Expression of a stage-specific lipophosphoglycan in Leishmania major amastigotes.


:Amastigotes of Leishmania major were isolated from infected mice and radiolabeled for 2 h with [3H]galactose. An acidic [3H]glycoconjugate was extracted from a dilipidated residue fraction with the solvent water/ethanol/diethylether/pyridine/NH4OH (15:15:5:1:0.017). The radioactivity labeled glycoconjugate was found to possess the following characteristics that were similar to the lipophosphoglycan extractable from promastigotes: (i) migrated as a broad band upon electrophoresis on SDS polyacrylamide gels; (ii) deaminated with nitrous acid; and (iii) hydrolyzed with phosphatidylinositol-specific phospholipase C. Furthermore, analysis of the aqueous soluble material released by the latter enzyme revealed a negatively-charged [3H]polysaccharide intermediate in size compared to the analogous portions of LPG isolated from non-infective and metacyclic promastigotes. Most importantly, the [3H]polysaccharide was found to contain phosphate and was susceptible to mild acid hydrolysis, establishing that the intact molecule is a lipophosphoglycan. A structural difference, however, was found in the major, mild acid-generated fragment of the amastigote phosphoglycan, which was larger in size and not as anionic as the analogous fragment from the promastigote phosphoglycans. These results indicate that the amastigotes do express a lipophosphoglycan, but that it is structurally distinct from its promastigote counterparts.


Mol Biochem Parasitol


Turco SJ,Sacks DL




Has Abstract


1991-03-01 00:00:00














  • Three acetylcholinesterases of the pinewood nematode, Bursaphelenchus xylophilus: insights into distinct physiological functions.

    abstract::Acetylcholinesterase (AChE) plays a key role in postsynaptic transmission in most animals. Nematodes encode multiple AChEs, implying its functional diversity. To explore physiological functions of multiple AChEs, three distinct AChEs (BxACE-1, BxACE-2, and BxACE-3) were identified and characterized from the pinewood n...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kang JS,Lee DW,Choi JY,Je YH,Koh YH,Lee SH

    更新日期:2011-02-01 00:00:00

  • Purification and properties of Plasmodium falciparum malate dehydrogenase.

    abstract::Asexual intraerythrocytic Plasmodium falciparum were shown to have a single isoenzyme of malate dehydrogenase. This malate dehydrogenase was purified to apparent homogeneity using a three-step purification protocol. The parasite malate dehydrogenase had an apparent subunit molecular weight of 32 kDa, a pH optimum of 7...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Lang-Unnasch N

    更新日期:1992-01-01 00:00:00

  • Molecular cloning of GRA4, a Toxoplasma gondii dense granule protein, recognized by mucosal IgA antibodies.

    abstract::Clones which were selected from a Toxoplasma gondii expression library with the immune serum from a T. gondii-infected rabbit, were further screened using milk and intestinal secretions from mice which had been orally infected with T. gondii cysts. The gene products of several clones reacted strongly with milk IgA and...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Mevelec MN,Chardès T,Mercereau-Puijalon O,Bourguin I,Achbarou A,Dubremetz JF,Bout D

    更新日期:1992-12-01 00:00:00

  • Mutagenesis study of the glycosylphosphatidylinositol phospholipase C of Trypanosoma brucei.

    abstract::The glycosylphosphatidylinositol phospholipase C (GPI-PLC) from Trypanosoma brucei is particularly effective in hydrolysing the GPI-anchors of some proteins. The enzyme is inhibited by Zn2+ and p-chloromercurylphenylsulphonic acid, both of which can act as sulphydryl reagents, suggesting that a cysteine residue may be...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Carnall N,Webb H,Carrington M

    更新日期:1997-12-15 00:00:00

  • Inhibitory and neutral antibodies to Plasmodium falciparum MSP119 form ring structures with their antigen.

    abstract::Blood-stage malaria vaccine candidates include surface proteins of the merozoite. Antibodies to these proteins may either block essential steps during invasion or render the merozoite susceptible to phagocytosis or complement-mediated degradation. Structural information on merozoite surface proteins complexed to antib...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Dekker C,Uthaipibull C,Calder LJ,Lock M,Grainger M,Morgan WD,Dodson GG,Holder AA

    更新日期:2004-09-01 00:00:00

  • Presence of contaminating mitochondrial DNA from host reticulocytes in experimental infections of Plasmodium berghei.

