Abstract:
:Cryptosporidiosis is an obligate intracellular pathogen causing diarrhea. Merozoite egress is essential for infection to spread between host cells. However, the mechanisms of egress have yet to be defined. We hypothesized that Cyclic GMP-Dependent Protein Kinase G (PKG) may be involved in Cryptosporidium egress. In this study, Cryptosporidium parvum PKG was silenced by using antisense RNA sequences. PKG-silencing significantly inhibited egress of merozoites from infected HCT-8 cells into the supernatant and led to retention of intracellular forms within the host cells. This data identifies PKG as a key mediator of merozoite egress, a key step in the parasite lifecycle.
journal_name
Mol Biochem Parasitoljournal_title
Molecular and biochemical parasitologyauthors
Nava S,Sadiqova A,Castellanos-Gonzalez A,White AC Jrdoi
10.1016/j.molbiopara.2020.111277subject
Has Abstractpub_date
2020-05-01 00:00:00pages
111277eissn
0166-6851issn
1872-9428pii
S0166-6851(20)30041-4journal_volume
237pub_type
杂志文章abstract::Cryptosporidium parvum is a protozoan parasite which produces self-limited disease in immunocompetent hosts and devastating, persistent diarrhea in immunocompromised individuals. There is no effective treatment for cryptosporidiosis and little is known about the basic biology of the organism. Cloning and sequence anal...
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