Molecular characterization of the alpha-subunit of Trichomonas vaginalis hydrogenosomal succinyl CoA synthetase.

abstract：:Incorporation of 32Pi into organic phosphate by mitochondria of Cotugnia digonopora was supported maximally by malate. Fumarate and succinate induced lower but significant production of ATP. Pyruvate, alpha-ketoglutarate and oxalacetate proved to be poor substrates and citrate and isocitrate had no effect. A net phosp...

journal_title：Molecular and biochemical parasitology

authors： Pampori NA,Singh G,Srivastava VM

更新日期：1984-04-01 00:00:00

abstract：:Many of the currently available nematicides used in nematode control are hazardous to the user, environment and beneficial non-target organisms. Therefore the need to develop alternative methods for nematode control such as the development of nematode-resistant crops through RNA-mediated interference (RNAi) holds grea...

journal_title：Molecular and biochemical parasitology

authors： Joseph S,Gheysen G,Subramaniam K

更新日期：2012-11-01 00:00:00

abstract：:A 230 000 molecular weight (MW) Plasmodium yoelii protein, a 250 000 MW P. chabaudi protein and a 195 000 MW P. falciparum protein, identified using monoclonal antibodies, have similar characteristics, and have been implicated as protective antigens. In this study the serological relationship between these proteins wa...

journal_title：Molecular and biochemical parasitology

authors： Holder AA,Freeman RR,Newbold CI

更新日期：1983-11-01 00:00:00

abstract：:The surface of the erythrocyte undergoes a number of modifications during infection by Plasmodium falciparum. These modifications are critical for pathogenesis of severe disease and the acquisition of host immunity through their role in interactions between the host and the parasite and in antigenic variation. Our kno...

journal_title：Molecular and biochemical parasitology

authors： Craig A,Scherf A

更新日期：2001-07-01 00:00:00

abstract：:Babesia bovis, an intraerythrocytic, protozoal parasite of cattle, undergoes clonal antigenic variation (Allred DR, Cinque RM, Lane TJ, Ahrens KP. Infect Immun 1994;62:91-98). This ability could provide a mechanism by which the parasite escapes host immune defenses to establish chronic infection. Previous work identif...

journal_title：Molecular and biochemical parasitology

authors： O'Connor RM,Lane TJ,Stroup SE,Allred DR

更新日期：1997-11-01 00:00:00

abstract：:Contrary to previous reports in the literature, bloodstream forms of the haemoflagellate protozoan Trypanosoma brucei brucei are not deficient in their ability to metabolize hydrogen peroxide, although they either lack or only possess the normal enzymes for H2O2 detoxification, catalase (EC 1.11.1.6) and glutathione p...

journal_title：Molecular and biochemical parasitology

authors： Penketh PG,Klein RA

更新日期：1986-08-01 00:00:00

abstract：:The aim of this study was to investigate the occurrence, distribution and immunochemical properties of antibody-defined carbohydrate epitopes in neutral glycolipid fractions of Schistosoma mansoni eggs, cercariae and adults. The amount of extractable, antigenic, neutral glycolipids was lowest in adult worms, increasin...

journal_title：Molecular and biochemical parasitology

authors： Wuhrer M,Dennis RD,Doenhoff MJ,Bickle Q,Lochnit G,Geyer R

更新日期：1999-10-15 00:00:00

abstract：:The in vitro-released 'excretory/secretory' (ES) and somatic antigens of the third stage (infective) larva of Anisakis simplex were characterised by radioiodination, immunoprecipitation, and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Oral infection with the parasite elicited a substantial IgG antibody...

journal_title：Molecular and biochemical parasitology

authors： Kennedy MW,Tierney J,Ye P,McMonagle FA,McIntosh A,McLaughlin D,Smith JW

更新日期：1988-10-01 00:00:00

abstract：:Trypanosoma brucei is a unicellular parasite that is transmitted from one mammalian host to the next by tsetse flies. The expression of many trypanosome genes is regulated during the life cycle but there is no evidence for developmental control of transcription by RNA polymerase II. T. brucei expresses at least two he...

journal_title：Molecular and biochemical parasitology

authors： Hotz HR,Lorenz P,Fischer R,Krieger S,Clayton C

更新日期：1995-12-01 00:00:00

abstract：:Schistosoma mansoni p14 gene encodes an eggshell precursor that is expressed only in vitelline cells of mature female worms in response to a male stimulus. The upstream region of the p14 gene contains several potential cis-acting regulatory sequences. We used the upstream region of the p14 gene as bait in a yeast-one-...

journal_title：Molecular and biochemical parasitology

authors： Fantappie MR,Osman A,Niles EG,LoVerde PT

更新日期：2000-10-01 00:00:00

abstract：:Malaria parasites export 'a secretome' of hundreds of proteins, including major virulence determinants, from their endoplasmic reticulum (ER), past the parasite plasma and vacuolar membranes to the host erythrocyte. The export mechanism is high affinity (nanomolar) binding of a host (cell) targeting (HT) motif RxLxE/D...

