Molecular species analysis and quantification of the glycosylphosphatidylinositol intermediate glycolipid C from Trypanosoma brucei.

abstract：:The mechanism controlling transcription at several telomeric expression sites for variable surface glycoprotein (VSG) genes in Trypanosoma brucei is unknown. Most VSG genes in expression sites have a region 5' of the gene lacking restriction enzyme sites. This 'barren region' is involved in recombination events which ...

journal_title：Molecular and biochemical parasitology

authors： Shah JS,Young JR,Kimmel BE,Iams KP,Williams RO

更新日期：1987-06-01 00:00:00

abstract：:In silico analyses of Leishmania spp. genome data are a powerful resource to improve the understanding of these pathogens' biology. Trypanosomatids such as Leishmania spp. have their protein-coding genes grouped in long polycistronic units of functionally unrelated genes. The control of gene expression happens by a va...

journal_title：Molecular and biochemical parasitology

authors： Vasconcelos EJ,Terrão MC,Ruiz JC,Vêncio RZ,Cruz AK

更新日期：2012-06-01 00:00:00

abstract：:Profilins are the key regulators of actin dynamics in all eukaryotic cells. However, little information is available on their biochemical properties and functions in kinetoplastids, such as Trypanosoma and Leishmania. We show here that Leishmania parasites express only one homolog of profilin (LdPfn), which catalyzes ...

journal_title：Molecular and biochemical parasitology

authors： Ambaru B,Gopalsamy A,Tammana TVS,Subramanya HS,Gupta CM

更新日期：2020-07-01 00:00:00

abstract：:The apicoplast is a highly specialized organelle that mediates required functions in the growth and replication of apicomplexan parasites. Despite structural conservation of the apicoplast among different parasite genera and species, there are also critical differences in the metabolic requirements of different parasi...

journal_title：Molecular and biochemical parasitology

authors： Caballero MC,Pedroni MJ,Palmer GH,Suarez CE,Davitt C,Lau AO

更新日期：2012-02-01 00:00:00

abstract：:The interaction of Leishmania promastigote surface protease (PSP) with the plasmatic protease inhibitor alpha 2-macroglobulin (alpha 2M) was investigated. In plasma, solubilized PSP forms covalent complexes only with alpha 2M, at the exclusion of other protease inhibitors. The formation of complexes is accompanied by ...

journal_title：Molecular and biochemical parasitology

authors： Heumann D,Burger D,Vischer T,de Colmenares M,Bouvier J,Bordier C

更新日期：1989-02-01 00:00:00

abstract：:Adenylosuccinate synthetase (AdSS) catalyses the Mg(2+) dependent formation of adenylosuccinate from IMP and aspartate, the reaction being driven by the hydrolysis of GTP to GDP. All characterized AdSS thus far exhibit a random kinetic mechanism. We present here kinetic evidence that unlike all other AdSS, Plasmodium ...

journal_title：Molecular and biochemical parasitology

authors： Raman J,Mehrotra S,Anand RP,Balaram H

更新日期：2004-11-01 00:00:00

abstract：:The Plasmodium falciparum Erythrocyte Binding Antigen-175, EBA-175, is a soluble merozoite stage parasite protein which binds to glycophorin A surface receptors on human erythrocytes. We have expressed two conserved cysteine-rich regions, region II and region VI, of this protein as soluble His-tagged polypeptides in i...

journal_title：Molecular and biochemical parasitology

authors： Ockenhouse CF,Barbosa A,Blackall DP,Murphy CI,Kashala O,Dutta S,Lanar DE,Daugherty JR

更新日期：2001-03-01 00:00:00

abstract：:We have identified a GM2-activator protein (GM2AP) with highly unusual properties secreted by the nematode parasite Trichinella spiralis. Expression in Pichia pastoris resulted in a hyperglycosylated protein of 28 kDa, but the 18 kDa native protein was not glycosylated. The parasite GM2AP does not facilitate degradati...

journal_title：Molecular and biochemical parasitology

authors： Bruce AF,Gares MP,Selkirk ME,Gounaris K

更新日期：2006-06-01 00:00:00

abstract：:The capability of synthesizing fatty acids de novo in the meront stage of the oyster protozoan parasite, Perkinsus marinus, was investigated employing stable-isotope-labeled precursors (1,2 13C-acetate and palmitic-d(31) acid). Fatty acid methyl esters derived from 1,2 13C-acetate and palmitic-d(31) acid were analyzed...

journal_title：Molecular and biochemical parasitology

authors： Chu FL,Lund E,Soudant P,Harvey E

更新日期：2002-02-01 00:00:00

abstract：:With the rapid spread of drug-resistant strains of Plasmodium falciparum, the development of new antimalarials is an urgent need. As malaria parasites live in a highly pro-oxidant environment, their anti-oxidant defences have frequently been suggested as candidate drug targets. A key point in such defences is the prod...

