The major 36 kDa Neospora caninum tachyzoite surface protein is closely related to the major Toxoplasma gondii surface antigen.

abstract：:The Trypanosoma cruzi insect stage-specific antigen GP72 was purified from epimastigotes and the amino acid sequences of peptide fragments determined. Oligonucleotides derived from these data were used to amplify and clone a cDNA sequence, which was used to isolate a full-length gene. All the sequenced peptides were e...

journal_title：Molecular and biochemical parasitology

authors： Cooper R,Inverso JA,Espinosa M,Nogueira N,Cross GA

更新日期：1991-11-01 00:00:00

abstract：:Adult Schistosoma mansoni worms rapidly degrade their endogenous glycogen stores immediately after isolation from the host. In NCTC 109 or in a diphasic culture medium the glycogen levels slowly recovered again after the initial decrease. The rapid degradation of glycogen could be prevented, even in a simple salt medi...

journal_title：Molecular and biochemical parasitology

authors： Tielens AG,van den Bergh SG

更新日期：1987-07-01 00:00:00

abstract：:Leishmania major promastigotes grown in late log phase were incubated with glucose as sole exogenous carbon source in the presence of 5% CO2 and the amounts of glucose consumed and of the major products formed--succinate, pyruvate, alanine, acetate, glycerol, and D-lactate--were measured as a function of pO2. Glucose ...

journal_title：Molecular and biochemical parasitology

authors： Keegan F,Blum JJ

更新日期：1990-03-01 00:00:00

abstract：:Parasitic infections are a widespread health problem and research of novel anthelmintic compounds is of the utmost importance. In this study we performed a virtual screening campaign by coupling ligand-based pharmacophore, homology modeling and molecular docking. The virtual screening campaign was conducted using a jo...

journal_title：Molecular and biochemical parasitology

authors： Stevanovic S,Marjanović DS,Trailović SM,Zdravković N,Perdih A,Nikolic K

更新日期：2021-01-07 00:00:00

abstract：:L-myo-Inositol 1-phosphate synthase (I-1-P synthase) catalyses the primary reaction for the synthesis of inositol in a variety of prokaryotes, eukaryotes and in the chloroplasts of algae and higher plants. Inositol is a precursor of essential macromolecules like membrane phospholipids, GPI anchor proteins and lipophos...

journal_title：Molecular and biochemical parasitology

authors： Lohia A,Hait NC,Majumder AL

更新日期：1999-01-05 00:00:00

abstract：:In order to clarify the interpretation of molecular karyotype polymorphisms in Leishmania, the three smallest chromosomes from five cloned strains of Leishmania infantum were identified by chromosome-specific anonymous DNA probes. The presence in the same clone of homologous chromosomes of a different size was demonst...

journal_title：Molecular and biochemical parasitology

authors： Blaineau C,Bastien P,Rioux JA,Roizès G,Pagès M

更新日期：1991-06-01 00:00:00

abstract：:In Trypanosoma brucei, the generation of knockout mutants is relatively easy compared to other organisms as transfection methods are well established. These methods have their limitations, however, when it comes to the generation of genome-wide libraries that require a minimum of several hundred thousand transformants...

journal_title：Molecular and biochemical parasitology

authors： Schumann G,Kangussu-Marcolino MM,Doiron N,Käser S,de Assis Burle-Caldas G,DaRocha WD,Teixeira SM,Roditi I

更新日期：2017-04-01 00:00:00

abstract：:Plasma membrane vesicles of Trypanosoma cruzi (PMVs) formed saturation binding isotherms with naive murine T lymphocytes. Parasite membrane attachment to the muscarinic cholinergic receptors of Lyt 2.2+T cells (suppressor cells) resulted in the synthesis of cGMP, attenuation of cAMP levels and in the secretion of pros...

journal_title：Molecular and biochemical parasitology

authors： Borda ES,Sterin-Borda LJ,Pascual JO,Gorelik G,Felix JC,von Kreuter BF,Santos-Buch CA

更新日期：1991-07-01 00:00:00

abstract：:The (Ca(2+)-Mg2+)ATPase activity in microsomes of Schistosoma mansoni is fully inhibited by vanadate (I50 = 2.5 microM). 45Ca2+ is accumulated within microsomal vesicles in an ATP-dependent process that is enhanced 5-fold in the presence of 40 mM phosphate. Accumulated 45Ca2+ is rapidly released by 5 microM of the Ca2...

journal_title：Molecular and biochemical parasitology

authors： Cunha VM,Meyer-Fernandes JR,Noël F

更新日期：1992-06-01 00:00:00

abstract：:cDNA clones encoding 473 amino acids of the knob-associated histidine-rich protein (PfHRPI) of Plasmodium falciparum clone FCR-3/A2 (Gambia) have been isolated and sequenced. Although a short region close to the amino terminus of the predicted sequence contains three blocks of six, seven or nine consecutive histidine ...

