In vivo 31P NMR spectrum of Hymenolepis diminuta and its change on short-term exposure to mebendazole.


:The 31P NMR spectrum of the adult tapeworm, Hymenolepis diminuta, at 37 degrees C during perfusion with physiological saline was composed of 10 peaks. Based on chemical shifts and analysis of worm extracts, the phosphorus components included glucose-6-phosphate, fructose-6-phosphate, phosphorylcholine, glycerophosphoryl choline and -ethanolamine, nucleotide monophosphate-diphosphate and -triphosphate, nicotinamide adenine dinucleotide and uridine diphosphate glucose. The mean level of nucleotide triphosphate was 0.86 nmol (mg fresh weight)-1 and the nucleotide triphosphate/-diphosphate ratio 3.9. Based on the nucleotide triphosphate level, worms were viable for at least 3 h and the intracellular pH was maintained constant at approximately 6.7. Short-term exposure to mebendazole perfused at 11 or 27 microM solubilized in physiological saline containing 0.5% Tween 80 or 0.1% dimethyl sulphoxide had little effect on the nucleotide triphosphate level. Some cytological changes, however, were evident following perfusion of mebendazole. In contrast, exposure to 2,4-dinitrophenol caused a rapid decline in nucleotide triphosphate level. It was concluded that mebendazole does not exert its primary effect on oxidative phosphorylation.


Mol Biochem Parasitol


Thompson SN,Platzer EG,Lee RW




Has Abstract


1987-01-02 00:00:00














  • Trichomonas vaginalis ribosomal RNA: identification and characterisation of the transcription promoter and terminator sequences.

    abstract::Trichomonas vaginalis is a parasitic protozoan of both medical and biological relevance. Transcriptional studies in this organism have focused mainly on type II pol promoters, whereas the elements necessary for transcription by polI or polIII have not been investigated. Here, with the aid of a transient transcription ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Franco B,Hernández R,López-Villaseñor I

    更新日期:2012-09-01 00:00:00

  • A change in the premitotic period of the cell cycle is associated with bradyzoite differentiation in Toxoplasma gondii.

    abstract::We have demonstrated that bradyzoites return to the tissue-cyst stage by a developmental pathway that is indistinguishable from that initiated by sporozoites. Mature bradyzoites, like sporozoites from oocysts, were non-proliferative as they contained uniform 1N DNA contents, and replication occurred only in parasites ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Radke JR,Guerini MN,Jerome M,White MW

    更新日期:2003-10-01 00:00:00

  • Isolation of a female-specific, highly repeated Schistosoma mansoni DNA probe and its use in an assay of cercarial sex.

    abstract::A 476 bp fragment of female-specific Schistosoma mansoni genomic DNA, clone W1, represents a degenerative repeat present in more than 500 copies per female genome, and may be part of the constitutive heterochromatin of the W chromosome. The cloning method described can be used as a general approach for isolating sex-s...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Webster P,Mansour TE,Bieber D

    更新日期:1989-10-01 00:00:00

  • Identification of trypanosomatid PEX19: functional characterization reveals impact on cell growth and glycosome size and number.

    abstract::Glycosomes are peroxisome-like organelles present in trypanosomatid pathogens. These organelles compartmentalize glycolysis, among other reactions, and are essential in both bloodstream and procyclic form Trypanosoma brucei. Peroxins (PEXs) are proteins necessary for biogenesis of peroxisomes and glycosomes. In each a...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Banerjee SK,Kessler PS,Saveria T,Parsons M

    更新日期:2005-07-01 00:00:00

  • Molecular cloning, organellar targeting and developmental expression of mitochondrial chaperone HSP60 in Toxoplasma gondii.

    abstract::The obligate intracellular protozoan parasite Toxoplasma gondii has a single tubular mitochondrion. During infection, it recruits the host cell's mitochondria abutting to the intracellular vacuole, that contains the parasites. The respective contribution of host and parasitic mitochondria in the intracellular growth o...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Toursel C,Dzierszinski F,Bernigaud A,Mortuaire M,Tomavo S

    更新日期:2000-12-01 00:00:00

  • Differentiation of Trypanosoma brucei bloodstream trypomastigotes from long slender to short stumpy-like forms in axenic culture.

