Structure of mRNA encoded by tubulin genes in Leishmania enriettii.

abstract：:Variation of Babesia bovis merozoite surface antigens occurs among geographic strains of the parasite. In this and a concurrent report, we investigate this variation at the gene and protein level. Using a monoclonal antibody (mAb 23/70.174), B. bovis gene sequences were identified that encoded a surface epitope of a 4...

journal_title：Molecular and biochemical parasitology

authors： Jasmer DP,Reduker DW,Hines SA,Perryman LE,McGuire TC

更新日期：1992-10-01 00:00:00

abstract：:Phosphatidylcholine (PC) is the most abundant phospholipid in the membranes of the human parasite Leishmania. It is synthesized via two metabolic routes, the de novo pathway that starts with the uptake of choline, and the threefold methylation of phosphatidylethanolamine. Choline was shown to be dispensable for Leishm...

journal_title：Molecular and biochemical parasitology

authors： Bibis SS,Dahlstrom K,Zhu T,Zufferey R

更新日期：2014-09-01 00:00:00

abstract：:Two cloned chromosomal segments of the hemoprotozoan Babesa bovis bearing genes from the polymorphic locus designated BabR were inserted into plasmid vector YCp19, cloned in Escherichia coli and then introduced into Saccharomyces cerevisiae. Both genes were transcribed producing poly(A+) RNAs. In at least one case, th...

journal_title：Molecular and biochemical parasitology

authors： Kemp DJ,Easton KE,Cowman AF

更新日期：1984-05-01 00:00:00

abstract：:One of the candidate schistosome antigens for the development of a circulating antigen detection diagnostic assay is the circulating cathodic antigen (CCA). Detection of CCA in urine provides a non-invasive assay with high sensitivity. Previously we reported that CCA is secreted in patients' urine as a small molecular...

journal_title：Molecular and biochemical parasitology

authors： Abdeen HH,Attallah AF,Mansour MM,Harrison RA

更新日期：1999-06-25 00:00:00

abstract：:[14C]Artemisinin was taken up by Plasmodium falciparum in culture and concentrated in hemozoin. In vitro, hemin and artemisinin were found to undergo a chemical reaction forming two major products which were isolated by high-performance liquid chromatography (HPLC). The m/z values of the two products were 856 and 871....

journal_title：Molecular and biochemical parasitology

authors： Hong YL,Yang YZ,Meshnick SR

更新日期：1994-01-01 00:00:00

abstract：:Amastigotes of Leishmania major were isolated from infected mice and radiolabeled for 2 h with [3H]galactose. An acidic [3H]glycoconjugate was extracted from a dilipidated residue fraction with the solvent water/ethanol/diethylether/pyridine/NH4OH (15:15:5:1:0.017). The radioactivity labeled glycoconjugate was found t...

journal_title：Molecular and biochemical parasitology

authors： Turco SJ,Sacks DL

更新日期：1991-03-01 00:00:00

abstract：:The anthelmintic cyclooctadepsipeptide emodepside is effective against nematodes showing resistance against established drug classes. Emodepside exerts its nematicidal effects mainly through its validated target, the tetrameric voltage- and calcium-activated potassium channel SLO-1. Two slo-1 genes were described in T...

journal_title：Molecular and biochemical parasitology

authors： Yilmaz E,Kulke D,von Samson-Himmelstjerna G,Krücken J

更新日期：2015-01-01 00:00:00

abstract：:The infective larval stage of the nematode Trichinella spiralis is an intracellular parasite of skeletal muscle cells. Infection with T. spiralis results in dedifferentiation of the host cell and the formation of a host/parasite complex. A gene encoding a T. spiralis Helix-Loop-helix (HLH) protein with homology to the...

journal_title：Molecular and biochemical parasitology

authors： Connolly B,Trenholme K,Smith DF

更新日期：1996-10-30 00:00:00

abstract：:Topoisomerases are enzymes that participate in many cellular functions involving topological manipulation of DNA strands. There are two types of topoisomerases in the cell: (a) type I topoisomerases; and (b) type II topoisomerases (topo II). Previously we have cloned and sequenced the gene encoding Trypanosoma cruzi t...

journal_title：Molecular and biochemical parasitology

authors： Fragoso SP,Mattei D,Hines JC,Ray D,Goldenberg S

更新日期：1998-08-01 00:00:00

abstract：:The body's defense against schistosome infection can take many forms. For example, upon developing acute schistosomiasis, patients often have fever coinciding with larval maturation, migration and early oviposition. As the infection becomes established, the parasite comes under oxidative stress generated by the host i...

journal_title：Molecular and biochemical parasitology

authors： Aragon AD,Imani RA,Blackburn VR,Cunningham C

更新日期：2008-12-01 00:00:00

abstract：:Schistosoma mansoni schistosomula cultured in the presence of phospholipids showed a decreased sensitivity to the lethal complement-mediated action of anti-schistosome antibodies. Phosphatidyl choline, sphingomyelin and phosphatidyl ethanolamine had a protective action on the schistosomula transformed in vitro by pass...