    abstract::Superposition of two unrelated processes, namely terminal reticulocyte differentiation and synchronous plasmodial development, takes place in experimental infections of Plasmodium berghei. The first process is shown to be responsible for the appearance of some discrete restriction bands of host origin when DNA is extr...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Pace T,Ponzi M,Dore E,Scotti R,Mons B

    更新日期:1989-11-01 00:00:00

  • Regulation of hsp70 expression in Trypanosoma cruzi by temperature and growth phase.

    abstract::The steady-state level of the hsp70 mRNAs of Trypanosoma cruzi cultured at different temperatures and growth conditions has been analyzed by Northern blotting. We show that only one size class of hsp70 mRNA, of about 2.2 kb, is transcribed from the hsp70 cluster and that its transcription is constitutive at 28 degrees...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Requena JM,Jimenez-Ruiz A,Soto M,Assiego R,Santarén JF,Lopez MC,Patarroyo ME,Alonso C

    更新日期:1992-07-01 00:00:00

  • Acute and persistent infection by a transfected Mo7 strain of Babesia bovis.

    abstract::A Mo7-derived Babesia bovis line stably transfected with the gfp-bsd gene was inoculated into two four to five months old calves, while two additional calves were inoculated with Mo7 parasites. Similar mild clinical signs were detected in all calves. B. bovis rap-1 was identified in the bloodstream by PCR four days po...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Suarez CE,Laughery JM,Schneider DA,Sondgeroth KS,McElwain TF

    更新日期:2012-09-01 00:00:00

  • Purification and characterization of three larval excretory-secretory proteins of Dirofilaria immitis.

    abstract::Two proteins were previously described in the excretory-secretory products (ES) collected from Dirofilaria immitis during the molt from the third stage to the fourth stage in vitro. The two proteins were purified using cation exchange and reverse phase HPLC. During the purification of these two proteins, a third prote...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Frank GR,Grieve RB

    更新日期:1996-01-01 00:00:00

  • Gender biased differential alternative splicing patterns of the transcriptional cofactor CA150 gene in Schistosoma mansoni.

    abstract::The complex molecular systems involved in the process of sex-differentiation and fertility in Schistosoma mansoni have not yet been completely described. Using a 4608-element cDNA microarray, we have now determined 90 and 139 genes with significantly (q-value

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: DeMarco R,Oliveira KC,Venancio TM,Verjovski-Almeida S

    更新日期:2006-12-01 00:00:00

  • Accurate transcription of a cloned gene from Babesia bovis in Saccharomyces cerevisiae.

    abstract::Two cloned chromosomal segments of the hemoprotozoan Babesa bovis bearing genes from the polymorphic locus designated BabR were inserted into plasmid vector YCp19, cloned in Escherichia coli and then introduced into Saccharomyces cerevisiae. Both genes were transcribed producing poly(A+) RNAs. In at least one case, th...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kemp DJ,Easton KE,Cowman AF

    更新日期:1984-05-01 00:00:00

  • The Leishmania promastigote surface antigen 2 complex is differentially expressed during the parasite life cycle.

    abstract::The promastigote surface antigen 2 (PSA-2) complex comprises a family of antigenically similar polypeptides of M(r) 96,000, 80,000 and 50,000, anchored to the membrane with glycosylphosphatidylinositol. Although PSA-2 was initially detected only in promastigotes, Northern blot analysis indicated that mRNA transcripts ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Handman E,Osborn AH,Symons F,van Driel R,Cappai R

    更新日期:1995-11-01 00:00:00

  • Substrate specificities of 5'-deoxy-5'-methylthioadenosine phosphorylase from Trypanosoma brucei brucei and mammalian cells.

    abstract::The separation by chromatofocusing of two distinct purine nucleoside cleaving activities from crude extracts of Trypanosoma brucei brucei is described. One catalyzes the reversible phosphorolysis of 5'-deoxy-5'-methylthioadenosine (MeSAdo) and adenosine (Ado) and was designated an MeSAdo/Ado phosphorylase, while the o...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ghoda LY,Savarese TM,Northup CH,Parks RE Jr,Garofalo J,Katz L,Ellenbogen BB,Bacchi CJ

    更新日期:1988-01-15 00:00:00

  • Characterization of a Fasciola gigantica protein carrying two DM9 domains reveals cellular relocalization property.

