Structural analysis of the acetaldehyde dehydrogenase activity of Entamoeba histolytica alcohol dehydrogenase 2 (EhADH2), a member of the ADHE enzyme family.

abstract：:Cloned DNA fragments of the ribosomal RNA gene of Schistosoma mansoni hybridise strongly to Echinococcus DNA following restriction endonuclease and Southern transfer analysis. Individuals within a strain of E. granulosus exhibit identical patterns of hybridisation. However, the hybridisation patterns show significant ...

journal_title：Molecular and biochemical parasitology

authors： McManus DP,Simpson AJ

更新日期：1985-11-01 00:00:00

abstract：:Both the alpha- and beta-tubulin genes of Leishmania enriettii are encoded by mRNA of 2.0-2.2 kb in length. We have shown previously that the alpha- and beta-tubulin genes are arranged in separate, tandem repeats of 2 and 4 kb, respectively, and now report the mapping of mature mRNA onto these cloned genes. Here we sh...

journal_title：Molecular and biochemical parasitology

authors： Landfear SM,Wirth DF

更新日期：1985-04-01 00:00:00

abstract：:Apicomplexa parasites use complex cell cycles to replicate that are not well understood mechanistically. We have established a robust forward genetic strategy to identify the essential components of parasite cell division. Here we describe a novel temperature sensitive Toxoplasma strain, mutant 13-20C2, which growth a...

journal_title：Molecular and biochemical parasitology

authors： Suvorova ES,Lehmann MM,Kratzer S,White MW

更新日期：2012-01-01 00:00:00

abstract：:A screen for Leishmania mexicana genes encoding promastigote-specific flagellar proteins resulted in isolation of genes encoding the major components of the paraflagellar rod. One of these, PFR-2, was characterized extensively. PFR-2 genes are present in the genome as a tandem array of three genes designated PFR-2A, P...

journal_title：Molecular and biochemical parasitology

authors： Moore LL,Santrich C,LeBowitz JH

更新日期：1996-10-01 00:00:00

abstract：:Earlier studies in this laboratory had shown that the malarial parasite can synthesize heme de novo and inhibition of the pathway leads to death of the parasite. It has been proposed that the pathway for the biosynthesis of heme in Plasmodium falciparum is unique involving three different cellular compartments, namely...

journal_title：Molecular and biochemical parasitology

authors： Nagaraj VA,Arumugam R,Prasad D,Rangarajan PN,Padmanaban G

更新日期：2010-11-01 00:00:00

abstract：:Superposition of two unrelated processes, namely terminal reticulocyte differentiation and synchronous plasmodial development, takes place in experimental infections of Plasmodium berghei. The first process is shown to be responsible for the appearance of some discrete restriction bands of host origin when DNA is extr...

journal_title：Molecular and biochemical parasitology

authors： Pace T,Ponzi M,Dore E,Scotti R,Mons B

更新日期：1989-11-01 00:00:00

abstract：:Recombinant proteins and synthetic peptides representing various sequences of gp82, a surface glycoprotein of Trypanosoma cruzi metacyclic trypomastigotes implicated in mammalian cell invasion, were used in this study aiming at the identification of the domain(s) of this molecule required for interaction with target c...

journal_title：Molecular and biochemical parasitology

authors： Santori FR,Dorta ML,Juliano L,Juliano MA,da Silveira JF,Ruiz RC,Yoshida N

更新日期：1996-06-01 00:00:00

abstract：:We have identified a GM2-activator protein (GM2AP) with highly unusual properties secreted by the nematode parasite Trichinella spiralis. Expression in Pichia pastoris resulted in a hyperglycosylated protein of 28 kDa, but the 18 kDa native protein was not glycosylated. The parasite GM2AP does not facilitate degradati...

journal_title：Molecular and biochemical parasitology

authors： Bruce AF,Gares MP,Selkirk ME,Gounaris K

更新日期：2006-06-01 00:00:00

abstract：:Four Trypanosoma cruzi genomic DNA fragments carrying different TcP2 beta genes have been isolated and sequenced. Three of them had a single TcP2 beta gene, while the 3.8-kb-long DNA segment encoding the TcP2 beta-H1.8 locus showed two TcP2 beta genes arranged in tandem. These genes were physically connected by a 428-...

journal_title：Molecular and biochemical parasitology

authors： Vazquez MP,Schijman AG,Levin MJ

更新日期：1994-04-01 00:00:00

abstract：:Malaria parasites export 'a secretome' of hundreds of proteins, including major virulence determinants, from their endoplasmic reticulum (ER), past the parasite plasma and vacuolar membranes to the host erythrocyte. The export mechanism is high affinity (nanomolar) binding of a host (cell) targeting (HT) motif RxLxE/D...

