DNA sequence of Crithidia fasciculata kinetoplast minicircles.


:Kinetoplast DNA (kDNA) networks of the insect trypanosomatid Crithidia fasciculata strain CF-C1 contain a nearly homogeneous population of kDNA minicircles as judged by restriction enzyme cleavage analysis. We have determined the entire nucleotide sequence of the major class of minicircles by analyzing M13 phage clones carrying half-length segments of the kDNA minicircle molecules. The 12 nucleotide sequence d(G-G-G-G-T-T-G-G-T-G-T-A) is the longest sequence common to kDNA minicircles from several trypanosome species examined to date. Two copies of this universal minicircle sequence were identified 180 degrees apart as direct repeats within the C. fasciculata kDNA minicircles. In addition, these universal minicircle sequences are contained within direct repeats with nearly identical sequences of 173 and 177 base pairs (bp) in length. These sequences are also conserved in the same arrangement in minor sequence classes of minicircles from this strain. Site-specific discontinuities on both strands of the minicircle, identified previously in minicircle replication intermediates, were localized within the 173 and 177 bp conserved sequences. These sequences were also found to have extensive homology with similar conserved sequences in kDNA minicircles from Leishmania tarentolae. We suggest that the two conserved sequences, each containing a single copy of the universal minicircle sequence, represent replication origins in the Crithidia minicircles.


Mol Biochem Parasitol


Sugisaki H,Ray DS




Has Abstract


1987-04-01 00:00:00














  • Molecular cloning, organellar targeting and developmental expression of mitochondrial chaperone HSP60 in Toxoplasma gondii.

    abstract::The obligate intracellular protozoan parasite Toxoplasma gondii has a single tubular mitochondrion. During infection, it recruits the host cell's mitochondria abutting to the intracellular vacuole, that contains the parasites. The respective contribution of host and parasitic mitochondria in the intracellular growth o...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Toursel C,Dzierszinski F,Bernigaud A,Mortuaire M,Tomavo S

    更新日期:2000-12-01 00:00:00

  • 3-Methyladenine blocks Toxoplasma gondii division prior to centrosome replication.

    abstract::The apicomplexan Toxoplasma gondii replicates by endodyogeny, in which replicated organelles assemble into nascent daughter buds within the maternal parasite. The mechanisms governing this complex sequence are not understood. We now report that the kinase inhibitor 3-methlyadenine (3-MA) efficiently blocks T. gondii r...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Wang Y,Karnataki A,Parsons M,Weiss LM,Orlofsky A

    更新日期:2010-10-01 00:00:00

  • Purification and characterization of gamma-glutamylcysteine synthetase from Ascaris suum.

    abstract::We have purified and characterized the Ascaris suum gamma-glutamylcysteine synthetase, the rate-limiting step in the glutathione biosynthesis. The purified enzyme exhibited a specific activity of 18 U (mg protein)-1. Estimation of the molecular mass of the native enzyme by FPLC on Superdex S-200 revealed the presence ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hussein AS,Walter RD

    更新日期:1995-06-01 00:00:00

  • The immunodominant 17-kDa antigen from Cryptosporidium parvum is glycosylphosphatidylinositol-anchored.

    abstract::Cryptosporidium parvum is a protozoan parasite of the intestinal epithelium that has caused numerous outbreaks of diarrheal illness in humans. During our studies of the host immune response to C. parvum infection, we noted that two of the immunodominant surface antigens of the sporozoite stage of the parasite readily ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Priest JW,Xie LT,Arrowood MJ,Lammie PJ

    更新日期:2001-03-01 00:00:00

  • Sequencing and characterization of an Echinococcus multilocularis DNA probe and its use in the polymerase chain reaction.

    abstract::The nucleotide sequence of the cloned Echinococcus multilocularis DNA probe pAL1 was determined in order to simplify and improve the sensitivity of a diagnostic assay through the application of the polymerase chain reaction (PCR). The insert-specific oligonucleotides BG1 and BG2 define a 2.6-kb fragment in the genomic...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Gottstein B,Mowatt MR

    更新日期:1991-02-01 00:00:00

  • The cyst wall of Entamoeba invadens contains chitosan (deacetylated chitin).

    abstract::The cyst wall of Entamoeba invadens (Ei), a model for the human pathogen Entamoeba histolytica, contains chitin, which is a homopolymer of beta-1, 4-linked N-acetyl-glucosamine (GlcNAc). In fungi and in bacteria that make nodulation factors, chitin deacetylases make chitosan, which is a mixture of GlcNAc and glucosami...

    journal_title:Molecular and biochemical parasitology

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    authors: Das S,Van Dellen K,Bulik D,Magnelli P,Cui J,Head J,Robbins PW,Samuelson J

    更新日期:2006-07-01 00:00:00

  • Purification and properties of Plasmodium falciparum malate dehydrogenase.