journal_title：Molecular and biochemical parasitology

authors： Ellis J,Irving DO,Wellems TE,Howard RJ,Cross GA

更新日期：1987-11-01 00:00:00

abstract：:Electron paramagnetic resonance spectrometry (EPR) techniques revealed a complex set of redox components in intact respiring cells of Tritrichomonas foetus, an aerotolerant anaerobic protozoon which lacks mitochondria. One of the two main systems of terminal respiration of this organism is the hydrogenosomal system, w...

journal_title：Molecular and biochemical parasitology

authors： Ohnishi T,Lloyd D,Lindmark DG,Müller M

更新日期：1980-10-01 00:00:00

abstract：:A strain of Plasmodium berghei (NK 65) was followed during syringe transmission in mice for over 120 passages after the last complete cycle, while the following parameters were monitored: (a) capacity to infect mosquitoes, inducing oocyst formation; (b) presence in the peripheral blood of morphologically identifiable ...

journal_title：Molecular and biochemical parasitology

authors： Birago C,Bucci A,Dore E,Frontali C,Zenobi P

更新日期：1982-07-01 00:00:00

abstract：:The 31P NMR spectrum of the adult tapeworm, Hymenolepis diminuta, at 37 degrees C during perfusion with physiological saline was composed of 10 peaks. Based on chemical shifts and analysis of worm extracts, the phosphorus components included glucose-6-phosphate, fructose-6-phosphate, phosphorylcholine, glycerophosphor...

journal_title：Molecular and biochemical parasitology

authors： Thompson SN,Platzer EG,Lee RW

更新日期：1987-01-02 00:00:00

abstract：:An axenic cultivation system was used to study the differentiation of Trypanosoma brucei bloodstream forms from long slender to short stumpy-like forms. Trypanosomes in the logarithmic phase are similar to long slender bloodstream forms freshly isolated from infected mice, differing only in the rate of oxygen uptake. ...

journal_title：Molecular and biochemical parasitology

authors： Hamm B,Schindler A,Mecke D,Duszenko M

更新日期：1990-04-01 00:00:00

abstract：:Intraerythrocytic Plasmodium falciparum digests vast amounts of hemoglobin within an acidic food vacuole (FV). Four homologous aspartic proteases participate in hemoglobin degradation within the FV. Plasmepsin (PM) I and II are thought to initiate degradation of the native hemoglobin molecule. PM IV and histo-aspartic...

journal_title：Molecular and biochemical parasitology

authors： Banerjee R,Francis SE,Goldberg DE

更新日期：2003-07-01 00:00:00

abstract：:Giardia lamblia is an early diverging eukaryote which causes gastrointestinal disease throughout the world. Different subgroups of Giardia have been defined based on several biochemical and genetic criteria. We have developed a method for stably introducing DNA into the nuclei of the parasite using puromycin acetyltra...

journal_title：Molecular and biochemical parasitology

authors： Singer SM,Yee J,Nash TE

更新日期：1998-04-01 00:00:00

abstract：:A membrane-bound sialidase (EC 3.2.1.18) was found in procyclic trypomastigotes of Trypanosoma brucei. The mammalian stage bloodstream form, however, displayed no sialidase activity. This sialidase is an integral surface protein, linked to the membrane via a glycosylphosphatidylinositol anchor. After osmotic lysis and...

journal_title：Molecular and biochemical parasitology

authors： Engstler M,Reuter G,Schauer R

更新日期：1992-08-01 00:00:00

abstract：:The extent of structural conservation of the Plasmodium falciparum sporozoite surface protein gene, STARP, recently characterized in the T9/96 clone, has been analyzed using the polymerase chain reaction. Results from Ivory Coast and Thai clones, field isolates originating from Brazil and Kenya and laboratory-maintain...

journal_title：Molecular and biochemical parasitology

authors： Fidock DA,Sallenave-Sales S,Sherwood JA,Gachihi GS,Ferreira-da-Cruz MF,Thomas AW,Druilhe P

更新日期：1994-10-01 00:00:00

abstract：:Kinetoplast DNA (kDNA), the mitochondrial DNA of trypanosomes, is a network of thousands of topologically interlocked DNA minicircles and about 50 maxicircles. In this study, we have analysed the kDNA molecules of 6 strains of Trypanosoma evansi from different geographical areas. 2 strains were found to be dyskinetopl...

journal_title：Molecular and biochemical parasitology

authors： Ou YC,Giroud C,Baltz T

更新日期：1991-05-01 00:00:00

abstract：:New phosphoproteins appear on the host erythrocyte membrane during Plasmodium berghei and P. chabaudi infection. Distinct proteins having similar properties and all distinguished by isoelectric points of less than 4.0 are identified. Associated with the erythrocyte membranes of P. berghei infected erythrocytes are two...

