Characterization of trafficking pathways and membrane genesis in malaria-infected erythrocytes.


:The origin of membraneous structures in the cytoplasm of human erythrocytes infected with the malaria parasite, Plasmodium falciparum, was determined by confocal fluorescence imaging microscopy. When infectious merozoites invaded erythrocytes labeled with the fluorescent, lipophilic, non-exchangeable molecules DiIC16 or DiOC16, a ring of fluorescence was observed surrounding the internal parasite, indicating that the parasitophorous vacuolar membrane (PVM) is formed in part from the erythrocyte membrane. As the parasites matured, fluorescent vesicles were seen to be exported into the erythrocyte cytoplasm, beginning at 6 h post-invasion. During intraerythrocytic development, these dyes were transferred from the PVM to the parasite. When fluorescently labeled merozoites were released from these cells and invaded unlabeled erythrocytes, fluorescence was confined to the parasite throughout the entire erythrocytic cycle. Taken together, these results demonstrate that all vesicles/membranous compartments in the erythrocyte cytoplasm of parasitized erythrocytes (IRBC) contain membrane derived from the PVM. Based on this information, we define pathways that the parasite utilizes to export proteins and lipids to the host cell cytoplasm and surface membrane. When IRBC were labeled post-invasion with DiIC16 or DiOC16 and the parasites allowed to mature for one life cycle, the dyes were confined to the erythrocyte membrane, demonstrating that the host cell membrane of IRBC does not endocytose and there is no membrane exchange from the erythrocyte to the parasite. This investigation helps to resolve two long-standing controversies and provides new insights into the transport pathways that malaria parasites utilize during their development within host erythrocytes.


Mol Biochem Parasitol


Pouvelle B,Gormley JA,Taraschi TF




Has Abstract


1994-07-01 00:00:00














  • Isolation and partial sequence of a collagen gene from the human filarial parasite Brugia malayi.

    abstract::We report the isolation and sequence of a part of a gene encoding a collagen from the genome of the human filarial parasite Brugia malayi. The deduced amino acid sequence of the sequenced portion of this gene, which we have designated BmCol1, differs from the most catalogued nematode collagens in that it is composed p...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Caulagi VR,Werner C,Rajan TV

    更新日期:1991-03-01 00:00:00

  • Stage independent chloroquine resistance and chloroquine toxicity revealed via spinning disk confocal microscopy.

    abstract::We previously customized a Nipkow spinning disk confocal microscope (SDCM) to acquire 4D data for live, intraerythrocytic malarial parasites [Gligorijevic B, McAllister R, Urbach JS, Roepe, PD. Spinning disk confocal microscopy of live, intraerythrocytic malarial parasites. 1. Quantification of hemozoin development fo...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Gligorijevic B,Purdy K,Elliott DA,Cooper RA,Roepe PD

    更新日期:2008-05-01 00:00:00

  • Enzymes of purine and pyrimidine metabolism from the human malaria parasite, Plasmodium falciparum.

    abstract::Plasmodium falciparum trophozoites were isolated by mechanical rupture of infected human erythrocytes followed by a series of differential centrifugation steps. After lysis with sonication, the 100 000 x g supernatant of parasites and uninfected host cells was used to determine the specific activities of a number of e...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Reyes P,Rathod PK,Sanchez DJ,Mrema JE,Rieckmann KH,Heidrich HG

    更新日期:1982-05-01 00:00:00

  • Protoporphyrinogen IX oxidase from Plasmodium falciparum is anaerobic and is localized to the mitochondrion.

    abstract::Earlier studies in this laboratory had shown that the malarial parasite can synthesize heme de novo and inhibition of the pathway leads to death of the parasite. It has been proposed that the pathway for the biosynthesis of heme in Plasmodium falciparum is unique involving three different cellular compartments, namely...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Nagaraj VA,Arumugam R,Prasad D,Rangarajan PN,Padmanaban G

    更新日期:2010-11-01 00:00:00

  • The merozoite surface protein 6 gene codes for a 36 kDa protein associated with the Plasmodium falciparum merozoite surface protein-1 complex.

    abstract::A complex of non-covalently bound polypeptides is located on the surface of the merozoite form of the human malaria parasite Plasmodium falciparum. Four of these polypeptides are derived by proteolytic processing of the merozoite surface protein 1 (MSP-1) precursor. Two components, a 22 and a 36 kDa polypeptide are no...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Trucco C,Fernandez-Reyes D,Howell S,Stafford WH,Scott-Finnigan TJ,Grainger M,Ogun SA,Taylor WR,Holder AA

