Sequence preference of chloroquine binding to DNA and prevention of Z-DNA formation.

abstract：:Benznidazole is the main drug used to treat Trypanosoma cruzi infections. However, frequent instances of treatment failure have been reported. To better understand potential resistance mechanisms, we analysed three clones isolated from a single parasite population that had undergone benznidazole-selection. These clone...

journal_title：Molecular and biochemical parasitology

authors： Campos MC,Leon LL,Taylor MC,Kelly JM

更新日期：2014-01-01 00:00:00

abstract：:The infection of human erythrocytes by two strains of the human malarial parasite, Plasmodium falciparum (FCQ-27 or the multi-drug-resistant strain K-1), markedly changed the transport characteristics of the nucleosides, adenosine and tubercidin, compared to uninfected erythrocytes. A component of the transport of the...

journal_title：Molecular and biochemical parasitology

authors： Gero AM,Scott HV,O'Sullivan WJ,Christopherson RI

更新日期：1989-04-01 00:00:00

abstract：:Leishmania parasites cycle between the alimentary tract of a sandfly vector as free-living promastigotes and the acidified phagolysosomes of the vertebrate host macrophage as aflagellated amastigotes. The differentiation process can be mimicked in host-free culture by switching promastigotes (e.g. 25 degrees C, neutra...

journal_title：Molecular and biochemical parasitology

authors： Rochette A,Raymond F,Corbeil J,Ouellette M,Papadopoulou B

更新日期：2009-05-01 00:00:00

abstract：:A complex of non-covalently bound polypeptides is located on the surface of the merozoite form of the human malaria parasite Plasmodium falciparum. Four of these polypeptides are derived by proteolytic processing of the merozoite surface protein 1 (MSP-1) precursor. Two components, a 22 and a 36 kDa polypeptide are no...

journal_title：Molecular and biochemical parasitology

authors： Trucco C,Fernandez-Reyes D,Howell S,Stafford WH,Scott-Finnigan TJ,Grainger M,Ogun SA,Taylor WR,Holder AA

更新日期：2001-01-15 00:00:00

abstract：:Soon after infecting a mammalian host, cercariae of Schistosoma mansoni rapidly undergo a series of morphologic and biochemical adaptations associated with transformation to their next developmental stage, the schistosomulum. Few of these changes are associated with alterations in gene expression except for an apparen...

journal_title：Molecular and biochemical parasitology

authors： Blanton RE,Licate LS

更新日期：1992-04-01 00:00:00

abstract：:Naturally occurring sequences containing repetitive guanine motifs have the potential to form tetraplex DNA. Phytomonas serpens minicircle DNA shows some regions where one strand is composed mainly of G and T (GT regions). These regions contain several stretches of contiguous guanines. An oligonucleotide was construct...

journal_title：Molecular and biochemical parasitology

authors： Sá-Carvalho D,Traub-Cseko YM

更新日期：1995-06-01 00:00:00

abstract：:A Fasciola hepatica cDNA clone of 1752 bp was isolated from an adult worm cDNA expression library by immunological screening using a rabbit serum against the excretory-secretory antigens. The nucleotide sequence of the cDNA revealed the presence of an open reading frame of 489 codons which encoded a 55 kDa polypeptide...

journal_title：Molecular and biochemical parasitology

authors： Salazar-Calderón M,Martín-Alonso JM,Castro AM,Parra F

更新日期：2003-01-01 00:00:00

abstract：:Among several protease inhibitors tested, only leupeptin was found to modify qualitatively the processing of P126, a major antigen of the parasitophorous vacuole of Plasmodium falciparum, and to inhibit the release of merozoites. Whereas P126 is normally processed upon merozoite release into 2 polypeptides of 50 and 7...

journal_title：Molecular and biochemical parasitology

authors： Debrabant A,Delplace P

更新日期：1989-03-01 00:00:00

abstract：:The Trypanosoma brucei flagellum controls motility and is crucial for cell polarity and division. Unique features of trypanosome motility suggest that flagellar beat regulation in this organism is unusual and worthy of study. The flagellar axoneme, required for motility, has a structure that is highly conserved among ...

journal_title：Molecular and biochemical parasitology

authors： Springer AL,Bruhn DF,Kinzel KW,Rosenthal NF,Zukas R,Klingbeil MM

更新日期：2011-01-01 00:00:00

abstract：:The activity of pyrimethamine and sulfadoxine against two strains of Plasmodium falciparum has been studied in vitro by a radioisotopic technique. Low level antagonism of pyrimethamine resulted from the inclusion of p-aminobenzoic acid, p-aminobenzoylglutamic acid or folic acid in the test medium. Sulfadoxine activity...

journal_title：Molecular and biochemical parasitology

authors： Watkins WM,Sixsmith DG,Chulay JD,Spencer HC

更新日期：1985-01-01 00:00:00

abstract：:The glycosylphosphatidylinositol phospholipase C (GPI-PLC) from Trypanosoma brucei is particularly effective in hydrolysing the GPI-anchors of some proteins. The enzyme is inhibited by Zn2+ and p-chloromercurylphenylsulphonic acid, both of which can act as sulphydryl reagents, suggesting that a cysteine residue may be...

