Expression differential of microsomal and cytosolic glutathione-S-transferases in Fasciola hepatica resistant at triclabendazole.

abstract：:Variation of Babesia bovis merozoite surface antigens occurs among geographic strains of the parasite. In this and a concurrent report, we investigate this variation at the gene and protein level. Using a monoclonal antibody (mAb 23/70.174), B. bovis gene sequences were identified that encoded a surface epitope of a 4...

journal_title：Molecular and biochemical parasitology

authors： Jasmer DP,Reduker DW,Hines SA,Perryman LE,McGuire TC

更新日期：1992-10-01 00:00:00

abstract：:Monoclonal antibodies designated 8G10/48 and 9E3/48 raised against mature asexual blood stages of Plasmodium falciparum inhibit parasite growth in vitro. Both antibodies bind to an epitope which includes the linear sequence Ser Thr Asn Ser and which is present in a cDNA clone from a P. falciparum expression library. T...

journal_title：Molecular and biochemical parasitology

authors： Epping RJ,Goldstone SD,Ingram LT,Upcroft JA,Ramasamy R,Cooper JA,Bushell GR,Geysen HM

更新日期：1988-02-01 00:00:00

abstract：:Genomic structure has been determined for a gene encoding a host-protective antigen of the parasitic platyhelminth Taenia ovis. An incomplete cDNA, known as 45W, encodes the protective antigen. Southern hybridisation experiments using 45W cDNA as a probe, revealed that the 45W gene was a member of a multigene family. ...

journal_title：Molecular and biochemical parasitology

authors： Waterkeyn JG,Lightowlers MW,Coppel R,Cowman AF

更新日期：1995-07-01 00:00:00

abstract：:The subcellular location of the major malarial glycoprotein in erythrocytes infected with schizonts of Plasmodium falciparum has been studied by two methods. In the first, glycoproteins were labelled with [3H]glucosamine or [3H]isoleucine during in vitro culture. Trypsin treatment of intact infected erythrocytes cause...

journal_title：Molecular and biochemical parasitology

authors： Howard RJ,Lyon JA,Diggs CL,Haynes JD,Leech JH,Barnwell JW,Aley SB,Aikawa M,Miller LH

更新日期：1984-04-01 00:00:00

abstract：:A convenient method was employed for the efficient extraction of total RNA from Plasmodium falciparum. By depleting the total RNA of tRNA, it was shown that P. falciparum or P. lophurae tRNAs markedly stimulate the translation of P. falciparum mRNA in the rabbit reticulocyte cell-free system. Analysis of the cell-free...

journal_title：Molecular and biochemical parasitology

authors： Wallach M

更新日期：1982-12-01 00:00:00

abstract：:The electrophoretic patterns of hexokinase and phosphoglucomutase have been widely used to distinguish Entamoeba histolytica from Entamoeba dispar isolates. Although E. histolytica and E. dispar, previously called pathogenic and nonpathogenic Entamoeba histolytica, differ clearly in sequences of many homologous genes,...

journal_title：Molecular and biochemical parasitology

authors： Ortner S,Clark CG,Binder M,Scheiner O,Wiedermann G,Duchêne M

更新日期：1997-05-01 00:00:00

abstract：:The obligate intracellular protozoan parasite Toxoplasma gondii has a single tubular mitochondrion. During infection, it recruits the host cell's mitochondria abutting to the intracellular vacuole, that contains the parasites. The respective contribution of host and parasitic mitochondria in the intracellular growth o...

journal_title：Molecular and biochemical parasitology

authors： Toursel C,Dzierszinski F,Bernigaud A,Mortuaire M,Tomavo S

更新日期：2000-12-01 00:00:00

abstract：:Trypanosoma brucei is a unicellular parasite that is transmitted from one mammalian host to the next by tsetse flies. The expression of many trypanosome genes is regulated during the life cycle but there is no evidence for developmental control of transcription by RNA polymerase II. T. brucei expresses at least two he...

journal_title：Molecular and biochemical parasitology

authors： Hotz HR,Lorenz P,Fischer R,Krieger S,Clayton C

更新日期：1995-12-01 00:00:00

abstract：:Glutathione S-transferases (GSTs) constitute a major detoxification mechanism in helminth organisms and are regarded vaccine candidates against helminth infections. Onchocerca volvulus glutathione-binding proteins were purified from the aqueous soluble fraction of homogenised adult females by affinity chromatography o...

journal_title：Molecular and biochemical parasitology

authors： Salinas G,Braun G,Taylor DW

更新日期：1994-07-01 00:00:00

abstract：:Species of Centrorhynchus (Polymorphida: Centrorhynchidae) commonly parasitize various falconiform and strigiform birds worldwide. In the present study, the complete mitochondrial (mt) genome sequences of Centrorhynchus clitorideus was sequenced and annotated for the first time based on specimens collected from the li...

