Abstract：
:Strongyloides stercoralis and S. ratti are intestinal parasitic nematodes infecting rats and humans, respectively. Both present extraordinary life cycles comprising a free-living generation in addition to parasitic stages. In search of molecules possibly involved in parasite-host interaction, we performed mass spectrometry to identify excretory/secretory products of S. ratti. Amongst others we detected homologs of the heat shock proteins HSP10 and HSP60 (Sr-HSP10 and Sr-HSP60). HSPs are well known as chaperones involved in stress responses of cells, but recent studies suggest additional roles of small HSPs for parasite biology including immune modulation. To characterise Sr-HSP10, we cloned its full-length cDNA, analysed the genomic organisation, tested its presumptive role as an interaction partner of Sr-HSP60, studied its transcription in the parasite, and expressed the protein to test its immune responses. The cDNA contains an open reading frame of 330bp encoding a polypeptide of 110 amino acids with an approximate molecular weight of 10kDa. The Sr-HSP10 protein is highly homologous to that of the human pathogen S. stercoralis with only eight amino acid substitutions. Analysis of the genomic organisation of the Sr-HSP10 locus revealed that the gene is linked head-to-head to the gene encoding Sr-HSP60, and both share a bidirectional promoter. RT-PCR experiments indicated potential independent expression of the Sr-HSPs genes. In situ hybridisation results demonstrate Sr-HSP10 transcription in the gut area. Mammalian and yeast two-hybrid assays show dimerisation of Sr-HSP10, but no binding to recombinant Sr-HSP60. Immunisation experiments finally revealed a strong immunogenicity of Sr-HSP10 and provided evidence for a role in regulating the host-parasite interaction.
journal_name
Mol Biochem Parasitoljournal_title
Molecular and biochemical parasitologyauthors
Tazir Y,Steisslinger V,Soblik H,Younis AE,Beckmann S,Grevelding CG,Steen H,Brattig NW,Erttmann KDdoi
10.1016/j.molbiopara.2009.07.007subject
Has Abstractpub_date
2009-12-01 00:00:00pages
149-57issue
2eissn
0166-6851issn
1872-9428pii
S0166-6851(09)00191-1journal_volume
168pub_type
杂志文章abstract：:Previous studies have established that the ISO1 glucose transporter of Leishmania enriettii resides primarily in the flagellar membrane, whereas the ISO2 glucose transporter is located in the pellicular plasma membrane surrounding the cell body. This pronounced difference in subcellular targeting is conferred by the N...
journal_title：Molecular and biochemical parasitology
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更新日期：1989-05-15 00:00:00
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更新日期：1994-02-01 00:00:00
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journal_title：Molecular and biochemical parasitology
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更新日期：2019-03-01 00:00:00
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更新日期：2014-03-01 00:00:00
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journal_title：Molecular and biochemical parasitology
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journal_title：Molecular and biochemical parasitology
pub_type： 杂志文章,评审
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journal_title：Molecular and biochemical parasitology
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journal_title：Molecular and biochemical parasitology
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