    abstract::Asexual intraerythrocytic Plasmodium falciparum were shown to have a single isoenzyme of malate dehydrogenase. This malate dehydrogenase was purified to apparent homogeneity using a three-step purification protocol. The parasite malate dehydrogenase had an apparent subunit molecular weight of 32 kDa, a pH optimum of 7...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Lang-Unnasch N

    更新日期:1992-01-01 00:00:00

  • Protoporphyrinogen IX oxidase from Plasmodium falciparum is anaerobic and is localized to the mitochondrion.

    abstract::Earlier studies in this laboratory had shown that the malarial parasite can synthesize heme de novo and inhibition of the pathway leads to death of the parasite. It has been proposed that the pathway for the biosynthesis of heme in Plasmodium falciparum is unique involving three different cellular compartments, namely...

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    pub_type: 杂志文章


    authors: Nagaraj VA,Arumugam R,Prasad D,Rangarajan PN,Padmanaban G

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  • Mechanisms of host seeking by parasitic nematodes.

    abstract::The phylum Nematoda comprises a diverse group of roundworms that includes parasites of vertebrates, invertebrates, and plants. Human-parasitic nematodes infect more than one billion people worldwide and cause some of the most common neglected tropical diseases, particularly in low-resource countries [1]. Parasitic nem...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Gang SS,Hallem EA

    更新日期:2016-07-01 00:00:00

  • Multimerization of the Toxoplasma gondii MIC2 integrin-like A-domain is required for binding to heparin and human cells.

    abstract::Toxoplasma gondii is an obligate intracellular parasite that causes toxoplasmosis in humans and animals. To invade host cells, T. gondii deploys the contents of its apically oriented secretory organelles that include the micronemes. Contained within the micronemes are proteins that possess adhesive motifs resembling t...

    journal_title:Molecular and biochemical parasitology

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    authors: Harper JM,Hoff EF,Carruthers VB

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  • Peptide-fluoromethyl ketones arrest intracellular replication and intercellular transmission of Trypanosoma cruzi.

    abstract::The major proteolytic activity of Trypanosoma cruzi is a cathepsin L-like cysteine protease expressed in all stages of the parasite. As an initial step in identifying possible functions of this enzyme in the life cycle of T. cruzi, and examining its potential as a target for rational drug design, two fluoromethyl keto...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Harth G,Andrews N,Mills AA,Engel JC,Smith R,McKerrow JH

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  • Presence of contaminating mitochondrial DNA from host reticulocytes in experimental infections of Plasmodium berghei.

    abstract::Superposition of two unrelated processes, namely terminal reticulocyte differentiation and synchronous plasmodial development, takes place in experimental infections of Plasmodium berghei. The first process is shown to be responsible for the appearance of some discrete restriction bands of host origin when DNA is extr...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Pace T,Ponzi M,Dore E,Scotti R,Mons B

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  • RNA interference targeting leucine aminopeptidase blocks hatching of Schistosoma mansoni eggs.

    abstract::Schistosoma mansoni leucine aminopeptidase (LAP) is thought to play a central role in hatching of the miracidium from the schistosome egg. We identified two discrete LAPs genes in the S. mansoni genome, and their orthologs in S. japonicum. The similarities in sequence and exon/intron structure of the two genes, LAP1 a...

    journal_title:Molecular and biochemical parasitology

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    authors: Rinaldi G,Morales ME,Alrefaei YN,Cancela M,Castillo E,Dalton JP,Tort JF,Brindley PJ

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  • Sequence preference of chloroquine binding to DNA and prevention of Z-DNA formation.

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kwakye-Berko F,Meshnick S

    更新日期:1990-03-01 00:00:00

  • Choline transport in Leishmania major promastigotes and its inhibition by choline and phosphocholine analogs.

    abstract::Phosphatidylcholine is the most abundant phospholipid in the membranes of the human parasite Leishmania. The metabolic pathways leading to its biosynthesis are likely to play a critical role in parasite development and survival and may offer a good target for antileishmanial chemotherapy. Phosphatidylcholine synthesis...

    journal_title:Molecular and biochemical parasitology

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    authors: Zufferey R,Mamoun CB

    更新日期:2002-11-01 00:00:00

  • Linearized free maxicircle DNA in Crithidia fasciculata is a product of topoisomerase II-mediated cleavage.

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    journal_title:Molecular and biochemical parasitology

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    authors: Carpenter LR,Englund PT

    更新日期:1996-02-01 00:00:00

  • Interallelic recombination in the merozoite surface protein 1 (MSP-1) gene of Plasmodium vivax from Thai isolates.

    abstract::The merozoite of Plasmodium vivax possesses a high molecular mass surface protein called Pv-merozoite surface protein 1, PvMSP-1, which exhibits antigenic diversity among isolates. In this study, the extent of sequence variation in the polymorphic region and the flanking interspecies conserved blocks (ICBs) 5 and 6 of...