journal_title：Molecular and biochemical parasitology

authors： Muhammad N,Suleman,Khan MS,Li L,Zhao Q,Ullah H,Zhu XQ,Ma J

更新日期：2020-05-01 00:00:00

abstract：:Cryptosporidium spp. are waterborne apicomplexan parasites responsible for outbreaks of diarrheal disease worldwide. Antigens involved in zoite invasion into host cells have been the focus of many investigations as these may prove to be good vaccine candidates. gp40/15 is a zoite antigen synthesized as a precursor pro...

journal_title：Molecular and biochemical parasitology

authors： O'Connor RM,Wanyiri JW,Cevallos AM,Priest JW,Ward HD

更新日期：2007-11-01 00:00:00

abstract：:A gene which overexpresses a 36-kDa protein (p36) in tunicamycin-resistant Leishmania was mapped by transfection and overexpression to the upstream region of the drug maker in the extrachromosomal amplicon. Complete sequencing of this region revealed a single open reading frame of about 1 kb. Authenticity of the clone...

journal_title：Molecular and biochemical parasitology

authors： Liu X,Chang KP

更新日期：1994-08-01 00:00:00

abstract：:The microtubular cytoskeleton of most single-celled eukaryotes radiates from an organizing center called the flagellar apparatus, which is essential for locomotion, feeding and reproduction. The structure of the flagellar apparatus tends to be conserved within diverse clades of eukaryotes, and modifications of this ov...

journal_title：Molecular and biochemical parasitology

authors： Yubuki N,Čepička I,Leander BS

更新日期：2016-01-01 00:00:00

abstract：:The enzyme pterin-4a-carbinolamine dehydratase (PCD) is important for the recycling of pterins within eukaryotic cells. A recombinant expression system for PCD from the apicomplexan parasite Toxoplasma gondii has been prepared, the protein purified and crystallised. Single crystal X-ray diffraction methods have produc...

journal_title：Molecular and biochemical parasitology

authors： Cameron S,Fyffe SA,Goldie S,Hunter WN

更新日期：2008-04-01 00:00:00

abstract：:An NADH-cytochrome c reductase (complex I-III) was isolated from Ascaris suum muscle mitochondria. The enzyme preparation catalyzed the reduction of 1.68 mumol cytochrome c min-1 mg-1 protein at 25 degrees C with NADH but not with NADPH, and retained its sensitivity to rotenone, piericidin A and 2-heptyl-4-hydroxyquin...

journal_title：Molecular and biochemical parasitology

authors： Takamiya S,Furushima R,Oya H

更新日期：1984-10-01 00:00:00

abstract：:Leucine aminopeptidases (LAP; EC 3.4.11.1) are a group of metalloexopeptidases, which catalyze the sequential removal of leucine amino acids from the N-termini of the polypeptides or proteins. In this study, we identified two novel genes that encode LAPs of Clonorchis sinensis (CsLAP1 and CsLAP2) and characterized the...

journal_title：Molecular and biochemical parasitology

authors： Kang JM,Ju HL,Ju JW,Sohn WM,Kim TS,Bahk YY,Hong SJ,Na BK

更新日期：2012-03-01 00:00:00

abstract：:An axenic cultivation system was used to study the differentiation of Trypanosoma brucei bloodstream forms from long slender to short stumpy-like forms. Trypanosomes in the logarithmic phase are similar to long slender bloodstream forms freshly isolated from infected mice, differing only in the rate of oxygen uptake. ...

journal_title：Molecular and biochemical parasitology

authors： Hamm B,Schindler A,Mecke D,Duszenko M

更新日期：1990-04-01 00:00:00

abstract：:Both patent and prepatent adult Hymenolepis diminuta excreted 20-hydroxyecdysone into the culture medium when maintained in vitro. Patent worms also excreted ecdysone and comparatively large quantities of unidentified immunoreactive material of a relatively apolar nature. This latter material was shown to be depleted ...

journal_title：Molecular and biochemical parasitology

authors： Mercer JG,Munn AE,Arme C,Rees HH

更新日期：1987-12-01 00:00:00

abstract：:The tachyzoites and the tissue cysts containing bradyzoites of Neospora caninum and Toxoplasma gondii, respectively, are difficult to distinguish morphologically. Specific antigens have been identified in T. gondii tachyzoites and bradyzoites, some of which are stage-specifically expressed, and different functions hav...

journal_title：Molecular and biochemical parasitology

authors： Sonda S,Fuchs N,Connolly B,Fernandez P,Gottstein B,Hemphill A

更新日期：1998-11-30 00:00:00

abstract：:The influence of growth in the presence of nifurtimox on total lipids of Trypanosoma cruzi epimastigotes was determined. Nifurtimox-treated organisms showed greater amounts of unsaturated fatty acids as compared with control cells. 18:2 was the major component in the total esterified lipid fraction. Similar results we...