    abstract::An axenic cultivation system was used to study the differentiation of Trypanosoma brucei bloodstream forms from long slender to short stumpy-like forms. Trypanosomes in the logarithmic phase are similar to long slender bloodstream forms freshly isolated from infected mice, differing only in the rate of oxygen uptake. ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hamm B,Schindler A,Mecke D,Duszenko M

    更新日期:1990-04-01 00:00:00

  • Structural studies on Plasmodium vivax merozoite surface protein-1.

    abstract::Plasmodium vivax infection is the second most common cause of malaria throughout the world. Like other Plasmodium species, P. vivax has a large protein complex, MSP-1, located on the merozoite surface. The C-terminal MSP-1 sub-unit, MSP-1(42), is cleaved during red blood cell invasion, causing the majority of the comp...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Babon JJ,Morgan WD,Kelly G,Eccleston JF,Feeney J,Holder AA

    更新日期:2007-05-01 00:00:00

  • An S antigen gene from Plasmodium falciparum contains a novel repetitive sequence.

    abstract::The complete sequence of the gene coding for the S antigen from the Wellcome (West African) strain of Plasmodium falciparum has been obtained. It contains a central repetitive region consisting of 65 copies of a partially degenerate 24 bp sequence, coding for a conserved 8 amino acid repeat (Gly Pro Asn Ser Asp Gly As...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Nicholls SC,Hillman Y,Lockyer MJ,Odink KG,Holder AA

    更新日期:1988-02-01 00:00:00

  • Characterisation of a simple, highly repetitive DNA sequence from the parasite Leishmania donovani.

    abstract::Repetitive DNA sequences of the Leishmania donovani genome have been identified by screening a recombinant DNA library made by cloning sheared genomic DNA into the vector pAT153. Bacterial clones containing a highly repetitive DNA sequence have been isolated. DNA sequencing has shown that this sequence is composed of ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ellis J,Crampton J

    更新日期:1988-05-01 00:00:00

  • Control of gene expression in Plasmodium falciparum.

    abstract::Transfection has facilitated a functional analysis of transcriptional processes in the human malarial parasite Plasmodium falciparum, providing the first fascinating glimpses into the mechanisms regulating parasite development and pathogenicity. Here we review our rapidly evolving knowledge of what constitutes a promo...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Horrocks P,Dechering K,Lanzer M

    更新日期:1998-09-15 00:00:00

  • Optical map of the genotype A1 WB C6 Giardia lamblia genome isolate.

    abstract::The Giardia lamblia genome consists of 12 Mb divided among 5 chromosomes ranging in size from approximately 1 to 4 Mb. The assembled contigs of the genotype A1 isolate, WB, were previously mapped along the 5 chromosomes on the basis of hybridization of plasmid clones representing the contigs to chromosomes separated b...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Perry DA,Morrison HG,Adam RD

    更新日期:2011-12-01 00:00:00

  • Expression of a stage-specific lipophosphoglycan in Leishmania major amastigotes.

    abstract::Amastigotes of Leishmania major were isolated from infected mice and radiolabeled for 2 h with [3H]galactose. An acidic [3H]glycoconjugate was extracted from a dilipidated residue fraction with the solvent water/ethanol/diethylether/pyridine/NH4OH (15:15:5:1:0.017). The radioactivity labeled glycoconjugate was found t...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Turco SJ,Sacks DL

    更新日期:1991-03-01 00:00:00

  • An evaluation of different methods for labelling the surface of the filarial nematode Brugia pahangi with 125iodine.

    abstract::The specificity of a range of 125I labelling techniques (Chloramine T, Iodogen, Bolton and Hunter reagent, lactoperoxidase and iodosulfanilic acid) to the surface of the filarial nematode Brugia pahangi was evaluated by autoradiography of sections of labelled worms and of dried SDS-polyacrylamide gels following electr...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Marshall E,Howells RE

    更新日期:1985-06-01 00:00:00

  • Pyrazolopyrimidine metabolism in African trypanosomes: metabolic similarities to Trypanosoma cruzi and Leishmania spp.

    abstract::Growth inhibition and radioisotope incorporation studies with allopurinol (4-hydroxypyrazolo(3,4-d)pyrimidine) have shown that the African trypanosomes are biologically and biochemically similar to Leishmania spp. and Trypanosoma cruzi with respect to their response to this compound. These organisms, as a group, share...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Berens RL,Marr JJ,Brun R

    更新日期:1980-04-01 00:00:00

  • Characterization of trafficking pathways and membrane genesis in malaria-infected erythrocytes.