journal_title：Molecular and biochemical parasitology

authors： Carnall N,Webb H,Carrington M

更新日期：1997-12-15 00:00:00

abstract：:Polyacrylamide gel electrophoresis of the total RNA extracted from cultures of Plasmodium falciparum resolved two major RNA components with estimated molecular weights of 1.3 X 10(6) and 0.72 - 0.74 X 10(6). Oligo(dT)-cellulose column chromatography of the total cellular RNA indicates that more than 90% of the total r...

journal_title：Molecular and biochemical parasitology

authors： Vezza AC,Trager W

更新日期：1981-12-01 00:00:00

abstract：:Entamoeba histolytica is a human intestinal parasite that causes amebic dysentery. A cell surface amebic adhesin, the galactose and N-acetyl-D-galactosamine inhibitable (GalNAc) lectin mediates amebic adherence to and contact-dependent killing of host cells. Previous work has suggested that the GalNAc lectin transduce...

journal_title：Molecular and biochemical parasitology

authors： Hughes MA,Lee CW,Holm CF,Ghosh S,Mills A,Lockhart LA,Reed SL,Mann BJ

更新日期：2003-04-03 00:00:00

abstract：:Many of the currently available nematicides used in nematode control are hazardous to the user, environment and beneficial non-target organisms. Therefore the need to develop alternative methods for nematode control such as the development of nematode-resistant crops through RNA-mediated interference (RNAi) holds grea...

journal_title：Molecular and biochemical parasitology

authors： Joseph S,Gheysen G,Subramaniam K

更新日期：2012-11-01 00:00:00

abstract：:Toxoplasma gondii differentially expresses two forms of lactate dehydrogenase in tachyzoites and bradyzoites, respectively, designated LDH1 and LDH2. Previously it was demonstrated that LDH1 and LDH2 share a unique structural feature with LDH from the malarial parasite Plasmodium falciparum (pLDH), namely, the additio...

journal_title：Molecular and biochemical parasitology

authors： Dando C,Schroeder ER,Hunsaker LA,Deck LM,Royer RE,Zhou X,Parmley SF,Vander Jagt DL

更新日期：2001-11-01 00:00:00

abstract：:To investigate whether the lysosomes of Trypanosoma brucei are capable of uptake of macromolecules after internalization by the cell, we used Triton WR-1339, a non-digestible macromolecular compound, which is known to cause a marked decrease in the density of hepatic lysosomes due to massive intralysosomal storage. In...

journal_title：Molecular and biochemical parasitology

authors： Opperdoes FR,Van Roy J

更新日期：1982-09-01 00:00:00

abstract：:Cyclic AMP-dependent protein kinase (protein kinase A, PKA) is a key element in many cell signaling pathways. An essential role of Plasmodium falciparum PKA (PfPKA) activity was reported in the intraerythrocytic growth of the malaria parasite. However, molecular characterization of PfPKA using purified recombinant pro...

journal_title：Molecular and biochemical parasitology

authors： Sudo A,Kato K,Kobayashi K,Tohya Y,Akashi H

更新日期：2008-08-01 00:00:00

abstract：:Intra-erythrocytic Plasmodium parasites digest host cell haemoglobin and use the liberated amino acids for protein synthesis. Although several endoproteases (aspartic, cysteine, and metallo-) have been shown to be involved in the initial stages of haemoglobin degradation, little is known about the steps immediately be...

journal_title：Molecular and biochemical parasitology

authors： Gavigan CS,Dalton JP,Bell A

更新日期：2001-09-28 00:00:00

abstract：:Translation initiation factor eIF4F is essential for cap-dependent translation initiation in eukaryotes. eIF4F is a trimeric complex consisting of a scaffold protein eIF4G, cap-binding protein eIF4E and DEAD-box RNA helicase eIF4A. eIF4F binds to the 5' cap structure of the mRNA through eIF4E and facilitates the bindi...

journal_title：Molecular and biochemical parasitology

authors： Adedoja AN,McMahan T,Neal JP,Hamal Dhakal S,Jois S,Romo D,Hull K,Garlapati S

更新日期：2020-03-01 00:00:00

abstract：:Root-knot nematodes (Meloidogyne spp.) are among the most important plant pathogens. In this study, the mitochondrial (mt) genomes of the root-knot nematodes, M. chitwoodi and M. incognita were sequenced. PCR analyses suggest that both mt genomes are circular, with an estimated size of 19.7 and 18.6-19.1kb, respective...

journal_title：Molecular and biochemical parasitology

authors： Humphreys-Pereira DA,Elling AA

更新日期：2014-03-01 00:00:00

abstract：:Phosphoinositides comprise a group of essential phospholipids that control a variety of cellular functions. In the case of the human malaria parasite Plasmodium falciparum, phosphoinositides have been shown to trigger exflagellation and to affect haemoglobin endocytosis and maturation of the parasite's digestive vacuo...

