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Real-time PCR for sexing Schistosoma mansoni cercariae.

Abstract：

:The gender of cercarial larvae can only be determined using molecular methods. End point PCR methods that amplify repetitive markers on the W chromosome of the female (ZW) parasites have been developed, but sometimes results are ambiguous or incorrect. To more effectively distinguish sexes, and to determine why end point PCR can be incorrect, we quantified the W6 repeat sequence and a specific Z chromosome gene using real-time PCR. The ratio between copy number of W6 and a Z chromosome marker unambiguously identifies gender: females have higher ratios (421-4371) than males (0-21). However, some males have low numbers of W6 elements in their genome, and qPCR demonstrated significantly higher W6/Z marker ratios for male genotypes giving ambiguous end point PCR results compared with males giving clear end point results. The quantitative PCR sexing method developed will be particularly useful where reliable sexing of cercariae is critical, for example when staging genetic crosses.

journal_name

Mol Biochem Parasitol

journal_title

Molecular and biochemical parasitology

authors

Chevalier FD,Le Clec'h W,Alves de Mattos AC,LoVerde PT,Anderson TJ

doi

10.1016/j.molbiopara.2016.03.010

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

35-8

issue

1-2

eissn

0166-6851

issn

1872-9428

pii

S0166-6851(16)30027-5

journal_volume

205

pub_type

杂志文章

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