Differences in substrate specificity and kinetic properties of the recombinant hexokinases HXK1 and HXK2 from Entamoeba histolytica.

abstract：:We have investigated the protein and glycoprotein content of Plasmodium falciparum merozoites by metabolically labeling cultures of schizont-stage parasites with [35S]methionine or with [3H]glucosamine followed by incubation in nonradioactive medium to allow the schizonts to mature into merozoites, infect new erythroc...

journal_title：Molecular and biochemical parasitology

authors： Howard RF,Reese RT

更新日期：1984-03-01 00:00:00

abstract：:The exoglycosidase, beta-N-acetyl-D-hexosaminidase was purified 600-fold from the muscle-stage larvae (L1) of Trichinella spiralis. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), the purified enzyme-active fraction contained 4 polypeptides with apparent molecular weights of 100,000, 68,000, 5...

journal_title：Molecular and biochemical parasitology

authors： Rhoads ML

更新日期：1988-10-01 00:00:00

abstract：:Genetic variation among malaria parasites has important consequences with regard to drug resistance, pathogenicity, immunity, transmission, and speciation. In this regard, malaria parasites have been shown to display a high degree of inter- and intra-species genetic divergence. The nuclear genomes of Plasmodium falcip...

journal_title：Molecular and biochemical parasitology

authors： McIntosh MT,Srivastava R,Vaidya AB

更新日期：1998-09-01 00:00:00

abstract：:In trypanosomatids, removal of hydrogen peroxide and other aryl and alkyl peroxides is achieved by the NADPH-dependent trypanothione peroxidase system, whose components are trypanothione reductase (TRYR), trypanothione, tryparedoxin (TRYX) and tryparedoxin peroxidase (TRYP). Here, we report the cloning of a multi-copy...

journal_title：Molecular and biochemical parasitology

authors： Tetaud E,Giroud C,Prescott AR,Parkin DW,Baltz D,Biteau N,Baltz T,Fairlamb AH

更新日期：2001-09-03 00:00:00

abstract：:Monoclonal antibodies designated 8G10/48 and 9E3/48 raised against mature asexual blood stages of Plasmodium falciparum inhibit parasite growth in vitro. Both antibodies bind to an epitope which includes the linear sequence Ser Thr Asn Ser and which is present in a cDNA clone from a P. falciparum expression library. T...

journal_title：Molecular and biochemical parasitology

authors： Epping RJ,Goldstone SD,Ingram LT,Upcroft JA,Ramasamy R,Cooper JA,Bushell GR,Geysen HM

更新日期：1988-02-01 00:00:00

abstract：:Two dimensional electrophoresis has been used to examine protein variation in clones of two Plasmodium falciparum isolates. Variant forms of 12 proteins were detected. Five genetically distinct parasite types were identified in one isolate, and two in the second isolate. Examination of uncloned parasites using this te...

journal_title：Molecular and biochemical parasitology

authors： Fenton B,Walker A,Walliker D

更新日期：1985-08-01 00:00:00

abstract：:Distinct 3'- and 5'-nucleotidase activities were localized to the surface membrane of Leishmania donovani promastigotes using enzymatic reactions with live intact cells and fine structure enzyme-mediated cytochemical reactions with intact cells, cell homogenates, and isolated surface membranes. The results indicated t...

journal_title：Molecular and biochemical parasitology

authors： Dwyer DM,Gottlieb M

更新日期：1984-02-01 00:00:00

abstract：:A method for a 50-60-fold purification of a cysteine proteinase from trophozoites of Entamoeba histolytica using 35-80% ammonium sulphate fractionation, gel chromatography on Sephadex G-75, and preparative isoelectric focusing is described. The enzyme was examined for its proteolytic potencies towards native enzyme su...

journal_title：Molecular and biochemical parasitology

authors： Scholze H,Werries E

更新日期：1986-01-01 00:00:00

abstract：:Kinetoplast DNA (kDNA) networks of the insect trypanosomatid Crithidia fasciculata strain CF-C1 contain a nearly homogeneous population of kDNA minicircles as judged by restriction enzyme cleavage analysis. We have determined the entire nucleotide sequence of the major class of minicircles by analyzing M13 phage clone...

journal_title：Molecular and biochemical parasitology

authors： Sugisaki H,Ray DS

更新日期：1987-04-01 00:00:00

abstract：:The alpha and beta tubulin genes of Leishmania major were cloned and used to study the genomic organization, chromosomal location and transcription of tubulin genes in L. major. The number of beta tubulin isogenes was determined by hybridization of probes representing the 5' and 3' ends of the cloned beta tubulin cDNA...

journal_title：Molecular and biochemical parasitology

authors： Spithill TW,Samaras N

更新日期：1987-05-01 00:00:00

abstract：:As a start to understanding the importance of intracellular proteolysis in the protozoon Leishmania mexicana, the parasite proteasome has been purified and characterised. The L. mexicana proteasome is similar to proteasomes from other eukaryotes. It is soluble, and the 20S form has a mass of around 670 kDa, composed o...

