Hydrogen peroxide metabolism in Trypanosoma brucei.

abstract：:Plasmodium falciparum merozoites invade erythrocytes using a range of alternative ligands that includes erythrocyte binding antigenic proteins (EBAs) and reticulocyte binding protein homologues (Rh). Variation in the expression of some of these genes among culture-adapted parasite lines correlates with the use of diff...

journal_title：Molecular and biochemical parasitology

authors： Nery S,Deans AM,Mosobo M,Marsh K,Rowe JA,Conway DJ

更新日期：2006-10-01 00:00:00

abstract：:The usefulness of random amplified polymorphic DNA markers (RAPD) was assayed in an attempt to discriminate among species, strains and individuals within the genus Schistosoma. Depending on the species, 40-50 arbitrary decamer oligonucleotides were used as primers to amplify total DNA by the polymerase chain reaction ...

journal_title：Molecular and biochemical parasitology

authors： Barral V,This P,Imbert-Establet D,Combes C,Delseny M

更新日期：1993-06-01 00:00:00

abstract：:Electron paramagnetic resonance spectrometry (EPR) techniques revealed a complex set of redox components in intact respiring cells of Tritrichomonas foetus, an aerotolerant anaerobic protozoon which lacks mitochondria. One of the two main systems of terminal respiration of this organism is the hydrogenosomal system, w...

journal_title：Molecular and biochemical parasitology

authors： Ohnishi T,Lloyd D,Lindmark DG,Müller M

更新日期：1980-10-01 00:00:00

abstract：:[14C]Artemisinin was taken up by Plasmodium falciparum in culture and concentrated in hemozoin. In vitro, hemin and artemisinin were found to undergo a chemical reaction forming two major products which were isolated by high-performance liquid chromatography (HPLC). The m/z values of the two products were 856 and 871....

journal_title：Molecular and biochemical parasitology

authors： Hong YL,Yang YZ,Meshnick SR

更新日期：1994-01-01 00:00:00

abstract：:We have assessed the phylogenetic status of the Trypanosoma cruzi Genome Project CL Brener reference strain by multilocus enzyme electrophoresis (MLEE) and multiprimer random amplified polymorphic DNA (RAPD) including a set of cloned stocks representative of the whole genetic diversity of T. cruzi. MLEE and RAPD data ...

journal_title：Molecular and biochemical parasitology

authors： Brisse S,Barnabé C,Bañuls AL,Sidibé I,Noël S,Tibayrenc M

更新日期：1998-05-01 00:00:00

abstract：:This study on isolated cercarial bodies demonstrates that the biological transformation from cercaria to schistosomulum and the biochemical transition from an aerobic to an anaerobic energy metabolism are separate processes, which are not necessarily linked. The metabolic transition depends on the external glucose con...

journal_title：Molecular and biochemical parasitology

authors： Horemans AM,Tielens AG,van den Bergh SG

更新日期：1992-03-01 00:00:00

abstract：:EP and GPEET procyclins are the major surface glycoproteins of Trypanosoma brucei in the midgut of tsetse flies (Glossina spp.). The procyclin genes are located at the beginning of polycistronic transcription units and are followed by at least one procyclin-associated gene (PAG). The EP/PAG1 locus on one copy of chrom...

journal_title：Molecular and biochemical parasitology

authors： Haenni S,Renggli CK,Fragoso CM,Oberle M,Roditi I

更新日期：2006-12-01 00:00:00

abstract：:The sequence of two fragments derived from the variable region of the kinetoplast maxicircle of Trypanosoma brucei has been determined. One fragment (1334 nucleotides, situated immediately upstream of the 12S and 9S ribosomal RNA genes) consists of non-repetitious DNA, which does not hybridize to other maxicircle regi...

journal_title：Molecular and biochemical parasitology

authors： de Vries BF,Mulder E,Brakenhoff JP,Sloof P,Benne R

更新日期：1988-01-01 00:00:00

abstract：:Trichuris arvicolae and T. muris are gastro-intestinal nematodes of respectively arvicoline and murine rodents. We aim to investigate the ecology of these Trichuris species using population genetics. We sampled nematodes from rodents trapped in the East of France. After confirming the species identification of the nem...

journal_title：Molecular and biochemical parasitology

authors： Deter J,Galan M,Charbonnel N,Cosson JF

更新日期：2009-10-01 00:00:00

abstract：:The in vitro-released 'excretory/secretory' (ES) and somatic antigens of the third stage (infective) larva of Anisakis simplex were characterised by radioiodination, immunoprecipitation, and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Oral infection with the parasite elicited a substantial IgG antibody...

journal_title：Molecular and biochemical parasitology

authors： Kennedy MW,Tierney J,Ye P,McMonagle FA,McIntosh A,McLaughlin D,Smith JW

更新日期：1988-10-01 00:00:00

abstract：:Malaria parasites export 'a secretome' of hundreds of proteins, including major virulence determinants, from their endoplasmic reticulum (ER), past the parasite plasma and vacuolar membranes to the host erythrocyte. The export mechanism is high affinity (nanomolar) binding of a host (cell) targeting (HT) motif RxLxE/D...

