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Zinc finger nuclease technology: A stable tool for high efficiency transformation in bloodstream form T. brucei.

Abstract：

:In Trypanosoma brucei, the generation of knockout mutants is relatively easy compared to other organisms as transfection methods are well established. These methods have their limitations, however, when it comes to the generation of genome-wide libraries that require a minimum of several hundred thousand transformants. Double-strand breaks with the meganuclease ISce-I dramatically increase transformation efficiency, but are not widely in use as cell lines need to be generated de novo before each transfection. Here we show that zinc finger nucleases are a robust and stable tool that can enhance transformation in bloodstream forms by more than an order of magnitude.

journal_name

Mol Biochem Parasitol

journal_title

Molecular and biochemical parasitology

authors

Schumann G,Kangussu-Marcolino MM,Doiron N,Käser S,de Assis Burle-Caldas G,DaRocha WD,Teixeira SM,Roditi I

doi

10.1016/j.molbiopara.2017.02.002

subject

Has Abstract

pub_date

2017-04-01 00:00:00

pages

12-15

eissn

0166-6851

issn

1872-9428

pii

S0166-6851(17)30018-X

journal_volume

213

pub_type

杂志文章

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