journal_title：Molecular and biochemical parasitology

authors： Billecocq A

更新日期：1987-09-01 00:00:00

abstract：:Cloned DNA fragments of the ribosomal RNA gene of Schistosoma mansoni hybridise strongly to Echinococcus DNA following restriction endonuclease and Southern transfer analysis. Individuals within a strain of E. granulosus exhibit identical patterns of hybridisation. However, the hybridisation patterns show significant ...

journal_title：Molecular and biochemical parasitology

authors： McManus DP,Simpson AJ

更新日期：1985-11-01 00:00:00

abstract：:The 104 kDa microneme-rhoptry protein (p104) is the only known apical complex organelle-specific protein of Theileria parva. p104 exhibits striking structural similarities to circumsporozoite protein and sporozoite surface protein 2 of Plasmodium yoelii. Their primary sequences contain two hydrophobic segments, locate...

journal_title：Molecular and biochemical parasitology

authors： Ebel T,Gerhards J,Binder BR,Lipp J

更新日期：1999-05-15 00:00:00

abstract：:The degree of polymorphism and the allelic distribution of 2 major Plasmodium falciparum merozoite surface antigens (MSA1 and MSA2) have been analysed in clinical isolates from Colombia. DNA was prepared directly from patients' blood and used in PCR reactions to amplify block 2 of MSA1 and the central region from MSA2...

journal_title：Molecular and biochemical parasitology

authors： Snewin VA,Herrera M,Sanchez G,Scherf A,Langsley G,Herrera S

更新日期：1991-12-01 00:00:00

abstract：:The variant surface glycoproteins (VSGs) of both metacyclic and bloodstream forms of Trypanosoma congolense are shown to be anchored to the plasma membrane through a glycolipid similar to that found in Trypanosoma brucei. Release of soluble VSG from both metacyclic and bloodstream forms is associated with the exposure...

journal_title：Molecular and biochemical parasitology

authors： Ross CA,Cardoso de Almeida ML,Turner MJ

更新日期：1987-01-15 00:00:00

abstract：:Antibodies were affinity purified from crude antiserum by elution from the 24 kDa region of preparative one-dimensional Western blots containing immobilized adult Schistosoma mansoni inner bilayer membrane proteins. They were shown to be specific for a single acidic polypeptide complex, Smgp24, following immunoblottin...

journal_title：Molecular and biochemical parasitology

authors： Karcz SR,Barnard BJ,Podesta RB

更新日期：1988-11-01 00:00:00

abstract：:The major merozoite-piroplasm surface antigen (mMPSA) of Theileria annulata, Tams1, is known to be antigenically diverse. The possession of variable N-linked glycosylation sites and removal of monoclonal antibody 5E1 reactivity by mild periodate treatment suggested, previously, that divergent epitopes may be conferred...

journal_title：Molecular and biochemical parasitology

authors： Katzer F,McKellar S,Ferguson MA,d'Oliveira C,Shiels BR

更新日期：2002-06-01 00:00:00

abstract：:The separation by chromatofocusing of two distinct purine nucleoside cleaving activities from crude extracts of Trypanosoma brucei brucei is described. One catalyzes the reversible phosphorolysis of 5'-deoxy-5'-methylthioadenosine (MeSAdo) and adenosine (Ado) and was designated an MeSAdo/Ado phosphorylase, while the o...

journal_title：Molecular and biochemical parasitology

authors： Ghoda LY,Savarese TM,Northup CH,Parks RE Jr,Garofalo J,Katz L,Ellenbogen BB,Bacchi CJ

更新日期：1988-01-15 00:00:00

abstract：:An immunodominant 72-kDa surface glycoprotein (Gp72) of Trypanosoma cruzi is involved in adhesion of the flagellum to the cell body (Cooper, R, Ribeiro de Jesus, A and Cross, G.A.M (1993) J. Cell Biol. 122, 149-156). We have characterized a gene, flagellum-adhesion glycoprotein genel (fla1), from Trypanosoma brucei th...

journal_title：Molecular and biochemical parasitology

authors： Nozaki T,Haynes PA,Cross GA

更新日期：1996-11-25 00:00:00

abstract：:Schistosoma mansoni is a major causative agent of schistosomiasis, an important parasitic disease that constitutes a severe health problem in developing countries. Even though an effective treatment exists, it does not prevent re-infection and the development of an effective vaccine still remains the most desirable me...

journal_title：Molecular and biochemical parasitology

authors： Rofatto HK,Tararam CA,Borges WC,Wilson RA,Leite LC,Farias LP

更新日期：2009-07-01 00:00:00

abstract：:In the mosquito, Plasmodium sporozoites rupture from oocysts found on the midgut wall, circulate in the hemolymph and invade salivary glands where they wait to be injected into a vertebrate host during a bloodmeal. The mechanisms by which sporozoites specifically attach to and invade salivary glands are not known but ...