    abstract::The origin of membraneous structures in the cytoplasm of human erythrocytes infected with the malaria parasite, Plasmodium falciparum, was determined by confocal fluorescence imaging microscopy. When infectious merozoites invaded erythrocytes labeled with the fluorescent, lipophilic, non-exchangeable molecules DiIC16 ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Pouvelle B,Gormley JA,Taraschi TF

    更新日期:1994-07-01 00:00:00

  • Proteomic analysis of Schistosoma mansoni egg secretions.

    abstract::Schistosomiasis remains a largely neglected, global health problem. The morbid pathology of the disease stems from the host's inflammatory response to parasite eggs trapped in host tissues. Long term host/parasite survival is dependent upon the successful modulation of the acute pathological response, which is induced...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Cass CL,Johnson JR,Califf LL,Xu T,Hernandez HJ,Stadecker MJ,Yates JR 3rd,Williams DL

    更新日期:2007-10-01 00:00:00

  • Characterization of a SR protein from Trypanosoma brucei with homology to RNA-binding cis-splicing proteins.

    abstract::The protozoan parasite Trypanosoma brucei relies on trans-splicing to process its mRNAs. A novel nuclear serine/arginine (SR)-rich trypanosomal protein (TSR1) was characterized which contains two RNA recognition motifs. The TSR1 protein appears to be homologous to RNA-binding SR proteins of the cis-splicing machinery ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ismaïli N,Pérez-Morga D,Walsh P,Mayeda A,Pays A,Tebabi P,Krainer AR,Pays E

    更新日期:1999-07-30 00:00:00

  • Ribosomal RNA and protein transcripts persist in the cysts of Entamoeba invadens.

    abstract::In most organisms rDNA transcription ceases under conditions of growth stress. However, we have earlier shown that pre-rRNA accumulates during encystation in Entamoeba invadens. We labeled newly-synthesized rRNA during encystation, with [methyl-(3)H] methionine in the presence of chitinase to enable uptake of isotope....

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ojha S,Ahamad J,Bhattacharya A,Bhattacharya S

    更新日期:2014-06-01 00:00:00

  • Population genetic analysis and sub-structuring in Babesia bovis.

    abstract::The tick-borne protozoan parasite, Babesia bovis is one of the causes of bovine babesiosis, an economically important disease of cattle in tropical and sub-tropical countries. Using the recently published genome sequence of the parasite, we developed a panel of eight mini- and micro-satellite markers and used these to...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Simuunza M,Bilgic H,Karagenc T,Syakalima M,Shiels B,Tait A,Weir W

    更新日期:2011-06-01 00:00:00

  • Chemical modification of fructose bisphosphate aldolase from Trypanosoma brucei compared to aldolase from rabbit muscle and Staphylococcus aureus.

    abstract::Chemical modifications of Class I aldolases from Trypanosoma brucei, rabbit muscle and Staphylococcus aureus with carboxypeptidase A, glyceraldehyde 3-phosphate and cysteine-specific reagents revealed the following differences between the three homologous enzymes. Aldolase from S. aureus was not affected by any of the...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Callens M,Opperdoes FR

    更新日期:1991-07-01 00:00:00

  • Differences in substrate specificity and kinetic properties of the recombinant hexokinases HXK1 and HXK2 from Entamoeba histolytica.

    abstract::Entamoeba histolytica is responsible for amoebic colitis and liver abscess in humans. Entamoeba dispar is a closely related, morphologically indistinguishable nonpathogenic species. The hexokinase (ATP:D-hexose 6-phosphotransferase, EC isoenzyme patterns distinguish the pathogenic and nonpathogenic species. B...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kroschewski H,Ortner S,Steipe B,Scheiner O,Wiedermann G,Duchêne M

    更新日期:2000-01-05 00:00:00

  • The fate of ferriprotorphyrin IX in malaria infected erythrocytes in conjunction with the mode of action of antimalarial drugs.

    abstract::The intraerythrocytic malaria parasite digests considerable amounts of its host cell cytosol, which consists mostly of hemoglobin. In order to avert the toxicity of ferriprotorphyrin IX (FP) thus produced, it is generally accepted that FP is polymerized to the non-toxic hemozoin. Investigating the fate of FP in cultur...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Zhang J,Krugliak M,Ginsburg H

    更新日期:1999-03-15 00:00:00

  • DNA sequence of Crithidia fasciculata kinetoplast minicircles.

