Molecular and functional characterisation of the heat shock protein 10 of Strongyloides ratti.


:Strongyloides stercoralis and S. ratti are intestinal parasitic nematodes infecting rats and humans, respectively. Both present extraordinary life cycles comprising a free-living generation in addition to parasitic stages. In search of molecules possibly involved in parasite-host interaction, we performed mass spectrometry to identify excretory/secretory products of S. ratti. Amongst others we detected homologs of the heat shock proteins HSP10 and HSP60 (Sr-HSP10 and Sr-HSP60). HSPs are well known as chaperones involved in stress responses of cells, but recent studies suggest additional roles of small HSPs for parasite biology including immune modulation. To characterise Sr-HSP10, we cloned its full-length cDNA, analysed the genomic organisation, tested its presumptive role as an interaction partner of Sr-HSP60, studied its transcription in the parasite, and expressed the protein to test its immune responses. The cDNA contains an open reading frame of 330bp encoding a polypeptide of 110 amino acids with an approximate molecular weight of 10kDa. The Sr-HSP10 protein is highly homologous to that of the human pathogen S. stercoralis with only eight amino acid substitutions. Analysis of the genomic organisation of the Sr-HSP10 locus revealed that the gene is linked head-to-head to the gene encoding Sr-HSP60, and both share a bidirectional promoter. RT-PCR experiments indicated potential independent expression of the Sr-HSPs genes. In situ hybridisation results demonstrate Sr-HSP10 transcription in the gut area. Mammalian and yeast two-hybrid assays show dimerisation of Sr-HSP10, but no binding to recombinant Sr-HSP60. Immunisation experiments finally revealed a strong immunogenicity of Sr-HSP10 and provided evidence for a role in regulating the host-parasite interaction.


Mol Biochem Parasitol


Tazir Y,Steisslinger V,Soblik H,Younis AE,Beckmann S,Grevelding CG,Steen H,Brattig NW,Erttmann KD




Has Abstract


2009-12-01 00:00:00














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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Köhler P,Bachmann R

    更新日期:1980-04-01 00:00:00

  • Homologous telomeric sequences are present in different species of the genus Plasmodium.

    abstract::The telomeric sequence cloned from Plasmodium berghei (see M. Ponzi et al. (1985) EMBO J. 4, 2991-2995) was tested for species specificity. A telomeric and a subtelomeric fragment of the cloned insert served as separate, labelled probes on pulsed field gradient electrophoretical patterns and on genomic digests from th...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Dore E,Pace T,Ponzi M,Scotti R,Frontali C

    更新日期:1986-11-01 00:00:00

  • Toxoplasma ISP4 is a central IMC sub-compartment protein whose localization depends on palmitoylation but not myristoylation.

    abstract::Apicomplexan parasites utilize a peripheral membrane system called the inner membrane complex (IMC) to facilitate host cell invasion and parasite replication. We recently identified a novel family of Toxoplasma IMC Sub-compartment Proteins (ISP1/2/3) that localize to sub-domains of the IMC using a targeting mechanism ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Fung C,Beck JR,Robertson SD,Gubbels MJ,Bradley PJ

    更新日期:2012-08-01 00:00:00

  • Effects of curcumin on the parasite Schistosoma mansoni: a transcriptomic approach.

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Morais ER,Oliveira KC,Magalhães LG,Moreira EB,Verjovski-Almeida S,Rodrigues V

    更新日期:2013-02-01 00:00:00

  • Leupeptin alters the proteolytic processing of P126, the major parasitophorous vacuole antigen of Plasmodium falciparum.

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Debrabant A,Delplace P

    更新日期:1989-03-01 00:00:00

  • Mitochondrial genomes of Meloidogyne chitwoodi and M. incognita (Nematoda: Tylenchina): comparative analysis, gene order and phylogenetic relationships with other nematodes.

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Humphreys-Pereira DA,Elling AA

    更新日期:2014-03-01 00:00:00

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    abstract::The 31P NMR spectrum of the adult tapeworm, Hymenolepis diminuta, at 37 degrees C during perfusion with physiological saline was composed of 10 peaks. Based on chemical shifts and analysis of worm extracts, the phosphorus components included glucose-6-phosphate, fructose-6-phosphate, phosphorylcholine, glycerophosphor...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Thompson SN,Platzer EG,Lee RW

    更新日期:1987-01-02 00:00:00

  • Characterization and PCR multiplexing of polymorphic microsatellite loci in the whipworm Trichuris arvicolae, parasite of arvicoline rodents and their cross-species utilization in T. muris, parasite of murines.

