Characterization and PCR multiplexing of polymorphic microsatellite loci in the whipworm Trichuris arvicolae, parasite of arvicoline rodents and their cross-species utilization in T. muris, parasite of murines.

abstract：:The ability of Leishmania amastigotes to survive within the drastic environmental changes encountered in the phagolysosomes of mammalian macrophages is heavily dependent on the developmental regulation of a variety of genes. The identification of genes that are expressed preferentially in the mammalian stage of the pa...

journal_title：Molecular and biochemical parasitology

authors： Rochette A,McNicoll F,Girard J,Breton M,Leblanc E,Bergeron MG,Papadopoulou B

更新日期：2005-04-01 00:00:00

abstract：:Conserved amongst all eukaryotes is a family of mitochondrial carrier proteins (SLC25A) responsible for the import of various solutes across the inner mitochondrial membrane. We previously reported that the human parasite Trypanosoma brucei possesses 26 SLC25A proteins (TbMCPs) amongst which two, TbMCP11 and TbMCP8, w...

journal_title：Molecular and biochemical parasitology

authors： Gao F,Voncken F,Colasante C

更新日期：2020-05-01 00:00:00

abstract：:The aim of this study was to investigate the occurrence, distribution and immunochemical properties of antibody-defined carbohydrate epitopes in neutral glycolipid fractions of Schistosoma mansoni eggs, cercariae and adults. The amount of extractable, antigenic, neutral glycolipids was lowest in adult worms, increasin...

journal_title：Molecular and biochemical parasitology

authors： Wuhrer M,Dennis RD,Doenhoff MJ,Bickle Q,Lochnit G,Geyer R

更新日期：1999-10-15 00:00:00

abstract：:The gender of cercarial larvae can only be determined using molecular methods. End point PCR methods that amplify repetitive markers on the W chromosome of the female (ZW) parasites have been developed, but sometimes results are ambiguous or incorrect. To more effectively distinguish sexes, and to determine why end po...

journal_title：Molecular and biochemical parasitology

authors： Chevalier FD,Le Clec'h W,Alves de Mattos AC,LoVerde PT,Anderson TJ

更新日期：2016-01-01 00:00:00

abstract：:The activities of carbamoylphosphate synthase, aspartate transcarbamoylase, dihydroorotase, orotate phosphoribosyl transferase and orotidine-5'-phosphate decarboxylase, five of the six enzymes of pyrimidine biosynthesis, have been measured in Crithidia fasciculata, Trypanosoma cruzi, Leishmania major, Trichomonas vagi...

journal_title：Molecular and biochemical parasitology

authors： Hill B,Kilsby J,Rogerson GW,McIntosh RT,Ginger CD

更新日期：1981-02-01 00:00:00

abstract：:Sequestration of Plasmodium falciparum infected erythrocytes in the cerebral circulation is strongly implicated in the pathogenesis of cerebral malaria. From previous studies it was postulated that genes essential for cytoadherence were located on the right arm of chromosome 9 as P. falciparum isolates with a deletion...

journal_title：Molecular and biochemical parasitology

authors： Chaiyaroj SC,Coppel RL,Magowan C,Brown GV

更新日期：1994-09-01 00:00:00

abstract：:The extent of structural conservation of the Plasmodium falciparum sporozoite surface protein gene, STARP, recently characterized in the T9/96 clone, has been analyzed using the polymerase chain reaction. Results from Ivory Coast and Thai clones, field isolates originating from Brazil and Kenya and laboratory-maintain...

journal_title：Molecular and biochemical parasitology

authors： Fidock DA,Sallenave-Sales S,Sherwood JA,Gachihi GS,Ferreira-da-Cruz MF,Thomas AW,Druilhe P

更新日期：1994-10-01 00:00:00

abstract：:Antibodies raised against a Leishmania major recombinant promastigote surface antigen 2 (PSA-2) fragment recognized three major polypeptides of approximate M(r) 96,000, 80,000 and 50,000 in promastigotes of three Israeli isolates of L. major including the cloned line LRC-L137-V121, but detected a different array of po...

journal_title：Molecular and biochemical parasitology

authors： Symons FM,Murray PJ,Ji H,Simpson RJ,Osborn AH,Cappai R,Handman E

更新日期：1994-09-01 00:00:00

abstract：:The anaerobic, parasitic protist, Trichomonas vaginalis, is characterized by the absence of mitochondria and the presence of double membrane bound organelles called hydrogenosomes. Succinyl-coenzyme A synthetase is a hydrogenosomal enzyme which catalyzes the formation of ATP via substrate-level phosphorylation. We hav...

journal_title：Molecular and biochemical parasitology

authors： Lahti CJ,Bradley PJ,Johnson PJ

更新日期：1994-08-01 00:00:00

abstract：:P-glycoprotein gene amplification has been described in several drug-resistant parasitic protozoa. The first P-glycoprotein related gene described in Leishmania was ltpgpA, a gene frequently amplified in arsenite resistant Leishmania. Hybridization experiments indicated that ltpgpA was part of a gene family. In additi...

