Molecular characterization of the alpha-subunit of Trichomonas vaginalis hydrogenosomal succinyl CoA synthetase.


:The anaerobic, parasitic protist, Trichomonas vaginalis, is characterized by the absence of mitochondria and the presence of double membrane bound organelles called hydrogenosomes. Succinyl-coenzyme A synthetase is a hydrogenosomal enzyme which catalyzes the formation of ATP via substrate-level phosphorylation. We have characterized genes encoding the alpha subunit of the hydrogenosomal protein succinyl-coenzyme A synthetase (SCS). The alpha-SCS of T. vaginalis is encoded by a multigene family composed of 3 similar genes that do not appear allelic. These 3 alpha-SCS genes encode a protein with a calculated molecular mass of approximately 32.5 kDa that has > 50% identity (> 70% similarity with alpha-SCSs from Escherichia coli, Thermus flavus, and rat liver mitochondria. Antibodies raised against recombinant T vaginalis alpha-SCS expressed in bacteria were used to isolate alpha-SCS proteins from purified hydrogenosomes. These proteins partition into the soluble fraction of hydrogenosomes treated with sodium carbonate at high pH, consistent with a matrix localization in the organelle. Amino-terminal sequencing of purified alpha-SCS proteins shows that mature proteins lack a short, leader sequence of 9 amino acids. These amino terminal sequences which are cleaved from T. vaginalis alpha-SCSs are similar to each other and to all other leader sequences identifed on hydrogenosomal proteins.


Mol Biochem Parasitol


Lahti CJ,Bradley PJ,Johnson PJ




Has Abstract


1994-08-01 00:00:00














  • A high level of mixed Trypanosoma brucei infections in tsetse flies detected by three hypervariable minisatellites.

    abstract::The issue of whether genetic exchange occurs at a significant frequency in natural populations of Trypanosoma brucei is controversial and one of the arguments against a high frequency has been the apparent lack of host infections with mixtures of trypanosome genotypes. Three minisatellite markers (MS42, CRAM, 292) wit...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: MacLeod A,Turner CM,Tait A

    更新日期:1999-08-20 00:00:00

  • The nucleotide sequence of the variable region in Trypanosoma brucei completes the sequence analysis of the maxicircle component of mitochondrial kinetoplast DNA.

    abstract::The nucleotide sequence of two non-contiguous DNA fragments of 4.0 and 2.2 kb, respectively, of the kinetoplast maxicircle of Trypanosoma brucei brucei EATRO strain 427 has been determined, completing the sequence analysis of the so-called variable region (see also de Vries et al., 1988, Mol. Biochem. Parasitol. 27, 7...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Sloof P,de Haan A,Eier W,van Iersel M,Boel E,van Steeg H,Benne R

    更新日期:1992-12-01 00:00:00

  • Upstream regulatory elements controlling expression of the Entamoeba histolytica lectin.

    abstract::Entamoeba histolytica genomic organization and putative promoter elements appear to be distinct from both metazoan and better characterized protozoan organisms. The recent development of DNA-mediated transfection for E. histolytica enabled characterization of cis-acting promoter elements required for gene expression. ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Purdy JE,Pho LT,Mann BJ,Petri WA Jr

    更新日期:1996-06-01 00:00:00

  • Down-regulation of flavin reductase and alcohol dehydrogenase-1 (ADH1) in metronidazole-resistant isolates of Trichomonas vaginalis.

    abstract::The microaerophilic parasite Trichomonas vaginalis is a causative agent of painful vaginitis or urethritis, termed trichomoniasis, and can also cause preterm delivery or stillbirth. Treatment of trichomoniasis is almost exclusively based on the nitroimidazole drugs metronidazole and tinidazole. Metronidazole resistanc...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Leitsch D,Drinić M,Kolarich D,Duchêne M

    更新日期:2012-06-01 00:00:00

  • Ecdysteroid excretion by adult Hymenolepis diminuta in vitro.

    abstract::Both patent and prepatent adult Hymenolepis diminuta excreted 20-hydroxyecdysone into the culture medium when maintained in vitro. Patent worms also excreted ecdysone and comparatively large quantities of unidentified immunoreactive material of a relatively apolar nature. This latter material was shown to be depleted ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Mercer JG,Munn AE,Arme C,Rees HH

    更新日期:1987-12-01 00:00:00

  • A (Ca(2+)-Mg2+)ATPase from Schistosoma mansoni is coupled to an active transport of calcium.

