Structural analysis of the acetaldehyde dehydrogenase activity of Entamoeba histolytica alcohol dehydrogenase 2 (EhADH2), a member of the ADHE enzyme family.


:The ADHE family of enzymes are bifunctional acetaldehyde dehydrogenase (ALDH)/alcohol dehydrogenase (ADH) enzymes that probably arose from the fusion of genes encoding separate ALDH and ADH enzymes. Here we have used the Entamoeba histolytica alcohol dehydrogenase 2 (EhADH2) enzyme as a prototype to analyze the structure and function of the ALDH domain of ADHE enzymes. We find that the N-terminal domain of EhADH2, encompassing amino acids 1-446, is sufficient for ALDH activity, consistent with the concept that EhADH2, and other members of the ADHE family comprise fusion peptides. In addition, we show, using site directed mutagenesis, that the catalytic mechanism for the ALDH activity appears to be similar to that described for other members of the ALDH extended family.


Mol Biochem Parasitol


Chen M,Li E,Stanley SL Jr




Has Abstract


2004-10-01 00:00:00














  • Sequence preference of chloroquine binding to DNA and prevention of Z-DNA formation.

    abstract::Chloroquine is a critically important antimalarial drug and a well known intercalator into DNA. We now show that chloroquine binds more avidly to poly(dG-dC).poly(dG-dC) than to other synthetic polynucleotides and that this binding inhibits both salt- and cobalt-induced transitions to Z-DNA. These data are consistent ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kwakye-Berko F,Meshnick S

    更新日期:1990-03-01 00:00:00

  • Acidic phosphoproteins associated with the host erythrocyte membrane of erythrocytes infected with Plasmodium berghei and P. chabaudi.

    abstract::New phosphoproteins appear on the host erythrocyte membrane during Plasmodium berghei and P. chabaudi infection. Distinct proteins having similar properties and all distinguished by isoelectric points of less than 4.0 are identified. Associated with the erythrocyte membranes of P. berghei infected erythrocytes are two...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Wiser MF,Leible MB,Plitt B

    更新日期:1988-01-01 00:00:00

  • Structural features affecting variant surface glycoprotein expression in Trypanosoma brucei.

    abstract::The glycosylphosphatidylinositol (GPI)-anchored variant surface glycoprotein (VSG) of Trypanosoma brucei is the most abundant GPI-anchored protein expressed on any cell, and is an essential virulence factor. To determine what structural features affect efficient expression of VSG, we made a series of mutations in two ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Wang J,Böhme U,Cross GA

    更新日期:2003-05-01 00:00:00

  • Purification and characterization of three larval excretory-secretory proteins of Dirofilaria immitis.

    abstract::Two proteins were previously described in the excretory-secretory products (ES) collected from Dirofilaria immitis during the molt from the third stage to the fourth stage in vitro. The two proteins were purified using cation exchange and reverse phase HPLC. During the purification of these two proteins, a third prote...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Frank GR,Grieve RB

    更新日期:1996-01-01 00:00:00

  • Expression of a stage-specific lipophosphoglycan in Leishmania major amastigotes.

    abstract::Amastigotes of Leishmania major were isolated from infected mice and radiolabeled for 2 h with [3H]galactose. An acidic [3H]glycoconjugate was extracted from a dilipidated residue fraction with the solvent water/ethanol/diethylether/pyridine/NH4OH (15:15:5:1:0.017). The radioactivity labeled glycoconjugate was found t...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Turco SJ,Sacks DL

    更新日期:1991-03-01 00:00:00

  • Identification of novel splice variants of the voltage- and Ca²⁺-dependent K⁺-channel SLO-1 of Trichuris muris.

    abstract::The anthelmintic cyclooctadepsipeptide emodepside is effective against nematodes showing resistance against established drug classes. Emodepside exerts its nematicidal effects mainly through its validated target, the tetrameric voltage- and calcium-activated potassium channel SLO-1. Two slo-1 genes were described in T...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Yilmaz E,Kulke D,von Samson-Himmelstjerna G,Krücken J

    更新日期:2015-01-01 00:00:00

  • Ecdysteroid excretion by adult Hymenolepis diminuta in vitro.

    abstract::Both patent and prepatent adult Hymenolepis diminuta excreted 20-hydroxyecdysone into the culture medium when maintained in vitro. Patent worms also excreted ecdysone and comparatively large quantities of unidentified immunoreactive material of a relatively apolar nature. This latter material was shown to be depleted ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Mercer JG,Munn AE,Arme C,Rees HH