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    authors: Putaporntip C,Jongwutiwes S,Tanabe K,Thaithong S

    更新日期:1997-01-01 00:00:00

  • Cysteine proteinase of Entamoeba histolytica. I. Partial purification and action on different enzymes.

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    journal_title:Molecular and biochemical parasitology

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    authors: Scholze H,Werries E

    更新日期:1986-01-01 00:00:00

  • Translation initiation factors GleIF4E2 and GleIF4A can interact directly with the components of the pre-initiation complex to facilitate translation initiation in Giardia lamblia.

    abstract::Translation initiation factor eIF4F is essential for cap-dependent translation initiation in eukaryotes. eIF4F is a trimeric complex consisting of a scaffold protein eIF4G, cap-binding protein eIF4E and DEAD-box RNA helicase eIF4A. eIF4F binds to the 5' cap structure of the mRNA through eIF4E and facilitates the bindi...

    journal_title:Molecular and biochemical parasitology

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Pampori NA,Singh G,Srivastava VM

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  • In vivo 31P NMR spectrum of Hymenolepis diminuta and its change on short-term exposure to mebendazole.

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    journal_title:Molecular and biochemical parasitology

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    authors: Thompson SN,Platzer EG,Lee RW

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  • Molecular characterization of the alpha-subunit of Trichomonas vaginalis hydrogenosomal succinyl CoA synthetase.

    abstract::The anaerobic, parasitic protist, Trichomonas vaginalis, is characterized by the absence of mitochondria and the presence of double membrane bound organelles called hydrogenosomes. Succinyl-coenzyme A synthetase is a hydrogenosomal enzyme which catalyzes the formation of ATP via substrate-level phosphorylation. We hav...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


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    更新日期:1994-08-01 00:00:00

  • Is point mutagenesis a mechanism for antigenic variation in Trypanosoma brucei?

    abstract::Antigenic variation in African trypanosomes proceeds by switching between different variant surface glycoprotein (VSG) molecules, whose extensive epitope differences enable evasion of antibody responses. Each trypanosome has approximately 1000 basic copy VSG genes inside chromosomes and a subset located at telomeres. ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Graham VS,Barry JD

    更新日期:1996-07-01 00:00:00

  • Structural features affecting variant surface glycoprotein expression in Trypanosoma brucei.

    abstract::The glycosylphosphatidylinositol (GPI)-anchored variant surface glycoprotein (VSG) of Trypanosoma brucei is the most abundant GPI-anchored protein expressed on any cell, and is an essential virulence factor. To determine what structural features affect efficient expression of VSG, we made a series of mutations in two ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Wang J,Böhme U,Cross GA

    更新日期:2003-05-01 00:00:00

  • Characterization of beta-tubulin genes in hookworms and investigation of resistance-associated mutations using real-time PCR.

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Schwenkenbecher JM,Albonico M,Bickle Q,Kaplan RM

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  • Structure and function of Per-ARNT-Sim domains and their possible role in the life-cycle biology of Trypanosoma cruzi.

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Rojas-Pirela M,Rigden DJ,Michels PA,Cáceres AJ,Concepción JL,Quiñones W

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    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Alphey L,Andreasen M

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  • Isolation and characterization of infective and non-infective clones of Leishmania tropica.

    abstract::Cloning by limit dilution of an isolate of Leishmania tropica (LRC-L137) that is infective for mice resulted in 7 stable clones, only one of which was infective in BALB/c mice. Three of the non-infective clones that were examined for survival in BALB/c macrophages in vitro seemed to be killed more readily, suggesting ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Handman E,Hocking RE,Mitchell GF,Spithill TW

    更新日期:1983-02-01 00:00:00

  • Characterisation of the loci encoding the glutamic acid and alanine rich protein of Trypanosoma congolense.

    abstract::We have characterised the organisation of genes encoding the glutamate and alanine rich protein (GARP) surface coat of the procyclic and epimastigote stages of Trypanosoma congolense in the tsetse fly. The GARP genes are arranged at two, possibly physically linked, loci, one of which exhibits allelic variation. One lo...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Rangarajan D,Harvey TI,Barry JD

    更新日期:2000-02-05 00:00:00

  • TbUTP10, a protein involved in early stages of pre-18S rRNA processing in Trypanosoma brucei.

    abstract::Ribosome biosynthesis, best studied in opisthokonts, is a highly complex process involving numerous protein and RNA factors. Yet, very little is known about the early stages of pre-18S rRNA processing even in these model organisms, let alone the conservation of this mechanism in other eukaryotes. Here we extend our kn...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Faktorová D,Bär A,Hashimi H,McKenney K,Horák A,Schnaufer A,Rubio MAT,Alfonzo JD,Lukeš J

    更新日期:2018-10-01 00:00:00