    更新日期:2001-01-15 00:00:00

  • Isolation of lambda amp3 genomic recombinants coding for antigens of Eimeria tenella.

    abstract::Eimeria are the causative agents of coccidosis, a disease which is of world wide economic importance in the poultry industry. Immunity resulting from infection is species-specific and both antibody and cell-mediated responses have been implicated. As an initial step in the development of a genetically-engineered vacci...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Clarke LE,Messer LI,Greenwood NM,Wisher MH

    更新日期:1987-01-02 00:00:00

  • Molecular basis of binding of the Plasmodium falciparum receptor BAEBL to erythrocyte receptor glycophorin C.

    abstract::Plasmodium falciparum invades human erythrocytes by redundant pathways. Unlike Plasmodium vivax that has one Duffy Binding-Like (DBL) receptor, P. falciparum has four members of the DBL receptor family. Furthermore, one of these DBL genes, BAEBL, has polymorphisms at four amino acids in region II; each polymorphism bi...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Jiang L,Duriseti S,Sun P,Miller LH

    更新日期:2009-11-01 00:00:00

  • Translation initiation factors GleIF4E2 and GleIF4A can interact directly with the components of the pre-initiation complex to facilitate translation initiation in Giardia lamblia.

    abstract::Translation initiation factor eIF4F is essential for cap-dependent translation initiation in eukaryotes. eIF4F is a trimeric complex consisting of a scaffold protein eIF4G, cap-binding protein eIF4E and DEAD-box RNA helicase eIF4A. eIF4F binds to the 5' cap structure of the mRNA through eIF4E and facilitates the bindi...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Adedoja AN,McMahan T,Neal JP,Hamal Dhakal S,Jois S,Romo D,Hull K,Garlapati S

    更新日期:2020-03-01 00:00:00

  • Identification of immunoreactive proteins from the dog heartworm (Dirofilaria immitis) differentially recognized by the sera from dogs with patent or occult infections.

    abstract::Heartworm disease caused by Dirofilaria immitis affects canine and feline hosts. Moreover, the parasite can infect humans, causing pulmonary dirofilariosis. Most affected dogs have patent infections with circulating microfilariae in peripheral blood, although infected dogs sometimes develop occult infections character...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Oleaga A,Pérez-Sánchez R,Pagés E,Marcos-Atxutegi C,Simón F

    更新日期:2009-08-01 00:00:00

  • Molecular cloning and nuclear localization of a histone deacetylase homologue in Plasmodium falciparum.

    abstract::Reversible acetylation of core histones plays an important role in transcriptional regulation, cell cycle progression and developmental events. The acetylation state of histones is controlled by a dynamic equilibrium between activities of histone acetylase and deacetylase enzymes. Histone deacetylase (HDAC) was recent...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Joshi MB,Lin DT,Chiang PH,Goldman ND,Fujioka H,Aikawa M,Syin C

    更新日期:1999-03-15 00:00:00

  • Development of an untargeted metabolomics method for the analysis of human faecal samples using Cryptosporidium-infected samples.

    abstract::Faecal metabolite profiling, though in its infancy, allows for investigation of complex metabolic interactions between gastrointestinal infections or diseases and host health. In the present study, we describe a faecal metabolite extraction method for untargeted gas chromatography-mass spectrometry (GC-MS) analysis us...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ng JS,Ryan U,Trengove RD,Maker GL

    更新日期:2012-10-01 00:00:00

  • Electron transfer complexes of Ascaris suum muscle mitochondria: I. Characterization of NADH-cytochrome c reductase (complex I-III), with special reference to cytochrome localization.

    abstract::An NADH-cytochrome c reductase (complex I-III) was isolated from Ascaris suum muscle mitochondria. The enzyme preparation catalyzed the reduction of 1.68 mumol cytochrome c min-1 mg-1 protein at 25 degrees C with NADH but not with NADPH, and retained its sensitivity to rotenone, piericidin A and 2-heptyl-4-hydroxyquin...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Takamiya S,Furushima R,Oya H

    更新日期:1984-10-01 00:00:00

  • Purification and characterization of gamma-glutamylcysteine synthetase from Ascaris suum.

    abstract::We have purified and characterized the Ascaris suum gamma-glutamylcysteine synthetase, the rate-limiting step in the glutathione biosynthesis. The purified enzyme exhibited a specific activity of 18 U (mg protein)-1. Estimation of the molecular mass of the native enzyme by FPLC on Superdex S-200 revealed the presence ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hussein AS,Walter RD

    更新日期:1995-06-01 00:00:00

  • Sequencing and characterization of an Echinococcus multilocularis DNA probe and its use in the polymerase chain reaction.