journal_title：Molecular and biochemical parasitology

authors： Krüger T,Sanchez CP,Lanzer M

更新日期：2010-08-01 00:00:00

abstract：:Plasma membrane vesicles of Trypanosoma cruzi (PMVs) formed saturation binding isotherms with naive murine T lymphocytes. Parasite membrane attachment to the muscarinic cholinergic receptors of Lyt 2.2+T cells (suppressor cells) resulted in the synthesis of cGMP, attenuation of cAMP levels and in the secretion of pros...

journal_title：Molecular and biochemical parasitology

authors： Borda ES,Sterin-Borda LJ,Pascual JO,Gorelik G,Felix JC,von Kreuter BF,Santos-Buch CA

更新日期：1991-07-01 00:00:00

abstract：:The gender of cercarial larvae can only be determined using molecular methods. End point PCR methods that amplify repetitive markers on the W chromosome of the female (ZW) parasites have been developed, but sometimes results are ambiguous or incorrect. To more effectively distinguish sexes, and to determine why end po...

journal_title：Molecular and biochemical parasitology

authors： Chevalier FD,Le Clec'h W,Alves de Mattos AC,LoVerde PT,Anderson TJ

更新日期：2016-01-01 00:00:00

abstract：:In order to clarify the interpretation of molecular karyotype polymorphisms in Leishmania, the three smallest chromosomes from five cloned strains of Leishmania infantum were identified by chromosome-specific anonymous DNA probes. The presence in the same clone of homologous chromosomes of a different size was demonst...

journal_title：Molecular and biochemical parasitology

authors： Blaineau C,Bastien P,Rioux JA,Roizès G,Pagès M

更新日期：1991-06-01 00:00:00

abstract：:A total of 518 expressed sequence tags (ESTs) have been generated from clones randomly selected from a cDNA library and a spliced leader sub-library of a Trypanosoma brucei rhodesiense bloodstream clone. 205 (39%) of the clones were identified based on matches to 113 unique genes in the public databases. Of these, 71 ...

journal_title：Molecular and biochemical parasitology

authors： el-Sayed NM,Alarcon CM,Beck JC,Sheffield VC,Donelson JE

更新日期：1995-07-01 00:00:00

abstract：:Topoisomerases are enzymes that participate in many cellular functions involving topological manipulation of DNA strands. There are two types of topoisomerases in the cell: (a) type I topoisomerases; and (b) type II topoisomerases (topo II). Previously we have cloned and sequenced the gene encoding Trypanosoma cruzi t...

journal_title：Molecular and biochemical parasitology

authors： Fragoso SP,Mattei D,Hines JC,Ray D,Goldenberg S

更新日期：1998-08-01 00:00:00

abstract：:The human malarial parasite Plasmodium falciparum secretes a histidine-rich protein (HRP-II) from infected erythrocytes. HRP-II has a very high content of histidine (H) (34%), alanine (A) (37%) and aspartic acid (D) (10%) and many contiguous repeats of the sequences AHH and AHHAAD. The histidine content of the protein...

journal_title：Molecular and biochemical parasitology

authors： Panton LJ,McPhie P,Maloy WL,Wellems TE,Taylor DW,Howard RJ

更新日期：1989-06-15 00:00:00

abstract：:The major proteolytic activity of Trypanosoma cruzi is a cathepsin L-like cysteine protease expressed in all stages of the parasite. As an initial step in identifying possible functions of this enzyme in the life cycle of T. cruzi, and examining its potential as a target for rational drug design, two fluoromethyl keto...

journal_title：Molecular and biochemical parasitology

authors： Harth G,Andrews N,Mills AA,Engel JC,Smith R,McKerrow JH

更新日期：1993-03-01 00:00:00

abstract：:Ribosome biosynthesis, best studied in opisthokonts, is a highly complex process involving numerous protein and RNA factors. Yet, very little is known about the early stages of pre-18S rRNA processing even in these model organisms, let alone the conservation of this mechanism in other eukaryotes. Here we extend our kn...

journal_title：Molecular and biochemical parasitology

authors： Faktorová D,Bär A,Hashimi H,McKenney K,Horák A,Schnaufer A,Rubio MAT,Alfonzo JD,Lukeš J

更新日期：2018-10-01 00:00:00

abstract：:The expressed sequence tag (EST) dataset of Toxoplasma gondii provides a wealth of information towards gene discovery. The complete cDNA and genomic sequence of EST tgc050 locus shows that it contains five copies of the conserved thrombospondin (TSP)-like motif present in a number of molecules with adhesive properties...

journal_title：Molecular and biochemical parasitology

authors： Wan KL,Carruthers VB,Sibley LD,Ajioka JW

更新日期：1997-02-01 00:00:00