    更新日期:1987-12-01 00:00:00

  • Three genes and two isozymes: gene conversion and the compartmentalization and expression of the phosphoglycerate kinases of Trypanosoma (Nannomonas) congolense.

    abstract::The glycosome, a microbody organelle found only in kinetoplastid protozoa, compartmentalizes the first six enzymes of glycolysis. In order to better understand the regulation and targeting of glycolytic enzymes in trypanosomes, we have cloned and analyzed the three genes of the phosphoglycerate kinase (PGK) complex of...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Parker HL,Hill T,Alexander K,Murphy NB,Fish WR,Parsons M

    更新日期:1995-02-01 00:00:00

  • Structural studies on Plasmodium vivax merozoite surface protein-1.

    abstract::Plasmodium vivax infection is the second most common cause of malaria throughout the world. Like other Plasmodium species, P. vivax has a large protein complex, MSP-1, located on the merozoite surface. The C-terminal MSP-1 sub-unit, MSP-1(42), is cleaved during red blood cell invasion, causing the majority of the comp...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Babon JJ,Morgan WD,Kelly G,Eccleston JF,Feeney J,Holder AA

    更新日期:2007-05-01 00:00:00

  • L-myo-Inositol 1-phosphate synthase from Entamoeba histolytica.

    abstract::L-myo-Inositol 1-phosphate synthase (I-1-P synthase) catalyses the primary reaction for the synthesis of inositol in a variety of prokaryotes, eukaryotes and in the chloroplasts of algae and higher plants. Inositol is a precursor of essential macromolecules like membrane phospholipids, GPI anchor proteins and lipophos...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Lohia A,Hait NC,Majumder AL

    更新日期:1999-01-05 00:00:00

  • RNAi interference of XPO1 and Sm genes and their effect on the spliced leader RNA in Trypanosoma brucei.

    abstract::In trypanosomes, trans-splicing is a major essential RNA-processing mechanism that involves the addition of a spliced leader sequence to all mRNAs from a small RNA species, known as the spliced leader RNA (SL RNA). SL RNA maturation is poorly understood and it is not clear where assembly with Sm proteins takes place. ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Biton M,Mandelboim M,Arvatz G,Michaeli S

    更新日期:2006-12-01 00:00:00

  • A bioinformatic analysis of the RAB genes of Trypanosoma brucei.

    abstract::RAB proteins are small GTPases with vital roles in eukaryotic intracellular transport; orthologous RABs appear to fulfil similar functions in diverse organisms. Trypanosoma brucei spp., the causative organisms of Old World trypanosomiasis of humans and domestic animals, have extremely effective endocytic and exocytic ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ackers JP,Dhir V,Field MC

    更新日期:2005-05-01 00:00:00

  • Characterization of a SR protein from Trypanosoma brucei with homology to RNA-binding cis-splicing proteins.

    abstract::The protozoan parasite Trypanosoma brucei relies on trans-splicing to process its mRNAs. A novel nuclear serine/arginine (SR)-rich trypanosomal protein (TSR1) was characterized which contains two RNA recognition motifs. The TSR1 protein appears to be homologous to RNA-binding SR proteins of the cis-splicing machinery ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ismaïli N,Pérez-Morga D,Walsh P,Mayeda A,Pays A,Tebabi P,Krainer AR,Pays E

    更新日期:1999-07-30 00:00:00

  • Studies on mitochondrial ATPase of Leishmania donovani using digitonin-permeabilized promastigotes.

    abstract::Mitochondrial ATPase of Leishmania donovani was characterized using digitonin-permeabilized promastigotes and the results were compared with those from isolated mitochondria. Maximum mitochondrial ATPase activity was obtained in promastigotes permeabilized with digitonin at a final concentration of 20 microM and the s...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Das A

    更新日期:1993-08-01 00:00:00

  • Trypanosoma cruzi attachment to lymphocyte muscarinic cholinergic and beta adrenergic receptors modulates intracellular signal transduction.

