The effects of p-chloromercuriphenylsulfonic acid on Trypanosoma cruzi infection of mammalian host cells in vitro.


:Treatment of Trypanosoma cruzi blood trypomastigotes with p-chloromercuriphenylsulfonic acid (PCMS) increased the association of the parasite with either mouse resident peritoneal macrophages or rat heart myoblasts in vitro. The effect was evidenced by elevation of both the percentage of host cells with parasites and the number of flagellates per 100 cells. The effect of PCMS appeared to be largely on the process of parasite penetration rather than surface binding as it was seen at 37 degrees C but not at 4 degrees C. A short pretreatment time, 5 min, was sufficient to elicit the enhancement, suggesting that the primary effect of PCMS might be at the parasite's cell surface. The PCMS effect was reversible as the parasite returned to normal levels of association with the host cells in less than 4 h after removal of excess PCMS. That sulfhydryl groups were involved in the PCMS effect was indicated by the abilities of excess cysteine and glutathione to block it. These results suggest a role for free sulfhydryl groups on the parasite surface in the process of host cell invasion.


Mol Biochem Parasitol


Ayala A,Kierszenbaum F




Has Abstract


1987-02-01 00:00:00














  • Heat shock and stress response in Giardia lamblia.

    abstract::The heat shock response was studied as a model for control of gene expression and protein synthesis in Giardia lamblia. Cultured trophozoites were metabolically labelled with [35S]methionine, and proteins were analysed by SDS-polyacrylamide gel electrophoresis and fluorography. A temperature shift from 37 degrees C to...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Lindley TA,Chakraborty PR,Edlind TD

    更新日期:1988-03-01 00:00:00

  • TbARF1 influences lysosomal function but not endocytosis in procyclic stage Trypanosoma brucei.

    abstract::The ADP ribosylation factors (Arfs) are a highly conserved subfamily of the Ras small GTPases with crucial roles in vesicle budding and membrane trafficking. Unlike in other eukaryotes, the orthologue of Arf1 in the host bloodstream form of Trypanosoma brucei is essential for the maintenance of endocytosis. In contras...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Price HP,Stark M,Smith B,Smith DF

    更新日期:2007-10-01 00:00:00

  • RNA interference of Schistosoma mansoni cathepsin D, the apical enzyme of the hemoglobin proteolysis cascade.

    abstract::The aspartic protease cathepsin D (Clan AA, Family A1) is expressed in the schistosome gut where it plays an apical role in the digestion of hemoglobin released from ingested erythrocytes. In this report, RNA interference approaches were employed to investigate the effects of knockdown of schistosome cathepsin D. Cult...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Morales ME,Rinaldi G,Gobert GN,Kines KJ,Tort JF,Brindley PJ

    更新日期:2008-02-01 00:00:00

  • Some kinetic properties of pyruvate kinase from Trypanosoma brucei: influence of pH and fructose-1,6-diphosphate.

    abstract::The influence of pH on the activity of purified pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC from Trypanosoma brucei has been studied. The Km for the coenzyme ADP is pH-dependent and shows the involvement of a dissociable group on the free enzyme with a pKa of 6.5-6.7. The cooperative interaction...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Flynn IW,Bowman IB

    更新日期:1981-11-01 00:00:00

  • Expression profiling of the Leishmania life cycle: cDNA arrays identify developmentally regulated genes present but not annotated in the genome.

    abstract::As genomic sequencing of Leishmania nears completion, functional analyses that provide a global genetic perspective on biological processes are important. Despite polycistronic transcription, RNA transcript abundance can be measured using microarrays. To provide a resource to evaluate cDNA arrays, we undertook 5' expr...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Almeida R,Gilmartin BJ,McCann SH,Norrish A,Ivens AC,Lawson D,Levick MP,Smith DF,Dyall SD,Vetrie D,Freeman TC,Coulson RM,Sampaio I,Schneider H,Blackwell JM

    更新日期:2004-07-01 00:00:00

  • Trypanosoma cruzi poly-zinc finger protein: a novel DNA/RNA-binding CCHC-zinc finger protein.

