Cytochrome c and c1 heme lyases are essential in Plasmodium berghei.

abstract：:The exosome, a complex of 3'-exoribonucleases and associated proteins, is involved in the degradation of eukaryotic mRNAs in the cytoplasm, and has RNA processing and quality control functions in the nucleus. In yeast, the nuclear exosome differs from the cytoplasmic one in that it contains an additional non-essential...

journal_title：Molecular and biochemical parasitology

authors： Haile S,Cristodero M,Clayton C,Estévez AM

更新日期：2007-01-01 00:00:00

abstract：:A mild surface-labeling procedure was applied to various developmental stages of Schistosoma mansoni. An 18 kDa protein was preferentially labeled in freshly transformed schistosomula. The labeled protein was equally present on skin-penetrated and mechanically prepared schistosomula and it disappeared upon digestion o...

journal_title：Molecular and biochemical parasitology

authors： Liberti P,Festucci A,Ruppel A,Gigante S,Cioli D

更新日期：1986-01-01 00:00:00

abstract：:The usefulness of random amplified polymorphic DNA markers (RAPD) was assayed in an attempt to discriminate among species, strains and individuals within the genus Schistosoma. Depending on the species, 40-50 arbitrary decamer oligonucleotides were used as primers to amplify total DNA by the polymerase chain reaction ...

journal_title：Molecular and biochemical parasitology

authors： Barral V,This P,Imbert-Establet D,Combes C,Delseny M

更新日期：1993-06-01 00:00:00

abstract：:Release of sporozoites from Eimeria oocysts/sporocysts is an essential step in the intracellular development of the parasite in its host. Little is known about this process except that elevated temperature (∼ 40 °C) plus trypsin and bile salts are required for sporozoite to escape from sporocysts. In this study, it wa...

journal_title：Molecular and biochemical parasitology

authors： Jenkins MC,O'Brien CN,Parker C

更新日期：2019-10-01 00:00:00

abstract：:Plasmodium falciparum is the causative organism for the most severe form of malaria among humans. The clinical symptoms are accredited to the asexual stage of parasite life cycle, involving merozoite invasion of erythrocyte, development and re-invasion into the new erythrocyte. Interaction of parasite proteins present...

journal_title：Molecular and biochemical parasitology

authors： Hans N,Relan U,Dubey N,Gaur D,Chauhan VS

更新日期：2015-08-01 00:00:00

abstract：:Nearly all mitochondrial RNA polymerase genes identified to date are encoded in the nucleus and have similarities to T3 and T7 bacteriophage RNA polymerases. Some chloroplast genes are also transcribed by T3/T7 phage-like RNA polymerases, raising the possibility that the apicomplexan parasites, which have both a mitoc...

journal_title：Molecular and biochemical parasitology

authors： Li J,Maga JA,Cermakian N,Cedergren R,Feagin JE

更新日期：2001-04-06 00:00:00

abstract：:Intraerythrocytic Plasmodium falciparum digests vast amounts of hemoglobin within an acidic food vacuole (FV). Four homologous aspartic proteases participate in hemoglobin degradation within the FV. Plasmepsin (PM) I and II are thought to initiate degradation of the native hemoglobin molecule. PM IV and histo-aspartic...

journal_title：Molecular and biochemical parasitology

authors： Banerjee R,Francis SE,Goldberg DE

更新日期：2003-07-01 00:00:00

abstract：:Profilins are the key regulators of actin dynamics in all eukaryotic cells. However, little information is available on their biochemical properties and functions in kinetoplastids, such as Trypanosoma and Leishmania. We show here that Leishmania parasites express only one homolog of profilin (LdPfn), which catalyzes ...

journal_title：Molecular and biochemical parasitology

authors： Ambaru B,Gopalsamy A,Tammana TVS,Subramanya HS,Gupta CM

更新日期：2020-07-01 00:00:00

abstract：:The azole antifungals ketoconazole and itraconazole possess in vitro antileishmanial activity against Leishmania mexicana mexicana amastigotes in macrophages (cell line J774G8). As in yeast and fungi, the activity is likely to be due to inhibition of the cytochrome P-450-dependent 14 alpha-demethylation of lanosterol ...

journal_title：Molecular and biochemical parasitology

authors： Hart DT,Lauwers WJ,Willemsens G,Vanden Bossche H,Opperdoes FR

更新日期：1989-03-01 00:00:00

abstract：:The origin of membraneous structures in the cytoplasm of human erythrocytes infected with the malaria parasite, Plasmodium falciparum, was determined by confocal fluorescence imaging microscopy. When infectious merozoites invaded erythrocytes labeled with the fluorescent, lipophilic, non-exchangeable molecules DiIC16 ...

