Expression differential of microsomal and cytosolic glutathione-S-transferases in Fasciola hepatica resistant at triclabendazole.


:In the present work, we evaluate in vitro the cytosolic and microsomal activity of glutathione-S-transferases in adults of Fasciola hepatica susceptible (Cullompton Strain) and resistant (Sligo Strain) to triclabendazole. The triclabendazole resistant (Sligo) fluke expressed significant major metabolic activity of glutathione-S-transferases compared to that measured in the cytosolic and microsomal fractions obtained from susceptible flukes (Cullompton Strain). The results associated with previous data where the Sligo Strain overexpress Flavin Monooxigenase confirm a multienzymatic response involving more than one metabolic pathway.


Mol Biochem Parasitol


Scarcella S,Lamenza P,Virkel G,Solana H




Has Abstract


2012-01-01 00:00:00














  • Three genes and two isozymes: gene conversion and the compartmentalization and expression of the phosphoglycerate kinases of Trypanosoma (Nannomonas) congolense.

    abstract::The glycosome, a microbody organelle found only in kinetoplastid protozoa, compartmentalizes the first six enzymes of glycolysis. In order to better understand the regulation and targeting of glycolytic enzymes in trypanosomes, we have cloned and analyzed the three genes of the phosphoglycerate kinase (PGK) complex of...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Parker HL,Hill T,Alexander K,Murphy NB,Fish WR,Parsons M

    更新日期:1995-02-01 00:00:00

  • E. dispar strain: analysis of polymorphism as a tool for study of geographic distribution.

    abstract::The intra-species polymorphism of E. histolytica and E. dispar species in endemic area is an important tool for geographic distribution and spread mechanism studies. Since E. dispar and E. histolytica shears ecological niche, cell cycle, and transmission mechanism for human host, we studied the intra-specie variation ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ramos F,García G,Valadez A,Morán P,González E,Gómez A,Melendro EI,Valenzuela O,Ximénez C

    更新日期:2005-06-01 00:00:00

  • Energy metabolism in Cotugnia digonopora and the effect of anthelmintics.

    abstract::Incorporation of 32Pi into organic phosphate by mitochondria of Cotugnia digonopora was supported maximally by malate. Fumarate and succinate induced lower but significant production of ATP. Pyruvate, alpha-ketoglutarate and oxalacetate proved to be poor substrates and citrate and isocitrate had no effect. A net phosp...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Pampori NA,Singh G,Srivastava VM

    更新日期:1984-04-01 00:00:00

  • Genomic organisation of nuclear tRNAGly and tRNALeu genes in Trypanosoma brucei.

    abstract::We have isolated a 0.3-kb HaeIII restriction fragment from Trypanosoma brucei which contains two tRNA genes. Secondary structure models predict that the two genes identified encode tRNA molecules which specify glycine (anticodon UCC) and leucine (anticodon CAG). The two genes are separated by 86 nucleotides, transcrib...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Campbell DA,Suyama Y,Simpson L

    更新日期:1989-12-01 00:00:00

  • Complementation of Saccharomyces cerevisiaepik1ts by a phosphatidylinositol 4-kinase from Plasmodium falciparum.

    abstract::Phosphoinositides comprise a group of essential phospholipids that control a variety of cellular functions. In the case of the human malaria parasite Plasmodium falciparum, phosphoinositides have been shown to trigger exflagellation and to affect haemoglobin endocytosis and maturation of the parasite's digestive vacuo...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Krüger T,Sanchez CP,Lanzer M

    更新日期:2010-08-01 00:00:00

  • Characterization of a polymorphic Theileria annulata surface protein (TaSP) closely related to PIM of Theileria parva: implications for use in diagnostic tests and subunit vaccines.

    abstract::Theileria annulata is a tick-transmitted protozoan that causes tropical theileriosis, an often fatal leukoproliferative disorder of cattle. To characterize and identify parasite proteins suitable as diagnostic antigens and/or vaccine candidates, a cDNA clone encoding a macroschizont stage protein was isolated and char...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Schnittger L,Katzer F,Biermann R,Shayan P,Boguslawski K,McKellar S,Beyer D,Shiels BR,Ahmed JS

    更新日期:2002-04-09 00:00:00

  • Some kinetic properties of pyruvate kinase from Trypanosoma brucei: influence of pH and fructose-1,6-diphosphate.

