The Leishmania mexicana proteasome.


:As a start to understanding the importance of intracellular proteolysis in the protozoon Leishmania mexicana, the parasite proteasome has been purified and characterised. The L. mexicana proteasome is similar to proteasomes from other eukaryotes. It is soluble, and the 20S form has a mass of around 670 kDa, composed of at least 10 distinct subunits in the 22 to 32 kDa size range. The molecular mass of the L. mexicana proteasome increases to 1200 kDa in the presence of adenosine-5'-triphosphate, consistent with there being a 26S proteasome in the parasite. The purified 20S proteasome has activity towards substrates with hydrophobic, basic and acidic P, residues, and is sensitive to a range of peptide aldehyde inhibitors, as well as the proteasome-specific inhibitor lactacystin. The peptide aldehydes are able to arrest parasite growth in vitro with the same relative effectiveness as against the purified proteasome activity. The parasite population arrests with an increased 4N DNA content, indicating that, in part, the essential nature of the proteasome for L. mexicana proliferation is due to a role in the parasite cell cycle. Surprisingly, lactacystin is a relatively inefficient inhibitor of L. mexicana growth in vitro.


Mol Biochem Parasitol


Robertson CD




Has Abstract


1999-09-20 00:00:00














  • Arp1, an actin-related protein, in Plasmodium berghei.

    abstract::Actin-related proteins (Arps) constitute a family of eukaryotic cytoskeletal proteins involved in such diverse events as cell motility, cytokinesis, vesicle transport, and chromatin remodelling. Previously, in a study of Plasmodium berghei gene expression in ookinetes and oocysts, we detected stage-specific increased ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Siden-Kiamos I,Schüler H,Liakopoulos D,Louis C

    更新日期:2010-10-01 00:00:00

  • Antigenic variation and cytoadhesion in Babesia bovis and Plasmodium falciparum: different logics achieve the same goal.

    abstract::Babesia bovis is a protozoal hemoparasite of cattle which behaves in certain crucial respects like Plasmodium falciparum, despite being phylogenetically distant and having many differences in its life cycle. The shared behavioral attributes of rapid antigenic variation and cytoadhesion/sequestration are thought to con...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Allred DR,Al-Khedery B

    更新日期:2004-03-01 00:00:00

  • Characterisation of the gene encoding a 104-kilodalton microneme-rhoptry protein of Theileria parva.

    abstract::A neutralizing antiserum, C16, raised against sporozoites of Theileria parva parva was used to screen a lambda gt11 expression library of T. parva parva (Muguga) genomic DNA fragments. Proteins encoded by one phage clone, lambda TpS-17, were reactive with the C16 antiserum. Detailed characterisation of the DNA insert ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Iams KP,Young JR,Nene V,Desai J,Webster P,ole-MoiYoi OK,Musoke AJ

    更新日期:1990-02-01 00:00:00

  • Translation initiation factors GleIF4E2 and GleIF4A can interact directly with the components of the pre-initiation complex to facilitate translation initiation in Giardia lamblia.

    abstract::Translation initiation factor eIF4F is essential for cap-dependent translation initiation in eukaryotes. eIF4F is a trimeric complex consisting of a scaffold protein eIF4G, cap-binding protein eIF4E and DEAD-box RNA helicase eIF4A. eIF4F binds to the 5' cap structure of the mRNA through eIF4E and facilitates the bindi...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Adedoja AN,McMahan T,Neal JP,Hamal Dhakal S,Jois S,Romo D,Hull K,Garlapati S

    更新日期:2020-03-01 00:00:00

  • Some kinetic properties of pyruvate kinase from Trypanosoma brucei: influence of pH and fructose-1,6-diphosphate.

    abstract::The influence of pH on the activity of purified pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC from Trypanosoma brucei has been studied. The Km for the coenzyme ADP is pH-dependent and shows the involvement of a dissociable group on the free enzyme with a pKa of 6.5-6.7. The cooperative interaction...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Flynn IW,Bowman IB

    更新日期:1981-11-01 00:00:00

  • Crystal structure and putative substrate identification for the Entamoeba histolytica low molecular weight tyrosine phosphatase.

    abstract::Entamoeba histolytica is a eukaryotic intestinal parasite of humans, and is endemic in developing countries. We have characterized the E. histolytica putative low molecular weight protein tyrosine phosphatase (LMW-PTP). The structure for this amebic tyrosine phosphatase was solved, showing the ligand-induced conformat...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Linford AS,Jiang NM,Edwards TE,Sherman NE,Van Voorhis WC,Stewart LJ,Myler PJ,Staker BL,Petri WA Jr