journal_title：Molecular and biochemical parasitology

authors： Bhattacharjee S,Speicher KD,Stahelin RV,Speicher DW,Haldar K

更新日期：2012-10-01 00:00:00

abstract：:Studies were made of the effects of diamphenethide-amine on glucose transport, glycogen breakdown, adenine nucleotides, metabolites and excretory products in both parenchymal and bile duct flukes in vitro and in bile duct flukes in vivo. The most consistent and pronounced effect observed was an elevation of malate lev...

journal_title：Molecular and biochemical parasitology

authors： Edwards SR,Campbell AJ,Sheers M,Moore RJ,Montague PE

更新日期：1981-04-01 00:00:00

abstract：:Strongyloides and related genera are advantageous subjects for transgenesis in parasitic nematodes, primarily by gonadal microinjection as has been used with Caenorhabditis elegans. Transgenesis has been achieved in Strongyloides stercoralis and in Parastrongyloides trichosuri, but both of these lack well-adapted, con...

journal_title：Molecular and biochemical parasitology

authors： Li X,Shao H,Junio A,Nolan TJ,Massey HC Jr,Pearce EJ,Viney ME,Lok JB

更新日期：2011-10-01 00:00:00

abstract：:The human malarial parasite Plasmodium falciparum secretes a histidine-rich protein (HRP-II) from infected erythrocytes. HRP-II has a very high content of histidine (H) (34%), alanine (A) (37%) and aspartic acid (D) (10%) and many contiguous repeats of the sequences AHH and AHHAAD. The histidine content of the protein...

journal_title：Molecular and biochemical parasitology

authors： Panton LJ,McPhie P,Maloy WL,Wellems TE,Taylor DW,Howard RJ

更新日期：1989-06-15 00:00:00

abstract：:The intraerythrocytic malaria parasite digests considerable amounts of its host cell cytosol, which consists mostly of hemoglobin. In order to avert the toxicity of ferriprotorphyrin IX (FP) thus produced, it is generally accepted that FP is polymerized to the non-toxic hemozoin. Investigating the fate of FP in cultur...

journal_title：Molecular and biochemical parasitology

authors： Zhang J,Krugliak M,Ginsburg H

更新日期：1999-03-15 00:00:00

abstract：:Repeated DNA sequences have been instrumental in the development of DNA probes for many different parasites. Isolation of such DNA probes has generally been accomplished by differential screening of genomic libraries with total genomic DNA preparations. In the current work, a rational design strategy is presented for ...

journal_title：Molecular and biochemical parasitology

authors： Zimmerman PA,Toe L,Unnasch TR

更新日期：1993-04-01 00:00:00

abstract：:Leishmania major aquaglyceroporin (AQP1) is an adventitious metalloid channel that allows the bidirectional movement of arsenite and antimonite. Here we demonstrate that AQP1 is subjected to proteasome-dependent degradation. Treatment of Leishmania promastigotes with the proteasome inhibitor MG132 resulted in increase...

journal_title：Molecular and biochemical parasitology

authors： Sharma M,Mandal G,Mandal S,Bhattacharjee H,Mukhopadhyay R

更新日期：2015-06-01 00:00:00

abstract：:A total of 518 expressed sequence tags (ESTs) have been generated from clones randomly selected from a cDNA library and a spliced leader sub-library of a Trypanosoma brucei rhodesiense bloodstream clone. 205 (39%) of the clones were identified based on matches to 113 unique genes in the public databases. Of these, 71 ...

journal_title：Molecular and biochemical parasitology

authors： el-Sayed NM,Alarcon CM,Beck JC,Sheffield VC,Donelson JE

更新日期：1995-07-01 00:00:00

abstract：:Plasmodium falciparum malaria parasites invade human red blood cells and immediately begin making significant alterations to the structure of the erythrocyte. These alterations facilitate the movement of nutrients into, and waste products and parasite-derived proteins out of the cell to meet the needs of the growing p...

journal_title：Molecular and biochemical parasitology

authors： Deitsch KW,Wellems TE

更新日期：1996-02-01 00:00:00

abstract：:Chloroquine is a critically important antimalarial drug and a well known intercalator into DNA. We now show that chloroquine binds more avidly to poly(dG-dC).poly(dG-dC) than to other synthetic polynucleotides and that this binding inhibits both salt- and cobalt-induced transitions to Z-DNA. These data are consistent ...

journal_title：Molecular and biochemical parasitology

authors： Kwakye-Berko F,Meshnick S

更新日期：1990-03-01 00:00:00

abstract：:Most of the signaling pathways are regulated by reversible phosphorylation-dephosphorylation which involves enzymes- kinases and phosphatases. Current knowledge about the protein phosphatases in parasites like Trypanosoma and Leishmania is very minimal despite their enormousity. In present study, full length ORF of Le...