journal_title：Molecular and biochemical parasitology

authors： Robertson CD

更新日期：1999-09-20 00:00:00

abstract：:Seven beta-naphthylamine-linked peptides were tested as substrates for a previously described thiol proteinase of adult Schistosoma mansoni. The enzyme was not active on carbobenoxy-arginyl-arginyl-4-methoxy-2-naphthylamide and carbobenzoxy-alanyl-arginyl-arginyl-4-methoxy-2-naphthylamide. Enzyme activity was maximal ...

journal_title：Molecular and biochemical parasitology

authors： Dresden MH,Rutledge ML,Chappell CL

更新日期：1981-11-01 00:00:00

abstract：:The ADP ribosylation factors (Arfs) are a highly conserved subfamily of the Ras small GTPases with crucial roles in vesicle budding and membrane trafficking. Unlike in other eukaryotes, the orthologue of Arf1 in the host bloodstream form of Trypanosoma brucei is essential for the maintenance of endocytosis. In contras...

journal_title：Molecular and biochemical parasitology

authors： Price HP,Stark M,Smith B,Smith DF

更新日期：2007-10-01 00:00:00

abstract：:The obligate intracellular protozoan parasite Toxoplasma gondii has a single tubular mitochondrion. During infection, it recruits the host cell's mitochondria abutting to the intracellular vacuole, that contains the parasites. The respective contribution of host and parasitic mitochondria in the intracellular growth o...

journal_title：Molecular and biochemical parasitology

authors： Toursel C,Dzierszinski F,Bernigaud A,Mortuaire M,Tomavo S

更新日期：2000-12-01 00:00:00

abstract：:Multigene families are present on the telomeric and sub-telomeric regions of most chromosomes of the malaria parasite, Plasmodium. The largest gene family identified so far is the Plasmodium interspersed repeat (pir) multigene gene family and is shared by Plasmodium vivax, and simian and rodent malaria species. Most p...

journal_title：Molecular and biochemical parasitology

authors： Cunningham D,Lawton J,Jarra W,Preiser P,Langhorne J

更新日期：2010-04-01 00:00:00

abstract：:Sulfated glycosphingolipids are present on the surface of a variety of cells. They are active participants in adhesion processes in many systems and appear to be involved in the regulation of cell proliferation, differentiation and other developmental cellular events. However, the body of knowledge about synthesis, st...

journal_title：Molecular and biochemical parasitology

authors： Landoni M,Duschak VG,Peres VJ,Nonami H,Erra-Balsells R,Katzin AM,Couto AS

更新日期：2007-07-01 00:00:00

abstract：:Antigenic variation in African trypanosomes proceeds by switching between different variant surface glycoprotein (VSG) molecules, whose extensive epitope differences enable evasion of antibody responses. Each trypanosome has approximately 1000 basic copy VSG genes inside chromosomes and a subset located at telomeres. ...

journal_title：Molecular and biochemical parasitology

authors： Graham VS,Barry JD

更新日期：1996-07-01 00:00:00

abstract：:Adult Schistosoma mansoni worms rapidly degrade their endogenous glycogen stores immediately after isolation from the host. In NCTC 109 or in a diphasic culture medium the glycogen levels slowly recovered again after the initial decrease. The rapid degradation of glycogen could be prevented, even in a simple salt medi...

journal_title：Molecular and biochemical parasitology

authors： Tielens AG,van den Bergh SG

更新日期：1987-07-01 00:00:00

abstract：:Beta 1,3-glucan recognition proteins (GRP) have specific affinity for beta 1,3-glucan, a component on the surface of fungi and bacteria. By interacting with beta 1,3-glucan, GRP initiates activation of prophenoloxidase, a key enzyme in the signaling pathway leading to melanotic encapsulation in invertebrates. In this ...

journal_title：Molecular and biochemical parasitology

authors： Wang X,Fuchs JF,Infanger LC,Rocheleau TA,Hillyer JF,Chen CC,Christensen BM

更新日期：2005-01-01 00:00:00

abstract：:We have undertaken the first comparative pilot gene discovery analysis of approximately 25,000 random genomic and expressed sequence tags (ESTs) from three species of Plasmodium, the infectious agent that causes malaria. A total of 5482 genome survey sequences (GSSs) and 5582 ESTs were generated from mung bean nucleas...

journal_title：Molecular and biochemical parasitology

authors： Carlton JM,Muller R,Yowell CA,Fluegge MR,Sturrock KA,Pritt JR,Vargas-Serrato E,Galinski MR,Barnwell JW,Mulder N,Kanapin A,Cawley SE,Hide WA,Dame JB

