High efficiency transfection of Plasmodium berghei facilitates novel selection procedures.

abstract：:Cytoadherence of infected erythrocytes is a hallmark of Plasmodium falciparum infection and a key determinant in the particular virulence of this species. Infected erythrocytes bind a variety of host receptors but certain adhesion traits are associated with more severe disease. A large, diverse protein family named P....

journal_title：Molecular and biochemical parasitology

authors： Kraemer SM,Gupta L,Smith JD

更新日期：2003-06-01 00:00:00

abstract：:An immunodominant 72-kDa surface glycoprotein (Gp72) of Trypanosoma cruzi is involved in adhesion of the flagellum to the cell body (Cooper, R, Ribeiro de Jesus, A and Cross, G.A.M (1993) J. Cell Biol. 122, 149-156). We have characterized a gene, flagellum-adhesion glycoprotein genel (fla1), from Trypanosoma brucei th...

journal_title：Molecular and biochemical parasitology

authors： Nozaki T,Haynes PA,Cross GA

更新日期：1996-11-25 00:00:00

abstract：:We previously customized a Nipkow spinning disk confocal microscope (SDCM) to acquire 4D data for live, intraerythrocytic malarial parasites [Gligorijevic B, McAllister R, Urbach JS, Roepe, PD. Spinning disk confocal microscopy of live, intraerythrocytic malarial parasites. 1. Quantification of hemozoin development fo...

journal_title：Molecular and biochemical parasitology

authors： Gligorijevic B,Purdy K,Elliott DA,Cooper RA,Roepe PD

更新日期：2008-05-01 00:00:00

abstract：:A polyprotein composed of multiple units arranged in direct tandem arrays has been identified in parasitic and free living nematodes. Analysis of previously cloned units from the Dirofilaria immitis polyprotein antigen (DiPA) indicated the units were nearly identical but here we demonstrate that they segregate into tw...

journal_title：Molecular and biochemical parasitology

authors： Poole CB,Hornstra LJ,Benner JS,Fink JR,McReynolds LA

更新日期：1996-11-12 00:00:00

abstract：:Trichomonas vaginalis is a parasitic protozoan of both medical and biological relevance. Transcriptional studies in this organism have focused mainly on type II pol promoters, whereas the elements necessary for transcription by polI or polIII have not been investigated. Here, with the aid of a transient transcription ...

journal_title：Molecular and biochemical parasitology

authors： Franco B,Hernández R,López-Villaseñor I

更新日期：2012-09-01 00:00:00

abstract：:Adult Schistosoma mansoni worms rapidly degrade their endogenous glycogen stores immediately after isolation from the host. In NCTC 109 or in a diphasic culture medium the glycogen levels slowly recovered again after the initial decrease. The rapid degradation of glycogen could be prevented, even in a simple salt medi...

journal_title：Molecular and biochemical parasitology

authors： Tielens AG,van den Bergh SG

更新日期：1987-07-01 00:00:00

abstract：:We have determined the sequence of a cDNA (Tt34c1) encoding a Trypanosoma cruzi trypomastigote stage-specific 85-kDa surface glycoprotein (gp85). Within the peptide sequence of Tt34c1 are two 8-amino acid motifs, Ser-X-Asp-X-Gly-X-Thr-Trp, that are characteristic of bacterial neuraminidases. Analysis of the Tt34c1 seq...

journal_title：Molecular and biochemical parasitology

authors： Takle GB,Cross GA

更新日期：1991-10-01 00:00:00

abstract：:A total of 518 expressed sequence tags (ESTs) have been generated from clones randomly selected from a cDNA library and a spliced leader sub-library of a Trypanosoma brucei rhodesiense bloodstream clone. 205 (39%) of the clones were identified based on matches to 113 unique genes in the public databases. Of these, 71 ...

journal_title：Molecular and biochemical parasitology

authors： el-Sayed NM,Alarcon CM,Beck JC,Sheffield VC,Donelson JE

更新日期：1995-07-01 00:00:00

abstract：:Malaria transmission occurs during a blood-meal of an infected Anopheles mosquito. Visualization and quantification of sporozoites along the journey from the mosquito midgut, where they develop, to the vertebrate liver, their final target organ, is important for understanding many aspects of sporozoite biology. Here w...

journal_title：Molecular and biochemical parasitology

authors： Engelmann S,Sinnis P,Matuschewski K

更新日期：2006-03-01 00:00:00

abstract：:Both patent and prepatent adult Hymenolepis diminuta excreted 20-hydroxyecdysone into the culture medium when maintained in vitro. Patent worms also excreted ecdysone and comparatively large quantities of unidentified immunoreactive material of a relatively apolar nature. This latter material was shown to be depleted ...

