Schizodeme analysis of Trypanosoma cruzi stocks from South and Central America by analysis of PCR-amplified minicircle variable region sequences.

abstract：:The primary S(1) subsite specificity of a recombinant cysteine proteinase, CPB2.8 Delta CTE, of Leishmania mexicana was investigated in a systematic way using a series of peptides derived from Abz-KLRFSKQ-EDDnp in which Arg was substituted by all natural amino acids (where Abz is ortho-amino-benzoyl and EDDnp is N-[2,...

journal_title：Molecular and biochemical parasitology

authors： Alves LC,Judice WA,St Hilaire PM,Meldal M,Sanderson SJ,Mottram JC,Coombs GH,Juliano L,Juliano MA

更新日期：2001-08-01 00:00:00

abstract：:The tick-borne protozoan parasite, Babesia bovis is one of the causes of bovine babesiosis, an economically important disease of cattle in tropical and sub-tropical countries. Using the recently published genome sequence of the parasite, we developed a panel of eight mini- and micro-satellite markers and used these to...

journal_title：Molecular and biochemical parasitology

authors： Simuunza M,Bilgic H,Karagenc T,Syakalima M,Shiels B,Tait A,Weir W

更新日期：2011-06-01 00:00:00

abstract：:Distinct 3'- and 5'-nucleotidase activities were localized to the surface membrane of Leishmania donovani promastigotes using enzymatic reactions with live intact cells and fine structure enzyme-mediated cytochemical reactions with intact cells, cell homogenates, and isolated surface membranes. The results indicated t...

journal_title：Molecular and biochemical parasitology

authors： Dwyer DM,Gottlieb M

更新日期：1984-02-01 00:00:00

abstract：:The expressed sequence tag (EST) dataset of Toxoplasma gondii provides a wealth of information towards gene discovery. The complete cDNA and genomic sequence of EST tgc050 locus shows that it contains five copies of the conserved thrombospondin (TSP)-like motif present in a number of molecules with adhesive properties...

journal_title：Molecular and biochemical parasitology

authors： Wan KL,Carruthers VB,Sibley LD,Ajioka JW

更新日期：1997-02-01 00:00:00

abstract：:We have isolated and characterized a gene encoding a novel GTP-binding protein of the GTPase superfamily in the filarial parasites Brugia malayi and Onchocerca volvulus. The deduced amino acid sequence of the cloned molecule has approximately 30% overall homology to ras proteins and approximately 90% homology to the '...

journal_title：Molecular and biochemical parasitology

authors： Dissanayake S,Xu M,Piessens WF

更新日期：1992-12-01 00:00:00

abstract：:Procyclic Trypanosoma brucei brucei strain 366D is susceptible to DL-alpha-difluoromethylornithine (DFMO) with an in vitro ED50 value of 225 microM. A mutant of the procyclic strain resistant to 20 mM of DFMO was isolated by serial in vitro passages of the organisms in increasing concentrations of the drug. Drug resis...

journal_title：Molecular and biochemical parasitology

authors： Phillips MA,Wang CC

更新日期：1987-01-02 00:00:00

abstract：:A Trypanosoma cruzi genomic expression library was screened with a pool of sera obtained from chronic chagasic patients. The recombinant antigen (Tc40) isolated from this library reacted with a large number of serum samples of chronic chagasic patients, suggesting that the presence of anti-Tc40 antibodies may be speci...

journal_title：Molecular and biochemical parasitology

authors： Lesénéchal M,Duret L,Cano MI,Mortara RA,Jolivet M,Camargo ME,da Silveira JF,Paranhos-Baccalà G

更新日期：1997-08-01 00:00:00

abstract：:In order to clarify the interpretation of molecular karyotype polymorphisms in Leishmania, the three smallest chromosomes from five cloned strains of Leishmania infantum were identified by chromosome-specific anonymous DNA probes. The presence in the same clone of homologous chromosomes of a different size was demonst...

journal_title：Molecular and biochemical parasitology

authors： Blaineau C,Bastien P,Rioux JA,Roizès G,Pagès M

更新日期：1991-06-01 00:00:00

abstract：:The scarcity of molecular tools for genetic manipulation is a critical obstacle for functional genomics studies on Trypanosoma cruzi. The current study adapted an inducible site-specific recombination system based on Dimerizable CRE recombinase (DiCRE). Two vectors for stable transfection were created, a first one to ...

journal_title：Molecular and biochemical parasitology

authors： Kangussu-Marcolino MM,Cunha AP,Avila AR,Herman JP,DaRocha WD

更新日期：2014-12-01 00:00:00

abstract：:A family of Schistosoma mansoni proteins (18-22 kDa, pI 5.3-5.8) are biosynthesized in juvenile worms and immunoprecipitated by antibodies uniquely present in protective Fischer rat antiserum. A cDNA clone, lambda gt11-40, expressing epitopes common to this protein family was used to obtain a genomic DNA clone, by hyb...

