Mitochondrial genomes of Meloidogyne chitwoodi and M. incognita (Nematoda: Tylenchina): comparative analysis, gene order and phylogenetic relationships with other nematodes.


:Root-knot nematodes (Meloidogyne spp.) are among the most important plant pathogens. In this study, the mitochondrial (mt) genomes of the root-knot nematodes, M. chitwoodi and M. incognita were sequenced. PCR analyses suggest that both mt genomes are circular, with an estimated size of 19.7 and 18.6-19.1kb, respectively. The mt genomes each contain a large non-coding region with tandem repeats and the control region. The mt gene arrangement of M. chitwoodi and M. incognita is unlike that of other nematodes. Sequence alignments of the two Meloidogyne mt genomes showed three translocations; two in transfer RNAs and one in cox2. Compared with other nematode mt genomes, the gene arrangement of M. chitwoodi and M. incognita was most similar to Pratylenchus vulnus. Phylogenetic analyses (Maximum Likelihood and Bayesian inference) were conducted using 78 complete mt genomes of diverse nematode species. Analyses based on nucleotides and amino acids of the 12 protein-coding mt genes showed strong support for the monophyly of class Chromadorea, but only amino acid-based analyses supported the monophyly of class Enoplea. The suborder Spirurina was not monophyletic in any of the phylogenetic analyses, contradicting the Clade III model, which groups Ascaridomorpha, Spiruromorpha and Oxyuridomorpha based on the small subunit ribosomal RNA gene. Importantly, comparisons of mt gene arrangement and tree-based methods placed Meloidogyne as sister taxa of Pratylenchus, a migratory plant endoparasitic nematode, and not with the sedentary endoparasitic Heterodera. Thus, comparative analyses of mt genomes suggest that sedentary endoparasitism in Meloidogyne and Heterodera is based on convergent evolution.


Mol Biochem Parasitol


Humphreys-Pereira DA,Elling AA




Has Abstract


2014-03-01 00:00:00














  • Characterisation of the novel junctions of two minichromosomes of Leishmania major.

    abstract::The minichromosomes M210 and M230 of Leishmania major are linear amplicons which have an inverted repeat structure. We report here the molecular cloning of the megabasic chromosome region where M210 and M230 recombination points are located. The analysis of this chromosomal region revealed a 374 bp fragment directly r...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ortiz G,Segovia M

    更新日期:1996-11-25 00:00:00

  • Developmental expression of high molecular weight tropomyosin isoforms in Mesocestoides corti.

    abstract::Tropomyosins are a family of actin-binding proteins with diverse roles in actin filament function. One of the best characterized roles is the regulation of muscle contraction. Tropomyosin isoforms can be generated from different genes, and from alternative promoters and alternative splicing from the same gene. In this...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Koziol U,Costábile A,Domínguez MF,Iriarte A,Alvite G,Kun A,Castillo E

    更新日期:2011-02-01 00:00:00

  • Immunological identification of Schistosoma mansoni peptidases.

    abstract::Precipitin arcs formed after immunoelectrophoresis of Schistosoma mansoni egg, worm and cercarial antigens with polyspecific rabbit antisera have been 'stained' at neutral pH with chromogenic enzyme substrates specific for proteases and peptidases. A total of 7 antigenically distinct enzymes with peptidolytic properti...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Doenhoff MJ,Modha J,Curtis RH,Adeoye GO

    更新日期:1988-12-01 00:00:00

  • 5'-p-Fluorosulfonyl benzoyl adenosine inhibits an ecto-ATP-diphosphohydrolase in the tegument surface of Taenia crassiceps cysticerci.

    abstract::The tegumental membrane of Taenia crassiceps cysticerci contains an ATP-diphosphohydrolase (EC which hydrolyzes purine and pyrimidine nucleoside 5'-di- and 5'-triphosphates at an optimum pH of 8.5. It is Mg(2+)-dependent and insensitive to classical ATPase and phosphatase inhibitors. In solubilized tegumental...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Guevara-Flores A,Olvera-Sánchez S,Gómez-Concha C,Juárez O,Esparza-Perusquía M,Pardo JP,Mendoza-Hernández G,Martínez F,Flores-Herrera O

    更新日期:2008-12-01 00:00:00

  • Genomic organisation of nuclear tRNAGly and tRNALeu genes in Trypanosoma brucei.

    abstract::We have isolated a 0.3-kb HaeIII restriction fragment from Trypanosoma brucei which contains two tRNA genes. Secondary structure models predict that the two genes identified encode tRNA molecules which specify glycine (anticodon UCC) and leucine (anticodon CAG). The two genes are separated by 86 nucleotides, transcrib...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Campbell DA,Suyama Y,Simpson L

