当前位置： SCI文献检索 > MOLECULAR AND BIOCHEMICAL PARASITOLOGY期刊下所有文献 > Amplification and sequencing of genomic DNA fragments encoding cysteine proteases from protozoan parasites.

Amplification and sequencing of genomic DNA fragments encoding cysteine proteases from protozoan parasites.

Abstract：

:Cysteine protease gene fragments from three protozoan parasites Trypanosoma cruzi, Trypanosoma brucei, and Entamoeba histolytica were amplified by the polymerase chain reaction (PCR) from genomic DNA using degenerate oligonucleotide primers. The primers used for the amplification were designed based upon amino acid sequences flanking the active site cysteine and asparagine residues that are conserved in the eukaryotic cysteine proteases analyzed to date. The amplified DNA fragments, representing approximately 70% of the coding regions of the cysteine protease genes, were subcloned and sequenced. Sequence analysis and alignment showed significant sequence similarity to other members of the eukaryotic cysteine protease family (45% identical to chicken cathepsin L) and conservation of the cysteine, histidine, and asparagine residues which form the catalytic triad. These gene fragments provide molecular probes for further analysis of the structure and function of these important metabolic enzymes.

journal_name

Mol Biochem Parasitol

journal_title

Molecular and biochemical parasitology

authors

Eakin AE,Bouvier J,Sakanari JA,Craik CS,McKerrow JH

doi

10.1016/0166-6851(90)90002-4

subject

Has Abstract

pub_date

1990-02-01 00:00:00

pages

1-8

issue

1

eissn

0166-6851

issn

1872-9428

pii

0166-6851(90)90002-4

journal_volume

39

pub_type

杂志文章

相关文献

MOLECULAR AND BIOCHEMICAL PARASITOLOGY文献大全
  • A high level of mixed Trypanosoma brucei infections in tsetse flies detected by three hypervariable minisatellites.

    abstract：:The issue of whether genetic exchange occurs at a significant frequency in natural populations of Trypanosoma brucei is controversial and one of the arguments against a high frequency has been the apparent lack of host infections with mixtures of trypanosome genotypes. Three minisatellite markers (MS42, CRAM, 292) wit...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/s0166-6851(99)00101-2

    authors： MacLeod A,Turner CM,Tait A

    更新日期：1999-08-20 00:00:00

  • Duplication, gene conversion, and genetic diversity in the species-specific acyl-CoA synthetase gene family of Plasmodium falciparum.

    abstract：:While genes encoding antigens and other highly polymorphic proteins are commonly found in subtelomeres, it is unusual to find a small family of housekeeping genes in these regions. We found that in the species Plasmodium falciparum only, a non-subtelomeric acyl-CoA synthetase (ACS) gene has expanded into a family of d...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2006.06.004

    authors： Bethke LL,Zilversmit M,Nielsen K,Daily J,Volkman SK,Ndiaye D,Lozovsky ER,Hartl DL,Wirth DF

    更新日期：2006-11-01 00:00:00

  • A novel rhoptry protein in Toxoplasma gondii bradyzoites and merozoites.

    abstract：:The secretory organelles of Toxoplasma gondii orchestrate invasion of the host cell and establish the parasitophorous vacuole. Although much has been learned about the roles played by these organelles in invasion by the tachyzoite stage, little is known about the contents or functions of these organelles during bradyz...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2005.08.011

    authors： Schwarz JA,Fouts AE,Cummings CA,Ferguson DJ,Boothroyd JC

    更新日期：2005-12-01 00:00:00

  • Molecular detection of Trichostrongylus species through PCR followed by high resolution melt analysis of ITS-2 rDNA sequences.

    abstract：:Polymerase chain reaction followed by high resolution melting (PCR-HRM) analysis is a simple, rapid and accurate method for molecular detection of various nematode species. The objective of the present study was, for the first time, to develop a PCR-HRM assay for the detection of various animal Trichostrongylus spp. A...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2020.111260

    authors： Arbabi M,Hooshyar H,Lotfinia M,Bakhshi MA

    更新日期：2020-03-01 00:00:00

  • Down-regulation of flavin reductase and alcohol dehydrogenase-1 (ADH1) in metronidazole-resistant isolates of Trichomonas vaginalis.

    abstract：:The microaerophilic parasite Trichomonas vaginalis is a causative agent of painful vaginitis or urethritis, termed trichomoniasis, and can also cause preterm delivery or stillbirth. Treatment of trichomoniasis is almost exclusively based on the nitroimidazole drugs metronidazole and tinidazole. Metronidazole resistanc...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2012.03.003

    authors： Leitsch D,Drinić M,Kolarich D,Duchêne M

    更新日期：2012-06-01 00:00:00

  • Presence of contaminating mitochondrial DNA from host reticulocytes in experimental infections of Plasmodium berghei.