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Deter J,Galan M,Charbonnel N,Cosson JF

    更新日期:2009-10-01 00:00:00

  • Identification of immunoreactive proteins from the dog heartworm (Dirofilaria immitis) differentially recognized by the sera from dogs with patent or occult infections.

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Oleaga A,Pérez-Sánchez R,Pagés E,Marcos-Atxutegi C,Simón F

    更新日期:2009-08-01 00:00:00

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hines JC,Engel ML,Zhao H,Ray DS

    更新日期:2001-06-01 00:00:00

  • Helminth immunoregulation: the role of parasite secreted proteins in modulating host immunity.

    abstract::Helminths are masterful immunoregulators. A characteristic feature of helminth infection is a Th2-dominated immune response, but stimulation of immunoregulatory cell populations, such as regulatory T cells and alternatively activated macrophages, is equally common. Typically, Th1/17 immunity is blocked and productive ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Hewitson JP,Grainger JR,Maizels RM

    更新日期:2009-09-01 00:00:00

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Stritzke C,Nalaskowski MM,Fanick W,Lin H,Mayr GW

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Robertson CD

    更新日期:1999-09-20 00:00:00

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Navarro M,Cross GA

    更新日期:1998-07-01 00:00:00

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    pub_type: 杂志文章


    authors: Ortner S,Clark CG,Binder M,Scheiner O,Wiedermann G,Duchêne M

    更新日期:1997-05-01 00:00:00

  • Molecular cloning, organellar targeting and developmental expression of mitochondrial chaperone HSP60 in Toxoplasma gondii.

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    pub_type: 杂志文章


    authors: Toursel C,Dzierszinski F,Bernigaud A,Mortuaire M,Tomavo S

    更新日期:2000-12-01 00:00:00

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Epping RJ,Goldstone SD,Ingram LT,Upcroft JA,Ramasamy R,Cooper JA,Bushell GR,Geysen HM

    更新日期:1988-02-01 00:00:00

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Sharma M,Mandal G,Mandal S,Bhattacharjee H,Mukhopadhyay R

    更新日期:2015-06-01 00:00:00

  • A novel dual Dbp5/DDX19 homologue from Plasmodium falciparum requires Q motif for activity.

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Mehta J,Tuteja R

    更新日期:2011-03-01 00:00:00

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Opperdoes FR,Van Roy J

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Jensen BC,Kifer CT,Brekken DL,Randall AC,Wang Q,Drees BL,Parsons M

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kron MA,Wang C,Vodanovic-Jankovic S,Howard OM,Kuhn LA

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ritchie GY,Mungthin M,Green JE,Bray PG,Hawley SR,Ward SA

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Campbell DA,Suyama Y,Simpson L

    更新日期:1989-12-01 00:00:00

  • Leishmania donovani PP2C: Kinetics, structural attributes and in vitro immune response.

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Jakkula P,Qureshi R,Iqbal A,Sagurthi SR,Qureshi IA

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Abrahamsen MS,Johnson RR,Hathaway M,White MW

    更新日期:1995-05-01 00:00:00

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kuang L,Colgrave ML,Bagnall NH,Knox MR,Qian M,Wijffels G

    更新日期:2009-11-01 00:00:00

  • Demonstration of glycosomes (microbodies) in the Bodonid flagellate Trypanoplasma borelli (Protozoa, Kinetoplastida).

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Opperdoes FR,Nohynkova E,Van Schaftingen E,Lambeir AM,Veenhuis M,Van Roy J

    更新日期:1988-08-01 00:00:00

  • Molecular cloning and partial characterization of ribosomal RNA genes from Trypanosoma cruzi.

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hernández R,Díaz-de Léon F,Castañeda M

    更新日期:1988-01-15 00:00:00

  • Molecular cloning and nuclear localization of a histone deacetylase homologue in Plasmodium falciparum.

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Joshi MB,Lin DT,Chiang PH,Goldman ND,Fujioka H,Aikawa M,Syin C

    更新日期:1999-03-15 00:00:00