Cloning and analysis of the RESA-2 gene: a DNA homologue of the ring-infected erythrocyte surface antigen gene of Plasmodium falciparum.

abstract：:Malaria parasites export 'a secretome' of hundreds of proteins, including major virulence determinants, from their endoplasmic reticulum (ER), past the parasite plasma and vacuolar membranes to the host erythrocyte. The export mechanism is high affinity (nanomolar) binding of a host (cell) targeting (HT) motif RxLxE/D...

journal_title：Molecular and biochemical parasitology

authors： Bhattacharjee S,Speicher KD,Stahelin RV,Speicher DW,Haldar K

更新日期：2012-10-01 00:00:00

abstract：:In Trypanosoma brucei, the generation of knockout mutants is relatively easy compared to other organisms as transfection methods are well established. These methods have their limitations, however, when it comes to the generation of genome-wide libraries that require a minimum of several hundred thousand transformants...

journal_title：Molecular and biochemical parasitology

authors： Schumann G,Kangussu-Marcolino MM,Doiron N,Käser S,de Assis Burle-Caldas G,DaRocha WD,Teixeira SM,Roditi I

更新日期：2017-04-01 00:00:00

abstract：:The anthelmintic cyclooctadepsipeptide emodepside is effective against nematodes showing resistance against established drug classes. Emodepside exerts its nematicidal effects mainly through its validated target, the tetrameric voltage- and calcium-activated potassium channel SLO-1. Two slo-1 genes were described in T...

journal_title：Molecular and biochemical parasitology

authors： Yilmaz E,Kulke D,von Samson-Himmelstjerna G,Krücken J

更新日期：2015-01-01 00:00:00

abstract：:Several proteins synthesized by mature asexual stages of Plasmodium falciparum interact with the erythrocyte membrane skeleton. One of these is the mature-parasite-infected erythrocyte surface antigen (MESA; also called PfEMP2), a phosphoprotein of 250-300 kDa, which is found on the internal face of the erythrocyte me...

journal_title：Molecular and biochemical parasitology

authors： Lustigman S,Anders RF,Brown GV,Coppel RL

更新日期：1990-01-15 00:00:00

abstract：:Two NAD(H)-dependent malate dehydrogenase (MDH) isoenzymes were detected in Phytomonas isolated from the lactiferous tubes of Euphorbia characias. The total specific activity in crude extracts using oxaloacetate as substrate was 3.3 U mg-1 of protein. The two isoenzymes had isoelectric points of 6.0 and 7.2, respectiv...

journal_title：Molecular and biochemical parasitology

authors： Uttaro AD,Opperdoes FR

更新日期：1997-10-01 00:00:00

abstract：:The effect of 9 monosaccharides which constitute cell surface carbohydrates on the infection of bovine embryonic skin and muscle (BESM) cells by Trypanosoma cruzi trypomastigotes and Toxoplasma gondii tachyzoites was assayed. Most of the monosaccharides tested stimulated the infection of BESM cells by T. gondii; none ...

journal_title：Molecular and biochemical parasitology

authors： Crane MS,Dvorak JA

更新日期：1982-05-01 00:00:00

abstract：:Beta 1,3-glucan recognition proteins (GRP) have specific affinity for beta 1,3-glucan, a component on the surface of fungi and bacteria. By interacting with beta 1,3-glucan, GRP initiates activation of prophenoloxidase, a key enzyme in the signaling pathway leading to melanotic encapsulation in invertebrates. In this ...

journal_title：Molecular and biochemical parasitology

authors： Wang X,Fuchs JF,Infanger LC,Rocheleau TA,Hillyer JF,Chen CC,Christensen BM

更新日期：2005-01-01 00:00:00

abstract：:Laminin is a major constituent of the basal lamina surrounding the midgut of the malaria vectors that has been implicated in the development of the Plasmodium oocyst. In this report we describe the cloning of the Anopheles gambiae gene encoding the laminin gamma 1 polypeptide and follow its expression during mosquito ...

journal_title：Molecular and biochemical parasitology

authors： Vlachou D,Lycett G,Sidén-Kiamos I,Blass C,Sinden RE,Louis C

更新日期：2001-02-01 00:00:00

abstract：:Asexual intraerythrocytic Plasmodium falciparum were shown to have a single isoenzyme of malate dehydrogenase. This malate dehydrogenase was purified to apparent homogeneity using a three-step purification protocol. The parasite malate dehydrogenase had an apparent subunit molecular weight of 32 kDa, a pH optimum of 7...

journal_title：Molecular and biochemical parasitology

authors： Lang-Unnasch N

更新日期：1992-01-01 00:00:00

abstract：:Eimeria are the causative agents of coccidosis, a disease which is of world wide economic importance in the poultry industry. Immunity resulting from infection is species-specific and both antibody and cell-mediated responses have been implicated. As an initial step in the development of a genetically-engineered vacci...

