Abstract:
:Giardia lamblia depends on glycolysis to obtain ATP, highlighting the suitability of glycolytic enzymes as targets for drug design. We studied triosephosphate isomerase from G. lamblia (GlTIM) as a potential species-specific drug target. Cysteine-reactive agents were used as probes, in order to test those regions near to cysteine residues as targets to perturb enzyme structure and activity. Methyl methanethiosulfonate (MMTS) derivatized three of the five Cys per subunit of dimeric GlTIM and induced 50% of inactivation. The 2-carboxyethyl methanethiosulfonate (MTSCE) modified four Cys and induced 97% of inactivation. Inactivation by MMTS or MTSCE did not affect secondary structure, nor induce dimer dissociation; however, Cys modification decreased thermal stability of enzyme. Inactivation and dissociation of the dimer to stable monomers were reached when four Cys were derivatized by 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB). The effects of DTNB were completely abolished when GlTIM was first treated with MMTS. The effect of thiol reagents on human TIM was also assayed; it is 180-fold less sensitive than GlTIM. Collectively, the data illustrate GlTIM as a good target for drug design.
journal_name
Mol Biochem Parasitoljournal_title
Molecular and biochemical parasitologyauthors
Enriquez-Flores S,Rodriguez-Romero A,Hernandez-Alcantara G,De la Mora-De la Mora I,Gutierrez-Castrellon P,Carvajal K,Lopez-Velazquez G,Reyes-Vivas Hdoi
10.1016/j.molbiopara.2007.10.013subject
Has Abstractpub_date
2008-02-01 00:00:00pages
179-86issue
2eissn
0166-6851issn
1872-9428pii
S0166-6851(07)00306-4journal_volume
157pub_type
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journal_title:Molecular and biochemical parasitology
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journal_title:Molecular and biochemical parasitology
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