    abstract::The influence of pH on the activity of purified pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC from Trypanosoma brucei has been studied. The Km for the coenzyme ADP is pH-dependent and shows the involvement of a dissociable group on the free enzyme with a pKa of 6.5-6.7. The cooperative interaction...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Flynn IW,Bowman IB

    更新日期:1981-11-01 00:00:00

  • Molecular and functional characterisation of the heat shock protein 10 of Strongyloides ratti.

    abstract::Strongyloides stercoralis and S. ratti are intestinal parasitic nematodes infecting rats and humans, respectively. Both present extraordinary life cycles comprising a free-living generation in addition to parasitic stages. In search of molecules possibly involved in parasite-host interaction, we performed mass spectro...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Tazir Y,Steisslinger V,Soblik H,Younis AE,Beckmann S,Grevelding CG,Steen H,Brattig NW,Erttmann KD

    更新日期:2009-12-01 00:00:00

  • Structural and functional identification of GP57/51 antigen of Trypanosoma cruzi as a cysteine proteinase.

    abstract::Purified GP57/51, a Trypanosoma cruzi glycoprotein earlier identified as a major antigen in infected humans, was subjected to N-terminal sequence analysis. Alignment of the first 30 amino acids revealed that its N-terminal region is virtually identical to that reported for a cysteine-proteinase isolated from the Tulah...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Murta AC,Persechini PM,Padron Tde S,de Souza W,Guimarães JA,Scharfstein J

    更新日期:1990-11-01 00:00:00

  • A developmentally regulated cysteine protease gene family in Haemonchus contortus.

    abstract::The nucleotide sequence of a gene encoding a 35-kDa thiol protease of the parasitic nematode Haemonchus contortus has been determined. The gene, designated AC-2, shares 97% nucleotide sequence identity and 98% amino acid identity with previously characterized AC-1 cDNAs encoding the thiol protease. The AC-2 gene spans...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Pratt D,Cox GN,Milhausen MJ,Boisvenue RJ

    更新日期:1990-12-01 00:00:00

  • The kinetic properties and sensitivities to inhibitors of lactate dehydrogenases (LDH1 and LDH2) from Toxoplasma gondii: comparisons with pLDH from Plasmodium falciparum.

    abstract::Toxoplasma gondii differentially expresses two forms of lactate dehydrogenase in tachyzoites and bradyzoites, respectively, designated LDH1 and LDH2. Previously it was demonstrated that LDH1 and LDH2 share a unique structural feature with LDH from the malarial parasite Plasmodium falciparum (pLDH), namely, the additio...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Dando C,Schroeder ER,Hunsaker LA,Deck LM,Royer RE,Zhou X,Parmley SF,Vander Jagt DL

    更新日期:2001-11-01 00:00:00

  • Mimicry of elastin repetitive motifs by Theileria annulata sporozoite surface antigen.

    abstract::Theileria annulata is an important pathogen of cattle in the tropics. The gene sequence of a sporozoite surface antigen (SPAG-1) is reported. Data is also presented demonstrating that SPAG-1 is synthesised as a large precursor. This antigen, which is a candidate for inclusion in a subunit vaccine, shows a remarkable d...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hall R,Hunt PD,Carrington M,Simmons D,Williamson S,Mecham RP,Tait A

    更新日期:1992-07-01 00:00:00

  • L-myo-Inositol 1-phosphate synthase from Entamoeba histolytica.

    abstract::L-myo-Inositol 1-phosphate synthase (I-1-P synthase) catalyses the primary reaction for the synthesis of inositol in a variety of prokaryotes, eukaryotes and in the chloroplasts of algae and higher plants. Inositol is a precursor of essential macromolecules like membrane phospholipids, GPI anchor proteins and lipophos...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Lohia A,Hait NC,Majumder AL

    更新日期:1999-01-05 00:00:00

  • 5'-p-Fluorosulfonyl benzoyl adenosine inhibits an ecto-ATP-diphosphohydrolase in the tegument surface of Taenia crassiceps cysticerci.

    abstract::The tegumental membrane of Taenia crassiceps cysticerci contains an ATP-diphosphohydrolase (EC which hydrolyzes purine and pyrimidine nucleoside 5'-di- and 5'-triphosphates at an optimum pH of 8.5. It is Mg(2+)-dependent and insensitive to classical ATPase and phosphatase inhibitors. In solubilized tegumental...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Guevara-Flores A,Olvera-Sánchez S,Gómez-Concha C,Juárez O,Esparza-Perusquía M,Pardo JP,Mendoza-Hernández G,Martínez F,Flores-Herrera O