journal_title：Molecular and biochemical parasitology

authors： Mercer JG,Munn AE,Arme C,Rees HH

更新日期：1987-12-01 00:00:00

abstract：:Proteins in malaria parasites (Plasmodium falciparum) isolated from a patient in Thailand before treatment, and after recrudescence of infection subsequent to mefloquine treatment, were compared by two dimensional polyacrylamide gel electrophoresis (2D-PAGE) analysis. Nine 'pre-treatment' and six 'recrudescent' clones...

journal_title：Molecular and biochemical parasitology

authors： Pinswasdi C,Thaithong S,Beale GH,Fenton B,Webster HK,Pavanand K

更新日期：1987-03-01 00:00:00

abstract：:Whilst the schistosome tegument has been intensively studied there is little information about processes in the gut, the other major interface with the bloodstream, apart from the well characterised cascade of proteases involved in haemoglobin digestion. To gain insights into gut function we undertook a proteomic anal...

journal_title：Molecular and biochemical parasitology

authors： Hall SL,Braschi S,Truscott M,Mathieson W,Cesari IM,Wilson RA

更新日期：2011-09-01 00:00:00

abstract：:The complex life cycles of many protozoan parasites require the ability to respond to environmental and developmental cues through regulated gene expression. Traditionally, parasitologists have investigated these mechanisms by identifying and characterizing proteins that are necessary for the regulated expression of t...

journal_title：Molecular and biochemical parasitology

authors： Militello KT,Refour P,Comeaux CA,Duraisingh MT

更新日期：2008-02-01 00:00:00

abstract：:gamma-Glutamyl transpeptidase, which is of central importance in the degradation of glutathione, was purified from Ascaris suum to apparent homogeneity. The enzyme was found to be a predominantly membrane-bound protein and was solubilized by Triton X-100. The purified enzyme, which exhibits a specific activity of 1009...

journal_title：Molecular and biochemical parasitology

authors： Hussein AS,Walter RD

更新日期：1996-04-01 00:00:00

abstract：:Parasitic infections are a widespread health problem and research of novel anthelmintic compounds is of the utmost importance. In this study we performed a virtual screening campaign by coupling ligand-based pharmacophore, homology modeling and molecular docking. The virtual screening campaign was conducted using a jo...

journal_title：Molecular and biochemical parasitology

authors： Stevanovic S,Marjanović DS,Trailović SM,Zdravković N,Perdih A,Nikolic K

更新日期：2021-01-07 00:00:00

abstract：:TgPrx2 represents a recently discovered cytosolic 1-Cys peroxiredoxin (Prx) from the intracellular parasite Toxoplasma gondii. Over-expression of the respective gene confers protection against H(2)O(2), suggesting that the protein possesses peroxidase activity. According to the current nomenclature eukaryotic typical ...

journal_title：Molecular and biochemical parasitology

authors： Deponte M,Becker K

更新日期：2005-03-01 00:00:00

abstract：:Ribosomal RNA isolated from the malaria parasite Plasmodium falciparum consists of two species with molecular weight of 1.49 +/- 0.09 X 10(6) and 0.78 +/- 0.02 X 10(6) present in equimolar quantities. Their molecular weights are comparable with those of other protozoa but quite distinct from those of the human host. T...

journal_title：Molecular and biochemical parasitology

authors： Hyde JE,Zolg JW,Scaife JG

更新日期：1981-12-31 00:00:00

abstract：:The Plasmodium falciparum Erythrocyte Binding Antigen-175, EBA-175, is a soluble merozoite stage parasite protein which binds to glycophorin A surface receptors on human erythrocytes. We have expressed two conserved cysteine-rich regions, region II and region VI, of this protein as soluble His-tagged polypeptides in i...

journal_title：Molecular and biochemical parasitology

authors： Ockenhouse CF,Barbosa A,Blackall DP,Murphy CI,Kashala O,Dutta S,Lanar DE,Daugherty JR

更新日期：2001-03-01 00:00:00

abstract：:In most organisms rDNA transcription ceases under conditions of growth stress. However, we have earlier shown that pre-rRNA accumulates during encystation in Entamoeba invadens. We labeled newly-synthesized rRNA during encystation, with [methyl-(3)H] methionine in the presence of chitinase to enable uptake of isotope....

journal_title：Molecular and biochemical parasitology

authors： Ojha S,Ahamad J,Bhattacharya A,Bhattacharya S

更新日期：2014-06-01 00:00:00

abstract：:The promastigote surface antigen 2 (PSA-2) complex comprises a family of antigenically similar polypeptides of M(r) 96,000, 80,000 and 50,000, anchored to the membrane with glycosylphosphatidylinositol. Although PSA-2 was initially detected only in promastigotes, Northern blot analysis indicated that mRNA transcripts ...