journal_title：Molecular and biochemical parasitology

authors： Clarke LE,Messer LI,Greenwood NM,Wisher MH

更新日期：1987-01-02 00:00:00

abstract：:The complete sequence of the gene coding for the S antigen from the Wellcome (West African) strain of Plasmodium falciparum has been obtained. It contains a central repetitive region consisting of 65 copies of a partially degenerate 24 bp sequence, coding for a conserved 8 amino acid repeat (Gly Pro Asn Ser Asp Gly As...

journal_title：Molecular and biochemical parasitology

authors： Nicholls SC,Hillman Y,Lockyer MJ,Odink KG,Holder AA

更新日期：1988-02-01 00:00:00

abstract：:Contrary to previous reports in the literature, bloodstream forms of the haemoflagellate protozoan Trypanosoma brucei brucei are not deficient in their ability to metabolize hydrogen peroxide, although they either lack or only possess the normal enzymes for H2O2 detoxification, catalase (EC 1.11.1.6) and glutathione p...

journal_title：Molecular and biochemical parasitology

authors： Penketh PG,Klein RA

更新日期：1986-08-01 00:00:00

abstract：:Cryptosporidium spp. are intracellular apicomplexan parasites that cause outbreaks of waterborne diarrheal disease worldwide. Previous studies had identified a Cryptosporidium parvum sporozoite antigen, CpMuc4, that appeared to be involved in attachment and invasion of the parasite into intestinal epithelial cells. Cp...

journal_title：Molecular and biochemical parasitology

authors： Paluszynski J,Monahan Z,Williams M,Lai O,Morris C,Burns P,O'Connor R

更新日期：2014-02-01 00:00:00

abstract：:Plasmepsins (PM), aspartic proteases of Plasmodium, comprises a family of ten proteins that perform critical functions in Plasmodium life cycle. Except VII and VIII, functions of the remaining plasmepsin members have been well characterized. Here, we have generated a mutant parasite lacking PM VII in Plasmodium berghe...

journal_title：Molecular and biochemical parasitology

authors： Mastan BS,Kumari A,Gupta D,Mishra S,Kumar KA

更新日期：2014-06-01 00:00:00

abstract：:AF3 (AVPGVLRFamide) is an endogenous RFamide-like peptide isolated from the parasitic nematode Ascaris suum. It has a potent and long lasting excitatory effect in A. suum and Ascaridia galli. This is mediated by a mechanism independent of the nicotinic-like acetylcholine (ACh) receptor, which mediates excitatory trans...

journal_title：Molecular and biochemical parasitology

authors： Trim N,Brooman JE,Holden-Dye L,Walker RJ

更新日期：1998-06-01 00:00:00

abstract：:As an intracellular parasite, Trypanosoma cruzi is exposed to reactive oxygen species. The study of the proteins involved in the hydroperoxide detoxification cascade, tryparedoxin peroxidase included, may lead to the development of a more specific chemotherapy for Chagas'disease. In this work, the involvement of TcCPX...

journal_title：Molecular and biochemical parasitology

authors： Finzi JK,Chiavegatto CW,Corat KF,Lopez JA,Cabrera OG,Mielniczki-Pereira AA,Colli W,Alves MJ,Gadelha FR

更新日期：2004-01-01 00:00:00

abstract：:The binding of tritiated benzimidazoles (BZs)-albendazole, parbendazole, oxibendazole, mebendazole, oxfendazole and fenbendazole-to crude tubulin extracts from BZ-susceptible and -resistant Haemonchus contortus has been examined. For all BZs, the binding was substantially lower in the resistant isolate. The extent of ...

journal_title：Molecular and biochemical parasitology

authors： Lacey E,Prichard RK

更新日期：1986-05-01 00:00:00

abstract：:A Trypanosoma cruzi small subunit ribosomal RNA gene was sequenced from genomic recombinant plasmid clones. The assigned coding region was 2319 bp, the longest SSU rRNA gene described to date. On the basis of comparisons with published sequences from Crithidia fasciculata, Trypanosoma brucei, and Leishmania donovani, ...

journal_title：Molecular and biochemical parasitology

authors： Hernández R,Rios P,Valdés AM,Piñero D

更新日期：1990-06-01 00:00:00

abstract：:The dsRNA virus which infects some strains of Giardia lamblia has been purified and characterized with respect to its effect on growth of the parasite. Extensive purification of the virus from G. lamblia growth medium was accomplished by Millipore filtration and two successive CsCl gradient centrifugations. The purifi...

journal_title：Molecular and biochemical parasitology

authors： Miller RL,Wang AL,Wang CC

更新日期：1988-04-01 00:00:00

abstract：:The livers of chickens maintained on a diet containing an effective in vivo level (70 ppm) of arprinocid, an anticoccidial agent, were found to contain 0.64 ppm of this drug and 0.33 ppm of a metabolite, arprinocid-1-N-oxide. When arprinocid was tested against Eimeria tenella in cultures of chick kidney epithelial cel...