    abstract::Even at the present age of whole-organism analysis, e.g., genomics, transcriptomics, and proteomics, the biological roles of many proteins remain unresolved. Classified among the proteins of unknown function is a family of proteins harboring repeats of the DM9 domain, a 60-75 amino acids motif first described in a sma...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Phadungsil W,Smooker PM,Vichasri-Grams S,Grams R

    更新日期:2016-01-01 00:00:00

  • Peptide-fluoromethyl ketones arrest intracellular replication and intercellular transmission of Trypanosoma cruzi.

    abstract::The major proteolytic activity of Trypanosoma cruzi is a cathepsin L-like cysteine protease expressed in all stages of the parasite. As an initial step in identifying possible functions of this enzyme in the life cycle of T. cruzi, and examining its potential as a target for rational drug design, two fluoromethyl keto...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Harth G,Andrews N,Mills AA,Engel JC,Smith R,McKerrow JH

    更新日期:1993-03-01 00:00:00

  • Molecular species analysis and quantification of the glycosylphosphatidylinositol intermediate glycolipid C from Trypanosoma brucei.

    abstract::The precursor of the glycosylphosphatidylinositol membrane anchor of the variant surface glycoprotein of Trypanosoma brucei is known as glycolipid A and it has the structure: EtN-PO4-6Man alpha 1-2Man alpha 1-6Man alpha 1-4GlcN alpha 1-6myo-inositol-PO4-(sn-1,2-dimyristoylglycerol). This precursor exists in equilibriu...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Güther ML,Treumann A,Ferguson MA

    更新日期:1996-05-01 00:00:00

  • DNA microarrays for comparative genomics and analysis of gene expression in Trypanosoma cruzi.

    abstract::Trypanosoma cruzi presents high genetic diversity and parasite isolates show remarkable differences in biological parameters. In this study, we evaluated whether DNA microarrays containing CL Brener cDNAs can be used for comparative genomics and for the analysis of gene expression in T. cruzi. We constructed a prototy...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Baptista CS,Vêncio RZ,Abdala S,Valadares MP,Martins C,de Bragança Pereira CA,Zingales B

    更新日期:2004-12-01 00:00:00

  • Extensive polymorphism at the Gp63 locus in field isolates of Leishmania peruviana.

    abstract::Genetic diversity within and between tandemly arrayed copies of the Gp63 gene occurs in laboratory isolates of Leishmania spp., but the extent to which this represents natural genetic diversity has not been assessed. Here, the Gp63 locus is examined in 58 fresh isolates of L. peruviana, and clones derived from them, c...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Espinoza JR,Skinner AC,Davies CR,Llanos-Cuentas A,Arevalo J,Dye C,McMaster WR,Ajioka JW,Blackwell JM

    更新日期:1995-06-01 00:00:00

  • Surface-associated antigens of Brugia malayi L2 and L3 parasites during vector-stage development.

    abstract::Surface and metabolic labeling procedures were used to characterize the composition and the time of expression of Brugia malayi L2 and L3 surface-associated molecules as the larvae develop within the mosquito vector. Larvae were harvested from mosquito tissues at 5 (early L2), 8 (late L2) and 11 (L3) days post-infecti...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ibrahim MS,Richie TL,Scott AL

    更新日期:1992-05-01 00:00:00

  • A surface-labeled 18 kilodalton antigen in Schistosoma mansoni.

    abstract::A mild surface-labeling procedure was applied to various developmental stages of Schistosoma mansoni. An 18 kDa protein was preferentially labeled in freshly transformed schistosomula. The labeled protein was equally present on skin-penetrated and mechanically prepared schistosomula and it disappeared upon digestion o...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Liberti P,Festucci A,Ruppel A,Gigante S,Cioli D

    更新日期:1986-01-01 00:00:00

  • Helminth immunoregulation: the role of parasite secreted proteins in modulating host immunity.

    abstract::Helminths are masterful immunoregulators. A characteristic feature of helminth infection is a Th2-dominated immune response, but stimulation of immunoregulatory cell populations, such as regulatory T cells and alternatively activated macrophages, is equally common. Typically, Th1/17 immunity is blocked and productive ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Hewitson JP,Grainger JR,Maizels RM

    更新日期:2009-09-01 00:00:00

  • The secreted and somatic antigens of the third stage larva of Anisakis simplex, and antigenic relationship with Ascaris suum, Ascaris lumbricoides, and Toxocara canis.