journal_title：Molecular and biochemical parasitology

authors： Bhattacharjee S,Speicher KD,Stahelin RV,Speicher DW,Haldar K

更新日期：2012-10-01 00:00:00

abstract：:A poly-zinc finger protein, designated PZFP1 was identified in Trypanosoma cruzi for the first time. The protein has 191 amino acids, contains seven motifs Cys(X)(2)Cys(X)(4)His(X)(4)Cys. A recombinant PZFP1 was generated in E. coli and the expected 21kDa polypeptide co-purified with two other inducible products of ab...

journal_title：Molecular and biochemical parasitology

authors： Espinosa JM,Portal D,Lobo GS,Pereira CA,Alonso GD,Gómez EB,Lan GH,Pomar RV,Flawiá MM,Torres HN

更新日期：2003-09-01 00:00:00

abstract：:Helicases are ubiquitous essential enzymes which have significant role in the nucleic acid metabolism. Using in silico approaches in the recent past we have identified a number of helicases in the Plasmodium falciparum genome. In the present study we report purification and detailed characterization of a novel helicas...

journal_title：Molecular and biochemical parasitology

authors： Mehta J,Tuteja R

更新日期：2011-03-01 00:00:00

abstract：:gamma-Glutamyl transpeptidase, which is of central importance in the degradation of glutathione, was purified from Ascaris suum to apparent homogeneity. The enzyme was found to be a predominantly membrane-bound protein and was solubilized by Triton X-100. The purified enzyme, which exhibits a specific activity of 1009...

journal_title：Molecular and biochemical parasitology

authors： Hussein AS,Walter RD

更新日期：1996-04-01 00:00:00

abstract：:An immunodominant 72-kDa surface glycoprotein (Gp72) of Trypanosoma cruzi is involved in adhesion of the flagellum to the cell body (Cooper, R, Ribeiro de Jesus, A and Cross, G.A.M (1993) J. Cell Biol. 122, 149-156). We have characterized a gene, flagellum-adhesion glycoprotein genel (fla1), from Trypanosoma brucei th...

journal_title：Molecular and biochemical parasitology

authors： Nozaki T,Haynes PA,Cross GA

更新日期：1996-11-25 00:00:00

abstract：:Genetic transformation of malaria parasites has been limited by the number of selectable markers available. For the rodent malaria parasite, Plasmodium berghei, only a single selection marker has been at hand, utilising the dihydrofolate reductase-thymidylate synthase gene from either P. berghei or Toxoplasma gondii t...

journal_title：Molecular and biochemical parasitology

authors： de Koning-Ward TF,Fidock DA,Thathy V,Menard R,van Spaendonk RM,Waters AP,Janse CJ

更新日期：2000-03-05 00:00:00

abstract：:Two NAD(H)-dependent malate dehydrogenase (MDH) isoenzymes were detected in Phytomonas isolated from the lactiferous tubes of Euphorbia characias. The total specific activity in crude extracts using oxaloacetate as substrate was 3.3 U mg-1 of protein. The two isoenzymes had isoelectric points of 6.0 and 7.2, respectiv...

journal_title：Molecular and biochemical parasitology

authors： Uttaro AD,Opperdoes FR

更新日期：1997-10-01 00:00:00

abstract：:We have successfully transfected Entamoeba histolytica trophozoites with constructs containing ferredoxin sequences fused to the reporter gene luciferase. We have determined the conditions and parameters necessary to maximise transient luciferase expression in our system. Our optimal construct gave values of 268 x 10(...

journal_title：Molecular and biochemical parasitology

authors： Gilchrist CA,Streets HL,Ackers JP,Hall R

更新日期：1995-10-01 00:00:00

abstract：:As a start to understanding the importance of intracellular proteolysis in the protozoon Leishmania mexicana, the parasite proteasome has been purified and characterised. The L. mexicana proteasome is similar to proteasomes from other eukaryotes. It is soluble, and the 20S form has a mass of around 670 kDa, composed o...

journal_title：Molecular and biochemical parasitology

authors： Robertson CD

更新日期：1999-09-20 00:00:00

abstract：:The secretory organelles of Toxoplasma gondii orchestrate invasion of the host cell and establish the parasitophorous vacuole. Although much has been learned about the roles played by these organelles in invasion by the tachyzoite stage, little is known about the contents or functions of these organelles during bradyz...

journal_title：Molecular and biochemical parasitology

authors： Schwarz JA,Fouts AE,Cummings CA,Ferguson DJ,Boothroyd JC

更新日期：2005-12-01 00:00:00

abstract：:The steady-state level of the hsp70 mRNAs of Trypanosoma cruzi cultured at different temperatures and growth conditions has been analyzed by Northern blotting. We show that only one size class of hsp70 mRNA, of about 2.2 kb, is transcribed from the hsp70 cluster and that its transcription is constitutive at 28 degrees...