    abstract：:Superposition of two unrelated processes, namely terminal reticulocyte differentiation and synchronous plasmodial development, takes place in experimental infections of Plasmodium berghei. The first process is shown to be responsible for the appearance of some discrete restriction bands of host origin when DNA is extr...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(89)90107-2

    authors： Pace T,Ponzi M,Dore E,Scotti R,Mons B

    更新日期：1989-11-01 00:00:00

  • Zinc finger nuclease technology: A stable tool for high efficiency transformation in bloodstream form T. brucei.

    abstract：:In Trypanosoma brucei, the generation of knockout mutants is relatively easy compared to other organisms as transfection methods are well established. These methods have their limitations, however, when it comes to the generation of genome-wide libraries that require a minimum of several hundred thousand transformants...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2017.02.002

    authors： Schumann G,Kangussu-Marcolino MM,Doiron N,Käser S,de Assis Burle-Caldas G,DaRocha WD,Teixeira SM,Roditi I

    更新日期：2017-04-01 00:00:00

  • Characterization of the gene family encoding a host-protective antigen of the tapeworm Taenia ovis.

    abstract：:Genomic structure has been determined for a gene encoding a host-protective antigen of the parasitic platyhelminth Taenia ovis. An incomplete cDNA, known as 45W, encodes the protective antigen. Southern hybridisation experiments using 45W cDNA as a probe, revealed that the 45W gene was a member of a multigene family. ...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(94)00104-u

    authors： Waterkeyn JG,Lightowlers MW,Coppel R,Cowman AF

    更新日期：1995-07-01 00:00:00

  • Characterization of natural antisense transcripts arisen from the locus encoding Toxoplasma gondii ubiquitin-like protease.

    abstract：:Natural antisense transcripts (NATs) are non-protein coding RNAs that could play an important role in regulating the expression of their counterpart protein encoding sense transcript. Although NATs are widespread in most eukaryotic genomes, very little is known about their functions. This study focuses on gaining a be...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2020.111334

    authors： Fahim A,Afrin F,Wen G,Ananvoranich S

    更新日期：2020-11-01 00:00:00

  • Purification of an eight subunit RNA polymerase I complex in Trypanosoma brucei.

    abstract：:Trypanosoma brucei harbors a unique multifunctional RNA polymerase (pol) I which transcribes, in addition to ribosomal RNA genes, the gene units encoding the major cell surface antigens variant surface glycoprotein and procyclin. In consequence, this RNA pol I is recruited to three structurally different types of prom...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2006.02.023

    authors： Nguyen TN,Schimanski B,Zahn A,Klumpp B,Günzl A

    更新日期：2006-09-01 00:00:00

  • Biochemical and functional characterization of CpMuc4, a Cryptosporidium surface antigen that binds to host epithelial cells.

    abstract：:Cryptosporidium spp. are intracellular apicomplexan parasites that cause outbreaks of waterborne diarrheal disease worldwide. Previous studies had identified a Cryptosporidium parvum sporozoite antigen, CpMuc4, that appeared to be involved in attachment and invasion of the parasite into intestinal epithelial cells. Cp...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2014.03.005

    authors： Paluszynski J,Monahan Z,Williams M,Lai O,Morris C,Burns P,O'Connor R

    更新日期：2014-02-01 00:00:00

  • The complexity of the secreted NPA and FAR lipid-binding protein families of Haemonchus contortus revealed by an iterative proteomics-bioinformatics approach.

    abstract：:Two different classes of small nematode specific lipid-binding proteins, the nematode polyprotein allergens/antigens (NPAs) and the fatty acid- and retinol-binding (FAR) proteins, are secreted by helminth parasites. Until now, there was no evidence of the expression or secretion of these two families of proteins in Ha...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2009.07.001

    authors： Kuang L,Colgrave ML,Bagnall NH,Knox MR,Qian M,Wijffels G

    更新日期：2009-11-01 00:00:00

  • Divergent evolutionary constraints on mitochondrial and nuclear genomes of malaria parasites.

    abstract：:Genetic variation among malaria parasites has important consequences with regard to drug resistance, pathogenicity, immunity, transmission, and speciation. In this regard, malaria parasites have been shown to display a high degree of inter- and intra-species genetic divergence. The nuclear genomes of Plasmodium falcip...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/s0166-6851(98)00093-0

    authors： McIntosh MT,Srivastava R,Vaidya AB

    更新日期：1998-09-01 00:00:00

  • Evolution of the microtubular cytoskeleton (flagellar apparatus) in parasitic protists.

