Expression differential of microsomal and cytosolic glutathione-S-transferases in Fasciola hepatica resistant at triclabendazole.

abstract：:The tegumental membrane of Taenia crassiceps cysticerci contains an ATP-diphosphohydrolase (EC 3.6.1.5) which hydrolyzes purine and pyrimidine nucleoside 5'-di- and 5'-triphosphates at an optimum pH of 8.5. It is Mg(2+)-dependent and insensitive to classical ATPase and phosphatase inhibitors. In solubilized tegumental...

journal_title：Molecular and biochemical parasitology

authors： Guevara-Flores A,Olvera-Sánchez S,Gómez-Concha C,Juárez O,Esparza-Perusquía M,Pardo JP,Mendoza-Hernández G,Martínez F,Flores-Herrera O

更新日期：2008-12-01 00:00:00

abstract：:Kinetoplast DNA (kDNA) networks of the insect trypanosomatid Crithidia fasciculata strain CF-C1 contain a nearly homogeneous population of kDNA minicircles as judged by restriction enzyme cleavage analysis. We have determined the entire nucleotide sequence of the major class of minicircles by analyzing M13 phage clone...

journal_title：Molecular and biochemical parasitology

authors： Sugisaki H,Ray DS

更新日期：1987-04-01 00:00:00

abstract：:We have compared a total of 44 recognition sites for 12 restriction endonucleases on the 20 kilobase pair maxi-circle of kinetoplast DNA from nine Trypanosoma brucei stocks, four which are known to be infective to man (tow 'gambiense' and two 'rhodesiense' variants). In addition to five polymorphic sites, these DNAs d...

journal_title：Molecular and biochemical parasitology

authors： Borst P,Fase-Fowler F,Gibson WC

更新日期：1981-06-01 00:00:00

abstract：:Kinetoplast DNA (kDNA), the mitochondrial DNA of trypanosomes, is a network of thousands of topologically interlocked DNA minicircles and about 50 maxicircles. In this study, we have analysed the kDNA molecules of 6 strains of Trypanosoma evansi from different geographical areas. 2 strains were found to be dyskinetopl...

journal_title：Molecular and biochemical parasitology

authors： Ou YC,Giroud C,Baltz T

更新日期：1991-05-01 00:00:00

abstract：:The apicoplast is a highly specialized organelle that mediates required functions in the growth and replication of apicomplexan parasites. Despite structural conservation of the apicoplast among different parasite genera and species, there are also critical differences in the metabolic requirements of different parasi...

journal_title：Molecular and biochemical parasitology

authors： Caballero MC,Pedroni MJ,Palmer GH,Suarez CE,Davitt C,Lau AO

更新日期：2012-02-01 00:00:00

abstract：:A membrane-bound sialidase (EC 3.2.1.18) was found in procyclic trypomastigotes of Trypanosoma brucei. The mammalian stage bloodstream form, however, displayed no sialidase activity. This sialidase is an integral surface protein, linked to the membrane via a glycosylphosphatidylinositol anchor. After osmotic lysis and...

journal_title：Molecular and biochemical parasitology

authors： Engstler M,Reuter G,Schauer R

更新日期：1992-08-01 00:00:00

abstract：:Tubulin-binding cofactor C stimulates GTPase activity and contributes to the release of the heterodimeric α/β-tubulin from a super-complex of tubulin monomers and two ancillary cofactors. We have determined the 2.2 Å resolution crystal structure of the C-terminal domain of tubulin-binding cofactor C from Leishmania ma...

journal_title：Molecular and biochemical parasitology

authors： Barrack KL,Fyfe PK,Finney AJ,Hunter WN

更新日期：2015-05-01 00:00:00

abstract：:The karyotype and chromosomes of the human malaria parasite Plasmodium falciparum have been well characterized in recent years. Here we present karyotype maps of the three other human malaria species, P. vivax, P. malariae and P. ovale. Chromosomes of these species were found to be of significantly higher molecular we...

journal_title：Molecular and biochemical parasitology

authors： Carlton JM,Galinski MR,Barnwell JW,Dame JB

更新日期：1999-06-25 00:00:00

abstract：:The degree of polymorphism and the allelic distribution of 2 major Plasmodium falciparum merozoite surface antigens (MSA1 and MSA2) have been analysed in clinical isolates from Colombia. DNA was prepared directly from patients' blood and used in PCR reactions to amplify block 2 of MSA1 and the central region from MSA2...

journal_title：Molecular and biochemical parasitology

authors： Snewin VA,Herrera M,Sanchez G,Scherf A,Langsley G,Herrera S

更新日期：1991-12-01 00:00:00

abstract：:Sj23, the 23-kDa target antigen in Schistosoma japonicum adult worms of the hybridoma monoclonal antibody (mAb) I-134, has been identified and cloned from cDNA libraries, mAb I-134 has been successfully used in immunodiagnostic assays to detect S. japonicum infection in Philippine patients. Sequence analysis has shown...

