Abstract:
:When the malarial parasite Plasmodium falciparum multiplies in erythrocytes it dramatically increases uptake of essential metabolic precursors (nucleosides, nucleobases and glucose) and export of lactic acid by undefined mechanisms. The first evidence is provided here, by a detailed study in Xenopus laevis oocytes, that several specific nutrient transporters are the product of P. falciparum genes. We report the expression of nucleoside, nucleobase, hexose and monocarboxylate transport systems in Xenopus oocytes when injected with mRNA isolated from asexual stages of developing P. falciparum parasites. Their properties are distinct from transport events occurring at the infected erythrocyte membrane or the electrophysiologically identified channel localised to the parasitophorous vacuolar membrane. These novel transporters are substrate-specific and stereoselective, and represent a key regulatory step in the acquisition and export of metabolites by intraerythrocytic P. falciparum.
journal_name
Mol Biochem Parasitoljournal_title
Molecular and biochemical parasitologyauthors
Penny JI,Hall ST,Woodrow CJ,Cowan GM,Gero AM,Krishna Sdoi
10.1016/s0166-6851(98)00024-3subject
Has Abstractpub_date
1998-05-15 00:00:00pages
81-9issue
1eissn
0166-6851issn
1872-9428pii
S0166685198000243journal_volume
93pub_type
杂志文章abstract::Parasitic helminths release molecules into their environment, which are generally referred to as excretory-secretory products or ES. ES derived from a wide range of nematodes, trematodes and cestodes have been studied during the past 30-40 years, their characterization evolving from simple biochemical procedures such ...
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