    abstract：:The microtubular cytoskeleton of most single-celled eukaryotes radiates from an organizing center called the flagellar apparatus, which is essential for locomotion, feeding and reproduction. The structure of the flagellar apparatus tends to be conserved within diverse clades of eukaryotes, and modifications of this ov...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章,评审

    doi：10.1016/j.molbiopara.2016.02.002

    authors： Yubuki N,Čepička I,Leander BS

    更新日期：2016-01-01 00:00:00

  • Complementation of Saccharomyces cerevisiaepik1ts by a phosphatidylinositol 4-kinase from Plasmodium falciparum.

    abstract：:Phosphoinositides comprise a group of essential phospholipids that control a variety of cellular functions. In the case of the human malaria parasite Plasmodium falciparum, phosphoinositides have been shown to trigger exflagellation and to affect haemoglobin endocytosis and maturation of the parasite's digestive vacuo...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2010.03.020

    authors： Krüger T,Sanchez CP,Lanzer M

    更新日期：2010-08-01 00:00:00

  • The domain on the mouse Duffy protein for Plasmodium yoelii binding and invasion to mouse erythrocytes.

    abstract：:Erythrocyte invasion by malaria parasites is a multi-step process requiring specific molecular interactions between merozoites and erythrocyte surface receptors. Human Duffy blood group protein is the receptor for Plasmodium vivax merozoite invasion to red blood cells. The cognate parasite ligand for Duffy protein is ...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2005.11.005

    authors： Xu L,Niu J,Chaudhuri A

    更新日期：2006-04-01 00:00:00

  • The role of aminopeptidases in haemoglobin degradation in Plasmodium falciparum-infected erythrocytes.

    abstract：:Intra-erythrocytic Plasmodium parasites digest host cell haemoglobin and use the liberated amino acids for protein synthesis. Although several endoproteases (aspartic, cysteine, and metallo-) have been shown to be involved in the initial stages of haemoglobin degradation, little is known about the steps immediately be...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/s0166-6851(01)00327-9

    authors： Gavigan CS,Dalton JP,Bell A

    更新日期：2001-09-28 00:00:00

  • Polymorphism of the alleles of the merozoite surface antigens MSA1 and MSA2 in Plasmodium falciparum wild isolates from Colombia.

    abstract：:The degree of polymorphism and the allelic distribution of 2 major Plasmodium falciparum merozoite surface antigens (MSA1 and MSA2) have been analysed in clinical isolates from Colombia. DNA was prepared directly from patients' blood and used in PCR reactions to amplify block 2 of MSA1 and the central region from MSA2...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(91)90070-m

    authors： Snewin VA,Herrera M,Sanchez G,Scherf A,Langsley G,Herrera S

    更新日期：1991-12-01 00:00:00

  • Linearized free maxicircle DNA in Crithidia fasciculata is a product of topoisomerase II-mediated cleavage.

    abstract：:Linearized free maxicircle DNA, present in detergent lysates of Crithidia fasciculata mitochondria, was thought to be a replication intermediate formed during rolling circle replication of maxicircle DNA. Gel electrophoresis of the linearized free maxicircles indicated that they were slightly larger than the maxicircl...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(95)02550-2

    authors： Carpenter LR,Englund PT

    更新日期：1996-02-01 00:00:00

  • Molecular epidemiology of giardiasis.

    abstract：:Giardia duodenalis is a widespread parasite of mammalian species, including humans. Due to its invariant morphology, investigation on aspects such as host specificity and transmission patterns requires a direct genetic characterization of cysts/trophozoites from host samples. A number of molecular assays have been dev...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章,评审

    doi：10.1016/j.molbiopara.2008.04.006

    authors： Cacciò SM,Ryan U

    更新日期：2008-08-01 00:00:00

  • Identification of Eimeria bovis merozoite cDNAs using differential mRNA display.

    abstract：:Differences in gene expression between Eimeria bovis sporozoites and first-generation merozoites were analyzed using the technique of differential mRNA display. Approx. 5% of the sequences detected in first-generation merozoites appear to be unique relative to sporozoites. Several of the bands corresponding to merozoi...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(94)00052-o

    authors： Abrahamsen MS,Johnson RR,Hathaway M,White MW

    更新日期：1995-05-01 00:00:00

  • Trichomonas vaginalis ribosomal RNA: identification and characterisation of the transcription promoter and terminator sequences.

    abstract：:Trichomonas vaginalis is a parasitic protozoan of both medical and biological relevance. Transcriptional studies in this organism have focused mainly on type II pol promoters, whereas the elements necessary for transcription by polI or polIII have not been investigated. Here, with the aid of a transient transcription ...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2012.05.004

    authors： Franco B,Hernández R,López-Villaseñor I

    更新日期：2012-09-01 00:00:00

  • Expression of substrate-specific transporters encoded by Plasmodium falciparum in Xenopus laevis oocytes.