journal_title：Molecular and biochemical parasitology

authors： Aparicio IM,Marín-Menéndez A,Bell A,Engel PC

更新日期：2010-08-01 00:00:00

abstract：:Messenger RNA has been isolated from adults and eggs of Schistosoma mansoni and translated in vitro in mRNA dependent rabbit reticulocyte lysate. The in vitro translation products have been immunoprecipitated using a wide variety of hyperimmune and infection sera from rabbits, mice and humans. A large number of in vit...

journal_title：Molecular and biochemical parasitology

authors： Taylor DW,Cordingley JS,Butterworth AE

更新日期：1984-03-01 00:00:00

abstract：:Phosphoserine phosphatase (PSP) catalyzes the third step of the phosphorylated serine biosynthetic pathway, and occurred multiple times in evolution, while enzymes catalyzing the first and second steps in the pathway have single respective origins. In the present study, we examined the existence of PSP among genus Ent...

journal_title：Molecular and biochemical parasitology

authors： Chiba Y,Makiuchi T,Jeelani G,Nozaki T

更新日期：2016-06-01 00:00:00

abstract：:Malaria parasites possess a de novo heme synthetic pathway. Interestingly, this pathway is dispensable during the blood stages of development in mammalian hosts. The assembly of the two most important hemeproteins, cytochromes c and c1, is mediated by cytochrome heme lyase enzymes. Plasmodium spp. possess two cytochro...

journal_title：Molecular and biochemical parasitology

authors： Posayapisit N,Songsungthong W,Koonyosying P,Falade MO,Uthaipibull C,Yuthavong Y,Shaw PJ,Kamchonwongpaisan S

更新日期：2016-01-01 00:00:00

abstract：:A poly-zinc finger protein, designated PZFP1 was identified in Trypanosoma cruzi for the first time. The protein has 191 amino acids, contains seven motifs Cys(X)(2)Cys(X)(4)His(X)(4)Cys. A recombinant PZFP1 was generated in E. coli and the expected 21kDa polypeptide co-purified with two other inducible products of ab...

journal_title：Molecular and biochemical parasitology

authors： Espinosa JM,Portal D,Lobo GS,Pereira CA,Alonso GD,Gómez EB,Lan GH,Pomar RV,Flawiá MM,Torres HN

更新日期：2003-09-01 00:00:00

abstract：:Precipitin arcs formed after immunoelectrophoresis of Schistosoma mansoni egg, worm and cercarial antigens with polyspecific rabbit antisera have been 'stained' at neutral pH with chromogenic enzyme substrates specific for proteases and peptidases. A total of 7 antigenically distinct enzymes with peptidolytic properti...

journal_title：Molecular and biochemical parasitology

authors： Doenhoff MJ,Modha J,Curtis RH,Adeoye GO

更新日期：1988-12-01 00:00:00

abstract：:Glutathione S-transferases (GSTs) constitute a major detoxification mechanism in helminth organisms and are regarded vaccine candidates against helminth infections. Onchocerca volvulus glutathione-binding proteins were purified from the aqueous soluble fraction of homogenised adult females by affinity chromatography o...

journal_title：Molecular and biochemical parasitology

authors： Salinas G,Braun G,Taylor DW

更新日期：1994-07-01 00:00:00

abstract：:Among several protease inhibitors tested, only leupeptin was found to modify qualitatively the processing of P126, a major antigen of the parasitophorous vacuole of Plasmodium falciparum, and to inhibit the release of merozoites. Whereas P126 is normally processed upon merozoite release into 2 polypeptides of 50 and 7...

journal_title：Molecular and biochemical parasitology

authors： Debrabant A,Delplace P

更新日期：1989-03-01 00:00:00

abstract：:Survival of Onchocerca volvulus, a pathogenic human filarial parasite, is likely to depend upon the detoxification activities of the glutathione S-transferases (GSTs). The 24 kDa O. volvulus GST, OvGST2, was expressed in a bacterial system and the recombinant protein was purified to homogeneity by affinity chromatogra...

journal_title：Molecular and biochemical parasitology

authors： Liebau E,Wildenburg G,Brophy PM,Walter RD,Henkle-Dührsen K

更新日期：1996-09-01 00:00:00

abstract：:This study on isolated cercarial bodies demonstrates that the biological transformation from cercaria to schistosomulum and the biochemical transition from an aerobic to an anaerobic energy metabolism are separate processes, which are not necessarily linked. The metabolic transition depends on the external glucose con...

journal_title：Molecular and biochemical parasitology

authors： Horemans AM,Tielens AG,van den Bergh SG

更新日期：1992-03-01 00:00:00

abstract：:In an effort to eradicate malaria, new interventions are proposed to include compound/vaccine development against pre-erythrocytic, erythrocytic and mosquito stages of Plasmodium. Drug repurposing might be an alternative approach to new antimalarials reducing the cost and the time required for drug development. Previo...

