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A (Ca(2+)-Mg2+)ATPase from Schistosoma mansoni is coupled to an active transport of calcium.

Abstract：

:The (Ca(2+)-Mg2+)ATPase activity in microsomes of Schistosoma mansoni is fully inhibited by vanadate (I50 = 2.5 microM). 45Ca2+ is accumulated within microsomal vesicles in an ATP-dependent process that is enhanced 5-fold in the presence of 40 mM phosphate. Accumulated 45Ca2+ is rapidly released by 5 microM of the Ca2+ ionophore A23187 (t1/2 less than or equal to 6 s). (Ca(2+)-Mg2+)ATPase activity and Ca2+ uptake share the same subcellular distribution pattern and similar Ca2+ sensitivities (K0.5 = 0.39 microM and 0.15 microM, respectively). The substrate selectivity is high for both ATPase activity and Ca2+ transport. These results indicate the presence of an active transport of Ca2+ coupled to the (Ca(2+)-Mg2+)ATPase activity previously described in this parasite. A plasma membrane localization and physiological role in calcium homeostasis are suggested.

journal_name

Mol Biochem Parasitol

journal_title

Molecular and biochemical parasitology

authors

Cunha VM,Meyer-Fernandes JR,Noël F

doi

10.1016/0166-6851(92)90049-p

subject

Has Abstract

pub_date

1992-06-01 00:00:00

pages

167-73

issue

2

eissn

0166-6851

issn

1872-9428

pii

0166-6851(92)90049-P

journal_volume

52

pub_type

杂志文章

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