    更新日期:1989-12-01 00:00:00

  • Trypanosoma cruzi poly-zinc finger protein: a novel DNA/RNA-binding CCHC-zinc finger protein.

    abstract::A poly-zinc finger protein, designated PZFP1 was identified in Trypanosoma cruzi for the first time. The protein has 191 amino acids, contains seven motifs Cys(X)(2)Cys(X)(4)His(X)(4)Cys. A recombinant PZFP1 was generated in E. coli and the expected 21kDa polypeptide co-purified with two other inducible products of ab...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Espinosa JM,Portal D,Lobo GS,Pereira CA,Alonso GD,Gómez EB,Lan GH,Pomar RV,Flawiá MM,Torres HN

    更新日期:2003-09-01 00:00:00

  • Ecdysteroids in adults of the nematode, Dirofilaria immitis.

    abstract::Adult males and females of the dog heartworm, Dirofilaria immitis, were extracted separately and, following separation of the free and conjugated ecdysteroid fractions, the conjugates were hydrolysed enzymically. Both the ecdysteroids released by hydrolysis of the conjugates and the free hormones were further purified...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Mendis AH,Rose ME,Rees HH,Goodwin TW

    更新日期:1983-11-01 00:00:00

  • An S antigen gene from Plasmodium falciparum contains a novel repetitive sequence.

    abstract::The complete sequence of the gene coding for the S antigen from the Wellcome (West African) strain of Plasmodium falciparum has been obtained. It contains a central repetitive region consisting of 65 copies of a partially degenerate 24 bp sequence, coding for a conserved 8 amino acid repeat (Gly Pro Asn Ser Asp Gly As...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Nicholls SC,Hillman Y,Lockyer MJ,Odink KG,Holder AA

    更新日期:1988-02-01 00:00:00

  • Acute and persistent infection by a transfected Mo7 strain of Babesia bovis.

    abstract::A Mo7-derived Babesia bovis line stably transfected with the gfp-bsd gene was inoculated into two four to five months old calves, while two additional calves were inoculated with Mo7 parasites. Similar mild clinical signs were detected in all calves. B. bovis rap-1 was identified in the bloodstream by PCR four days po...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Suarez CE,Laughery JM,Schneider DA,Sondgeroth KS,McElwain TF

    更新日期:2012-09-01 00:00:00

  • Structural analysis of the acetaldehyde dehydrogenase activity of Entamoeba histolytica alcohol dehydrogenase 2 (EhADH2), a member of the ADHE enzyme family.

    abstract::The ADHE family of enzymes are bifunctional acetaldehyde dehydrogenase (ALDH)/alcohol dehydrogenase (ADH) enzymes that probably arose from the fusion of genes encoding separate ALDH and ADH enzymes. Here we have used the Entamoeba histolytica alcohol dehydrogenase 2 (EhADH2) enzyme as a prototype to analyze the struct...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Chen M,Li E,Stanley SL Jr

    更新日期:2004-10-01 00:00:00

  • Nuclear factors binding to the mini-exon repeat of Trypanosoma brucei.

    abstract::In trypanosomes and related kinetoplastida a common short 5' leader sequence is encoded by the highly repetitive mini-exon gene and added to all protein coding mRNAs by trans-splicing. To initiate a search for transcription factors associated with transcription of the mini-exon we have used an electrophoretic mobility...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Koronakis VE,Ross AM,Le Page RW,Hughes C

    更新日期:1988-07-01 00:00:00

  • Cloning and analysis of the RESA-2 gene: a DNA homologue of the ring-infected erythrocyte surface antigen gene of Plasmodium falciparum.

    abstract::We have cloned and sequenced a homologue of the ring-infected erythrocyte surface antigen (RESA) gene from Plasmodium falciparum designated RESA-2. Two reading frames with high homology to exon 1 and exon 2 of RESA at both the nucleotide and amino acid levels were identified in the RESA-2 sequence. However, RESA-2 doe...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Cappai R,Kaslow DC,Peterson MG,Cowman AF,Anders RF,Kemp DJ

    更新日期:1992-09-01 00:00:00

  • Molecular cloning, genomic structure and localization in a blood stage antigen of Plasmodium falciparum characterized by a serine stretch.

    abstract::Two short DNA segments were isolated by screening of a lambda gt11 library from Plasmodium falciparum schizont cDNA with an antiserum against the 140 kDa protein, which confers protective immunity to monkeys. The segments were used to identify a genomic fragment which carries the entire coding sequence for a protein o...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Knapp B,Hundt E,Nau U,Küpper HA