    更新日期:2014-01-01 00:00:00

  • The role of cAMP in the actions of the peptide AF3 in the parasitic nematodes Ascaris suum and Ascaridia galli.

    abstract::AF3 (AVPGVLRFamide) is an endogenous RFamide-like peptide isolated from the parasitic nematode Ascaris suum. It has a potent and long lasting excitatory effect in A. suum and Ascaridia galli. This is mediated by a mechanism independent of the nicotinic-like acetylcholine (ACh) receptor, which mediates excitatory trans...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Trim N,Brooman JE,Holden-Dye L,Walker RJ

    更新日期:1998-06-01 00:00:00

  • Characterization of a Fasciola gigantica protein carrying two DM9 domains reveals cellular relocalization property.

    abstract::Even at the present age of whole-organism analysis, e.g., genomics, transcriptomics, and proteomics, the biological roles of many proteins remain unresolved. Classified among the proteins of unknown function is a family of proteins harboring repeats of the DM9 domain, a 60-75 amino acids motif first described in a sma...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Phadungsil W,Smooker PM,Vichasri-Grams S,Grams R

    更新日期:2016-01-01 00:00:00

  • Characterization of tubulin genes in Trypanosoma rangeli.

    abstract::Tubulin genes in Trypanosoma rangeli, the only trypanosome besides T. cruzi to infect humans in America, are organized in homogeneous, alternate alpha and beta gene tandem repeats of 3.8 kb. The basic repeat was cloned, mapped and partially sequenced. In contrast to most other eukaryotes, where tubulin genes are scatt...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Esquenazi D,Morel CM,Traub-Cseko YM

    更新日期:1989-05-15 00:00:00

  • Cathepsin L proteinase secreted by Fasciola hepatica in vitro prevents antibody-mediated eosinophil attachment to newly excysted juveniles.

    abstract::Cathepsin L-like activity was demonstrated in the excretory/secretory (E/S) products of Fasciola hepatica newly excysted juveniles (NEJ), 3-week-old, 5-week-old and mature flukes using the fluorogenic substituted 7-amino-4-methylcoumarin substrates Z-phe-arg-AMC, Z-arg-arg-AMC and Z-arg-AMC. Gelatin-substrate polyacry...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Carmona C,Dowd AJ,Smith AM,Dalton JP

    更新日期:1993-11-01 00:00:00

  • Isolation and partial sequence of a collagen gene from the human filarial parasite Brugia malayi.

    abstract::We report the isolation and sequence of a part of a gene encoding a collagen from the genome of the human filarial parasite Brugia malayi. The deduced amino acid sequence of the sequenced portion of this gene, which we have designated BmCol1, differs from the most catalogued nematode collagens in that it is composed p...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Caulagi VR,Werner C,Rajan TV

    更新日期:1991-03-01 00:00:00

  • A role for tertiary structure in the generation of antigenic diversity and molecular association of the Tams1 polypeptide in Theileria annulata.

    abstract::The major merozoite-piroplasm surface antigen (mMPSA) of Theileria annulata, Tams1, is known to be antigenically diverse. The possession of variable N-linked glycosylation sites and removal of monoclonal antibody 5E1 reactivity by mild periodate treatment suggested, previously, that divergent epitopes may be conferred...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Katzer F,McKellar S,Ferguson MA,d'Oliveira C,Shiels BR

    更新日期:2002-06-01 00:00:00

  • Demonstration of glycosomes (microbodies) in the Bodonid flagellate Trypanoplasma borelli (Protozoa, Kinetoplastida).

    abstract::Homogenates of Trypanoplasma borelli were subjected to subcellular fractionation by sequential differential and isopycnic centrifugation in sucrose. Glycerol-3-phosphate dehydrogenase and the glycolytic enzymes, glucosephosphate isomerase and triosephosphate isomerase, as well as the peroxisomal marker enzyme catalase...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Opperdoes FR,Nohynkova E,Van Schaftingen E,Lambeir AM,Veenhuis M,Van Roy J

    更新日期:1988-08-01 00:00:00

  • Surface membrane localization of 3'- and 5'-nucleotidase activities in Leishmania donovani promastigotes.