    abstract::The (Ca(2+)-Mg2+)ATPase activity in microsomes of Schistosoma mansoni is fully inhibited by vanadate (I50 = 2.5 microM). 45Ca2+ is accumulated within microsomal vesicles in an ATP-dependent process that is enhanced 5-fold in the presence of 40 mM phosphate. Accumulated 45Ca2+ is rapidly released by 5 microM of the Ca2...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Cunha VM,Meyer-Fernandes JR,Noël F

    更新日期:1992-06-01 00:00:00

  • A role for tertiary structure in the generation of antigenic diversity and molecular association of the Tams1 polypeptide in Theileria annulata.

    abstract::The major merozoite-piroplasm surface antigen (mMPSA) of Theileria annulata, Tams1, is known to be antigenically diverse. The possession of variable N-linked glycosylation sites and removal of monoclonal antibody 5E1 reactivity by mild periodate treatment suggested, previously, that divergent epitopes may be conferred...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Katzer F,McKellar S,Ferguson MA,d'Oliveira C,Shiels BR

    更新日期:2002-06-01 00:00:00

  • A tubulin-related 55 kilodalton surface antigen recognized by different Trypanosoma cruzi stage-specific monoclonal antibodies from infected mice.

    abstract::Thirteen monoclonal antibodies (MAbs) specific for the membrane of live Trypanosoma cruzi have been obtained from BALB/c infected mice. Most of them had greater avidity for intact than for disrupted parasites. According to the staining by indirect immunofluorescence of the different live developmental stages of the pa...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Alcina A,Fresno M

    更新日期:1988-06-01 00:00:00

  • Genetic and antigenic characterization of Babesia bovis merozoite spherical body protein Bb-1.

    abstract::A Babesia bovis merozoite protein, Bb-1, was localized by immunoelectron microscopy to an apical organelle known as the spherical body. This unique structure appears to be analogous to dense granules of other apicomplexan protozoa. Similar to previously described dense granule proteins of Plasmodium spp., Bb-1 is secr...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hines SA,Palmer GH,Brown WC,McElwain TF,Suarez CE,Vidotto O,Rice-Ficht AC

    更新日期:1995-02-01 00:00:00

  • Structural features affecting variant surface glycoprotein expression in Trypanosoma brucei.

    abstract::The glycosylphosphatidylinositol (GPI)-anchored variant surface glycoprotein (VSG) of Trypanosoma brucei is the most abundant GPI-anchored protein expressed on any cell, and is an essential virulence factor. To determine what structural features affect efficient expression of VSG, we made a series of mutations in two ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Wang J,Böhme U,Cross GA

    更新日期:2003-05-01 00:00:00

  • The mitochondrial phosphate carrier TbMCP11 is essential for mitochondrial function in the procyclic form of Trypanosoma brucei.

    abstract::Conserved amongst all eukaryotes is a family of mitochondrial carrier proteins (SLC25A) responsible for the import of various solutes across the inner mitochondrial membrane. We previously reported that the human parasite Trypanosoma brucei possesses 26 SLC25A proteins (TbMCPs) amongst which two, TbMCP11 and TbMCP8, w...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Gao F,Voncken F,Colasante C

    更新日期:2020-05-01 00:00:00

  • Isolation and characterization of infective and non-infective clones of Leishmania tropica.

    abstract::Cloning by limit dilution of an isolate of Leishmania tropica (LRC-L137) that is infective for mice resulted in 7 stable clones, only one of which was infective in BALB/c mice. Three of the non-infective clones that were examined for survival in BALB/c macrophages in vitro seemed to be killed more readily, suggesting ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Handman E,Hocking RE,Mitchell GF,Spithill TW

    更新日期:1983-02-01 00:00:00

  • A developmentally regulated cysteine protease gene family in Haemonchus contortus.

    abstract::The nucleotide sequence of a gene encoding a 35-kDa thiol protease of the parasitic nematode Haemonchus contortus has been determined. The gene, designated AC-2, shares 97% nucleotide sequence identity and 98% amino acid identity with previously characterized AC-1 cDNAs encoding the thiol protease. The AC-2 gene spans...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Pratt D,Cox GN,Milhausen MJ,Boisvenue RJ

    更新日期:1990-12-01 00:00:00

  • Assessment of pfmdr 1 gene copy number by tandem competitive polymerase chain reaction.

    abstract::The pfmdr 1 gene encodes a Plasmodium falciparum homologue of the human P-glycoprotein expressed on the surface of the parasite food vacuole. Variation in copy number and specific codon mutations of pfmdr 1 have been implicated in the development of parasite resistance to antimalarial drugs. We describe here the techn...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Price R,Robinson G,Brockman A,Cowman A,Krishna S