journal_title：Molecular and biochemical parasitology

authors： Goulielmaki E,Kaforou S,Venugopal K,Loukeris TG,Siden-Kiamos I,Koussis K

更新日期：2018-03-01 00:00:00

abstract：:The Leishmania GP63 major surface protein gene family encodes multiple isoforms which differ predominantly in the carboxyterminal region. We have isolated 4 full-length gp63 cDNA clones derived from stationary-phase promastigote RNA of a cloned isolate of Leishmania guyanensis, a member of the braziliensis complex. Th...

journal_title：Molecular and biochemical parasitology

authors： Steinkraus HB,Greer JM,Stephenson DC,Langer PJ

更新日期：1993-12-01 00:00:00

abstract：:Surface labeling studies using two populations of Onchocerca gibsoni microfilariae revealed important differences in major radioiodinated proteins. Small numbers of microfilariae harvested from the skin of cattle or the uteri of adult worms from skin nodules were purified, radioiodinated, solubilized and the proteins ...

journal_title：Molecular and biochemical parasitology

authors： Forsyth KP,Copeman DB,Mitchell GF

更新日期：1984-02-01 00:00:00

abstract：:Trypanosoma brucei harbors a unique multifunctional RNA polymerase (pol) I which transcribes, in addition to ribosomal RNA genes, the gene units encoding the major cell surface antigens variant surface glycoprotein and procyclin. In consequence, this RNA pol I is recruited to three structurally different types of prom...

journal_title：Molecular and biochemical parasitology

authors： Nguyen TN,Schimanski B,Zahn A,Klumpp B,Günzl A

更新日期：2006-09-01 00:00:00

abstract：:Subtilisin-like proteases of malaria parasite Plasmodium falciparum (PfSUB1, 2 and 3) are expressed at late asexual blood stages. PfSUB1 and 2 are considered important drug targets due to their essentiality for parasite blood stages and role in merozoite egress and invasion of erythrocytes. We have earlier shown the i...

journal_title：Molecular and biochemical parasitology

authors： Alam A,Bhatnagar RK,Relan U,Mukherjee P,Chauhan VS

更新日期：2013-10-01 00:00:00

abstract：:Malarial merozoites use an array of ligands, including members of the Reticulocyte Binding Like (RBL) super-family of invasion proteins, to identify and invade erythrocytes. RBL family members are large Type I membrane anchored proteins expressed at the invasive end of merozoites that share homology with the Reticuloc...

journal_title：Molecular and biochemical parasitology

authors： Rayner JC,Huber CS,Galinski MR,Barnwell JW

更新日期：2004-02-01 00:00:00

abstract：:Tubulin-binding cofactor C stimulates GTPase activity and contributes to the release of the heterodimeric α/β-tubulin from a super-complex of tubulin monomers and two ancillary cofactors. We have determined the 2.2 Å resolution crystal structure of the C-terminal domain of tubulin-binding cofactor C from Leishmania ma...

journal_title：Molecular and biochemical parasitology

authors： Barrack KL,Fyfe PK,Finney AJ,Hunter WN

更新日期：2015-05-01 00:00:00

abstract：:Nucleoside analogs are potential anti-Leishmania agents. To better understand how these compounds might lose their effectiveness, Leishmania were independently selected for resistance to inosine dialdehyde or tubercidin. Each of the resistant cells exhibited resistance to inosine dialdehyde and tubercidin as well as t...

journal_title：Molecular and biochemical parasitology

authors： Kerby BR,Detke S

更新日期：1993-08-01 00:00:00

abstract：:The protozoan parasite Trypanosoma brucei relies on trans-splicing to process its mRNAs. A novel nuclear serine/arginine (SR)-rich trypanosomal protein (TSR1) was characterized which contains two RNA recognition motifs. The TSR1 protein appears to be homologous to RNA-binding SR proteins of the cis-splicing machinery ...

journal_title：Molecular and biochemical parasitology

authors： Ismaïli N,Pérez-Morga D,Walsh P,Mayeda A,Pays A,Tebabi P,Krainer AR,Pays E

更新日期：1999-07-30 00:00:00

abstract：:We have isolated and characterized a gene encoding a novel GTP-binding protein of the GTPase superfamily in the filarial parasites Brugia malayi and Onchocerca volvulus. The deduced amino acid sequence of the cloned molecule has approximately 30% overall homology to ras proteins and approximately 90% homology to the '...

journal_title：Molecular and biochemical parasitology

authors： Dissanayake S,Xu M,Piessens WF

更新日期：1992-12-01 00:00:00

abstract：:The membrane potential and pH gradient over the plasma membrane of the protozoan parasite Trypanosoma brucei were measured with radioactive indicators in combination with the silicone oil centrifugation technique over a range of temperatures. At 37 degrees C a small membrane potential and pH gradient of similar magnit...

journal_title：Molecular and biochemical parasitology

authors： Ter Kuile BH,Wiemer EA,Michels PA,Opperdoes FR

更新日期：1992-10-01 00:00:00