    abstract::Distinct 3'- and 5'-nucleotidase activities were localized to the surface membrane of Leishmania donovani promastigotes using enzymatic reactions with live intact cells and fine structure enzyme-mediated cytochemical reactions with intact cells, cell homogenates, and isolated surface membranes. The results indicated t...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Dwyer DM,Gottlieb M

    更新日期:1984-02-01 00:00:00

  • The complexity of the secreted NPA and FAR lipid-binding protein families of Haemonchus contortus revealed by an iterative proteomics-bioinformatics approach.

    abstract::Two different classes of small nematode specific lipid-binding proteins, the nematode polyprotein allergens/antigens (NPAs) and the fatty acid- and retinol-binding (FAR) proteins, are secreted by helminth parasites. Until now, there was no evidence of the expression or secretion of these two families of proteins in Ha...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kuang L,Colgrave ML,Bagnall NH,Knox MR,Qian M,Wijffels G

    更新日期:2009-11-01 00:00:00

  • Reduced purine accumulation is encoded on an amplified DNA in Leishmania mexicana amazonensis resistant to toxic nucleosides.

    abstract::Nucleoside analogs are potential anti-Leishmania agents. To better understand how these compounds might lose their effectiveness, Leishmania were independently selected for resistance to inosine dialdehyde or tubercidin. Each of the resistant cells exhibited resistance to inosine dialdehyde and tubercidin as well as t...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kerby BR,Detke S

    更新日期:1993-08-01 00:00:00

  • The varieties of gene amplification, diversification and hypervariability in the human malaria parasite, Plasmodium falciparum.

    abstract::The human malaria parasite, Plasmodium falciparum, is able to evade host cell-mediated and humoral immunity to maintain both persistent and repeated infections. Immune evasion is in part due to a robust repertoire of proteins which participate in host-parasite interactions but also exhibit profound antigenic diversity...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Templeton TJ

    更新日期:2009-08-01 00:00:00

  • Lipid alterations induced by nifurtimox in Trypanosoma cruzi.

    abstract::The influence of growth in the presence of nifurtimox on total lipids of Trypanosoma cruzi epimastigotes was determined. Nifurtimox-treated organisms showed greater amounts of unsaturated fatty acids as compared with control cells. 18:2 was the major component in the total esterified lipid fraction. Similar results we...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Barreto-Bergter E,Hogge L,Steele da Cruz F

    更新日期:1986-12-01 00:00:00

  • Choline transport in Leishmania major promastigotes and its inhibition by choline and phosphocholine analogs.

    abstract::Phosphatidylcholine is the most abundant phospholipid in the membranes of the human parasite Leishmania. The metabolic pathways leading to its biosynthesis are likely to play a critical role in parasite development and survival and may offer a good target for antileishmanial chemotherapy. Phosphatidylcholine synthesis...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Zufferey R,Mamoun CB

    更新日期:2002-11-01 00:00:00

  • The Kennedy phospholipid biosynthesis pathways are refractory to genetic disruption in Plasmodium berghei and therefore appear essential in blood stages.

    abstract::Phosphatidylcholine (PC) and phosphatidylethanolamine (PE) are the main membrane phospholipids (PLs) of Plasmodium parasites and can be generated by the de novo (Kennedy) CDP-choline and CDP-ethanolamine pathways and by the CDP-diacylglycerol dependent pathway. The Kennedy pathways initiate from exogenous choline and ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Déchamps S,Wengelnik K,Berry-Sterkers L,Cerdan R,Vial HJ,Gannoun-Zaki L

    更新日期:2010-10-01 00:00:00

  • Zinc finger nuclease technology: A stable tool for high efficiency transformation in bloodstream form T. brucei.

    abstract::In Trypanosoma brucei, the generation of knockout mutants is relatively easy compared to other organisms as transfection methods are well established. These methods have their limitations, however, when it comes to the generation of genome-wide libraries that require a minimum of several hundred thousand transformants...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Schumann G,Kangussu-Marcolino MM,Doiron N,Käser S,de Assis Burle-Caldas G,DaRocha WD,Teixeira SM,Roditi I

    更新日期:2017-04-01 00:00:00

  • Involvement of lysosomes in the uptake of macromolecular material by bloodstream forms of Trypanosoma brucei.

    abstract::To investigate whether the lysosomes of Trypanosoma brucei are capable of uptake of macromolecules after internalization by the cell, we used Triton WR-1339, a non-digestible macromolecular compound, which is known to cause a marked decrease in the density of hepatic lysosomes due to massive intralysosomal storage. In...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Opperdoes FR,Van Roy J