    更新日期:1997-04-01 00:00:00

  • Heat shock and stress response in Giardia lamblia.

    abstract::The heat shock response was studied as a model for control of gene expression and protein synthesis in Giardia lamblia. Cultured trophozoites were metabolically labelled with [35S]methionine, and proteins were analysed by SDS-polyacrylamide gel electrophoresis and fluorography. A temperature shift from 37 degrees C to...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Lindley TA,Chakraborty PR,Edlind TD

    更新日期:1988-03-01 00:00:00

  • A diamidine-resistant Trypanosoma equiperdum clone contains a P2 purine transporter with reduced substrate affinity.

    abstract::Following the demonstration that the transport of melaminophenyl arsenical drugs in Trypanosoma brucei is dependent upon an unusual adenosine nucleoside transporter (Carter and Fairlamb, Nature 361 (1993) 173-175) we have investigated adenosine transport in the related parasite Trypanosoma equiperdum (Botat1.1) and a ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Barrett MP,Zhang ZQ,Denise H,Giroud C,Baltz T

    更新日期:1995-07-01 00:00:00

  • The Leishmania mexicana proteasome.

    abstract::As a start to understanding the importance of intracellular proteolysis in the protozoon Leishmania mexicana, the parasite proteasome has been purified and characterised. The L. mexicana proteasome is similar to proteasomes from other eukaryotes. It is soluble, and the 20S form has a mass of around 670 kDa, composed o...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Robertson CD

    更新日期:1999-09-20 00:00:00

  • SYBR, TaqMan, or both: highly sensitive, non-invasive detection of Cardicola blood fluke species in Southern Bluefin Tuna (Thunnus maccoyii).

    abstract::Three species of blood fluke from the genus Cardicola are known to parasitize and cause disease in Bluefin Tunas--C. forsteri, C. orientalis, and C. opisthorchis. Although initially believed to be separated by geography and host specificity, recent identification of at least two Cardicola spp. concurrently present wit...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Polinski M,Hamilton DB,Nowak B,Bridle A

    更新日期:2013-09-01 00:00:00

  • Molecules on the surface of the Plasmodium falciparum infected erythrocyte and their role in malaria pathogenesis and immune evasion.

    abstract::The surface of the erythrocyte undergoes a number of modifications during infection by Plasmodium falciparum. These modifications are critical for pathogenesis of severe disease and the acquisition of host immunity through their role in interactions between the host and the parasite and in antigenic variation. Our kno...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Craig A,Scherf A

    更新日期:2001-07-01 00:00:00

  • Structural analysis of the acetaldehyde dehydrogenase activity of Entamoeba histolytica alcohol dehydrogenase 2 (EhADH2), a member of the ADHE enzyme family.

    abstract::The ADHE family of enzymes are bifunctional acetaldehyde dehydrogenase (ALDH)/alcohol dehydrogenase (ADH) enzymes that probably arose from the fusion of genes encoding separate ALDH and ADH enzymes. Here we have used the Entamoeba histolytica alcohol dehydrogenase 2 (EhADH2) enzyme as a prototype to analyze the struct...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Chen M,Li E,Stanley SL Jr

    更新日期:2004-10-01 00:00:00

  • Leupeptin alters the proteolytic processing of P126, the major parasitophorous vacuole antigen of Plasmodium falciparum.

    abstract::Among several protease inhibitors tested, only leupeptin was found to modify qualitatively the processing of P126, a major antigen of the parasitophorous vacuole of Plasmodium falciparum, and to inhibit the release of merozoites. Whereas P126 is normally processed upon merozoite release into 2 polypeptides of 50 and 7...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Debrabant A,Delplace P

    更新日期:1989-03-01 00:00:00

  • Comparative biochemical and functional properties of two leucine aminopeptidases of Clonorchis sinensis.

    abstract::Leucine aminopeptidases (LAP; EC are a group of metalloexopeptidases, which catalyze the sequential removal of leucine amino acids from the N-termini of the polypeptides or proteins. In this study, we identified two novel genes that encode LAPs of Clonorchis sinensis (CsLAP1 and CsLAP2) and characterized the...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kang JM,Ju HL,Ju JW,Sohn WM,Kim TS,Bahk YY,Hong SJ,Na BK

    更新日期:2012-03-01 00:00:00

  • Genetic variability and evolution of the Schistosoma genome analysed by using random amplified polymorphic DNA markers.