    abstract：:When the malarial parasite Plasmodium falciparum multiplies in erythrocytes it dramatically increases uptake of essential metabolic precursors (nucleosides, nucleobases and glucose) and export of lactic acid by undefined mechanisms. The first evidence is provided here, by a detailed study in Xenopus laevis oocytes, th...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/s0166-6851(98)00024-3

    authors： Penny JI,Hall ST,Woodrow CJ,Cowan GM,Gero AM,Krishna S

    更新日期：1998-05-15 00:00:00

  • Genetic and antigenic characterization of Babesia bovis merozoite spherical body protein Bb-1.

    abstract：:A Babesia bovis merozoite protein, Bb-1, was localized by immunoelectron microscopy to an apical organelle known as the spherical body. This unique structure appears to be analogous to dense granules of other apicomplexan protozoa. Similar to previously described dense granule proteins of Plasmodium spp., Bb-1 is secr...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(94)00200-7

    authors： Hines SA,Palmer GH,Brown WC,McElwain TF,Suarez CE,Vidotto O,Rice-Ficht AC

    更新日期：1995-02-01 00:00:00

  • Energy metabolism in Cotugnia digonopora and the effect of anthelmintics.

    abstract：:Incorporation of 32Pi into organic phosphate by mitochondria of Cotugnia digonopora was supported maximally by malate. Fumarate and succinate induced lower but significant production of ATP. Pyruvate, alpha-ketoglutarate and oxalacetate proved to be poor substrates and citrate and isocitrate had no effect. A net phosp...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(84)90066-5

    authors： Pampori NA,Singh G,Srivastava VM

    更新日期：1984-04-01 00:00:00

  • The nucleotide sequence of the variable region in Trypanosoma brucei completes the sequence analysis of the maxicircle component of mitochondrial kinetoplast DNA.

    abstract：:The nucleotide sequence of two non-contiguous DNA fragments of 4.0 and 2.2 kb, respectively, of the kinetoplast maxicircle of Trypanosoma brucei brucei EATRO strain 427 has been determined, completing the sequence analysis of the so-called variable region (see also de Vries et al., 1988, Mol. Biochem. Parasitol. 27, 7...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(92)90178-m

    authors： Sloof P,de Haan A,Eier W,van Iersel M,Boel E,van Steeg H,Benne R

    更新日期：1992-12-01 00:00:00

  • The subcellular localisation of trypanosome RRP6 and its association with the exosome.

    abstract：:The exosome, a complex of 3'-exoribonucleases and associated proteins, is involved in the degradation of eukaryotic mRNAs in the cytoplasm, and has RNA processing and quality control functions in the nucleus. In yeast, the nuclear exosome differs from the cytoplasmic one in that it contains an additional non-essential...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2006.10.005

    authors： Haile S,Cristodero M,Clayton C,Estévez AM

    更新日期：2007-01-01 00:00:00

  • Chemical modification of fructose bisphosphate aldolase from Trypanosoma brucei compared to aldolase from rabbit muscle and Staphylococcus aureus.

    abstract：:Chemical modifications of Class I aldolases from Trypanosoma brucei, rabbit muscle and Staphylococcus aureus with carboxypeptidase A, glyceraldehyde 3-phosphate and cysteine-specific reagents revealed the following differences between the three homologous enzymes. Aldolase from S. aureus was not affected by any of the...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(91)90143-t

    authors： Callens M,Opperdoes FR

    更新日期：1991-07-01 00:00:00

  • Developmental and immune regulation of gene expression in Hymenolepis diminuta.

    abstract：:Gene expression and its regulation was studied in the rat tapeworm, Hymenolepis diminuta, during strobilization. RNAs extracted from the different developmental stages of the parasite were translated in vitro in a rabbit reticulocyte lysate system. Two-dimensional patterns of the translational products were compared w...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(87)90014-4

    authors： Siddiqui AA,Karcz SR,Podesta RB

    更新日期：1987-08-01 00:00:00

  • Identification of Toxoplasma TgPH1, a pleckstrin homology domain-containing protein that binds to the phosphoinositide PI(3,5)P2.

    abstract：:The phosphoinositide phosphatidylinositol-3,5-bisphosphate (PI(3,5)P2) plays crucial roles in the maintenance of lysosome/vacuole morphology, membrane trafficking and regulation of endolysosome-localized membrane channel activity. In Toxoplasma gondii, we previously reported that PI(3,5)P2 is essential for parasite su...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2016.03.011

    authors： Daher W,Morlon-Guyot J,Alayi TD,Tomavo S,Wengelnik K,Lebrun M

    更新日期：2016-05-01 00:00:00