Abstract：
:Toxocariasis is a neglected disease, and its main etiological agent is the nematode Toxocara canis. Serological diagnosis is performed by an enzyme-linked immunosorbent assay using T. canis excretory and secretory (TES) antigens produced by in vitro cultivation of larvae. Identification of TES proteins can be useful for the development of new diagnostic strategies since few TES components have been described so far. Herein, we report the results obtained by proteomic analysis of TES proteins using a liquid chromatography-tandem mass spectrometry (LC-MS/MS) approach. TES fractions were separated by one-dimensional SDS-PAGE and analyzed by LC-MS/MS. The MS/MS spectra were compared with a database of protein sequences deduced from the genome sequence of T. canis, and a total of 19 proteins were identified. Classification according to the signal peptide prediction using the SignalP server showed that seven of the identified proteins were extracellular, 10 had cytoplasmic or nuclear localization, while the subcellular localization of two proteins was unknown. Analysis of molecular functions by BLAST2GO showed that the majority of the gene ontology (GO) terms associated with the proteins present in the TES sample were associated with binding functions, including but not limited to protein binding (GO:0005515), inorganic ion binding (GO:0043167), and organic cyclic compound binding (GO:0097159). This study provides additional information about the exoproteome of T. canis, which can lead to the development of new strategies for diagnostics or vaccination.
journal_name
Mol Biochem Parasitoljournal_title
Molecular and biochemical parasitologyauthors
Sperotto RL,Kremer FS,Aires Berne ME,Costa de Avila LF,da Silva Pinto L,Monteiro KM,Caumo KS,Ferreira HB,Berne N,Borsuk Sdoi
10.1016/j.molbiopara.2016.09.002subject
Has Abstractpub_date
2017-01-01 00:00:00pages
39-47eissn
0166-6851issn
1872-9428pii
S0166-6851(16)30125-6journal_volume
211pub_type
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journal_title：Molecular and biochemical parasitology
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journal_title：Molecular and biochemical parasitology
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journal_title：Molecular and biochemical parasitology
pub_type： 杂志文章
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journal_title：Molecular and biochemical parasitology
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journal_title：Molecular and biochemical parasitology
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