    abstract::A poly-zinc finger protein, designated PZFP1 was identified in Trypanosoma cruzi for the first time. The protein has 191 amino acids, contains seven motifs Cys(X)(2)Cys(X)(4)His(X)(4)Cys. A recombinant PZFP1 was generated in E. coli and the expected 21kDa polypeptide co-purified with two other inducible products of ab...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Espinosa JM,Portal D,Lobo GS,Pereira CA,Alonso GD,Gómez EB,Lan GH,Pomar RV,Flawiá MM,Torres HN

    更新日期:2003-09-01 00:00:00

  • Identification of antigens of Onchocerca volvulus and Onchocerca gibsoni for diagnostic use.

    abstract::Adults of Onchocerca volvulus and Onchocerca gibsoni were identically fractionated into a surface-enriched fraction, a phosphate buffered saline (PBS) extract and a PBS insoluble-detergent soluble fraction. Glycoproteins were prepared from these extracts and all fractions were examined by the Western blot technique us...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Cabrera Z,Parkhouse RM

    更新日期:1986-09-01 00:00:00

  • RNA primer removal and gap filling on a model minicircle replication intermediate.

    abstract::Replication of kinetoplast DNA minicircles in Crithidia fasciculata occurs by a unidirectional mechanism involving continuous synthesis of one strand (L strand) and discontinuous synthesis of the complementary strand (H strand). L-strands are initiated by RNA priming at alternate origins (A and B) resulting in daughte...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hines JC,Engel ML,Zhao H,Ray DS

    更新日期:2001-06-01 00:00:00

  • Expression and functional characterization of a giant Type I fatty acid synthase (CpFAS1) gene from Cryptosporidium parvum.

    abstract::A 25-kb CpFAS1 gene from Cryptosporidium parvum has been engineered and expressed as five individual maltose-binding protein (MBP)-fusion proteins: an N-terminal loading unit, three fatty acyl elongation modules, and a C-terminal reductase. Enzymatic activities of all domains (except the reductase) were individually a...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Zhu G,Li Y,Cai X,Millership JJ,Marchewka MJ,Keithly JS

    更新日期:2004-03-01 00:00:00

  • Gene disruption reveals a dispensable role for plasmepsin VII in the Plasmodium berghei life cycle.

    abstract::Plasmepsins (PM), aspartic proteases of Plasmodium, comprises a family of ten proteins that perform critical functions in Plasmodium life cycle. Except VII and VIII, functions of the remaining plasmepsin members have been well characterized. Here, we have generated a mutant parasite lacking PM VII in Plasmodium berghe...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Mastan BS,Kumari A,Gupta D,Mishra S,Kumar KA

    更新日期:2014-06-01 00:00:00

  • Loss of matrix metalloproteinase 9 activity in Theileria annulata-attenuated cells is at the transcriptional level and is associated with differentially expressed AP-1 species.

    abstract::The schizont stage of the protozoan parasite Theileria annulata reversibly transforms bovine monocytes into an immortalised and metastatic state. We have been studying T. annulata induction of host matrix metalloproteinases (MMP) which are involved in parasite dissemination and pathogenesis. We have observed that prol...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Adamson R,Logan M,Kinnaird J,Langsley G,Hall R

    更新日期:2000-02-25 00:00:00

  • Molecular species analysis and quantification of the glycosylphosphatidylinositol intermediate glycolipid C from Trypanosoma brucei.

    abstract::The precursor of the glycosylphosphatidylinositol membrane anchor of the variant surface glycoprotein of Trypanosoma brucei is known as glycolipid A and it has the structure: EtN-PO4-6Man alpha 1-2Man alpha 1-6Man alpha 1-4GlcN alpha 1-6myo-inositol-PO4-(sn-1,2-dimyristoylglycerol). This precursor exists in equilibriu...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Güther ML,Treumann A,Ferguson MA

    更新日期:1996-05-01 00:00:00

  • Biochemical and genetic analysis of the distinct proliferating cell nuclear antigens of Toxoplasma gondii.

    abstract::The apicomplexa parasite Toxoplasma gondii expresses two distinct proliferating cell nuclear antigens (PCNA) that exhibit distinct patterns of subcellular localization during tachyzoite growth. In all cell cycle phases, TgPCNA1 is concentrated in the nucleus, while TgPCNA2 is only concentrated in the nucleus during S-...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Guerini MN,Behnke MS,White MW