    abstract::Kinetoplast DNA (kDNA) networks of the insect trypanosomatid Crithidia fasciculata strain CF-C1 contain a nearly homogeneous population of kDNA minicircles as judged by restriction enzyme cleavage analysis. We have determined the entire nucleotide sequence of the major class of minicircles by analyzing M13 phage clone...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Sugisaki H,Ray DS

    更新日期:1987-04-01 00:00:00

  • The domain on the mouse Duffy protein for Plasmodium yoelii binding and invasion to mouse erythrocytes.

    abstract::Erythrocyte invasion by malaria parasites is a multi-step process requiring specific molecular interactions between merozoites and erythrocyte surface receptors. Human Duffy blood group protein is the receptor for Plasmodium vivax merozoite invasion to red blood cells. The cognate parasite ligand for Duffy protein is ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Xu L,Niu J,Chaudhuri A

    更新日期:2006-04-01 00:00:00

  • Characterization and detection of plant trypanosomatids by sequence analysis of the small subunit ribosomal RNA gene.

    abstract::The complete sequences of the genomic small subunit ribosomal RNA gene from two Phytomonas isolates: one associated with palm pathologies (P. cocos FGuiana) and one found in lactiferous plants with no apparent pathology (P. Euphorbe Senegal), were analyzed. Partial sequences from a number of other Phytomonas isolates ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Marché S,Roth C,Philippe H,Dollet M,Baltz T

    更新日期:1995-04-01 00:00:00

  • Trypanosoma brucei MRE11 is non-essential but influences growth, homologous recombination and DNA double-strand break repair.

    abstract::MRE11 is a conserved multi-functional protein that is important for maintaining genomic integrity in yeast and mammalian cells. By database searching, we identified a full-length candidate MRE11 on Trypanosoma brucei chromosome II. We subsequently cloned and sequenced the corresponding gene from the Lister 427 strain....

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Tan KS,Leal ST,Cross GA

    更新日期:2002-11-01 00:00:00

  • Purification and characterization of gamma-glutamylcysteine synthetase from Ascaris suum.

    abstract::We have purified and characterized the Ascaris suum gamma-glutamylcysteine synthetase, the rate-limiting step in the glutathione biosynthesis. The purified enzyme exhibited a specific activity of 18 U (mg protein)-1. Estimation of the molecular mass of the native enzyme by FPLC on Superdex S-200 revealed the presence ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hussein AS,Walter RD

    更新日期:1995-06-01 00:00:00

  • Surface epitope localization and gene structure of a Babesia bovis 44-kilodalton variable merozoite surface antigen.

    abstract::Variation of Babesia bovis merozoite surface antigens occurs among geographic strains of the parasite. In this and a concurrent report, we investigate this variation at the gene and protein level. Using a monoclonal antibody (mAb 23/70.174), B. bovis gene sequences were identified that encoded a surface epitope of a 4...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Jasmer DP,Reduker DW,Hines SA,Perryman LE,McGuire TC

    更新日期:1992-10-01 00:00:00

  • Substrate specificity studies of the cysteine peptidases falcipain-2 and falcipain-3 from Plasmodium falciparum and demonstration of their kininogenase activity.

    abstract::We studied the substrate specificity requirements of recombinant cysteine peptidases from Plasmodium falciparum, falcipain-2 (FP-2) and falcipain-3 (FP-3), using fluorescence resonance energy transfer (FRET) peptides as substrates. Systematic modifications were introduced in the lead sequence Abz-KLRSSKQ-EDDnp (Abz=or...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Cotrin SS,Gouvêa IE,Melo PM,Bagnaresi P,Assis DM,Araújo MS,Juliano MA,Gazarini ML,Rosenthal PJ,Juliano L,Carmona AK

    更新日期:2013-02-01 00:00:00

  • A Plasmodium falciparum isolate with a chromosome 9 deletion expresses a trypsin-resistant cytoadherence molecule.

    abstract::Sequestration of Plasmodium falciparum infected erythrocytes in the cerebral circulation is strongly implicated in the pathogenesis of cerebral malaria. From previous studies it was postulated that genes essential for cytoadherence were located on the right arm of chromosome 9 as P. falciparum isolates with a deletion...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Chaiyaroj SC,Coppel RL,Magowan C,Brown GV

    更新日期:1994-09-01 00:00:00