journal_title：Molecular and biochemical parasitology

authors： Wang CC,Simashkevich PM

更新日期：1980-10-01 00:00:00

abstract：:We have investigated the protein and glycoprotein content of Plasmodium falciparum merozoites by metabolically labeling cultures of schizont-stage parasites with [35S]methionine or with [3H]glucosamine followed by incubation in nonradioactive medium to allow the schizonts to mature into merozoites, infect new erythroc...

journal_title：Molecular and biochemical parasitology

authors： Howard RF,Reese RT

更新日期：1984-03-01 00:00:00

abstract：:This study further explores the effects of hypoxia and acute osmotic stress on intermediary metabolism of Leishmania major and Leishmania donovani. Late log phase promastigotes were washed and incubated with glucose as the sole exogenous carbon source, and rates of glucose consumption and product formation were measur...

journal_title：Molecular and biochemical parasitology

authors： Walsh MJ,Blum JJ

更新日期：1992-02-01 00:00:00

abstract：:Trypanosoma cruzi attaches and invades a large variety of mammalian cells by receptor-mediated interactions, one of them involving the binding of parasite trans-sialidase to host sialyl receptors. Three proteoglycan-deficient mutants of Chinese hamster ovary (CHO) cells were used to probe the role of host heparin and ...

journal_title：Molecular and biochemical parasitology

authors： Herrera EM,Ming M,Ortega-Barria E,Pereira ME

更新日期：1994-05-01 00:00:00

abstract：:Cryptosporidium parvum is a protozoan parasite of the intestinal epithelium that has caused numerous outbreaks of diarrheal illness in humans. During our studies of the host immune response to C. parvum infection, we noted that two of the immunodominant surface antigens of the sporozoite stage of the parasite readily ...

journal_title：Molecular and biochemical parasitology

authors： Priest JW,Xie LT,Arrowood MJ,Lammie PJ

更新日期：2001-03-01 00:00:00

abstract：:Triclabendazole (TCBZ) is widely used for control of Fasciola hepatica (liver fluke) in animals and humans and resistance to this drug is now widespread. However, the mechanism of resistance to TCBZ is not known. A T687G single nucleotide polymorphism (SNP) in a P-glycoprotein gene was proposed as a molecular marker f...

journal_title：Molecular and biochemical parasitology

authors： Elliott TP,Spithill TW

更新日期：2014-11-01 00:00:00

abstract：:P-glycoprotein gene amplification has been described in several drug-resistant parasitic protozoa. The first P-glycoprotein related gene described in Leishmania was ltpgpA, a gene frequently amplified in arsenite resistant Leishmania. Hybridization experiments indicated that ltpgpA was part of a gene family. In additi...

journal_title：Molecular and biochemical parasitology

authors： Légaré D,Hettema E,Ouellette M

更新日期：1994-11-01 00:00:00

abstract：:After a single intravenous injection of suramin the rate of removal of the drug from the plasma into other tissue compartments of the rat is independent of initial concentration. The data can be fitted to the sum of two exponential functions, consistent with a two-compartment, open model system. Trypanosomes take up o...

journal_title：Molecular and biochemical parasitology

authors： Fairlamb AH,Bowman IB

更新日期：1980-10-01 00:00:00

abstract：:Plasmodium falciparum trophozoites were isolated by mechanical rupture of infected human erythrocytes followed by a series of differential centrifugation steps. After lysis with sonication, the 100 000 x g supernatant of parasites and uninfected host cells was used to determine the specific activities of a number of e...

journal_title：Molecular and biochemical parasitology

authors： Reyes P,Rathod PK,Sanchez DJ,Mrema JE,Rieckmann KH,Heidrich HG

更新日期：1982-05-01 00:00:00

abstract：:The origin of membraneous structures in the cytoplasm of human erythrocytes infected with the malaria parasite, Plasmodium falciparum, was determined by confocal fluorescence imaging microscopy. When infectious merozoites invaded erythrocytes labeled with the fluorescent, lipophilic, non-exchangeable molecules DiIC16 ...

journal_title：Molecular and biochemical parasitology

authors： Pouvelle B,Gormley JA,Taraschi TF

更新日期：1994-07-01 00:00:00

abstract：:The complete sequences of the genomic small subunit ribosomal RNA gene from two Phytomonas isolates: one associated with palm pathologies (P. cocos FGuiana) and one found in lactiferous plants with no apparent pathology (P. Euphorbe Senegal), were analyzed. Partial sequences from a number of other Phytomonas isolates ...

journal_title：Molecular and biochemical parasitology

authors： Marché S,Roth C,Philippe H,Dollet M,Baltz T

更新日期：1995-04-01 00:00:00