    更新日期:2008-12-01 00:00:00

  • Molecules on the surface of the Plasmodium falciparum infected erythrocyte and their role in malaria pathogenesis and immune evasion.

    abstract::The surface of the erythrocyte undergoes a number of modifications during infection by Plasmodium falciparum. These modifications are critical for pathogenesis of severe disease and the acquisition of host immunity through their role in interactions between the host and the parasite and in antigenic variation. Our kno...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Craig A,Scherf A

    更新日期:2001-07-01 00:00:00

  • Two-dimensional electrophoretic separation and fluorographic analysis of the gene products synthesized by Hymenolepis diminuta with particular reference to the parasite-specific polypeptides in the brush border membrane.

    abstract::This study is the first demonstration of individual gene products synthesized by Hymenolepis diminuta. To identify proteins synthesized specifically by H. diminuta, we radiolabeled adult worms with [14C]leucine in vitro. Subsequent one- and two-dimensional gel electrophoretic separation and staining of the protein con...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Atkinson BG,Podesta RB

    更新日期:1982-07-01 00:00:00

  • Transcriptomic analysis of reduced sensitivity to praziquantel in Schistosoma mansoni.

    abstract::Schistosomiasis is an intravascular parasitic infection estimated to affect over 206 million people, the majority of whom live in Africa where the trematode worms Schistosoma mansoni and Schistosoma haematobium are the major causative agents. While a number of drugs have been used to treat schistosomiasis, praziquante...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Sanchez MC,Cupit PM,Bu L,Cunningham C

    更新日期:2019-03-01 00:00:00

  • RNA interference in Pratylenchus coffeae: knock down of Pc-pat-10 and Pc-unc-87 impedes migration.

    abstract::Many of the currently available nematicides used in nematode control are hazardous to the user, environment and beneficial non-target organisms. Therefore the need to develop alternative methods for nematode control such as the development of nematode-resistant crops through RNA-mediated interference (RNAi) holds grea...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Joseph S,Gheysen G,Subramaniam K

    更新日期:2012-11-01 00:00:00

  • Cloning and sequence analysis of the Schistosoma mansoni membrane glycoprotein antigen gene GP22.

    abstract::A family of Schistosoma mansoni proteins (18-22 kDa, pI 5.3-5.8) are biosynthesized in juvenile worms and immunoprecipitated by antibodies uniquely present in protective Fischer rat antiserum. A cDNA clone, lambda gt11-40, expressing epitopes common to this protein family was used to obtain a genomic DNA clone, by hyb...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: el-Sherbeini M,Ramadan N,Bostian KA,Knopf PM

    更新日期:1991-11-01 00:00:00

  • Nucleotide binding properties of a P-glycoprotein homologue from Plasmodium falciparum.

    abstract::The Plasmodium falciparum P-glycoprotein homologue 1 (PGH1) is structurally similar to several members of the ATP-binding cassette (ABC) superfamily of membrane transporters. We have examined whether the nucleotide binding domains predicted from the deduced amino sequence are functional by photoaffinity labeling of pu...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Karcz SR,Galatis D,Cowman AF

    更新日期:1993-04-01 00:00:00

  • Characterisation of a simple, highly repetitive DNA sequence from the parasite Leishmania donovani.

    abstract::Repetitive DNA sequences of the Leishmania donovani genome have been identified by screening a recombinant DNA library made by cloning sheared genomic DNA into the vector pAT153. Bacterial clones containing a highly repetitive DNA sequence have been isolated. DNA sequencing has shown that this sequence is composed of ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ellis J,Crampton J

    更新日期:1988-05-01 00:00:00

  • Purification and characterization of gamma-glutamylcysteine synthetase from Ascaris suum.

    abstract::We have purified and characterized the Ascaris suum gamma-glutamylcysteine synthetase, the rate-limiting step in the glutathione biosynthesis. The purified enzyme exhibited a specific activity of 18 U (mg protein)-1. Estimation of the molecular mass of the native enzyme by FPLC on Superdex S-200 revealed the presence ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hussein AS,Walter RD

    更新日期:1995-06-01 00:00:00

  • Characterization of novel fucosyl- and tyvelosyl-containing glycoconjugates from Trichinella spiralis muscle stage larvae.