    更新日期:2005-07-01 00:00:00

  • Surface epitope localization and gene structure of a Babesia bovis 44-kilodalton variable merozoite surface antigen.

    abstract::Variation of Babesia bovis merozoite surface antigens occurs among geographic strains of the parasite. In this and a concurrent report, we investigate this variation at the gene and protein level. Using a monoclonal antibody (mAb 23/70.174), B. bovis gene sequences were identified that encoded a surface epitope of a 4...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Jasmer DP,Reduker DW,Hines SA,Perryman LE,McGuire TC

    更新日期:1992-10-01 00:00:00

  • Acute and persistent infection by a transfected Mo7 strain of Babesia bovis.

    abstract::A Mo7-derived Babesia bovis line stably transfected with the gfp-bsd gene was inoculated into two four to five months old calves, while two additional calves were inoculated with Mo7 parasites. Similar mild clinical signs were detected in all calves. B. bovis rap-1 was identified in the bloodstream by PCR four days po...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Suarez CE,Laughery JM,Schneider DA,Sondgeroth KS,McElwain TF

    更新日期:2012-09-01 00:00:00

  • Molecular epidemiology of giardiasis.

    abstract::Giardia duodenalis is a widespread parasite of mammalian species, including humans. Due to its invariant morphology, investigation on aspects such as host specificity and transmission patterns requires a direct genetic characterization of cysts/trophozoites from host samples. A number of molecular assays have been dev...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Cacciò SM,Ryan U

    更新日期:2008-08-01 00:00:00

  • Episomal and integrated maintenance of foreign DNA in Giardia lamblia.

    abstract::Giardia lamblia is an early diverging eukaryote which causes gastrointestinal disease throughout the world. Different subgroups of Giardia have been defined based on several biochemical and genetic criteria. We have developed a method for stably introducing DNA into the nuclei of the parasite using puromycin acetyltra...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Singer SM,Yee J,Nash TE

    更新日期:1998-04-01 00:00:00

  • Perturbation of sterol biosynthesis by itraconazole and ketoconazole in Leishmania mexicana mexicana infected macrophages.

    abstract::The azole antifungals ketoconazole and itraconazole possess in vitro antileishmanial activity against Leishmania mexicana mexicana amastigotes in macrophages (cell line J774G8). As in yeast and fungi, the activity is likely to be due to inhibition of the cytochrome P-450-dependent 14 alpha-demethylation of lanosterol ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hart DT,Lauwers WJ,Willemsens G,Vanden Bossche H,Opperdoes FR

    更新日期:1989-03-01 00:00:00

  • The cyst wall of Entamoeba invadens contains chitosan (deacetylated chitin).

    abstract::The cyst wall of Entamoeba invadens (Ei), a model for the human pathogen Entamoeba histolytica, contains chitin, which is a homopolymer of beta-1, 4-linked N-acetyl-glucosamine (GlcNAc). In fungi and in bacteria that make nodulation factors, chitin deacetylases make chitosan, which is a mixture of GlcNAc and glucosami...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Das S,Van Dellen K,Bulik D,Magnelli P,Cui J,Head J,Robbins PW,Samuelson J

    更新日期:2006-07-01 00:00:00

  • A high level of mixed Trypanosoma brucei infections in tsetse flies detected by three hypervariable minisatellites.

    abstract::The issue of whether genetic exchange occurs at a significant frequency in natural populations of Trypanosoma brucei is controversial and one of the arguments against a high frequency has been the apparent lack of host infections with mixtures of trypanosome genotypes. Three minisatellite markers (MS42, CRAM, 292) wit...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: MacLeod A,Turner CM,Tait A

    更新日期:1999-08-20 00:00:00

  • Molecular basis of binding of the Plasmodium falciparum receptor BAEBL to erythrocyte receptor glycophorin C.

    abstract::Plasmodium falciparum invades human erythrocytes by redundant pathways. Unlike Plasmodium vivax that has one Duffy Binding-Like (DBL) receptor, P. falciparum has four members of the DBL receptor family. Furthermore, one of these DBL genes, BAEBL, has polymorphisms at four amino acids in region II; each polymorphism bi...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Jiang L,Duriseti S,Sun P,Miller LH

