic mitochondria in the intracellular growth o...

journal_title：Molecular and biochemical parasitology

authors： Toursel C,Dzierszinski F,Bernigaud A,Mortuaire M,Tomavo S

更新日期：2000-12-01 00:00:00