    abstract::The monosaccharide composition of an affinity-purified family of antigenically-related Trichinella spiralis larval glycoproteins was determined by gas chromatography/mass spectrometry. This group of 6 major glycoproteins, designated TSL-1, originates in the muscle stage (L1) larval stichosome. They are present on the ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Wisnewski N,McNeil M,Grieve RB,Wassom DL

    更新日期:1993-09-01 00:00:00

  • Role of porcine serum haptoglobin in the host-parasite relationship of Taenia solium cysticercosis.

    abstract::Human and porcine cysticercosis is a parasitic disease caused by the larval stage (cysts) of the tapeworm Taenia solium. Cysts may live in several host tissues such as skeletal muscle or brain. We have previously described the presence of host haptoglobin (Hp) and hemoglobin (Hb) in different protein extracts of the T...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Navarrete-Perea J,Toledano-Magaña Y,De la Torre P,Sciutto E,Bobes RJ,Soberón X,Laclette JP

    更新日期:2016-06-01 00:00:00

  • Cathepsin L proteinase secreted by Fasciola hepatica in vitro prevents antibody-mediated eosinophil attachment to newly excysted juveniles.

    abstract::Cathepsin L-like activity was demonstrated in the excretory/secretory (E/S) products of Fasciola hepatica newly excysted juveniles (NEJ), 3-week-old, 5-week-old and mature flukes using the fluorogenic substituted 7-amino-4-methylcoumarin substrates Z-phe-arg-AMC, Z-arg-arg-AMC and Z-arg-AMC. Gelatin-substrate polyacry...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Carmona C,Dowd AJ,Smith AM,Dalton JP

    更新日期:1993-11-01 00:00:00

  • Identification by extrachromosomal amplification and overexpression of a zeta-crystallin/NADPH-oxidoreductase homologue constitutively expressed in Leishmania spp.

    abstract::A gene which overexpresses a 36-kDa protein (p36) in tunicamycin-resistant Leishmania was mapped by transfection and overexpression to the upstream region of the drug maker in the extrachromosomal amplicon. Complete sequencing of this region revealed a single open reading frame of about 1 kb. Authenticity of the clone...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Liu X,Chang KP

    更新日期:1994-08-01 00:00:00

  • Population genetic analysis and sub-structuring in Babesia bovis.

    abstract::The tick-borne protozoan parasite, Babesia bovis is one of the causes of bovine babesiosis, an economically important disease of cattle in tropical and sub-tropical countries. Using the recently published genome sequence of the parasite, we developed a panel of eight mini- and micro-satellite markers and used these to...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Simuunza M,Bilgic H,Karagenc T,Syakalima M,Shiels B,Tait A,Weir W

    更新日期:2011-06-01 00:00:00

  • A population of tRNA-derived small RNAs is actively produced in Trypanosoma cruzi and recruited to specific cytoplasmic granules.

    abstract::Over the last years an expanding family of small RNAs (i.e. microRNAs, siRNAs and piRNAs) was recognized as key players in diverse forms of gene silencing and chromatin organization. Effectors functions of these small RNAs are achieved through ribonucleoprotein (RNP) complexes containing at their center an Argonaute/P...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Garcia-Silva MR,Frugier M,Tosar JP,Correa-Dominguez A,Ronalte-Alves L,Parodi-Talice A,Rovira C,Robello C,Goldenberg S,Cayota A

    更新日期:2010-06-01 00:00:00

  • The mature-parasite-infected erythrocyte surface antigen (MESA) of Plasmodium falciparum associates with the erythrocyte membrane skeletal protein, band 4.1.

    abstract::Several proteins synthesized by mature asexual stages of Plasmodium falciparum interact with the erythrocyte membrane skeleton. One of these is the mature-parasite-infected erythrocyte surface antigen (MESA; also called PfEMP2), a phosphoprotein of 250-300 kDa, which is found on the internal face of the erythrocyte me...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Lustigman S,Anders RF,Brown GV,Coppel RL

    更新日期:1990-01-15 00:00:00

  • The subcellular localisation of trypanosome RRP6 and its association with the exosome.

    abstract::The exosome, a complex of 3'-exoribonucleases and associated proteins, is involved in the degradation of eukaryotic mRNAs in the cytoplasm, and has RNA processing and quality control functions in the nucleus. In yeast, the nuclear exosome differs from the cytoplasmic one in that it contains an additional non-essential...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Haile S,Cristodero M,Clayton C,Estévez AM

    更新日期:2007-01-01 00:00:00