journal_title：Molecular and biochemical parasitology

authors： Jakkula P,Qureshi R,Iqbal A,Sagurthi SR,Qureshi IA

更新日期：2018-07-01 00:00:00

abstract：:Cryptosporidium parvum is a protozoan parasite which produces self-limited disease in immunocompetent hosts and devastating, persistent diarrhea in immunocompromised individuals. There is no effective treatment for cryptosporidiosis and little is known about the basic biology of the organism. Cloning and sequence anal...

journal_title：Molecular and biochemical parasitology

authors： Barnes DA,Bonnin A,Huang JX,Gousset L,Wu J,Gut J,Doyle P,Dubremetz JF,Ward H,Petersen C

更新日期：1998-10-30 00:00:00

abstract：:A family of Schistosoma mansoni proteins (18-22 kDa, pI 5.3-5.8) are biosynthesized in juvenile worms and immunoprecipitated by antibodies uniquely present in protective Fischer rat antiserum. A cDNA clone, lambda gt11-40, expressing epitopes common to this protein family was used to obtain a genomic DNA clone, by hyb...

journal_title：Molecular and biochemical parasitology

authors： el-Sherbeini M,Ramadan N,Bostian KA,Knopf PM

更新日期：1991-11-01 00:00:00

abstract：:Nucleotide-sugar transporters (NSTs) transport activated sugars (e.g. UDP-GlcNAc) from the cytosol to the lumen of the endoplasmic reticulum or Golgi apparatus where they are used to make glycoproteins and glycolipids. UDP-Glc is an important component of the N-glycan-dependent quality control (QC) system for protein ...

journal_title：Molecular and biochemical parasitology

authors： Banerjee S,Cui J,Robbins PW,Samuelson J

更新日期：2008-05-01 00:00:00

abstract：:Peptidomimetic inhibitors of mammalian zinc metalloproteases have been tested as potential agents for intervention in disease caused by kinetoplastid protozoa. Certain metalloprotease inhibitors were able to inhibit the release of variant surface glycoprotein from cultured transgenic procyclic Trypanosoma brucei, conf...

journal_title：Molecular and biochemical parasitology

authors： Bangs JD,Ransom DA,Nimick M,Christie G,Hooper NM

更新日期：2001-04-25 00:00:00

abstract：:The complete sequence for a major Schistosome mansoni eggshell protein gene has been determined from a genomic DNA fragment. The use of an open reading frame encoding a glycine-rich polypeptide was confirmed by in vitro translation of schistosome mRNA in the presence of [3H]glycine and comparison with the amino acid c...

journal_title：Molecular and biochemical parasitology

authors： Rodrigues V,Chaudhri M,Knight M,Meadows H,Chambers AE,Taylor WR,Kelly C,Simpson AJ

更新日期：1989-01-01 00:00:00

abstract：:The technique of RNA interference (RNAi) is exceedingly useful for knocking down the expression of a specific mRNA in African trypanosomes and other organisms for the purpose of examining the function of its gene. However, when we attempted to apply RNAi in the Latin American trypanosome, Trypanosoma cruzi, to diminis...

journal_title：Molecular and biochemical parasitology

authors： DaRocha WD,Otsu K,Teixeira SM,Donelson JE

更新日期：2004-02-01 00:00:00

abstract：:The nucleotide sequence of a gene encoding a 35-kDa thiol protease of the parasitic nematode Haemonchus contortus has been determined. The gene, designated AC-2, shares 97% nucleotide sequence identity and 98% amino acid identity with previously characterized AC-1 cDNAs encoding the thiol protease. The AC-2 gene spans...

journal_title：Molecular and biochemical parasitology

authors： Pratt D,Cox GN,Milhausen MJ,Boisvenue RJ

更新日期：1990-12-01 00:00:00

abstract：:Evidence is presented that Giardia lamblia and Entamoeba histolytica, phylogenetically unrelated aerotolerant anaerobes, have crucial thiol groups on or easily accessible to their external surface. Both parasites were killed by three structurally unrelated thiol-blocking reagents which penetrate intact cells poorly or...

journal_title：Molecular and biochemical parasitology

authors： Gillin FD,Reiner DS,Levy RB,Henkart PA

更新日期：1984-09-01 00:00:00

abstract：:A Babesia bovis merozoite protein, Bb-1, was localized by immunoelectron microscopy to an apical organelle known as the spherical body. This unique structure appears to be analogous to dense granules of other apicomplexan protozoa. Similar to previously described dense granule proteins of Plasmodium spp., Bb-1 is secr...

journal_title：Molecular and biochemical parasitology

authors： Hines SA,Palmer GH,Brown WC,McElwain TF,Suarez CE,Vidotto O,Rice-Ficht AC

更新日期：1995-02-01 00:00:00