    更新日期:2009-11-01 00:00:00

  • Transcribing and replicating particles in a double-stranded RNA virus from Leishmania.

    abstract::During the replicative cycle of many double-stranded RNA viruses, transcription of particles with a double-stranded RNA genome alternates with replication of particles containing a single-stranded genome. In virions infecting some strains of Leishmania guyanensis the putative transcriptase and replicase activities of ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Weeks RS,Patterson JL,Stuart K,Widmer G

    更新日期:1992-06-01 00:00:00

  • Molecular cloning and partial characterization of ribosomal RNA genes from Trypanosoma cruzi.

    abstract::To further analyze the organization of the nuclear rDNA locus in Trypanosoma cruzi, genomic recombinant plasmid clones were constructed and isolated after hybridization with rRNA molecules as hybridization probes. Approximately 11 kilobase pairs from the cistron were cloned in three recombinant plasmids carrying adjac...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hernández R,Díaz-de Léon F,Castañeda M

    更新日期:1988-01-15 00:00:00

  • Characterisation of the novel junctions of two minichromosomes of Leishmania major.

    abstract::The minichromosomes M210 and M230 of Leishmania major are linear amplicons which have an inverted repeat structure. We report here the molecular cloning of the megabasic chromosome region where M210 and M230 recombination points are located. The analysis of this chromosomal region revealed a 374 bp fragment directly r...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ortiz G,Segovia M

    更新日期:1996-11-25 00:00:00

  • Membrane modifications in erythrocytes parasitized by Plasmodium falciparum.

    abstract::Plasmodium falciparum malaria parasites invade human red blood cells and immediately begin making significant alterations to the structure of the erythrocyte. These alterations facilitate the movement of nutrients into, and waste products and parasite-derived proteins out of the cell to meet the needs of the growing p...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Deitsch KW,Wellems TE

    更新日期:1996-02-01 00:00:00

  • Purification and characterization of the Giardia lamblia double-stranded RNA virus.

    abstract::The dsRNA virus which infects some strains of Giardia lamblia has been purified and characterized with respect to its effect on growth of the parasite. Extensive purification of the virus from G. lamblia growth medium was accomplished by Millipore filtration and two successive CsCl gradient centrifugations. The purifi...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Miller RL,Wang AL,Wang CC

    更新日期:1988-04-01 00:00:00

  • Occurrence and transfection of a Giardia virus.

    abstract::The presence of virus-derived RNA was investigated in 38 axenically growing Giardia isolates from different geographic areas. The RNA virus was demonstrated in Giardia strains from humans in the U.S.A., England and the majority of strains from Poland. Two strains isolated respectively from a cat and a cavia also conta...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: De Jonckheere JF,Gordts B

    更新日期:1987-02-01 00:00:00

  • Filarial parasites contain a ras homolog of the TC4/ran/Spil family.

    abstract::We have isolated and characterized a gene encoding a novel GTP-binding protein of the GTPase superfamily in the filarial parasites Brugia malayi and Onchocerca volvulus. The deduced amino acid sequence of the cloned molecule has approximately 30% overall homology to ras proteins and approximately 90% homology to the '...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Dissanayake S,Xu M,Piessens WF

    更新日期:1992-12-01 00:00:00

  • Biochemical analysis, gene structure and localization of the 24 kDa glutathione S-transferase from Onchocerca volvulus.

    abstract::Survival of Onchocerca volvulus, a pathogenic human filarial parasite, is likely to depend upon the detoxification activities of the glutathione S-transferases (GSTs). The 24 kDa O. volvulus GST, OvGST2, was expressed in a bacterial system and the recombinant protein was purified to homogeneity by affinity chromatogra...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Liebau E,Wildenburg G,Brophy PM,Walter RD,Henkle-Dührsen K

    更新日期:1996-09-01 00:00:00

  • The subcellular localisation of trypanosome RRP6 and its association with the exosome.

    abstract::The exosome, a complex of 3'-exoribonucleases and associated proteins, is involved in the degradation of eukaryotic mRNAs in the cytoplasm, and has RNA processing and quality control functions in the nucleus. In yeast, the nuclear exosome differs from the cytoplasmic one in that it contains an additional non-essential...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Haile S,Cristodero M,Clayton C,Estévez AM

    更新日期:2007-01-01 00:00:00