journal_title：Molecular and biochemical parasitology

authors： Bhattacharjee S,Speicher KD,Stahelin RV,Speicher DW,Haldar K

更新日期：2012-10-01 00:00:00

abstract：:Genetic transformation of malaria parasites has been limited by the number of selectable markers available. For the rodent malaria parasite, Plasmodium berghei, only a single selection marker has been at hand, utilising the dihydrofolate reductase-thymidylate synthase gene from either P. berghei or Toxoplasma gondii t...

journal_title：Molecular and biochemical parasitology

authors： de Koning-Ward TF,Fidock DA,Thathy V,Menard R,van Spaendonk RM,Waters AP,Janse CJ

更新日期：2000-03-05 00:00:00

abstract：:Three days after mice were inoculated with Trypanosoma brucei gambiense, total hepatic cytochrome P-450 levels were decreased 14% from control values, while the specific mixed-function oxidase activity (per nmole of cytochrome P-450) was inhibited almost 40% in infected animals. Furthermore, drugs used to treat trypan...

journal_title：Molecular and biochemical parasitology

authors： Shertzer HG,Hall JE,Seed JR

更新日期：1981-08-01 00:00:00

abstract：:Plasmodium falciparum trophozoites were isolated by mechanical rupture of infected human erythrocytes followed by a series of differential centrifugation steps. After lysis with sonication, the 100 000 x g supernatant of parasites and uninfected host cells was used to determine the specific activities of a number of e...

journal_title：Molecular and biochemical parasitology

authors： Reyes P,Rathod PK,Sanchez DJ,Mrema JE,Rieckmann KH,Heidrich HG

更新日期：1982-05-01 00:00:00

abstract：:Toxocariasis is a neglected disease, and its main etiological agent is the nematode Toxocara canis. Serological diagnosis is performed by an enzyme-linked immunosorbent assay using T. canis excretory and secretory (TES) antigens produced by in vitro cultivation of larvae. Identification of TES proteins can be useful f...

journal_title：Molecular and biochemical parasitology

authors： Sperotto RL,Kremer FS,Aires Berne ME,Costa de Avila LF,da Silva Pinto L,Monteiro KM,Caumo KS,Ferreira HB,Berne N,Borsuk S

更新日期：2017-01-01 00:00:00

abstract：:Two proteins were previously described in the excretory-secretory products (ES) collected from Dirofilaria immitis during the molt from the third stage to the fourth stage in vitro. The two proteins were purified using cation exchange and reverse phase HPLC. During the purification of these two proteins, a third prote...

journal_title：Molecular and biochemical parasitology

authors： Frank GR,Grieve RB

更新日期：1996-01-01 00:00:00

abstract：:The nucleotide sequence of a gene encoding a 35-kDa thiol protease of the parasitic nematode Haemonchus contortus has been determined. The gene, designated AC-2, shares 97% nucleotide sequence identity and 98% amino acid identity with previously characterized AC-1 cDNAs encoding the thiol protease. The AC-2 gene spans...

journal_title：Molecular and biochemical parasitology

authors： Pratt D,Cox GN,Milhausen MJ,Boisvenue RJ

更新日期：1990-12-01 00:00:00

abstract：:Entamoeba histolytica, an enteric protozoan parasite, infects 10% of the world's population leading to 50 million cases of invasive amoebiasis annually. Parasite vesicle trafficking and motility, which relies on vesicle trafficking to deliver membrane and membrane components to the leading edge, are important for viru...

journal_title：Molecular and biochemical parasitology

authors： Welter BH,Temesvari LA

更新日期：2004-06-01 00:00:00

abstract：:Strongyloides and related genera are advantageous subjects for transgenesis in parasitic nematodes, primarily by gonadal microinjection as has been used with Caenorhabditis elegans. Transgenesis has been achieved in Strongyloides stercoralis and in Parastrongyloides trichosuri, but both of these lack well-adapted, con...

journal_title：Molecular and biochemical parasitology

authors： Li X,Shao H,Junio A,Nolan TJ,Massey HC Jr,Pearce EJ,Viney ME,Lok JB

更新日期：2011-10-01 00:00:00

abstract：:A protease from Plasmodium falciparum was purified 150 fold by high performance liquid chromatography on a TSK-G-3000 SW exclusion column. The enzyme is not retained during pressure filtration with an Amicon PM10 membrane but is retained by a YM5 membrane. The molecular weight of the protease is less than 10,000, base...

journal_title：Molecular and biochemical parasitology

authors： Vander Jagt DL,Hunsaker LA,Campos NM

更新日期：1986-03-01 00:00:00

abstract：:The cysteine proteinase B of Leishmania parasites is an important virulence factor. In this study we have expressed, isolated and characterized for the first time a recombinant CPB from Leishmania braziliensis, the causative agent of mucocutaneous leishmaniosis. The mature region of the recombinant CPB shares a high p...

journal_title：Molecular and biochemical parasitology

authors： Lanfranco MF,Loayza-Muro R,Clark D,Núñez R,Zavaleta AI,Jimenez M,Meldal M,Coombs GH,Mottram JC,Izidoro M,Juliano MA,Juliano L,Arévalo J