journal_title：Molecular and biochemical parasitology

authors： Wiser MF,Leible MB,Plitt B

更新日期：1988-01-01 00:00:00

abstract：:The enzyme pterin-4a-carbinolamine dehydratase (PCD) is important for the recycling of pterins within eukaryotic cells. A recombinant expression system for PCD from the apicomplexan parasite Toxoplasma gondii has been prepared, the protein purified and crystallised. Single crystal X-ray diffraction methods have produc...

journal_title：Molecular and biochemical parasitology

authors： Cameron S,Fyffe SA,Goldie S,Hunter WN

更新日期：2008-04-01 00:00:00

abstract：:A Mo7-derived Babesia bovis line stably transfected with the gfp-bsd gene was inoculated into two four to five months old calves, while two additional calves were inoculated with Mo7 parasites. Similar mild clinical signs were detected in all calves. B. bovis rap-1 was identified in the bloodstream by PCR four days po...

journal_title：Molecular and biochemical parasitology

authors： Suarez CE,Laughery JM,Schneider DA,Sondgeroth KS,McElwain TF

更新日期：2012-09-01 00:00:00

abstract：:Antigenic switching in Trypanosoma brucei can occur either by the production of a telomeric copy of a variant surface glycoprotein (VSG) gene through a gene conversion mechanism or by the nonduplicative activation of a telomeric VSG gene. The 5 VSG gene telomeric copy that is expressed in IsTaR 1 variant antigenic typ...

journal_title：Molecular and biochemical parasitology

authors： Aline RF Jr,Stuart K

更新日期：1985-06-01 00:00:00

abstract：:Trophozoites of Entamoeba invadens were able to ingest glucopolysaccharides and metabolize them. An activity capable of degrading these substrates was purified to apparent homogeneity. The enzyme was identified as beta-amylase (alpha-1,4-D-glucan maltohydrolase EC 3.2.1.2): It was active against glycogen, amylose and ...

journal_title：Molecular and biochemical parasitology

authors： Serafimova F,Franz A,Werries E

更新日期：1996-12-20 00:00:00

abstract：:Monoclonal antibodies designated 8G10/48 and 9E3/48 raised against mature asexual blood stages of Plasmodium falciparum inhibit parasite growth in vitro. Both antibodies bind to an epitope which includes the linear sequence Ser Thr Asn Ser and which is present in a cDNA clone from a P. falciparum expression library. T...

journal_title：Molecular and biochemical parasitology

authors： Epping RJ,Goldstone SD,Ingram LT,Upcroft JA,Ramasamy R,Cooper JA,Bushell GR,Geysen HM

更新日期：1988-02-01 00:00:00

abstract：:The merozoite of Plasmodium vivax possesses a high molecular mass surface protein called Pv-merozoite surface protein 1, PvMSP-1, which exhibits antigenic diversity among isolates. In this study, the extent of sequence variation in the polymorphic region and the flanking interspecies conserved blocks (ICBs) 5 and 6 of...

journal_title：Molecular and biochemical parasitology

authors： Putaporntip C,Jongwutiwes S,Tanabe K,Thaithong S

更新日期：1997-01-01 00:00:00

abstract：:Earlier studies in this laboratory had shown that the malarial parasite can synthesize heme de novo and inhibition of the pathway leads to death of the parasite. It has been proposed that the pathway for the biosynthesis of heme in Plasmodium falciparum is unique involving three different cellular compartments, namely...

journal_title：Molecular and biochemical parasitology

authors： Nagaraj VA,Arumugam R,Prasad D,Rangarajan PN,Padmanaban G

更新日期：2010-11-01 00:00:00

abstract：:We have compared a total of 44 recognition sites for 12 restriction endonucleases on the 20 kilobase pair maxi-circle of kinetoplast DNA from nine Trypanosoma brucei stocks, four which are known to be infective to man (tow 'gambiense' and two 'rhodesiense' variants). In addition to five polymorphic sites, these DNAs d...

journal_title：Molecular and biochemical parasitology

authors： Borst P,Fase-Fowler F,Gibson WC

更新日期：1981-06-01 00:00:00

abstract：:The cyclophilins are a large family of proteins implicated in folding, transport and regulation of other proteins and are potential drug targets in cancer and in some viral and parasitic infections. The functionality of cyclophilins appears to depend on peptidyl-prolyl cis-trans isomerase (foldase) and/or molecular ch...

journal_title：Molecular and biochemical parasitology

authors： Marín-Menéndez A,Monaghan P,Bell A

更新日期：2012-07-01 00:00:00

abstract：:Cytoadherence of infected erythrocytes is a hallmark of Plasmodium falciparum infection and a key determinant in the particular virulence of this species. Infected erythrocytes bind a variety of host receptors but certain adhesion traits are associated with more severe disease. A large, diverse protein family named P....

journal_title：Molecular and biochemical parasitology

authors： Kraemer SM,Gupta L,Smith JD

更新日期：2003-06-01 00:00:00