    更新日期:1989-01-01 00:00:00

  • cDNA expressed sequence tags of Trypanosoma brucei rhodesiense provide new insights into the biology of the parasite.

    abstract::A total of 518 expressed sequence tags (ESTs) have been generated from clones randomly selected from a cDNA library and a spliced leader sub-library of a Trypanosoma brucei rhodesiense bloodstream clone. 205 (39%) of the clones were identified based on matches to 113 unique genes in the public databases. Of these, 71 ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: el-Sayed NM,Alarcon CM,Beck JC,Sheffield VC,Donelson JE

    更新日期:1995-07-01 00:00:00

  • Rapid evolution of an erythrocyte invasion gene family: the Plasmodium reichenowi Reticulocyte Binding Like (RBL) genes.

    abstract::Malarial merozoites use an array of ligands, including members of the Reticulocyte Binding Like (RBL) super-family of invasion proteins, to identify and invade erythrocytes. RBL family members are large Type I membrane anchored proteins expressed at the invasive end of merozoites that share homology with the Reticuloc...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Rayner JC,Huber CS,Galinski MR,Barnwell JW

    更新日期:2004-02-01 00:00:00

  • Characterisation of the gene encoding a 104-kilodalton microneme-rhoptry protein of Theileria parva.

    abstract::A neutralizing antiserum, C16, raised against sporozoites of Theileria parva parva was used to screen a lambda gt11 expression library of T. parva parva (Muguga) genomic DNA fragments. Proteins encoded by one phage clone, lambda TpS-17, were reactive with the C16 antiserum. Detailed characterisation of the DNA insert ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Iams KP,Young JR,Nene V,Desai J,Webster P,ole-MoiYoi OK,Musoke AJ

    更新日期:1990-02-01 00:00:00

  • A (Ca(2+)-Mg2+)ATPase from Schistosoma mansoni is coupled to an active transport of calcium.

    abstract::The (Ca(2+)-Mg2+)ATPase activity in microsomes of Schistosoma mansoni is fully inhibited by vanadate (I50 = 2.5 microM). 45Ca2+ is accumulated within microsomal vesicles in an ATP-dependent process that is enhanced 5-fold in the presence of 40 mM phosphate. Accumulated 45Ca2+ is rapidly released by 5 microM of the Ca2...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Cunha VM,Meyer-Fernandes JR,Noël F

    更新日期:1992-06-01 00:00:00

  • Excretory/secretory products from in vitro-cultured Echinococcus granulosus protoscoleces.

    abstract::Cystic hydatid disease (CHD) is caused by infection with Echinococcus granulosus metacestodes and affects humans and livestock. Proteins secreted or excreted by protoscoleces, pre-adult worms found in the metacestode, are thought to play fundamental roles in the host-parasite relationship. In this work, we performed a...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Virginio VG,Monteiro KM,Drumond F,de Carvalho MO,Vargas DM,Zaha A,Ferreira HB

    更新日期:2012-05-01 00:00:00

  • A comparison of the enzymatic properties of the major cysteine proteinases from Trypanosoma congolense and Trypanosoma cruzi.

    abstract::Congopain and cruzipain, the major cysteine proteinases from Trypanosoma congolense and Trypanosoma cruzi, were compared for their activities towards a series of new, sensitive fluorogenic substrates of the papain family of cysteine proteinases and for their sensitivity to inhibition by cystatins and related biotinyla...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Chagas JR,Authie E,Serveau C,Lalmanach G,Juliano L,Gauthier F

    更新日期:1997-09-01 00:00:00

  • Purification, characterization, and immunochemical studies of beta-N-acetyl-D-hexosaminidase from the parasitic nematode Trichinella spiralis.

    abstract::The exoglycosidase, beta-N-acetyl-D-hexosaminidase was purified 600-fold from the muscle-stage larvae (L1) of Trichinella spiralis. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), the purified enzyme-active fraction contained 4 polypeptides with apparent molecular weights of 100,000, 68,000, 5...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Rhoads ML

    更新日期:1988-10-01 00:00:00

  • Three acetylcholinesterases of the pinewood nematode, Bursaphelenchus xylophilus: insights into distinct physiological functions.

    abstract::Acetylcholinesterase (AChE) plays a key role in postsynaptic transmission in most animals. Nematodes encode multiple AChEs, implying its functional diversity. To explore physiological functions of multiple AChEs, three distinct AChEs (BxACE-1, BxACE-2, and BxACE-3) were identified and characterized from the pinewood n...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kang JS,Lee DW,Choi JY,Je YH,Koh YH,Lee SH

    更新日期:2011-02-01 00:00:00

  • Leishmania donovani PP2C: Kinetics, structural attributes and in vitro immune response.

    abstract::Most of the signaling pathways are regulated by reversible phosphorylation-dephosphorylation which involves enzymes- kinases and phosphatases. Current knowledge about the protein phosphatases in parasites like Trypanosoma and Leishmania is very minimal despite their enormousity. In present study, full length ORF of Le...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Jakkula P,Qureshi R,Iqbal A,Sagurthi SR,Qureshi IA

    更新日期:2018-07-01 00:00:00

  • Cloning and functional identification of a novel annexin subfamily in Cysticercus cellulosae.

