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Conditional removal of selectable markers in Trypanosoma cruzi using a site-specific recombination tool: proof of concept.

Abstract：

:The scarcity of molecular tools for genetic manipulation is a critical obstacle for functional genomics studies on Trypanosoma cruzi. The current study adapted an inducible site-specific recombination system based on Dimerizable CRE recombinase (DiCRE). Two vectors for stable transfection were created, a first one to express inactive portions of DiCRE recombinase, and a second plasmid containing the loxP sites to test DiCRE activity. After integrating both constructs into the T. cruzi genome, it was shown that DiCRE recombinase can be efficiently used to manipulate its genome by allowing the removal of selectable markers thus generating homogeneous populations. The DiCRE recombinase success allows conditional knockout and the removal of selectable markers without prior parasite modification, which also facilitate the transferring of DiCRE recombinase to different T. cruzi strains.

journal_name

Mol Biochem Parasitol

journal_title

Molecular and biochemical parasitology

authors

Kangussu-Marcolino MM,Cunha AP,Avila AR,Herman JP,DaRocha WD

doi

10.1016/j.molbiopara.2015.01.001

subject

Has Abstract

pub_date

2014-12-01 00:00:00

pages

71-4

issue

2

eissn

0166-6851

issn

1872-9428

pii

S0166-6851(15)00002-X

journal_volume

198

pub_type

杂志文章

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