Unique kinetic mechanism of Plasmodium falciparum adenylosuccinate synthetase.

abstract：:Trypanosoma brucei survives in mammals by antigenic variation of its surface coat consisting of variant surface glycoprotein (VSG). Trypanosomes change coat mainly by replacing the transcribed VSG genes in an active telomeric expression site by a different VSG gene. There are about 20 different expression sites and tr...

journal_title：Molecular and biochemical parasitology

authors： Borst P,Ulbert S

更新日期：2001-04-25 00:00:00

abstract：:To investigate whether the lysosomes of Trypanosoma brucei are capable of uptake of macromolecules after internalization by the cell, we used Triton WR-1339, a non-digestible macromolecular compound, which is known to cause a marked decrease in the density of hepatic lysosomes due to massive intralysosomal storage. In...

journal_title：Molecular and biochemical parasitology

authors： Opperdoes FR,Van Roy J

更新日期：1982-09-01 00:00:00

abstract：:In the mosquito, Plasmodium sporozoites rupture from oocysts found on the midgut wall, circulate in the hemolymph and invade salivary glands where they wait to be injected into a vertebrate host during a bloodmeal. The mechanisms by which sporozoites specifically attach to and invade salivary glands are not known but ...

journal_title：Molecular and biochemical parasitology

authors： Sidjanski SP,Vanderberg JP,Sinnis P

更新日期：1997-12-01 00:00:00

abstract：:Trypanosoma cruzi attaches and invades a large variety of mammalian cells by receptor-mediated interactions, one of them involving the binding of parasite trans-sialidase to host sialyl receptors. Three proteoglycan-deficient mutants of Chinese hamster ovary (CHO) cells were used to probe the role of host heparin and ...

journal_title：Molecular and biochemical parasitology

authors： Herrera EM,Ming M,Ortega-Barria E,Pereira ME

更新日期：1994-05-01 00:00:00

abstract：:Two NAD(H)-dependent malate dehydrogenase (MDH) isoenzymes were detected in Phytomonas isolated from the lactiferous tubes of Euphorbia characias. The total specific activity in crude extracts using oxaloacetate as substrate was 3.3 U mg-1 of protein. The two isoenzymes had isoelectric points of 6.0 and 7.2, respectiv...

journal_title：Molecular and biochemical parasitology

authors： Uttaro AD,Opperdoes FR

更新日期：1997-10-01 00:00:00

abstract：:The merozoite surface protein-1 of the human malaria parasite Plasmodium falciparum undergoes an extracellular proteolytic cleavage (secondary processing) intrinsic to successful erythrocyte invasion. In the T9/96 clone of P. falciparum the protease responsible has been characterised as a membrane-associated, calcium-...

journal_title：Molecular and biochemical parasitology

authors： Blackman MJ,Chappel JA,Shai S,Holder AA

更新日期：1993-11-01 00:00:00

abstract：:We have identified a GM2-activator protein (GM2AP) with highly unusual properties secreted by the nematode parasite Trichinella spiralis. Expression in Pichia pastoris resulted in a hyperglycosylated protein of 28 kDa, but the 18 kDa native protein was not glycosylated. The parasite GM2AP does not facilitate degradati...

journal_title：Molecular and biochemical parasitology

authors： Bruce AF,Gares MP,Selkirk ME,Gounaris K

更新日期：2006-06-01 00:00:00

abstract：:Sequestration of Plasmodium falciparum infected erythrocytes in the cerebral circulation is strongly implicated in the pathogenesis of cerebral malaria. From previous studies it was postulated that genes essential for cytoadherence were located on the right arm of chromosome 9 as P. falciparum isolates with a deletion...

journal_title：Molecular and biochemical parasitology

authors： Chaiyaroj SC,Coppel RL,Magowan C,Brown GV

更新日期：1994-09-01 00:00:00

abstract：:Hookworm disease is a debilitating worm infection that affects hundreds of millions of people. Despite the existence of anthelmintic drugs, reports have testified of a decrease in efficacy of these drugs. Therefore, it is imperative to find new drugs and drug targets for hookworm disease treatment. In this study we id...

journal_title：Molecular and biochemical parasitology

authors： Rigouin C,Vermeire JJ,Nylin E,Williams DL

更新日期：2013-09-01 00:00:00

abstract：:Genetic diversity within and between tandemly arrayed copies of the Gp63 gene occurs in laboratory isolates of Leishmania spp., but the extent to which this represents natural genetic diversity has not been assessed. Here, the Gp63 locus is examined in 58 fresh isolates of L. peruviana, and clones derived from them, c...

journal_title：Molecular and biochemical parasitology

authors： Espinoza JR,Skinner AC,Davies CR,Llanos-Cuentas A,Arevalo J,Dye C,McMaster WR,Ajioka JW,Blackwell JM