    abstract::Plasma membrane vesicles of Trypanosoma cruzi (PMVs) formed saturation binding isotherms with naive murine T lymphocytes. Parasite membrane attachment to the muscarinic cholinergic receptors of Lyt 2.2+T cells (suppressor cells) resulted in the synthesis of cGMP, attenuation of cAMP levels and in the secretion of pros...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Borda ES,Sterin-Borda LJ,Pascual JO,Gorelik G,Felix JC,von Kreuter BF,Santos-Buch CA

    更新日期:1991-07-01 00:00:00

  • Characterisation of protein kinase C like activities in Trypanosoma brucei.

    abstract::Protein kinase activities in bloodstream and procyclic forms of Trypanosoma brucei have been partially purified and characterised. Cytosolic extracts were separated on DEAE-cellulose and assayed for the ability to phosphorylate histone in the presence of Ca2+ and diacylglycerol. Five peaks of activity were identified ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Keith K,Hide G,Tait A

    更新日期:1990-11-01 00:00:00

  • The effects of p-chloromercuriphenylsulfonic acid on Trypanosoma cruzi infection of mammalian host cells in vitro.

    abstract::Treatment of Trypanosoma cruzi blood trypomastigotes with p-chloromercuriphenylsulfonic acid (PCMS) increased the association of the parasite with either mouse resident peritoneal macrophages or rat heart myoblasts in vitro. The effect was evidenced by elevation of both the percentage of host cells with parasites and ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ayala A,Kierszenbaum F

    更新日期:1987-02-01 00:00:00

  • Thiol groups on the surface of anaerobic parasitic protozoa.

    abstract::Evidence is presented that Giardia lamblia and Entamoeba histolytica, phylogenetically unrelated aerotolerant anaerobes, have crucial thiol groups on or easily accessible to their external surface. Both parasites were killed by three structurally unrelated thiol-blocking reagents which penetrate intact cells poorly or...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Gillin FD,Reiner DS,Levy RB,Henkart PA

    更新日期:1984-09-01 00:00:00

  • Leishmania donovani PP2C: Kinetics, structural attributes and in vitro immune response.

    abstract::Most of the signaling pathways are regulated by reversible phosphorylation-dephosphorylation which involves enzymes- kinases and phosphatases. Current knowledge about the protein phosphatases in parasites like Trypanosoma and Leishmania is very minimal despite their enormousity. In present study, full length ORF of Le...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Jakkula P,Qureshi R,Iqbal A,Sagurthi SR,Qureshi IA

    更新日期:2018-07-01 00:00:00

  • 5'-p-Fluorosulfonyl benzoyl adenosine inhibits an ecto-ATP-diphosphohydrolase in the tegument surface of Taenia crassiceps cysticerci.

    abstract::The tegumental membrane of Taenia crassiceps cysticerci contains an ATP-diphosphohydrolase (EC which hydrolyzes purine and pyrimidine nucleoside 5'-di- and 5'-triphosphates at an optimum pH of 8.5. It is Mg(2+)-dependent and insensitive to classical ATPase and phosphatase inhibitors. In solubilized tegumental...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Guevara-Flores A,Olvera-Sánchez S,Gómez-Concha C,Juárez O,Esparza-Perusquía M,Pardo JP,Mendoza-Hernández G,Martínez F,Flores-Herrera O

    更新日期:2008-12-01 00:00:00

  • Molecular cloning, expression, and characterization of UDP N-acetyl-α-d-galactosamine: Polypeptide N-acetylgalactosaminyltransferase 4 from Cryptosporidium parvum.

    abstract::Cryptosporidium spp. are the causative agents of diarrheal disease worldwide, but effective treatments are lacking. Cryptosporidium employs mucin-like glycoproteins with O-glycans to attach to and infect host intestinal epithelial cells. The Tn antigen (GalNAcα1-Ser/Thr) is an O-glycan essential for these processes, a...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: DeCicco RePass MA,Bhat N,Heimburg-Molinaro J,Bunnell S,Cummings RD,Ward HD

    更新日期:2018-04-01 00:00:00

  • Characterisation of benzimidazole binding with recombinant tubulin from Giardia duodenalis, Encephalitozoon intestinalis, and Cryptosporidium parvum.

    abstract::The binding kinetics of several benzimidazole compounds were determined with recombinant tubulin from benzimidazole-sensitive and -insensitive organisms. This study utilised the naturally occurring high efficacy of the benzimidazoles for the parasitic protozoa Giardia duodenalis and Encephalitozoon intestinalis, and l...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: MacDonald LM,Armson A,Thompson AR,Reynoldson JA