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hongli Y,Shuhan S,Ruiwen C,Yingjun G

    更新日期:2002-01-01 00:00:00

  • The interaction of artemisinin with malarial hemozoin.

    abstract::[14C]Artemisinin was taken up by Plasmodium falciparum in culture and concentrated in hemozoin. In vitro, hemin and artemisinin were found to undergo a chemical reaction forming two major products which were isolated by high-performance liquid chromatography (HPLC). The m/z values of the two products were 856 and 871....

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hong YL,Yang YZ,Meshnick SR

    更新日期:1994-01-01 00:00:00

  • Coordinate regulation of a family of promastigote-enriched mRNAs by the 3'UTR PRE element in Leishmania mexicana.

    abstract::Post-transcriptional regulation is a key feature controlling gene expression in the protozoan parasite Leishmania. The nine-nucleotide paraflagellar rod regulatory element (PRE) in the 3'UTR of Leishmania mexicana PFR2 is both necessary and sufficient for the observed 10-fold higher level of PFR2 mRNA in promastigotes...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Holzer TR,Mishra KK,LeBowitz JH,Forney JD

    更新日期:2008-01-01 00:00:00

  • Antigenic diversity of the circumsporozoite proteins in the Plasmodium cynomolgi complex.

    abstract::Antigenic diversity was observed in the circumsporozoite (CS) proteins of five of the six Plasmodium cynomolgi isolates (NIH, Mulligan, London, Gombak, Ceylon, RO) that we examined. Monoclonal antibodies were produced against salivary gland sporozoites of three of the isolates. Interaction of these monoclonal antibodi...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Cochrane AH,Gwadz RW,Ojo-Amaize E,Hii J,Nussenzweig V,Nussenzweig RS

    更新日期:1985-01-01 00:00:00

  • Substrate specificity of recombinant cysteine proteinase, CPB, of Leishmania mexicana.

    abstract::The primary S(1) subsite specificity of a recombinant cysteine proteinase, CPB2.8 Delta CTE, of Leishmania mexicana was investigated in a systematic way using a series of peptides derived from Abz-KLRFSKQ-EDDnp in which Arg was substituted by all natural amino acids (where Abz is ortho-amino-benzoyl and EDDnp is N-[2,...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Alves LC,Judice WA,St Hilaire PM,Meldal M,Sanderson SJ,Mottram JC,Coombs GH,Juliano L,Juliano MA

    更新日期:2001-08-01 00:00:00

  • Heat shock and stress response in Giardia lamblia.

    abstract::The heat shock response was studied as a model for control of gene expression and protein synthesis in Giardia lamblia. Cultured trophozoites were metabolically labelled with [35S]methionine, and proteins were analysed by SDS-polyacrylamide gel electrophoresis and fluorography. A temperature shift from 37 degrees C to...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Lindley TA,Chakraborty PR,Edlind TD

    更新日期:1988-03-01 00:00:00

  • Sequences required for the flagellar targeting of an integral membrane protein.

    abstract::Previous studies have established that the ISO1 glucose transporter of Leishmania enriettii resides primarily in the flagellar membrane, whereas the ISO2 glucose transporter is located in the pellicular plasma membrane surrounding the cell body. This pronounced difference in subcellular targeting is conferred by the N...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Nasser MI,Landfear SM

    更新日期:2004-05-01 00:00:00

  • Biochemical and functional characterization of CpMuc4, a Cryptosporidium surface antigen that binds to host epithelial cells.

    abstract::Cryptosporidium spp. are intracellular apicomplexan parasites that cause outbreaks of waterborne diarrheal disease worldwide. Previous studies had identified a Cryptosporidium parvum sporozoite antigen, CpMuc4, that appeared to be involved in attachment and invasion of the parasite into intestinal epithelial cells. Cp...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Paluszynski J,Monahan Z,Williams M,Lai O,Morris C,Burns P,O'Connor R

    更新日期:2014-02-01 00:00:00