    abstract::The usefulness of random amplified polymorphic DNA markers (RAPD) was assayed in an attempt to discriminate among species, strains and individuals within the genus Schistosoma. Depending on the species, 40-50 arbitrary decamer oligonucleotides were used as primers to amplify total DNA by the polymerase chain reaction ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Barral V,This P,Imbert-Establet D,Combes C,Delseny M

    更新日期:1993-06-01 00:00:00

  • Microsatellite polymorphism in the sexually transmitted human pathogen Trichomonas vaginalis indicates a genetically diverse parasite.

    abstract::Given the growing appreciation of serious health sequelae from widespread Trichomonas vaginalis infection, new tools are needed to study the parasite's genetic diversity. To this end we have identified and characterized a panel of 21 microsatellites and six single-copy genes from the T. vaginalis genome, using seven l...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Conrad M,Zubacova Z,Dunn LA,Upcroft J,Sullivan SA,Tachezy J,Carlton JM

    更新日期:2011-01-01 00:00:00

  • Molecular characterisation of an expressed sequence tag locus of Toxoplasma gondii encoding the micronemal protein MIC2.

    abstract::The expressed sequence tag (EST) dataset of Toxoplasma gondii provides a wealth of information towards gene discovery. The complete cDNA and genomic sequence of EST tgc050 locus shows that it contains five copies of the conserved thrombospondin (TSP)-like motif present in a number of molecules with adhesive properties...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Wan KL,Carruthers VB,Sibley LD,Ajioka JW

    更新日期:1997-02-01 00:00:00

  • cDNA expressed sequence tags of Trypanosoma brucei rhodesiense provide new insights into the biology of the parasite.

    abstract::A total of 518 expressed sequence tags (ESTs) have been generated from clones randomly selected from a cDNA library and a spliced leader sub-library of a Trypanosoma brucei rhodesiense bloodstream clone. 205 (39%) of the clones were identified based on matches to 113 unique genes in the public databases. Of these, 71 ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: el-Sayed NM,Alarcon CM,Beck JC,Sheffield VC,Donelson JE

    更新日期:1995-07-01 00:00:00

  • The influence of temperature and serum deprivation on the synthesis of heat-shock proteins and alpha and beta tubulin in promastigotes of Leishmania major.

    abstract::We have examined changes in the relative synthesis of individual proteins in promastigotes of Leishmania major subjected to decreasing serum levels in vitro. We observed increases in the relative synthesis of the putative heat-shock proteins of 82 and 70 kDa and of proteins of 79 and 41 kDa but decreases in the synthe...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Toye P,Remold H

    更新日期:1989-06-01 00:00:00

  • The presence of galactofuranose and ribose units in asparagine-linked oligosaccharides of the digenetic trypanosomatid Endotrypanum schaudinni.

    abstract::It was found that the digenetic trypanosomatid Endotrypanum schaudinni transferred Man7GlcNAc2 in protein N-glycosylation. Endo-beta-N-acetylglucosaminidase H-sensitive oligosaccharides were identified as Man7GlcNAc2, Man6GlcNAc2, Rib1Man6GlcNAc2 and/or Gal1Man6GlcNAc2, Man5GlcNAc containing two galactose or ribose un...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Merello S,Xavier MT,Parodi AJ

    更新日期:1995-01-01 00:00:00

  • The Kennedy phospholipid biosynthesis pathways are refractory to genetic disruption in Plasmodium berghei and therefore appear essential in blood stages.

    abstract::Phosphatidylcholine (PC) and phosphatidylethanolamine (PE) are the main membrane phospholipids (PLs) of Plasmodium parasites and can be generated by the de novo (Kennedy) CDP-choline and CDP-ethanolamine pathways and by the CDP-diacylglycerol dependent pathway. The Kennedy pathways initiate from exogenous choline and ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Déchamps S,Wengelnik K,Berry-Sterkers L,Cerdan R,Vial HJ,Gannoun-Zaki L

    更新日期:2010-10-01 00:00:00

  • Anopheles stephensi salivary glands bear receptors for region I of the circumsporozoite protein of Plasmodium falciparum.

    abstract::In the mosquito, Plasmodium sporozoites rupture from oocysts found on the midgut wall, circulate in the hemolymph and invade salivary glands where they wait to be injected into a vertebrate host during a bloodmeal. The mechanisms by which sporozoites specifically attach to and invade salivary glands are not known but ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Sidjanski SP,Vanderberg JP,Sinnis P

    更新日期:1997-12-01 00:00:00