Antagonism of sulfadoxine and pyrimethamine antimalarial activity in vitro by p-aminobenzoic acid, p-aminobenzoylglutamic acid and folic acid.

Abstract:

:The activity of pyrimethamine and sulfadoxine against two strains of Plasmodium falciparum has been studied in vitro by a radioisotopic technique. Low level antagonism of pyrimethamine resulted from the inclusion of p-aminobenzoic acid, p-aminobenzoylglutamic acid or folic acid in the test medium. Sulfadoxine activity was antagonised slightly by p-aminobenzoic but not by p-aminobenzoylglutamic acid, and antagonised markedly by folic acid at concentrations above 4 X 10(-8) M. At 10(-7) M folic acid, a concentration lower than that of normal RPMI medium 1640, sulfadoxine activity was reduced 7000 to 9000-fold in comparison with controls. These results are of importance in terms of the utilisation of folates by P. falciparum, the susceptibility of the parasite to antifolate drugs and the in vitro determination of parasite susceptibility.

journal_name

Mol Biochem Parasitol

authors

Watkins WM,Sixsmith DG,Chulay JD,Spencer HC

doi

10.1016/0166-6851(85)90105-7

subject

Has Abstract

pub_date

1985-01-01 00:00:00

pages

55-61

issue

1

eissn

0166-6851

issn

1872-9428

pii

0166-6851(85)90105-7

journal_volume

14

pub_type

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