journal_title：Molecular and biochemical parasitology

authors： Handman E,Osborn AH,Symons F,van Driel R,Cappai R

更新日期：1995-11-01 00:00:00

abstract：:The issue of whether genetic exchange occurs at a significant frequency in natural populations of Trypanosoma brucei is controversial and one of the arguments against a high frequency has been the apparent lack of host infections with mixtures of trypanosome genotypes. Three minisatellite markers (MS42, CRAM, 292) wit...

journal_title：Molecular and biochemical parasitology

authors： MacLeod A,Turner CM,Tait A

更新日期：1999-08-20 00:00:00

abstract：:Antibodies were affinity purified from crude antiserum by elution from the 24 kDa region of preparative one-dimensional Western blots containing immobilized adult Schistosoma mansoni inner bilayer membrane proteins. They were shown to be specific for a single acidic polypeptide complex, Smgp24, following immunoblottin...

journal_title：Molecular and biochemical parasitology

authors： Karcz SR,Barnard BJ,Podesta RB

更新日期：1988-11-01 00:00:00

abstract：:The obligate intracellular protozoan parasite Toxoplasma gondii has a single tubular mitochondrion. During infection, it recruits the host cell's mitochondria abutting to the intracellular vacuole, that contains the parasites. The respective contribution of host and parasitic mitochondria in the intracellular growth o...

journal_title：Molecular and biochemical parasitology

authors： Toursel C,Dzierszinski F,Bernigaud A,Mortuaire M,Tomavo S

更新日期：2000-12-01 00:00:00

abstract：:It has been shown that vaccination with two low molecular mass excretory secretory (ES) antigens of 15 and 24 kDa, respectively, afforded a substantial degree of protection against Haemonchus contortus to sheep. In vitro cultivation of the parasite usually yields a limited amount of these proteins and therefore, recom...

journal_title：Molecular and biochemical parasitology

authors： Schallig HD,van Leeuwen MA,Verstrepen BE,Cornelissen AW

更新日期：1997-09-01 00:00:00

abstract：:Representatives of all major lineages of eukaryotes contain peroxisomes with similar morphology and mode of biogenesis, indicating a monophyletic origin of the organelles within the common ancestor of all eukaryotes. Peroxisomes originated from the endoplasmic reticulum, but despite a common origin and shared morpholo...

journal_title：Molecular and biochemical parasitology

authors： Gabaldón T,Ginger ML,Michels PA

更新日期：2016-01-01 00:00:00

abstract：:A 25-kb CpFAS1 gene from Cryptosporidium parvum has been engineered and expressed as five individual maltose-binding protein (MBP)-fusion proteins: an N-terminal loading unit, three fatty acyl elongation modules, and a C-terminal reductase. Enzymatic activities of all domains (except the reductase) were individually a...

journal_title：Molecular and biochemical parasitology

authors： Zhu G,Li Y,Cai X,Millership JJ,Marchewka MJ,Keithly JS

更新日期：2004-03-01 00:00:00

abstract：:This study further explores the effects of hypoxia and acute osmotic stress on intermediary metabolism of Leishmania major and Leishmania donovani. Late log phase promastigotes were washed and incubated with glucose as the sole exogenous carbon source, and rates of glucose consumption and product formation were measur...

journal_title：Molecular and biochemical parasitology

authors： Walsh MJ,Blum JJ

更新日期：1992-02-01 00:00:00

abstract：:Cysteine protease gene fragments from three protozoan parasites Trypanosoma cruzi, Trypanosoma brucei, and Entamoeba histolytica were amplified by the polymerase chain reaction (PCR) from genomic DNA using degenerate oligonucleotide primers. The primers used for the amplification were designed based upon amino acid se...

journal_title：Molecular and biochemical parasitology

authors： Eakin AE,Bouvier J,Sakanari JA,Craik CS,McKerrow JH

更新日期：1990-02-01 00:00:00

abstract：:Benznidazole is the main drug used to treat Trypanosoma cruzi infections. However, frequent instances of treatment failure have been reported. To better understand potential resistance mechanisms, we analysed three clones isolated from a single parasite population that had undergone benznidazole-selection. These clone...

journal_title：Molecular and biochemical parasitology

authors： Campos MC,Leon LL,Taylor MC,Kelly JM

更新日期：2014-01-01 00:00:00

abstract：:The complex molecular systems involved in the process of sex-differentiation and fertility in Schistosoma mansoni have not yet been completely described. Using a 4608-element cDNA microarray, we have now determined 90 and 139 genes with significantly (q-value

journal_title：Molecular and biochemical parasitology

authors： DeMarco R,Oliveira KC,Venancio TM,Verjovski-Almeida S

更新日期：2006-12-01 00:00:00