更新日期：1995-06-01 00:00:00

abstract：:Antibodies were affinity purified from crude antiserum by elution from the 24 kDa region of preparative one-dimensional Western blots containing immobilized adult Schistosoma mansoni inner bilayer membrane proteins. They were shown to be specific for a single acidic polypeptide complex, Smgp24, following immunoblottin...

journal_title：Molecular and biochemical parasitology

authors： Karcz SR,Barnard BJ,Podesta RB

更新日期：1988-11-01 00:00:00

abstract：:Malaria parasites possess a de novo heme synthetic pathway. Interestingly, this pathway is dispensable during the blood stages of development in mammalian hosts. The assembly of the two most important hemeproteins, cytochromes c and c1, is mediated by cytochrome heme lyase enzymes. Plasmodium spp. possess two cytochro...

journal_title：Molecular and biochemical parasitology

authors： Posayapisit N,Songsungthong W,Koonyosying P,Falade MO,Uthaipibull C,Yuthavong Y,Shaw PJ,Kamchonwongpaisan S

更新日期：2016-01-01 00:00:00

abstract：:A 25-kb CpFAS1 gene from Cryptosporidium parvum has been engineered and expressed as five individual maltose-binding protein (MBP)-fusion proteins: an N-terminal loading unit, three fatty acyl elongation modules, and a C-terminal reductase. Enzymatic activities of all domains (except the reductase) were individually a...

journal_title：Molecular and biochemical parasitology

authors： Zhu G,Li Y,Cai X,Millership JJ,Marchewka MJ,Keithly JS

更新日期：2004-03-01 00:00:00

abstract：:Tubulin-binding cofactor C stimulates GTPase activity and contributes to the release of the heterodimeric α/β-tubulin from a super-complex of tubulin monomers and two ancillary cofactors. We have determined the 2.2 Å resolution crystal structure of the C-terminal domain of tubulin-binding cofactor C from Leishmania ma...

journal_title：Molecular and biochemical parasitology

authors： Barrack KL,Fyfe PK,Finney AJ,Hunter WN

更新日期：2015-05-01 00:00:00

abstract：:A Trypanosoma cruzi small subunit ribosomal RNA gene was sequenced from genomic recombinant plasmid clones. The assigned coding region was 2319 bp, the longest SSU rRNA gene described to date. On the basis of comparisons with published sequences from Crithidia fasciculata, Trypanosoma brucei, and Leishmania donovani, ...

journal_title：Molecular and biochemical parasitology

authors： Hernández R,Rios P,Valdés AM,Piñero D

更新日期：1990-06-01 00:00:00

abstract：:During intracellular development of the malarial parasite numerous membranous vesicles appear in the infected erythrocyte cytoplasm between the parasitophorous vacuolar membrane (PVM) and the erythrocyte plasma membrane. In this study we describe the characterization of a monoclonal antibody which recognizes two major...

journal_title：Molecular and biochemical parasitology

authors： Martinez SL,Clavijo CA,Winograd E

更新日期：1998-03-15 00:00:00

abstract：:Genetic variation among malaria parasites has important consequences with regard to drug resistance, pathogenicity, immunity, transmission, and speciation. In this regard, malaria parasites have been shown to display a high degree of inter- and intra-species genetic divergence. The nuclear genomes of Plasmodium falcip...

journal_title：Molecular and biochemical parasitology

authors： McIntosh MT,Srivastava R,Vaidya AB

更新日期：1998-09-01 00:00:00

abstract：:A group of Leishmania mexicana cysteine proteases that differ from those previously found in this protozoon are described. The enzymes characteristically have a preference for peptidyl substrates with a phenylalanyl-valyl-arginyl moiety, do not hydrolyse gelatin in substrate-sodium dodecyl sulphate polyacrylamide gels...

journal_title：Molecular and biochemical parasitology

authors： Robertson CD,Coombs GH

更新日期：1993-12-01 00:00:00

abstract：:Cryptosporidium spp. are intracellular apicomplexan parasites that cause outbreaks of waterborne diarrheal disease worldwide. Previous studies had identified a Cryptosporidium parvum sporozoite antigen, CpMuc4, that appeared to be involved in attachment and invasion of the parasite into intestinal epithelial cells. Cp...

journal_title：Molecular and biochemical parasitology

authors： Paluszynski J,Monahan Z,Williams M,Lai O,Morris C,Burns P,O'Connor R

更新日期：2014-02-01 00:00:00

abstract：:The schizont stage of the protozoan parasite Theileria annulata reversibly transforms bovine monocytes into an immortalised and metastatic state. We have been studying T. annulata induction of host matrix metalloproteinases (MMP) which are involved in parasite dissemination and pathogenesis. We have observed that prol...