    更新日期:1982-09-01 00:00:00

  • Leupeptin alters the proteolytic processing of P126, the major parasitophorous vacuole antigen of Plasmodium falciparum.

    abstract::Among several protease inhibitors tested, only leupeptin was found to modify qualitatively the processing of P126, a major antigen of the parasitophorous vacuole of Plasmodium falciparum, and to inhibit the release of merozoites. Whereas P126 is normally processed upon merozoite release into 2 polypeptides of 50 and 7...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Debrabant A,Delplace P

    更新日期:1989-03-01 00:00:00

  • Differences in the surface radioiodinated proteins of skin and uterine microfilariae of Onchocerca gibsoni.

    abstract::Surface labeling studies using two populations of Onchocerca gibsoni microfilariae revealed important differences in major radioiodinated proteins. Small numbers of microfilariae harvested from the skin of cattle or the uteri of adult worms from skin nodules were purified, radioiodinated, solubilized and the proteins ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Forsyth KP,Copeman DB,Mitchell GF

    更新日期:1984-02-01 00:00:00

  • A diamidine-resistant Trypanosoma equiperdum clone contains a P2 purine transporter with reduced substrate affinity.

    abstract::Following the demonstration that the transport of melaminophenyl arsenical drugs in Trypanosoma brucei is dependent upon an unusual adenosine nucleoside transporter (Carter and Fairlamb, Nature 361 (1993) 173-175) we have investigated adenosine transport in the related parasite Trypanosoma equiperdum (Botat1.1) and a ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Barrett MP,Zhang ZQ,Denise H,Giroud C,Baltz T

    更新日期:1995-07-01 00:00:00

  • Context-dependent roles of the Entamoeba histolytica core promoter element GAAC in transcriptional activation and protein complex assembly.

    abstract::Transcriptional control of the hgl5 gene of Entamoeba histolytica is mediated through an unusual core promoter composed of TATA, GAAC and Initiator elements. In the hgl5 promoter the GAAC element (AATGAACT) determines the site and rate of transcription initiation. Here we tested the role of the GAAC element in transcr...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Singh U,Gilchrist CA,Schaenman JM,Rogers JB,Hockensmith JW,Mann BJ,Petri WA

    更新日期:2002-03-01 00:00:00

  • Identification by extrachromosomal amplification and overexpression of a zeta-crystallin/NADPH-oxidoreductase homologue constitutively expressed in Leishmania spp.

    abstract::A gene which overexpresses a 36-kDa protein (p36) in tunicamycin-resistant Leishmania was mapped by transfection and overexpression to the upstream region of the drug maker in the extrachromosomal amplicon. Complete sequencing of this region revealed a single open reading frame of about 1 kb. Authenticity of the clone...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Liu X,Chang KP

    更新日期:1994-08-01 00:00:00

  • Role of 3'-untranslated regions in the regulation of hexose transporter mRNAs in Trypanosoma brucei.

    abstract::Trypanosoma brucei is a unicellular parasite that is transmitted from one mammalian host to the next by tsetse flies. The expression of many trypanosome genes is regulated during the life cycle but there is no evidence for developmental control of transcription by RNA polymerase II. T. brucei expresses at least two he...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hotz HR,Lorenz P,Fischer R,Krieger S,Clayton C

    更新日期:1995-12-01 00:00:00

  • The fate of ferriprotorphyrin IX in malaria infected erythrocytes in conjunction with the mode of action of antimalarial drugs.

    abstract::The intraerythrocytic malaria parasite digests considerable amounts of its host cell cytosol, which consists mostly of hemoglobin. In order to avert the toxicity of ferriprotorphyrin IX (FP) thus produced, it is generally accepted that FP is polymerized to the non-toxic hemozoin. Investigating the fate of FP in cultur...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Zhang J,Krugliak M,Ginsburg H

    更新日期:1999-03-15 00:00:00

  • Molecular epidemiology of giardiasis.

    abstract::Giardia duodenalis is a widespread parasite of mammalian species, including humans. Due to its invariant morphology, investigation on aspects such as host specificity and transmission patterns requires a direct genetic characterization of cysts/trophozoites from host samples. A number of molecular assays have been dev...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Cacciò SM,Ryan U

    更新日期:2008-08-01 00:00:00