    abstract::The nucleotide sequence of the cloned Echinococcus multilocularis DNA probe pAL1 was determined in order to simplify and improve the sensitivity of a diagnostic assay through the application of the polymerase chain reaction (PCR). The insert-specific oligonucleotides BG1 and BG2 define a 2.6-kb fragment in the genomic...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Gottstein B,Mowatt MR

    更新日期:1991-02-01 00:00:00

  • Molecular characterization of a thrombospondin-related anonymous protein homologue in Neospora caninum.

    abstract::Thrombospondin-related anonymous protein (TRAP) family members participate in attachment and invasion of host cells by apicomplexan parasites. A TRAP homologue in Neospora caninum strain Nc-1 (NcMIC2) was cloned, sequenced and found to be 61% identical (75% similar) at the amino acid level to Toxoplasma gondii MIC2 (T...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Lovett JL,Howe DK,Sibley LD

    更新日期:2000-03-15 00:00:00

  • Molecular cloning of a myoD-like gene from the parasitic nematode, Trichinella spiralis.

    abstract::The infective larval stage of the nematode Trichinella spiralis is an intracellular parasite of skeletal muscle cells. Infection with T. spiralis results in dedifferentiation of the host cell and the formation of a host/parasite complex. A gene encoding a T. spiralis Helix-Loop-helix (HLH) protein with homology to the...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Connolly B,Trenholme K,Smith DF

    更新日期:1996-10-30 00:00:00

  • Proteolytic activity of Plasmodium falciparum subtilisin-like protease 3 on parasite profilin, a multifunctional protein.

    abstract::Subtilisin-like proteases of malaria parasite Plasmodium falciparum (PfSUB1, 2 and 3) are expressed at late asexual blood stages. PfSUB1 and 2 are considered important drug targets due to their essentiality for parasite blood stages and role in merozoite egress and invasion of erythrocytes. We have earlier shown the i...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Alam A,Bhatnagar RK,Relan U,Mukherjee P,Chauhan VS

    更新日期:2013-10-01 00:00:00

  • Quantitation of genetic differences between Trypanosoma brucei gambiense, rhodesiense and brucei by restriction enzyme analysis of kinetoplast DNA.

    abstract::We have compared a total of 44 recognition sites for 12 restriction endonucleases on the 20 kilobase pair maxi-circle of kinetoplast DNA from nine Trypanosoma brucei stocks, four which are known to be infective to man (tow 'gambiense' and two 'rhodesiense' variants). In addition to five polymorphic sites, these DNAs d...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Borst P,Fase-Fowler F,Gibson WC

    更新日期:1981-06-01 00:00:00

  • Tests of cytoplasmic RNA interference (RNAi) and construction of a tetracycline-inducible T7 promoter system in Trypanosoma cruzi.

    abstract::The technique of RNA interference (RNAi) is exceedingly useful for knocking down the expression of a specific mRNA in African trypanosomes and other organisms for the purpose of examining the function of its gene. However, when we attempted to apply RNAi in the Latin American trypanosome, Trypanosoma cruzi, to diminis...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: DaRocha WD,Otsu K,Teixeira SM,Donelson JE

    更新日期:2004-02-01 00:00:00

  • Genomic organisation of nuclear tRNAGly and tRNALeu genes in Trypanosoma brucei.

    abstract::We have isolated a 0.3-kb HaeIII restriction fragment from Trypanosoma brucei which contains two tRNA genes. Secondary structure models predict that the two genes identified encode tRNA molecules which specify glycine (anticodon UCC) and leucine (anticodon CAG). The two genes are separated by 86 nucleotides, transcrib...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Campbell DA,Suyama Y,Simpson L

    更新日期:1989-12-01 00:00:00

  • Molecular cloning and analysis of Ancylostoma ceylanicum glutamate-cysteine ligase.

    abstract::Glutamate-cysteine ligase (GCL) is a heterodimer enzyme composed of a catalytic subunit (GCLC) and a modifier subunit (GCLM). This enzyme catalyses the synthesis of γ-glutamylcysteine, a precursor of glutathione. cDNAs of the putative glutamate-cysteine ligase catalytic (Ace-GCLC) and modifier subunits (Ace-GCLM) of A...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Wiśniewski M,Lapiński M,Zdziarska A,Długosz E,Bąska P

    更新日期:2014-08-01 00:00:00

  • Zinc finger nuclease technology: A stable tool for high efficiency transformation in bloodstream form T. brucei.