journal_title：Molecular and biochemical parasitology

authors： Adamson R,Logan M,Kinnaird J,Langsley G,Hall R

更新日期：2000-02-25 00:00:00

abstract：:A novel cDNA was isolated from a library of Cysticercus cellulosae by immunological screening. Sequence analysis reveals that it has typical annexin structures. However, since its N-terminus is distinct and the core domain only has 32-44% amino acid identity to the tetrads of other annexins, the cDNA encodes a member ...

journal_title：Molecular and biochemical parasitology

authors： Hongli Y,Shuhan S,Ruiwen C,Yingjun G

更新日期：2002-01-01 00:00:00

abstract：:Apicomplexa parasites use complex cell cycles to replicate that are not well understood mechanistically. We have established a robust forward genetic strategy to identify the essential components of parasite cell division. Here we describe a novel temperature sensitive Toxoplasma strain, mutant 13-20C2, which growth a...

journal_title：Molecular and biochemical parasitology

authors： Suvorova ES,Lehmann MM,Kratzer S,White MW

更新日期：2012-01-01 00:00:00

abstract：:Malarial merozoites use an array of ligands, including members of the Reticulocyte Binding Like (RBL) super-family of invasion proteins, to identify and invade erythrocytes. RBL family members are large Type I membrane anchored proteins expressed at the invasive end of merozoites that share homology with the Reticuloc...

journal_title：Molecular and biochemical parasitology

authors： Rayner JC,Huber CS,Galinski MR,Barnwell JW

更新日期：2004-02-01 00:00:00

abstract：:Distinct 3'- and 5'-nucleotidase activities were localized to the surface membrane of Leishmania donovani promastigotes using enzymatic reactions with live intact cells and fine structure enzyme-mediated cytochemical reactions with intact cells, cell homogenates, and isolated surface membranes. The results indicated t...

journal_title：Molecular and biochemical parasitology

authors： Dwyer DM,Gottlieb M

更新日期：1984-02-01 00:00:00

abstract：:Per-ARNT-Sim (PAS) domains of proteins play important roles as modules for signalling and cellular regulation processes in widely diverse organisms such as Archaea, Bacteria, protists, plants, yeasts, insects and vertebrates. These domains are present in many proteins where they are used as sensors of stimuli and modu...

journal_title：Molecular and biochemical parasitology

authors： Rojas-Pirela M,Rigden DJ,Michels PA,Cáceres AJ,Concepción JL,Quiñones W

更新日期：2018-01-01 00:00:00

abstract：:The 5' and 3' regions adjacent to the initiation codon in 22 Plasmodium falciparum sequences were examined. A 5' consensus sequence (AAAA/ATG) was found. Although P. falciparum non-translated DNA is A-rich, A occurred significantly more frequently in the 4 positions preceding the initiation ATG than in adjacent non-tr...

journal_title：Molecular and biochemical parasitology

authors： Saul A,Battistutta D

更新日期：1990-08-01 00:00:00

abstract：:Unequal usage of synonymous codons in the gene transcript for an amino acid is known as codon usage bias (CUB). It is a unique property of gene as well as genome. Mutation and natural selection are known to be the major factors that influence CUB. Other factors encompass gene expression level, GC content, codon positi...

journal_title：Molecular and biochemical parasitology

authors： Mazumder GA,Uddin A,Chakraborty S

更新日期：2018-10-01 00:00:00

abstract：:The body's defense against schistosome infection can take many forms. For example, upon developing acute schistosomiasis, patients often have fever coinciding with larval maturation, migration and early oviposition. As the infection becomes established, the parasite comes under oxidative stress generated by the host i...

journal_title：Molecular and biochemical parasitology

authors： Aragon AD,Imani RA,Blackburn VR,Cunningham C

更新日期：2008-12-01 00:00:00

abstract：:Malaria parasites export 'a secretome' of hundreds of proteins, including major virulence determinants, from their endoplasmic reticulum (ER), past the parasite plasma and vacuolar membranes to the host erythrocyte. The export mechanism is high affinity (nanomolar) binding of a host (cell) targeting (HT) motif RxLxE/D...

journal_title：Molecular and biochemical parasitology

authors： Bhattacharjee S,Speicher KD,Stahelin RV,Speicher DW,Haldar K

更新日期：2012-10-01 00:00:00

abstract：:Protein kinase activities in bloodstream and procyclic forms of Trypanosoma brucei have been partially purified and characterised. Cytosolic extracts were separated on DEAE-cellulose and assayed for the ability to phosphorylate histone in the presence of Ca2+ and diacylglycerol. Five peaks of activity were identified ...

journal_title：Molecular and biochemical parasitology

authors： Keith K,Hide G,Tait A

更新日期：1990-11-01 00:00:00