    abstract::The in vitro-released 'excretory/secretory' (ES) and somatic antigens of the third stage (infective) larva of Anisakis simplex were characterised by radioiodination, immunoprecipitation, and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Oral infection with the parasite elicited a substantial IgG antibody...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kennedy MW,Tierney J,Ye P,McMonagle FA,McIntosh A,McLaughlin D,Smith JW

    更新日期:1988-10-01 00:00:00

  • On the DNA content of Trypanosoma cruzi.

    abstract::The DNA contents of three different Trypanosoma cruzi strains were compared by direct microfluorometry. The maximal difference found was 40% of the lowest value. Two of the cloned strains, reported in an earlier study to differ by 48% in their total DNA content, showed a difference of 33%. The kinetoplast of the Y str...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kooy RF,Ashall F,Van der Ploeg M,Overdulve JP

    更新日期:1989-08-01 00:00:00

  • Mechanisms of host seeking by parasitic nematodes.

    abstract::The phylum Nematoda comprises a diverse group of roundworms that includes parasites of vertebrates, invertebrates, and plants. Human-parasitic nematodes infect more than one billion people worldwide and cause some of the most common neglected tropical diseases, particularly in low-resource countries [1]. Parasitic nem...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Gang SS,Hallem EA

    更新日期:2016-07-01 00:00:00

  • Linearized free maxicircle DNA in Crithidia fasciculata is a product of topoisomerase II-mediated cleavage.

    abstract::Linearized free maxicircle DNA, present in detergent lysates of Crithidia fasciculata mitochondria, was thought to be a replication intermediate formed during rolling circle replication of maxicircle DNA. Gel electrophoresis of the linearized free maxicircles indicated that they were slightly larger than the maxicircl...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Carpenter LR,Englund PT

    更新日期:1996-02-01 00:00:00

  • Identification of peripheral membrane proteins associated with the tubo-vesicular network of Plasmodium falciparum infected erythrocytes.

    abstract::During intracellular development of the malarial parasite numerous membranous vesicles appear in the infected erythrocyte cytoplasm between the parasitophorous vacuolar membrane (PVM) and the erythrocyte plasma membrane. In this study we describe the characterization of a monoclonal antibody which recognizes two major...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Martinez SL,Clavijo CA,Winograd E

    更新日期:1998-03-15 00:00:00

  • Characterisation of the gene encoding a 104-kilodalton microneme-rhoptry protein of Theileria parva.

    abstract::A neutralizing antiserum, C16, raised against sporozoites of Theileria parva parva was used to screen a lambda gt11 expression library of T. parva parva (Muguga) genomic DNA fragments. Proteins encoded by one phage clone, lambda TpS-17, were reactive with the C16 antiserum. Detailed characterisation of the DNA insert ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Iams KP,Young JR,Nene V,Desai J,Webster P,ole-MoiYoi OK,Musoke AJ

    更新日期:1990-02-01 00:00:00

  • Heterogeneity of the serine synthetic pathway in Entamoeba species.

    abstract::Phosphoserine phosphatase (PSP) catalyzes the third step of the phosphorylated serine biosynthetic pathway, and occurred multiple times in evolution, while enzymes catalyzing the first and second steps in the pathway have single respective origins. In the present study, we examined the existence of PSP among genus Ent...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Chiba Y,Makiuchi T,Jeelani G,Nozaki T

    更新日期:2016-06-01 00:00:00

  • Proteolytic activity of Plasmodium falciparum subtilisin-like protease 3 on parasite profilin, a multifunctional protein.

    abstract::Subtilisin-like proteases of malaria parasite Plasmodium falciparum (PfSUB1, 2 and 3) are expressed at late asexual blood stages. PfSUB1 and 2 are considered important drug targets due to their essentiality for parasite blood stages and role in merozoite egress and invasion of erythrocytes. We have earlier shown the i...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Alam A,Bhatnagar RK,Relan U,Mukherjee P,Chauhan VS

    更新日期:2013-10-01 00:00:00

  • The role of N-linked carbohydrates in the antigenicity of Taenia solium metacestode glycoproteins of 12, 16 and 18 kD.

    abstract::The glycoproteins of 12-28 kD from Taenia solium metacestodes provide a high specificity and sensitivity for the serological diagnosis of the central nervous system infection, neurocysticercosis. Their widespread use as antigens for routine serological assays will require their production in large and reproducible amo...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Obregón-Henao A,Gil DL,Gómez DI,Sanzón F,Teale JM,Restrepo BI

    更新日期:2001-05-01 00:00:00