journal_title：Molecular and biochemical parasitology

authors： el-Sherbeini M,Ramadan N,Bostian KA,Knopf PM

更新日期：1991-11-01 00:00:00

abstract：:The karyotype and chromosomes of the human malaria parasite Plasmodium falciparum have been well characterized in recent years. Here we present karyotype maps of the three other human malaria species, P. vivax, P. malariae and P. ovale. Chromosomes of these species were found to be of significantly higher molecular we...

journal_title：Molecular and biochemical parasitology

authors： Carlton JM,Galinski MR,Barnwell JW,Dame JB

更新日期：1999-06-25 00:00:00

abstract：:The activities of carbamoylphosphate synthase, aspartate transcarbamoylase, dihydroorotase, orotate phosphoribosyl transferase and orotidine-5'-phosphate decarboxylase, five of the six enzymes of pyrimidine biosynthesis, have been measured in Crithidia fasciculata, Trypanosoma cruzi, Leishmania major, Trichomonas vagi...

journal_title：Molecular and biochemical parasitology

authors： Hill B,Kilsby J,Rogerson GW,McIntosh RT,Ginger CD

更新日期：1981-02-01 00:00:00

abstract：:Genomic structure has been determined for a gene encoding a host-protective antigen of the parasitic platyhelminth Taenia ovis. An incomplete cDNA, known as 45W, encodes the protective antigen. Southern hybridisation experiments using 45W cDNA as a probe, revealed that the 45W gene was a member of a multigene family. ...

journal_title：Molecular and biochemical parasitology

authors： Waterkeyn JG,Lightowlers MW,Coppel R,Cowman AF

更新日期：1995-07-01 00:00:00

abstract：:The tegumental membrane of Taenia crassiceps cysticerci contains an ATP-diphosphohydrolase (EC 3.6.1.5) which hydrolyzes purine and pyrimidine nucleoside 5'-di- and 5'-triphosphates at an optimum pH of 8.5. It is Mg(2+)-dependent and insensitive to classical ATPase and phosphatase inhibitors. In solubilized tegumental...

journal_title：Molecular and biochemical parasitology

authors： Guevara-Flores A,Olvera-Sánchez S,Gómez-Concha C,Juárez O,Esparza-Perusquía M,Pardo JP,Mendoza-Hernández G,Martínez F,Flores-Herrera O

更新日期：2008-12-01 00:00:00

abstract：:Plasmodium falciparum extensively modifies the infected red blood cell (RBC), resulting in changes in deformability, shape and surface properties. These alterations suggest that the RBC cytoskeleton is a major target for modification during infection. However, the molecular mechanisms leading to these changes are larg...

journal_title：Molecular and biochemical parasitology

authors： Shakya B,Penn WD,Nakayasu ES,LaCount DJ

更新日期：2017-09-01 00:00:00

abstract：:The Plasmodium MSP-1 is a promising malaria vaccine candidate. However, the highly polymorphic nature of the MSP-1 gene (msp1) presents a potential obstacle for effective vaccine development. To investigate the evolutionary history of msp1 polymorphism in P. vivax, we construct phylogenetic trees of msp1 from P. vivax...

journal_title：Molecular and biochemical parasitology

authors： Tanabe K,Escalante A,Sakihama N,Honda M,Arisue N,Horii T,Culleton R,Hayakawa T,Hashimoto T,Longacre S,Pathirana S,Handunnetti S,Kishino H

更新日期：2007-11-01 00:00:00

abstract：:The aspartic protease cathepsin D (Clan AA, Family A1) is expressed in the schistosome gut where it plays an apical role in the digestion of hemoglobin released from ingested erythrocytes. In this report, RNA interference approaches were employed to investigate the effects of knockdown of schistosome cathepsin D. Cult...

journal_title：Molecular and biochemical parasitology

authors： Morales ME,Rinaldi G,Gobert GN,Kines KJ,Tort JF,Brindley PJ

更新日期：2008-02-01 00:00:00

abstract：:Some restriction fragments of kinetoplast minicircles from several trypanosomatid species (Leishmania tarentolae, Trypanosoma brucei, T. equiperdum, Herpetomonas muscarum, Crithidia fasciculata, but not T. cruzi) migrate anomalously on polyacrylamide gels. This behavior is probably due to a natural curvature of the he...

journal_title：Molecular and biochemical parasitology

authors： Ntambi JM,Marini JC,Bangs JD,Hajduk SL,Jimenez HE,Kitchin PA,Klein VA,Ryan KA,Englund PT

更新日期：1984-07-01 00:00:00

abstract：:We have assessed the phylogenetic status of the Trypanosoma cruzi Genome Project CL Brener reference strain by multilocus enzyme electrophoresis (MLEE) and multiprimer random amplified polymorphic DNA (RAPD) including a set of cloned stocks representative of the whole genetic diversity of T. cruzi. MLEE and RAPD data ...

journal_title：Molecular and biochemical parasitology

authors： Brisse S,Barnabé C,Bañuls AL,Sidibé I,Noël S,Tibayrenc M

更新日期：1998-05-01 00:00:00

abstract：:Messenger RNA has been isolated from adults and eggs of Schistosoma mansoni and translated in vitro in mRNA dependent rabbit reticulocyte lysate. The in vitro translation products have been immunoprecipitated using a wide variety of hyperimmune and infection sera from rabbits, mice and humans. A large number of in vit...