    更新日期:2004-11-01 00:00:00

  • Isolation and substrate specificity of an adenine nucleoside phosphorylase from adult Schistosoma mansoni.

    abstract::An adenine nucleoside phosphorylase (ANP, EC none) activity was identified and partially purified from extracts of Schistosoma mansoni by chromatofocussing column chromatography and molecular sieving. The enzyme is distinct from purine nucleoside phosphorylase (PNP, EC ANP is specific for adenine nucleosides...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Savarese TM,El Kouni MH

    更新日期:2014-03-01 00:00:00

  • Characterization of the gene family encoding a host-protective antigen of the tapeworm Taenia ovis.

    abstract::Genomic structure has been determined for a gene encoding a host-protective antigen of the parasitic platyhelminth Taenia ovis. An incomplete cDNA, known as 45W, encodes the protective antigen. Southern hybridisation experiments using 45W cDNA as a probe, revealed that the 45W gene was a member of a multigene family. ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Waterkeyn JG,Lightowlers MW,Coppel R,Cowman AF

    更新日期:1995-07-01 00:00:00

  • Further characterisation of the Schistosoma japonicum protein Sj23, a target antigen of an immunodiagnostic monoclonal antibody.

    abstract::Sj23, the 23-kDa target antigen in Schistosoma japonicum adult worms of the hybridoma monoclonal antibody (mAb) I-134, has been identified and cloned from cDNA libraries, mAb I-134 has been successfully used in immunodiagnostic assays to detect S. japonicum infection in Philippine patients. Sequence analysis has shown...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Davern KM,Wright MD,Herrmann VR,Mitchell GF

    更新日期:1991-09-01 00:00:00

  • Biochemical analysis, gene structure and localization of the 24 kDa glutathione S-transferase from Onchocerca volvulus.

    abstract::Survival of Onchocerca volvulus, a pathogenic human filarial parasite, is likely to depend upon the detoxification activities of the glutathione S-transferases (GSTs). The 24 kDa O. volvulus GST, OvGST2, was expressed in a bacterial system and the recombinant protein was purified to homogeneity by affinity chromatogra...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Liebau E,Wildenburg G,Brophy PM,Walter RD,Henkle-Dührsen K

    更新日期:1996-09-01 00:00:00

  • Secretory products of helminth parasites as immunomodulators.

    abstract::Parasitic helminths release molecules into their environment, which are generally referred to as excretory-secretory products or ES. ES derived from a wide range of nematodes, trematodes and cestodes have been studied during the past 30-40 years, their characterization evolving from simple biochemical procedures such ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Harnett W

    更新日期:2014-07-01 00:00:00

  • A conserved domain targets exported PHISTb family proteins to the periphery of Plasmodium infected erythrocytes.

    abstract::During blood-stage infection, malaria parasites export numerous proteins to the host erythrocyte. The Poly-Helical Interspersed Sub-Telomeric (PHIST) proteins are an exported family that share a common 'PRESAN' domain, and include numerous members in Plasmodium falciparum, Plasmodium vivax and Plasmodium knowlesi. In ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Tarr SJ,Moon RW,Hardege I,Osborne AR

    更新日期:2014-08-01 00:00:00

  • Chemiluminescence enhancement by trypanocidal drugs and by inhibitors of antioxidant enzymes in Trypanosoma cruzi.

    abstract::The spontaneous emission of chemiluminescence by Trypanosoma cruzi epimastigotes was 133 +/- 5 counts s-1 (mg protein)-1. The measured intracellular steady state concentration of hydrogen peroxide in the same cells was 1.5 +/- 0.5 microM. These two values are about 12- and 15-times higher than the corresponding ones f...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Giulivi C,Turrens JF,Boveris A

    更新日期:1988-09-01 00:00:00

  • Characterization of a candidate gene for GP72, an insect stage-specific antigen of Trypanosoma cruzi.

    abstract::The Trypanosoma cruzi insect stage-specific antigen GP72 was purified from epimastigotes and the amino acid sequences of peptide fragments determined. Oligonucleotides derived from these data were used to amplify and clone a cDNA sequence, which was used to isolate a full-length gene. All the sequenced peptides were e...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Cooper R,Inverso JA,Espinosa M,Nogueira N,Cross GA

    更新日期:1991-11-01 00:00:00