journal_title：Molecular and biochemical parasitology

authors： Davern KM,Wright MD,Herrmann VR,Mitchell GF

更新日期：1991-09-01 00:00:00

abstract：:An expression vector was constructed to express foreign genes in Trypanosoma congolense. The foreign gene and a neomycin phosphotransferase (NPT) gene are flanked by glutamate and alanine rich protein (GARP) gene processing signals and their expression is driven by a ribosomal RNA gene promoter. The plasmid is not mai...

journal_title：Molecular and biochemical parasitology

authors： Downey N,Donelson JE

更新日期：1999-10-25 00:00:00

abstract：:Sequestration of Plasmodium falciparum infected erythrocytes in the cerebral circulation is strongly implicated in the pathogenesis of cerebral malaria. From previous studies it was postulated that genes essential for cytoadherence were located on the right arm of chromosome 9 as P. falciparum isolates with a deletion...

journal_title：Molecular and biochemical parasitology

authors： Chaiyaroj SC,Coppel RL,Magowan C,Brown GV

更新日期：1994-09-01 00:00:00

abstract：:The specificity of a range of 125I labelling techniques (Chloramine T, Iodogen, Bolton and Hunter reagent, lactoperoxidase and iodosulfanilic acid) to the surface of the filarial nematode Brugia pahangi was evaluated by autoradiography of sections of labelled worms and of dried SDS-polyacrylamide gels following electr...

journal_title：Molecular and biochemical parasitology

authors： Marshall E,Howells RE

更新日期：1985-06-01 00:00:00

abstract：:A family of Schistosoma mansoni proteins (18-22 kDa, pI 5.3-5.8) are biosynthesized in juvenile worms and immunoprecipitated by antibodies uniquely present in protective Fischer rat antiserum. A cDNA clone, lambda gt11-40, expressing epitopes common to this protein family was used to obtain a genomic DNA clone, by hyb...

journal_title：Molecular and biochemical parasitology

authors： el-Sherbeini M,Ramadan N,Bostian KA,Knopf PM

更新日期：1991-11-01 00:00:00

abstract：:A Mo7-derived Babesia bovis line stably transfected with the gfp-bsd gene was inoculated into two four to five months old calves, while two additional calves were inoculated with Mo7 parasites. Similar mild clinical signs were detected in all calves. B. bovis rap-1 was identified in the bloodstream by PCR four days po...

journal_title：Molecular and biochemical parasitology

authors： Suarez CE,Laughery JM,Schneider DA,Sondgeroth KS,McElwain TF

更新日期：2012-09-01 00:00:00

abstract：:The nucleotide sequence of two non-contiguous DNA fragments of 4.0 and 2.2 kb, respectively, of the kinetoplast maxicircle of Trypanosoma brucei brucei EATRO strain 427 has been determined, completing the sequence analysis of the so-called variable region (see also de Vries et al., 1988, Mol. Biochem. Parasitol. 27, 7...

journal_title：Molecular and biochemical parasitology

authors： Sloof P,de Haan A,Eier W,van Iersel M,Boel E,van Steeg H,Benne R

更新日期：1992-12-01 00:00:00

abstract：:Incorporation of 32Pi into organic phosphate by mitochondria of Cotugnia digonopora was supported maximally by malate. Fumarate and succinate induced lower but significant production of ATP. Pyruvate, alpha-ketoglutarate and oxalacetate proved to be poor substrates and citrate and isocitrate had no effect. A net phosp...

journal_title：Molecular and biochemical parasitology

authors： Pampori NA,Singh G,Srivastava VM

更新日期：1984-04-01 00:00:00

abstract：:Soon after infecting a mammalian host, cercariae of Schistosoma mansoni rapidly undergo a series of morphologic and biochemical adaptations associated with transformation to their next developmental stage, the schistosomulum. Few of these changes are associated with alterations in gene expression except for an apparen...

journal_title：Molecular and biochemical parasitology

authors： Blanton RE,Licate LS

更新日期：1992-04-01 00:00:00

abstract：:Human hookworms (Ancylostoma duodenale, Necator americanus) are a major cause of malnutrition and anemia, particularly in children, and high worm burdens can lead to stunted growth and mental retardation. Mass drug administration (MDA) with benzimidazole (BZ) anthelmintics has the potential to greatly reduce morbidity...

journal_title：Molecular and biochemical parasitology

authors： Schwenkenbecher JM,Albonico M,Bickle Q,Kaplan RM

更新日期：2007-12-01 00:00:00

abstract：:Four Trypanosoma cruzi genomic DNA fragments carrying different TcP2 beta genes have been isolated and sequenced. Three of them had a single TcP2 beta gene, while the 3.8-kb-long DNA segment encoding the TcP2 beta-H1.8 locus showed two TcP2 beta genes arranged in tandem. These genes were physically connected by a 428-...