journal_title：Molecular and biochemical parasitology

authors： Pouvelle B,Gormley JA,Taraschi TF

更新日期：1994-07-01 00:00:00

abstract：:The physiological role of Schistosomiasis mansoni 22.6 antigen (sm22.6 Ag) and its pathogenic effect on the human host has never been reported. Recombinant sm22.6 Ag is a homogenous polymer under non-denaturing/non-reducing conditions, and an inhibitor to human thrombin. Kinetic and Western blot assays show that the r...

journal_title：Molecular and biochemical parasitology

authors： Lin YL,He S

更新日期：2006-05-01 00:00:00

abstract：:Acetylcholinesterase (AChE) plays a key role in postsynaptic transmission in most animals. Nematodes encode multiple AChEs, implying its functional diversity. To explore physiological functions of multiple AChEs, three distinct AChEs (BxACE-1, BxACE-2, and BxACE-3) were identified and characterized from the pinewood n...

journal_title：Molecular and biochemical parasitology

authors： Kang JS,Lee DW,Choi JY,Je YH,Koh YH,Lee SH

更新日期：2011-02-01 00:00:00

abstract：:Trophozoites of Entamoeba invadens were able to ingest glucopolysaccharides and metabolize them. An activity capable of degrading these substrates was purified to apparent homogeneity. The enzyme was identified as beta-amylase (alpha-1,4-D-glucan maltohydrolase EC 3.2.1.2): It was active against glycogen, amylose and ...

journal_title：Molecular and biochemical parasitology

authors： Serafimova F,Franz A,Werries E

更新日期：1996-12-20 00:00:00

abstract：:Many surface antigens of the human malaria parasite Plasmodium falciparum show extraordinary diversity, with different alleles being so divergent as to be unalignable in some coding regions. To better understand the population history and modes of selection on such loci, we sequenced genomic regions flanking the highl...

journal_title：Molecular and biochemical parasitology

authors： Amodu OK,Hartl DL,Roy SW

更新日期：2008-05-01 00:00:00

abstract：:Beta 1,3-glucan recognition proteins (GRP) have specific affinity for beta 1,3-glucan, a component on the surface of fungi and bacteria. By interacting with beta 1,3-glucan, GRP initiates activation of prophenoloxidase, a key enzyme in the signaling pathway leading to melanotic encapsulation in invertebrates. In this ...

journal_title：Molecular and biochemical parasitology

authors： Wang X,Fuchs JF,Infanger LC,Rocheleau TA,Hillyer JF,Chen CC,Christensen BM

更新日期：2005-01-01 00:00:00

abstract：:Phosphoserine phosphatase (PSP) catalyzes the third step of the phosphorylated serine biosynthetic pathway, and occurred multiple times in evolution, while enzymes catalyzing the first and second steps in the pathway have single respective origins. In the present study, we examined the existence of PSP among genus Ent...

journal_title：Molecular and biochemical parasitology

authors： Chiba Y,Makiuchi T,Jeelani G,Nozaki T

更新日期：2016-06-01 00:00:00

abstract：:A family of Schistosoma mansoni proteins (18-22 kDa, pI 5.3-5.8) are biosynthesized in juvenile worms and immunoprecipitated by antibodies uniquely present in protective Fischer rat antiserum. A cDNA clone, lambda gt11-40, expressing epitopes common to this protein family was used to obtain a genomic DNA clone, by hyb...

journal_title：Molecular and biochemical parasitology

authors： el-Sherbeini M,Ramadan N,Bostian KA,Knopf PM

更新日期：1991-11-01 00:00:00

abstract：:Toxoplasma gondii differentially expresses two forms of lactate dehydrogenase in tachyzoites and bradyzoites, respectively, designated LDH1 and LDH2. Previously it was demonstrated that LDH1 and LDH2 share a unique structural feature with LDH from the malarial parasite Plasmodium falciparum (pLDH), namely, the additio...

journal_title：Molecular and biochemical parasitology

authors： Dando C,Schroeder ER,Hunsaker LA,Deck LM,Royer RE,Zhou X,Parmley SF,Vander Jagt DL

更新日期：2001-11-01 00:00:00

abstract：:Unequal usage of synonymous codons in the gene transcript for an amino acid is known as codon usage bias (CUB). It is a unique property of gene as well as genome. Mutation and natural selection are known to be the major factors that influence CUB. Other factors encompass gene expression level, GC content, codon positi...

journal_title：Molecular and biochemical parasitology

authors： Mazumder GA,Uddin A,Chakraborty S

更新日期：2018-10-01 00:00:00

abstract：:Even at the present age of whole-organism analysis, e.g., genomics, transcriptomics, and proteomics, the biological roles of many proteins remain unresolved. Classified among the proteins of unknown function is a family of proteins harboring repeats of the DM9 domain, a 60-75 amino acids motif first described in a sma...

journal_title：Molecular and biochemical parasitology

authors： Phadungsil W,Smooker PM,Vichasri-Grams S,Grams R

更新日期：2016-01-01 00:00:00

abstract：:The apicomplexan Toxoplasma gondii replicates by endodyogeny, in which replicated organelles assemble into nascent daughter buds within the maternal parasite. The mechanisms governing this complex sequence are not understood. We now report that the kinase inhibitor 3-methlyadenine (3-MA) efficiently blocks T. gondii r...