journal_title：Molecular and biochemical parasitology

authors： Barreto-Bergter E,Hogge L,Steele da Cruz F

更新日期：1986-12-01 00:00:00

abstract：:The ability of Leishmania amastigotes to survive within the drastic environmental changes encountered in the phagolysosomes of mammalian macrophages is heavily dependent on the developmental regulation of a variety of genes. The identification of genes that are expressed preferentially in the mammalian stage of the pa...

journal_title：Molecular and biochemical parasitology

authors： Rochette A,McNicoll F,Girard J,Breton M,Leblanc E,Bergeron MG,Papadopoulou B

更新日期：2005-04-01 00:00:00

abstract：:Treatment of Trypanosoma cruzi blood trypomastigotes with p-chloromercuriphenylsulfonic acid (PCMS) increased the association of the parasite with either mouse resident peritoneal macrophages or rat heart myoblasts in vitro. The effect was evidenced by elevation of both the percentage of host cells with parasites and ...

journal_title：Molecular and biochemical parasitology

authors： Ayala A,Kierszenbaum F

更新日期：1987-02-01 00:00:00

abstract：:A Mo7-derived Babesia bovis line stably transfected with the gfp-bsd gene was inoculated into two four to five months old calves, while two additional calves were inoculated with Mo7 parasites. Similar mild clinical signs were detected in all calves. B. bovis rap-1 was identified in the bloodstream by PCR four days po...

journal_title：Molecular and biochemical parasitology

authors： Suarez CE,Laughery JM,Schneider DA,Sondgeroth KS,McElwain TF

更新日期：2012-09-01 00:00:00

abstract：:The karyotype and chromosomes of the human malaria parasite Plasmodium falciparum have been well characterized in recent years. Here we present karyotype maps of the three other human malaria species, P. vivax, P. malariae and P. ovale. Chromosomes of these species were found to be of significantly higher molecular we...

journal_title：Molecular and biochemical parasitology

authors： Carlton JM,Galinski MR,Barnwell JW,Dame JB

更新日期：1999-06-25 00:00:00

abstract：:Protozoan parasites of the genus Leishmania have a digenetic lifecycle, alternating between the promastigote and amastigote stages. The extracellular promastigote resides within a sandfly vector, while the obligate intracellular amastigote stage replicates in the phagolysosome of mammalian host macrophages. Adaptation...

journal_title：Molecular and biochemical parasitology

authors： Murray A,Fu C,Habibi G,McMaster WR

更新日期：2007-06-01 00:00:00

abstract：:Adenylosuccinate synthetase (AdSS) catalyses the Mg(2+) dependent formation of adenylosuccinate from IMP and aspartate, the reaction being driven by the hydrolysis of GTP to GDP. All characterized AdSS thus far exhibit a random kinetic mechanism. We present here kinetic evidence that unlike all other AdSS, Plasmodium ...

journal_title：Molecular and biochemical parasitology

authors： Raman J,Mehrotra S,Anand RP,Balaram H

更新日期：2004-11-01 00:00:00

abstract：:As genomic sequencing of Leishmania nears completion, functional analyses that provide a global genetic perspective on biological processes are important. Despite polycistronic transcription, RNA transcript abundance can be measured using microarrays. To provide a resource to evaluate cDNA arrays, we undertook 5' expr...

journal_title：Molecular and biochemical parasitology

authors： Almeida R,Gilmartin BJ,McCann SH,Norrish A,Ivens AC,Lawson D,Levick MP,Smith DF,Dyall SD,Vetrie D,Freeman TC,Coulson RM,Sampaio I,Schneider H,Blackwell JM

更新日期：2004-07-01 00:00:00

abstract：:Precipitin arcs formed after immunoelectrophoresis of Schistosoma mansoni egg, worm and cercarial antigens with polyspecific rabbit antisera have been 'stained' at neutral pH with chromogenic enzyme substrates specific for proteases and peptidases. A total of 7 antigenically distinct enzymes with peptidolytic properti...

journal_title：Molecular and biochemical parasitology

authors： Doenhoff MJ,Modha J,Curtis RH,Adeoye GO

更新日期：1988-12-01 00:00:00

abstract：:The telomeric sequence cloned from Plasmodium berghei (see M. Ponzi et al. (1985) EMBO J. 4, 2991-2995) was tested for species specificity. A telomeric and a subtelomeric fragment of the cloned insert served as separate, labelled probes on pulsed field gradient electrophoretical patterns and on genomic digests from th...

journal_title：Molecular and biochemical parasitology

authors： Dore E,Pace T,Ponzi M,Scotti R,Frontali C

更新日期：1986-11-01 00:00:00

abstract：:Plasmodium falciparum is the causative organism for the most severe form of malaria among humans. The clinical symptoms are accredited to the asexual stage of parasite life cycle, involving merozoite invasion of erythrocyte, development and re-invasion into the new erythrocyte. Interaction of parasite proteins present...

journal_title：Molecular and biochemical parasitology

authors： Hans N,Relan U,Dubey N,Gaur D,Chauhan VS

更新日期：2015-08-01 00:00:00