journal_title：Molecular and biochemical parasitology

authors： Requena JM,Jimenez-Ruiz A,Soto M,Assiego R,Santarén JF,Lopez MC,Patarroyo ME,Alonso C

更新日期：1992-07-01 00:00:00

abstract：:Both the alpha- and beta-tubulin genes of Leishmania enriettii are encoded by mRNA of 2.0-2.2 kb in length. We have shown previously that the alpha- and beta-tubulin genes are arranged in separate, tandem repeats of 2 and 4 kb, respectively, and now report the mapping of mature mRNA onto these cloned genes. Here we sh...

journal_title：Molecular and biochemical parasitology

authors： Landfear SM,Wirth DF

更新日期：1985-04-01 00:00:00

abstract：:Procyclic Trypanosoma brucei brucei strain 366D is susceptible to DL-alpha-difluoromethylornithine (DFMO) with an in vitro ED50 value of 225 microM. A mutant of the procyclic strain resistant to 20 mM of DFMO was isolated by serial in vitro passages of the organisms in increasing concentrations of the drug. Drug resis...

journal_title：Molecular and biochemical parasitology

authors： Phillips MA,Wang CC

更新日期：1987-01-02 00:00:00

abstract：:The ATP-binding cassette (ABC) transporters are one of the largest evolutionarily conserved families of proteins. They are characterized by the presence of nucleotide-binding domains (NBDs), which are highly conserved among organisms. In the present study, we used human and protozoan ABC sequences, and ATP-binding con...

journal_title：Molecular and biochemical parasitology

authors： Sauvage V,Millot JM,Aubert D,Visneux V,Marle-Plistat M,Pinon JM,Villena I

更新日期：2006-06-01 00:00:00

abstract：:Trichomonas vaginalis is a parasitic protozoan of both medical and biological relevance. Transcriptional studies in this organism have focused mainly on type II pol promoters, whereas the elements necessary for transcription by polI or polIII have not been investigated. Here, with the aid of a transient transcription ...

journal_title：Molecular and biochemical parasitology

authors： Franco B,Hernández R,López-Villaseñor I

更新日期：2012-09-01 00:00:00

abstract：:Actin-related proteins (Arps) constitute a family of eukaryotic cytoskeletal proteins involved in such diverse events as cell motility, cytokinesis, vesicle transport, and chromatin remodelling. Previously, in a study of Plasmodium berghei gene expression in ookinetes and oocysts, we detected stage-specific increased ...

journal_title：Molecular and biochemical parasitology

authors： Siden-Kiamos I,Schüler H,Liakopoulos D,Louis C

更新日期：2010-10-01 00:00:00

abstract：:The cyclophilins are a large family of proteins implicated in folding, transport and regulation of other proteins and are potential drug targets in cancer and in some viral and parasitic infections. The functionality of cyclophilins appears to depend on peptidyl-prolyl cis-trans isomerase (foldase) and/or molecular ch...

journal_title：Molecular and biochemical parasitology

authors： Marín-Menéndez A,Monaghan P,Bell A

更新日期：2012-07-01 00:00:00

abstract：:AF3 (AVPGVLRFamide) is an endogenous RFamide-like peptide isolated from the parasitic nematode Ascaris suum. It has a potent and long lasting excitatory effect in A. suum and Ascaridia galli. This is mediated by a mechanism independent of the nicotinic-like acetylcholine (ACh) receptor, which mediates excitatory trans...

journal_title：Molecular and biochemical parasitology

authors： Trim N,Brooman JE,Holden-Dye L,Walker RJ

更新日期：1998-06-01 00:00:00

abstract：:We have shown that the Leishmania major transfection vector pR-NEO (or derivatives thereof) can be introduced and stably maintained in four species complexes of pathogenic Leishmania (L. tropica, L. mexicana, L. donovani, L. braziliensis), and the genera Endotrypanum and Crithidia; transfection of Trypanosoma cruzi or...

journal_title：Molecular and biochemical parasitology

authors： Coburn CM,Otteman KM,McNeely T,Turco SJ,Beverley SM

更新日期：1991-05-01 00:00:00

abstract：:Species of Centrorhynchus (Polymorphida: Centrorhynchidae) commonly parasitize various falconiform and strigiform birds worldwide. In the present study, the complete mitochondrial (mt) genome sequences of Centrorhynchus clitorideus was sequenced and annotated for the first time based on specimens collected from the li...

journal_title：Molecular and biochemical parasitology

authors： Muhammad N,Suleman,Khan MS,Li L,Zhao Q,Ullah H,Zhu XQ,Ma J

更新日期：2020-05-01 00:00:00