journal_title：Molecular and biochemical parasitology

authors： Wang Y,Karnataki A,Parsons M,Weiss LM,Orlofsky A

更新日期：2010-10-01 00:00:00

abstract：:During intracellular development of the malarial parasite numerous membranous vesicles appear in the infected erythrocyte cytoplasm between the parasitophorous vacuolar membrane (PVM) and the erythrocyte plasma membrane. In this study we describe the characterization of a monoclonal antibody which recognizes two major...

journal_title：Molecular and biochemical parasitology

authors： Martinez SL,Clavijo CA,Winograd E

更新日期：1998-03-15 00:00:00

abstract：:Phosphatidylcholine (PC) is the most abundant phospholipid in the membranes of the human parasite Leishmania. It is synthesized via two metabolic routes, the de novo pathway that starts with the uptake of choline, and the threefold methylation of phosphatidylethanolamine. Choline was shown to be dispensable for Leishm...

journal_title：Molecular and biochemical parasitology

authors： Bibis SS,Dahlstrom K,Zhu T,Zufferey R

更新日期：2014-09-01 00:00:00

abstract：:The capacity for rapid localization of epitope-tagged or fluorescent fusion proteins in cells is an important tool for biological discovery and functional analysis. For Trypanosoma cruzi, the protozoan parasite that causes human Chagas disease, visualization of ectopically-expressed proteins in the clinically-relevant...

journal_title：Molecular and biochemical parasitology

authors： Padmanabhan PK,Polidoro RB,Barteneva NS,Gazzinelli RT,Burleigh BA

更新日期：2014-12-01 00:00:00

abstract：:Cathepsin L-like activity was demonstrated in the excretory/secretory (E/S) products of Fasciola hepatica newly excysted juveniles (NEJ), 3-week-old, 5-week-old and mature flukes using the fluorogenic substituted 7-amino-4-methylcoumarin substrates Z-phe-arg-AMC, Z-arg-arg-AMC and Z-arg-AMC. Gelatin-substrate polyacry...

journal_title：Molecular and biochemical parasitology

authors： Carmona C,Dowd AJ,Smith AM,Dalton JP

更新日期：1993-11-01 00:00:00

abstract：:The monosaccharide composition of an affinity-purified family of antigenically-related Trichinella spiralis larval glycoproteins was determined by gas chromatography/mass spectrometry. This group of 6 major glycoproteins, designated TSL-1, originates in the muscle stage (L1) larval stichosome. They are present on the ...

journal_title：Molecular and biochemical parasitology

authors： Wisnewski N,McNeil M,Grieve RB,Wassom DL

更新日期：1993-09-01 00:00:00

abstract：:We have compared a total of 44 recognition sites for 12 restriction endonucleases on the 20 kilobase pair maxi-circle of kinetoplast DNA from nine Trypanosoma brucei stocks, four which are known to be infective to man (tow 'gambiense' and two 'rhodesiense' variants). In addition to five polymorphic sites, these DNAs d...

journal_title：Molecular and biochemical parasitology

authors： Borst P,Fase-Fowler F,Gibson WC

更新日期：1981-06-01 00:00:00

abstract：:A Fasciola hepatica cDNA clone of 1752 bp was isolated from an adult worm cDNA expression library by immunological screening using a rabbit serum against the excretory-secretory antigens. The nucleotide sequence of the cDNA revealed the presence of an open reading frame of 489 codons which encoded a 55 kDa polypeptide...

journal_title：Molecular and biochemical parasitology

authors： Salazar-Calderón M,Martín-Alonso JM,Castro AM,Parra F

更新日期：2003-01-01 00:00:00

abstract：:Leishmania major aquaglyceroporin (AQP1) is an adventitious metalloid channel that allows the bidirectional movement of arsenite and antimonite. Here we demonstrate that AQP1 is subjected to proteasome-dependent degradation. Treatment of Leishmania promastigotes with the proteasome inhibitor MG132 resulted in increase...

journal_title：Molecular and biochemical parasitology

authors： Sharma M,Mandal G,Mandal S,Bhattacharjee H,Mukhopadhyay R

更新日期：2015-06-01 00:00:00

abstract：:Cryptosporidium spp. are intracellular apicomplexan parasites that cause outbreaks of waterborne diarrheal disease worldwide. Previous studies had identified a Cryptosporidium parvum sporozoite antigen, CpMuc4, that appeared to be involved in attachment and invasion of the parasite into intestinal epithelial cells. Cp...

journal_title：Molecular and biochemical parasitology

authors： Paluszynski J,Monahan Z,Williams M,Lai O,Morris C,Burns P,O'Connor R

更新日期：2014-02-01 00:00:00