    abstract::In Trypanosoma brucei, the generation of knockout mutants is relatively easy compared to other organisms as transfection methods are well established. These methods have their limitations, however, when it comes to the generation of genome-wide libraries that require a minimum of several hundred thousand transformants...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Schumann G,Kangussu-Marcolino MM,Doiron N,Käser S,de Assis Burle-Caldas G,DaRocha WD,Teixeira SM,Roditi I

    更新日期:2017-04-01 00:00:00

  • Isolation and functional characterization of snail hemocyte-modulating polypeptide from primary sporocysts of Schistosoma mansoni.

    abstract::Infection with larval trematodes has been shown to inhibit several snail-host defences, including hemocyte phagocytosis, cytotoxicity, motility, and adherence. Certain plasma factors which mediate snail defence responses, and which may be produced by host hemocytes, also appear to be altered by these parasites. In thi...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Lodes MJ,Connors VA,Yoshino TP

    更新日期:1991-11-01 00:00:00

  • The genomic organization of guide RNA genes in kinetoplastid protozoa: several conundrums and their solutions.

    abstract::The guide RNA (gRNA) paradigm states that the uridine (U) insertion/deletion type of RNA editing is mediated by short 3' uridylylated gRNAs that are complementary to specific blocks of mature edited sequence. These gRNAs contain the edited sequence information in the form of guiding purine residues that can base pair ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Simpson L

    更新日期:1997-06-01 00:00:00

  • A comparison of the enzymatic properties of the major cysteine proteinases from Trypanosoma congolense and Trypanosoma cruzi.

    abstract::Congopain and cruzipain, the major cysteine proteinases from Trypanosoma congolense and Trypanosoma cruzi, were compared for their activities towards a series of new, sensitive fluorogenic substrates of the papain family of cysteine proteinases and for their sensitivity to inhibition by cystatins and related biotinyla...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Chagas JR,Authie E,Serveau C,Lalmanach G,Juliano L,Gauthier F

    更新日期:1997-09-01 00:00:00

  • Organization, sequence and stage-specific expression of the phosphoglycerate kinase genes of Leishmania mexicana mexicana.

    abstract::In Leishmania mexicana two genes were detected coding for different isoforms of the glycolytic enzyme phosphoglycerate kinase. This situation contrasts with that observed in other Trypanosomatidae (Trypanosoma brucei, Trypanosoma congolense, Crithidia fasciculata) analyzed previously, which all contain three different...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Adjé CA,Opperdoes FR,Michels PA

    更新日期:1997-12-01 00:00:00

  • The influence of temperature and serum deprivation on the synthesis of heat-shock proteins and alpha and beta tubulin in promastigotes of Leishmania major.

    abstract::We have examined changes in the relative synthesis of individual proteins in promastigotes of Leishmania major subjected to decreasing serum levels in vitro. We observed increases in the relative synthesis of the putative heat-shock proteins of 82 and 70 kDa and of proteins of 79 and 41 kDa but decreases in the synthe...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Toye P,Remold H

    更新日期:1989-06-01 00:00:00

  • Biochemical and functional characterization of CpMuc4, a Cryptosporidium surface antigen that binds to host epithelial cells.

    abstract::Cryptosporidium spp. are intracellular apicomplexan parasites that cause outbreaks of waterborne diarrheal disease worldwide. Previous studies had identified a Cryptosporidium parvum sporozoite antigen, CpMuc4, that appeared to be involved in attachment and invasion of the parasite into intestinal epithelial cells. Cp...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Paluszynski J,Monahan Z,Williams M,Lai O,Morris C,Burns P,O'Connor R

    更新日期:2014-02-01 00:00:00

  • ABC transporters in the liver fluke Opisthorchis felineus.

    abstract::ATP-binding cassette transporters (ABC transporters) are essential components of normal cellular physiological machinery in all eukaryotic and prokaryotic species, including parasites. Some ABC transporters, e.g., P-glycoproteins, are involved in the efflux of toxins and xenobiotics from the cell. At present, nothing ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Mordvinov VA,Ershov NI,Pirozhkova DS,Pakharukov YV,Pakharukova MY

    更新日期:2017-09-01 00:00:00

  • Expression of foreign proteins in Trypanosoma congolense.

    abstract::An expression vector was constructed to express foreign genes in Trypanosoma congolense. The foreign gene and a neomycin phosphotransferase (NPT) gene are flanked by glutamate and alanine rich protein (GARP) gene processing signals and their expression is driven by a ribosomal RNA gene promoter. The plasmid is not mai...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Downey N,Donelson JE

    更新日期:1999-10-25 00:00:00