journal_title：Molecular and biochemical parasitology

authors： Légaré D,Hettema E,Ouellette M

更新日期：1994-11-01 00:00:00

abstract：:Protein kinase activities in bloodstream and procyclic forms of Trypanosoma brucei have been partially purified and characterised. Cytosolic extracts were separated on DEAE-cellulose and assayed for the ability to phosphorylate histone in the presence of Ca2+ and diacylglycerol. Five peaks of activity were identified ...

journal_title：Molecular and biochemical parasitology

authors： Keith K,Hide G,Tait A

更新日期：1990-11-01 00:00:00

abstract：:The scarcity of molecular tools for genetic manipulation is a critical obstacle for functional genomics studies on Trypanosoma cruzi. The current study adapted an inducible site-specific recombination system based on Dimerizable CRE recombinase (DiCRE). Two vectors for stable transfection were created, a first one to ...

journal_title：Molecular and biochemical parasitology

authors： Kangussu-Marcolino MM,Cunha AP,Avila AR,Herman JP,DaRocha WD

更新日期：2014-12-01 00:00:00

abstract：:The apicoplast is a highly specialized organelle that mediates required functions in the growth and replication of apicomplexan parasites. Despite structural conservation of the apicoplast among different parasite genera and species, there are also critical differences in the metabolic requirements of different parasi...

journal_title：Molecular and biochemical parasitology

authors： Caballero MC,Pedroni MJ,Palmer GH,Suarez CE,Davitt C,Lau AO

更新日期：2012-02-01 00:00:00

abstract：:Amoebiasis is spread by the ingestion of dormant Entamoeba histolytica cysts. Intervention of encystation could break the transmission cycle, thereby reducing disease burden. The model system used to study trophozoite to cyst differentiation is Entamoeba invadens. Here we describe an electroporation-based method for s...

journal_title：Molecular and biochemical parasitology

authors： Singh N,Ojha S,Bhattacharya A,Bhattacharya S

更新日期：2012-07-01 00:00:00

abstract：:Purified GP57/51, a Trypanosoma cruzi glycoprotein earlier identified as a major antigen in infected humans, was subjected to N-terminal sequence analysis. Alignment of the first 30 amino acids revealed that its N-terminal region is virtually identical to that reported for a cysteine-proteinase isolated from the Tulah...

journal_title：Molecular and biochemical parasitology

authors： Murta AC,Persechini PM,Padron Tde S,de Souza W,Guimarães JA,Scharfstein J

更新日期：1990-11-01 00:00:00

abstract：:A polyprotein composed of multiple units arranged in direct tandem arrays has been identified in parasitic and free living nematodes. Analysis of previously cloned units from the Dirofilaria immitis polyprotein antigen (DiPA) indicated the units were nearly identical but here we demonstrate that they segregate into tw...

journal_title：Molecular and biochemical parasitology

authors： Poole CB,Hornstra LJ,Benner JS,Fink JR,McReynolds LA

更新日期：1996-11-12 00:00:00

abstract：:[14C]Artemisinin was taken up by Plasmodium falciparum in culture and concentrated in hemozoin. In vitro, hemin and artemisinin were found to undergo a chemical reaction forming two major products which were isolated by high-performance liquid chromatography (HPLC). The m/z values of the two products were 856 and 871....

journal_title：Molecular and biochemical parasitology

authors： Hong YL,Yang YZ,Meshnick SR

更新日期：1994-01-01 00:00:00

abstract：:The principal constituents of the free amino acid pool in Leishmania tropica promastigotes include alanine, glycine, serine, threonine, alpha-aminobutyric acid and ethanolamine. Changes in levels of the pool constituents during starvation, nutrient supplementation and treatment with a metabolic inhibitor (sodium fluor...

journal_title：Molecular and biochemical parasitology

authors： Simon MW,Jayasimhulu K,Mukkada AJ

更新日期：1983-09-01 00:00:00

abstract：:A genomic clone, pTt21, containing DNA apparently transcribed specifically in Trypanosoma cruzi trypomastigotes, was obtained by differentially screening a genomic library with trypomastigote and epimastigote cDNA. This 3444-bp clone contained open reading frames at each end, separated by a 1.8-kb non-coding region. T...

journal_title：Molecular and biochemical parasitology

authors： Takle GB,O'Connor J,Young AJ,Cross GA

更新日期：1992-11-01 00:00:00

abstract：:In trypanosomes and related kinetoplastida a common short 5' leader sequence is encoded by the highly repetitive mini-exon gene and added to all protein coding mRNAs by trans-splicing. To initiate a search for transcription factors associated with transcription of the mini-exon we have used an electrophoretic mobility...