更新日期：2001-12-01 00:00:00

abstract：:Sequestration of Plasmodium falciparum infected erythrocytes in the cerebral circulation is strongly implicated in the pathogenesis of cerebral malaria. From previous studies it was postulated that genes essential for cytoadherence were located on the right arm of chromosome 9 as P. falciparum isolates with a deletion...

journal_title：Molecular and biochemical parasitology

authors： Chaiyaroj SC,Coppel RL,Magowan C,Brown GV

更新日期：1994-09-01 00:00:00

abstract：:The complex life cycles of many protozoan parasites require the ability to respond to environmental and developmental cues through regulated gene expression. Traditionally, parasitologists have investigated these mechanisms by identifying and characterizing proteins that are necessary for the regulated expression of t...

journal_title：Molecular and biochemical parasitology

authors： Militello KT,Refour P,Comeaux CA,Duraisingh MT

更新日期：2008-02-01 00:00:00

abstract：:Plasmodium falciparum merozoites invade erythrocytes using a range of alternative ligands that includes erythrocyte binding antigenic proteins (EBAs) and reticulocyte binding protein homologues (Rh). Variation in the expression of some of these genes among culture-adapted parasite lines correlates with the use of diff...

journal_title：Molecular and biochemical parasitology

authors： Nery S,Deans AM,Mosobo M,Marsh K,Rowe JA,Conway DJ

更新日期：2006-10-01 00:00:00

abstract：:Three species of blood fluke from the genus Cardicola are known to parasitize and cause disease in Bluefin Tunas--C. forsteri, C. orientalis, and C. opisthorchis. Although initially believed to be separated by geography and host specificity, recent identification of at least two Cardicola spp. concurrently present wit...

journal_title：Molecular and biochemical parasitology

authors： Polinski M,Hamilton DB,Nowak B,Bridle A

更新日期：2013-09-01 00:00:00

abstract：:In Leishmania mexicana two genes were detected coding for different isoforms of the glycolytic enzyme phosphoglycerate kinase. This situation contrasts with that observed in other Trypanosomatidae (Trypanosoma brucei, Trypanosoma congolense, Crithidia fasciculata) analyzed previously, which all contain three different...

journal_title：Molecular and biochemical parasitology

authors： Adjé CA,Opperdoes FR,Michels PA

更新日期：1997-12-01 00:00:00

abstract：:Post-transcriptional regulation is a key feature controlling gene expression in the protozoan parasite Leishmania. The nine-nucleotide paraflagellar rod regulatory element (PRE) in the 3'UTR of Leishmania mexicana PFR2 is both necessary and sufficient for the observed 10-fold higher level of PFR2 mRNA in promastigotes...

journal_title：Molecular and biochemical parasitology

authors： Holzer TR,Mishra KK,LeBowitz JH,Forney JD

更新日期：2008-01-01 00:00:00

abstract：:The steady-state level of the hsp70 mRNAs of Trypanosoma cruzi cultured at different temperatures and growth conditions has been analyzed by Northern blotting. We show that only one size class of hsp70 mRNA, of about 2.2 kb, is transcribed from the hsp70 cluster and that its transcription is constitutive at 28 degrees...

journal_title：Molecular and biochemical parasitology

authors： Requena JM,Jimenez-Ruiz A,Soto M,Assiego R,Santarén JF,Lopez MC,Patarroyo ME,Alonso C

更新日期：1992-07-01 00:00:00

abstract：:A gene-family, named sep, encoding small exported proteins conserved across Plasmodium species has been identified. SEP proteins (13-16 kDa) contain a predicted signal peptide at the NH(2)-terminus, an internal hydrophobic region and a polymorphic, low-complexity region at the carboxy-terminus. One member of the Plasm...

journal_title：Molecular and biochemical parasitology

authors： Birago C,Albanesi V,Silvestrini F,Picci L,Pizzi E,Alano P,Pace T,Ponzi M

更新日期：2003-02-01 00:00:00

abstract：:In trypanosomes, trans-splicing is a major essential RNA-processing mechanism that involves the addition of a spliced leader sequence to all mRNAs from a small RNA species, known as the spliced leader RNA (SL RNA). SL RNA maturation is poorly understood and it is not clear where assembly with Sm proteins takes place. ...

journal_title：Molecular and biochemical parasitology

authors： Biton M,Mandelboim M,Arvatz G,Michaeli S

更新日期：2006-12-01 00:00:00

abstract：:Apicomplexan parasites utilize a peripheral membrane system called the inner membrane complex (IMC) to facilitate host cell invasion and parasite replication. We recently identified a novel family of Toxoplasma IMC Sub-compartment Proteins (ISP1/2/3) that localize to sub-domains of the IMC using a targeting mechanism ...

journal_title：Molecular and biochemical parasitology

authors： Fung C,Beck JR,Robertson SD,Gubbels MJ,Bradley PJ

更新日期：2012-08-01 00:00:00