更新日期：2008-10-01 00:00:00

abstract：:Trypanosoma brucei is a unicellular parasite that is transmitted from one mammalian host to the next by tsetse flies. The expression of many trypanosome genes is regulated during the life cycle but there is no evidence for developmental control of transcription by RNA polymerase II. T. brucei expresses at least two he...

journal_title：Molecular and biochemical parasitology

authors： Hotz HR,Lorenz P,Fischer R,Krieger S,Clayton C

更新日期：1995-12-01 00:00:00

abstract：:A Babesia bovis merozoite protein, Bb-1, was localized by immunoelectron microscopy to an apical organelle known as the spherical body. This unique structure appears to be analogous to dense granules of other apicomplexan protozoa. Similar to previously described dense granule proteins of Plasmodium spp., Bb-1 is secr...

journal_title：Molecular and biochemical parasitology

authors： Hines SA,Palmer GH,Brown WC,McElwain TF,Suarez CE,Vidotto O,Rice-Ficht AC

更新日期：1995-02-01 00:00:00

abstract：:Previous studies in the parasitic protist Trichomonas vaginalis have revealed that protein coding genes are transcribed by an alpha-amanitin-resistant RNA polymerase (RNAP) II. To investigate whether this unusual property is a general characteristic of trichomonads, we addressed the physiology of RNA synthesis in lyso...

journal_title：Molecular and biochemical parasitology

authors： Vanácová S,Tachezy J,Ullu E,Tschudi C

更新日期：2001-07-01 00:00:00

abstract：:Adults of Onchocerca volvulus and Onchocerca gibsoni were identically fractionated into a surface-enriched fraction, a phosphate buffered saline (PBS) extract and a PBS insoluble-detergent soluble fraction. Glycoproteins were prepared from these extracts and all fractions were examined by the Western blot technique us...

journal_title：Molecular and biochemical parasitology

authors： Cabrera Z,Parkhouse RM

更新日期：1986-09-01 00:00:00

abstract：:Trichomonas vaginalis is a parasitic protozoan of both medical and biological relevance. Transcriptional studies in this organism have focused mainly on type II pol promoters, whereas the elements necessary for transcription by polI or polIII have not been investigated. Here, with the aid of a transient transcription ...

journal_title：Molecular and biochemical parasitology

authors： Franco B,Hernández R,López-Villaseñor I

更新日期：2012-09-01 00:00:00

abstract：:The merozoite of Plasmodium vivax possesses a high molecular mass surface protein called Pv-merozoite surface protein 1, PvMSP-1, which exhibits antigenic diversity among isolates. In this study, the extent of sequence variation in the polymorphic region and the flanking interspecies conserved blocks (ICBs) 5 and 6 of...

journal_title：Molecular and biochemical parasitology

authors： Putaporntip C,Jongwutiwes S,Tanabe K,Thaithong S

更新日期：1997-01-01 00:00:00

abstract：:The activity of pyrimethamine and sulfadoxine against two strains of Plasmodium falciparum has been studied in vitro by a radioisotopic technique. Low level antagonism of pyrimethamine resulted from the inclusion of p-aminobenzoic acid, p-aminobenzoylglutamic acid or folic acid in the test medium. Sulfadoxine activity...

journal_title：Molecular and biochemical parasitology

authors： Watkins WM,Sixsmith DG,Chulay JD,Spencer HC

更新日期：1985-01-01 00:00:00

abstract：:Gene expression and its regulation was studied in the rat tapeworm, Hymenolepis diminuta, during strobilization. RNAs extracted from the different developmental stages of the parasite were translated in vitro in a rabbit reticulocyte lysate system. Two-dimensional patterns of the translational products were compared w...

journal_title：Molecular and biochemical parasitology

authors： Siddiqui AA,Karcz SR,Podesta RB

更新日期：1987-08-01 00:00:00

abstract：:Polyacrylamide gel electrophoresis of the total RNA extracted from cultures of Plasmodium falciparum resolved two major RNA components with estimated molecular weights of 1.3 X 10(6) and 0.72 - 0.74 X 10(6). Oligo(dT)-cellulose column chromatography of the total cellular RNA indicates that more than 90% of the total r...

journal_title：Molecular and biochemical parasitology

authors： Vezza AC,Trager W

更新日期：1981-12-01 00:00:00

abstract：:Topoisomerases are enzymes that participate in many cellular functions involving topological manipulation of DNA strands. There are two types of topoisomerases in the cell: (a) type I topoisomerases; and (b) type II topoisomerases (topo II). Previously we have cloned and sequenced the gene encoding Trypanosoma cruzi t...

journal_title：Molecular and biochemical parasitology

authors： Fragoso SP,Mattei D,Hines JC,Ray D,Goldenberg S

更新日期：1998-08-01 00:00:00