journal_title：Molecular and biochemical parasitology

authors： Koronakis VE,Ross AM,Le Page RW,Hughes C

更新日期：1988-07-01 00:00:00

abstract：:The complete sequence for a major Schistosome mansoni eggshell protein gene has been determined from a genomic DNA fragment. The use of an open reading frame encoding a glycine-rich polypeptide was confirmed by in vitro translation of schistosome mRNA in the presence of [3H]glycine and comparison with the amino acid c...

journal_title：Molecular and biochemical parasitology

authors： Rodrigues V,Chaudhri M,Knight M,Meadows H,Chambers AE,Taylor WR,Kelly C,Simpson AJ

更新日期：1989-01-01 00:00:00

abstract：:Clones which were selected from a Toxoplasma gondii expression library with the immune serum from a T. gondii-infected rabbit, were further screened using milk and intestinal secretions from mice which had been orally infected with T. gondii cysts. The gene products of several clones reacted strongly with milk IgA and...

journal_title：Molecular and biochemical parasitology

authors： Mevelec MN,Chardès T,Mercereau-Puijalon O,Bourguin I,Achbarou A,Dubremetz JF,Bout D

更新日期：1992-12-01 00:00:00

abstract：:A myofibrillar protein extract has been isolated from the muscle of Ascaris suum. Two-dimensional electrophoresis of this extract revealed that the myosin light chain 1 (ALC1) migrates as 3 components with approximate isoelectric points in the range of 5.3-5.6. The most acidic component of ALC1 appeared to be phosphor...

journal_title：Molecular and biochemical parasitology

authors： Srihari T,Wiehrer W,Pette D,Harris BG

更新日期：1981-06-01 00:00:00

abstract：:Malarial merozoites use an array of ligands, including members of the Reticulocyte Binding Like (RBL) super-family of invasion proteins, to identify and invade erythrocytes. RBL family members are large Type I membrane anchored proteins expressed at the invasive end of merozoites that share homology with the Reticuloc...

journal_title：Molecular and biochemical parasitology

authors： Rayner JC,Huber CS,Galinski MR,Barnwell JW

更新日期：2004-02-01 00:00:00

abstract：:Four Trypanosoma cruzi genomic DNA fragments carrying different TcP2 beta genes have been isolated and sequenced. Three of them had a single TcP2 beta gene, while the 3.8-kb-long DNA segment encoding the TcP2 beta-H1.8 locus showed two TcP2 beta genes arranged in tandem. These genes were physically connected by a 428-...

journal_title：Molecular and biochemical parasitology

authors： Vazquez MP,Schijman AG,Levin MJ

更新日期：1994-04-01 00:00:00

abstract：:A wide range of secondary biological functions have been documented for eukaryotic aminoacyl-tRNA synthetases including roles in transcriptional regulation, mitochondrial RNA splicing, cell growth, and chemokine-like activities. The asparaginyl-tRNA synthetase (AsnRS) of the filarial nematode, Brugia malayi, is a high...

journal_title：Molecular and biochemical parasitology

authors： Kron MA,Wang C,Vodanovic-Jankovic S,Howard OM,Kuhn LA

更新日期：2012-09-01 00:00:00

abstract：:The membrane potential and pH gradient over the plasma membrane of the protozoan parasite Trypanosoma brucei were measured with radioactive indicators in combination with the silicone oil centrifugation technique over a range of temperatures. At 37 degrees C a small membrane potential and pH gradient of similar magnit...

journal_title：Molecular and biochemical parasitology

authors： Ter Kuile BH,Wiemer EA,Michels PA,Opperdoes FR

更新日期：1992-10-01 00:00:00

abstract：:Antigenic variation in African trypanosomes proceeds by switching between different variant surface glycoprotein (VSG) molecules, whose extensive epitope differences enable evasion of antibody responses. Each trypanosome has approximately 1000 basic copy VSG genes inside chromosomes and a subset located at telomeres. ...

journal_title：Molecular and biochemical parasitology

authors： Graham VS,Barry JD

更新日期：1996-07-01 00:00:00

abstract：:When the malarial parasite Plasmodium falciparum multiplies in erythrocytes it dramatically increases uptake of essential metabolic precursors (nucleosides, nucleobases and glucose) and export of lactic acid by undefined mechanisms. The first evidence is provided here, by a detailed study in Xenopus laevis oocytes, th...

journal_title：Molecular and biochemical parasitology

authors： Penny JI,Hall ST,Woodrow CJ,Cowan GM,Gero AM,Krishna S

更新日期：1998-05-15 00:00:00

abstract：:The capability of synthesizing fatty acids de novo in the meront stage of the oyster protozoan parasite, Perkinsus marinus, was investigated employing stable-isotope-labeled precursors (1,2 13C-acetate and palmitic-d(31) acid). Fatty acid methyl esters derived from 1,2 13C-acetate and palmitic-d(31) acid were analyzed...

journal_title：Molecular and biochemical parasitology

authors： Chu FL,Lund E,Soudant P,Harvey E

更新日期：2002-02-01 00:00:00