journal_title：Molecular and biochemical parasitology

authors： Vazquez MP,Schijman AG,Levin MJ

更新日期：1994-04-01 00:00:00

abstract：:In Ascaris muscle mitochondria the major respiratory chain-linked phosphorylation activity is accomplished by a NADH-linked reduction of fumarate to succinate. Oxygen can also be employed as a terminal electron acceptor via a cyanide- and salicyl-hydroxamate-resistant terminal oxidase. As in fumarate-dependent electro...

journal_title：Molecular and biochemical parasitology

authors： Köhler P,Bachmann R

更新日期：1980-04-01 00:00:00

abstract：:Hookworm disease is a debilitating worm infection that affects hundreds of millions of people. Despite the existence of anthelmintic drugs, reports have testified of a decrease in efficacy of these drugs. Therefore, it is imperative to find new drugs and drug targets for hookworm disease treatment. In this study we id...

journal_title：Molecular and biochemical parasitology

authors： Rigouin C,Vermeire JJ,Nylin E,Williams DL

更新日期：2013-09-01 00:00:00

abstract：:Blood-stage malaria vaccine candidates include surface proteins of the merozoite. Antibodies to these proteins may either block essential steps during invasion or render the merozoite susceptible to phagocytosis or complement-mediated degradation. Structural information on merozoite surface proteins complexed to antib...

journal_title：Molecular and biochemical parasitology

authors： Dekker C,Uthaipibull C,Calder LJ,Lock M,Grainger M,Morgan WD,Dodson GG,Holder AA

更新日期：2004-09-01 00:00:00

abstract：:The smallest chromosome (230 kb) of the HU3 stock of Leishmania donovani was purified from an orthogonal field alternation (OFAGE) gel, digested with PstI and cloned into the plasmid pUC13. When used to probe Southern blots of OFAGE gels, the cloned sequences recognised one or more large chromosomes in all L. donovani...

journal_title：Molecular and biochemical parasitology

authors： Bishop RP

更新日期：1990-01-01 00:00:00

abstract：:The aspartic protease cathepsin D (Clan AA, Family A1) is expressed in the schistosome gut where it plays an apical role in the digestion of hemoglobin released from ingested erythrocytes. In this report, RNA interference approaches were employed to investigate the effects of knockdown of schistosome cathepsin D. Cult...

journal_title：Molecular and biochemical parasitology

authors： Morales ME,Rinaldi G,Gobert GN,Kines KJ,Tort JF,Brindley PJ

更新日期：2008-02-01 00:00:00

abstract：:The synchronization of intraerythrocytic maturation of Plasmodium parasites is an important factor in the malaria infection process. Synchronization is mediated by inositol phosphate (InsP(x))-induced Ca(2+)-release from internal stores. To further investigate the InsP(x) metabolism in these parasites a Plasmodium pro...

journal_title：Molecular and biochemical parasitology

authors： Stritzke C,Nalaskowski MM,Fanick W,Lin H,Mayr GW

更新日期：2012-12-01 00:00:00

abstract：:Chloroquine is a critically important antimalarial drug and a well known intercalator into DNA. We now show that chloroquine binds more avidly to poly(dG-dC).poly(dG-dC) than to other synthetic polynucleotides and that this binding inhibits both salt- and cobalt-induced transitions to Z-DNA. These data are consistent ...

journal_title：Molecular and biochemical parasitology

authors： Kwakye-Berko F,Meshnick S

更新日期：1990-03-01 00:00:00

abstract：:Schistosoma mansoni eggs, consisting of an ovum surrounded by nutritive vitelline cells packaged in a tanned protein shell, are produced by paired worms residing in the mesenteric veins of the human host. The vitelline cells are degraded as the larval miracidium matures, the fully developed egg either crossing the gut...

journal_title：Molecular and biochemical parasitology

authors： Mathieson W,Castro-Borges W,Wilson RA

更新日期：2011-02-01 00:00:00

abstract：:The extent of structural conservation of the Plasmodium falciparum sporozoite surface protein gene, STARP, recently characterized in the T9/96 clone, has been analyzed using the polymerase chain reaction. Results from Ivory Coast and Thai clones, field isolates originating from Brazil and Kenya and laboratory-maintain...

journal_title：Molecular and biochemical parasitology

authors： Fidock DA,Sallenave-Sales S,Sherwood JA,Gachihi GS,Ferreira-da-Cruz MF,Thomas AW,Druilhe P

更新日期：1994-10-01 00:00:00

abstract：:The merozoite surface protein-1 of the human malaria parasite Plasmodium falciparum undergoes an extracellular proteolytic cleavage (secondary processing) intrinsic to successful erythrocyte invasion. In the T9/96 clone of P. falciparum the protease responsible has been characterised as a membrane-associated, calcium-...

journal_title：Molecular and biochemical parasitology

authors： Blackman MJ,Chappel JA,Shai S,Holder AA

更新日期：1993-11-01 00:00:00