journal_title：Molecular and biochemical parasitology

authors： Sidjanski SP,Vanderberg JP,Sinnis P

更新日期：1997-12-01 00:00:00

abstract：:The livers of chickens maintained on a diet containing an effective in vivo level (70 ppm) of arprinocid, an anticoccidial agent, were found to contain 0.64 ppm of this drug and 0.33 ppm of a metabolite, arprinocid-1-N-oxide. When arprinocid was tested against Eimeria tenella in cultures of chick kidney epithelial cel...

journal_title：Molecular and biochemical parasitology

authors： Wang CC,Simashkevich PM

更新日期：1980-10-01 00:00:00

abstract：:Cystic hydatid disease (CHD) is caused by infection with Echinococcus granulosus metacestodes and affects humans and livestock. Proteins secreted or excreted by protoscoleces, pre-adult worms found in the metacestode, are thought to play fundamental roles in the host-parasite relationship. In this work, we performed a...

journal_title：Molecular and biochemical parasitology

authors： Virginio VG,Monteiro KM,Drumond F,de Carvalho MO,Vargas DM,Zaha A,Ferreira HB

更新日期：2012-05-01 00:00:00

abstract：:The complete sequences of the genomic small subunit ribosomal RNA gene from two Phytomonas isolates: one associated with palm pathologies (P. cocos FGuiana) and one found in lactiferous plants with no apparent pathology (P. Euphorbe Senegal), were analyzed. Partial sequences from a number of other Phytomonas isolates ...

journal_title：Molecular and biochemical parasitology

authors： Marché S,Roth C,Philippe H,Dollet M,Baltz T

更新日期：1995-04-01 00:00:00

abstract：:Plasmodium falciparum trophozoites were isolated by mechanical rupture of infected human erythrocytes followed by a series of differential centrifugation steps. After lysis with sonication, the 100 000 x g supernatant of parasites and uninfected host cells was used to determine the specific activities of a number of e...

journal_title：Molecular and biochemical parasitology

authors： Reyes P,Rathod PK,Sanchez DJ,Mrema JE,Rieckmann KH,Heidrich HG

更新日期：1982-05-01 00:00:00

abstract：:The anaerobic, parasitic protist, Trichomonas vaginalis, is characterized by the absence of mitochondria and the presence of double membrane bound organelles called hydrogenosomes. Succinyl-coenzyme A synthetase is a hydrogenosomal enzyme which catalyzes the formation of ATP via substrate-level phosphorylation. We hav...

journal_title：Molecular and biochemical parasitology

authors： Lahti CJ,Bradley PJ,Johnson PJ

更新日期：1994-08-01 00:00:00

abstract：:The primary S(1) subsite specificity of a recombinant cysteine proteinase, CPB2.8 Delta CTE, of Leishmania mexicana was investigated in a systematic way using a series of peptides derived from Abz-KLRFSKQ-EDDnp in which Arg was substituted by all natural amino acids (where Abz is ortho-amino-benzoyl and EDDnp is N-[2,...

journal_title：Molecular and biochemical parasitology

authors： Alves LC,Judice WA,St Hilaire PM,Meldal M,Sanderson SJ,Mottram JC,Coombs GH,Juliano L,Juliano MA

更新日期：2001-08-01 00:00:00

abstract：:The major proteolytic activity of Trypanosoma cruzi is a cathepsin L-like cysteine protease expressed in all stages of the parasite. As an initial step in identifying possible functions of this enzyme in the life cycle of T. cruzi, and examining its potential as a target for rational drug design, two fluoromethyl keto...

journal_title：Molecular and biochemical parasitology

authors： Harth G,Andrews N,Mills AA,Engel JC,Smith R,McKerrow JH

更新日期：1993-03-01 00:00:00

abstract：:Recombinant proteins and synthetic peptides representing various sequences of gp82, a surface glycoprotein of Trypanosoma cruzi metacyclic trypomastigotes implicated in mammalian cell invasion, were used in this study aiming at the identification of the domain(s) of this molecule required for interaction with target c...

journal_title：Molecular and biochemical parasitology

authors： Santori FR,Dorta ML,Juliano L,Juliano MA,da Silveira JF,Ruiz RC,Yoshida N

更新日期：1996-06-01 00:00:00

abstract：:With the rapid spread of drug-resistant strains of Plasmodium falciparum, the development of new antimalarials is an urgent need. As malaria parasites live in a highly pro-oxidant environment, their anti-oxidant defences have frequently been suggested as candidate drug targets. A key point in such defences is the prod...

journal_title：Molecular and biochemical parasitology

authors： Aparicio IM,Marín-Menéndez A,Bell A,Engel PC

更新日期：2010-08-01 00:00:00