journal_title：Molecular and biochemical parasitology

authors： Taylor DW,Cordingley JS,Butterworth AE

更新日期：1984-03-01 00:00:00

abstract：:We have previously provided evidence for a pathway in Plasmodium falciparum-infected erythrocytes, coined the parasitophorous duct pathway, which provides serum (macro)molecules direct access to intraerythrocytic parasites . The present study addresses the purity of the fluorescent macromolecules used to define the du...

journal_title：Molecular and biochemical parasitology

authors： Goodyer ID,Pouvelle B,Schneider TG,Trelka DP,Taraschi TF

更新日期：1997-07-01 00:00:00

abstract：:As genomic sequencing of Leishmania nears completion, functional analyses that provide a global genetic perspective on biological processes are important. Despite polycistronic transcription, RNA transcript abundance can be measured using microarrays. To provide a resource to evaluate cDNA arrays, we undertook 5' expr...

journal_title：Molecular and biochemical parasitology

authors： Almeida R,Gilmartin BJ,McCann SH,Norrish A,Ivens AC,Lawson D,Levick MP,Smith DF,Dyall SD,Vetrie D,Freeman TC,Coulson RM,Sampaio I,Schneider H,Blackwell JM

更新日期：2004-07-01 00:00:00

abstract：:Malaria transmission occurs during a blood-meal of an infected Anopheles mosquito. Visualization and quantification of sporozoites along the journey from the mosquito midgut, where they develop, to the vertebrate liver, their final target organ, is important for understanding many aspects of sporozoite biology. Here w...

journal_title：Molecular and biochemical parasitology

authors： Engelmann S,Sinnis P,Matuschewski K

更新日期：2006-03-01 00:00:00

abstract：:Leishmania major promastigotes grown in late log phase were incubated with glucose as sole exogenous carbon source in the presence of 5% CO2 and the amounts of glucose consumed and of the major products formed--succinate, pyruvate, alanine, acetate, glycerol, and D-lactate--were measured as a function of pO2. Glucose ...

journal_title：Molecular and biochemical parasitology

authors： Keegan F,Blum JJ

更新日期：1990-03-01 00:00:00

abstract：:Cryptosporidiosis is an obligate intracellular pathogen causing diarrhea. Merozoite egress is essential for infection to spread between host cells. However, the mechanisms of egress have yet to be defined. We hypothesized that Cyclic GMP-Dependent Protein Kinase G (PKG) may be involved in Cryptosporidium egress. In th...

journal_title：Molecular and biochemical parasitology

authors： Nava S,Sadiqova A,Castellanos-Gonzalez A,White AC Jr

更新日期：2020-05-01 00:00:00

abstract：:The gender of cercarial larvae can only be determined using molecular methods. End point PCR methods that amplify repetitive markers on the W chromosome of the female (ZW) parasites have been developed, but sometimes results are ambiguous or incorrect. To more effectively distinguish sexes, and to determine why end po...

journal_title：Molecular and biochemical parasitology

authors： Chevalier FD,Le Clec'h W,Alves de Mattos AC,LoVerde PT,Anderson TJ

更新日期：2016-01-01 00:00:00

abstract：:Plasmodium falciparum merozoites invade erythrocytes using a range of alternative ligands that includes erythrocyte binding antigenic proteins (EBAs) and reticulocyte binding protein homologues (Rh). Variation in the expression of some of these genes among culture-adapted parasite lines correlates with the use of diff...

journal_title：Molecular and biochemical parasitology

authors： Nery S,Deans AM,Mosobo M,Marsh K,Rowe JA,Conway DJ

更新日期：2006-10-01 00:00:00

abstract：:Seven beta-naphthylamine-linked peptides were tested as substrates for a previously described thiol proteinase of adult Schistosoma mansoni. The enzyme was not active on carbobenoxy-arginyl-arginyl-4-methoxy-2-naphthylamide and carbobenzoxy-alanyl-arginyl-arginyl-4-methoxy-2-naphthylamide. Enzyme activity was maximal ...

journal_title：Molecular and biochemical parasitology

authors： Dresden MH,Rutledge ML,Chappell CL

更新日期：1981-11-01 00:00:00

abstract：:The DNA contents of three different Trypanosoma cruzi strains were compared by direct microfluorometry. The maximal difference found was 40% of the lowest value. Two of the cloned strains, reported in an earlier study to differ by 48% in their total DNA content, showed a difference of 33%. The kinetoplast of the Y str...

journal_title：Molecular and biochemical parasitology

authors： Kooy RF,Ashall F,Van der Ploeg M,Overdulve JP

更新日期：1989-08-01 00:00:00

abstract：:After a single intravenous injection of suramin the rate of removal of the drug from the plasma into other tissue compartments of the rat is independent of initial concentration. The data can be fitted to the sum of two exponential functions, consistent with a two-compartment, open model system. Trypanosomes take up o...

journal_title：Molecular and biochemical parasitology

authors： Fairlamb AH,Bowman IB

更新日期：1980-10-01 00:00:00