Abstract:
:Protozoan parasites of the genus Leishmania have a digenetic lifecycle, alternating between the promastigote and amastigote stages. The extracellular promastigote resides within a sandfly vector, while the obligate intracellular amastigote stage replicates in the phagolysosome of mammalian host macrophages. Adaptation to and survival within these vastly differently environments is accompanied by differential expression of a subset of genes, which is regulated post-transcriptionally via cis-acting elements in 3' untranslated region (3'UTR) or intercistronic sequences. It was reported previously that Leishmania mexicana A600-4 mRNA transcript abundance was eight-fold higher in the amastigotes. In this study, chimeric luciferase:A600-4 3'UTR reporter constructs were integrated at the A600 chromosome locus to identify regulatory regions of the A600-4 3'UTR sequence. Evidence is provided for distinct 3'UTR elements that function to stabilize the A600-4 mRNA transcript in the amastigote stage and to regulate translation efficiency, respectively.
journal_name
Mol Biochem Parasitoljournal_title
Molecular and biochemical parasitologyauthors
Murray A,Fu C,Habibi G,McMaster WRdoi
10.1016/j.molbiopara.2007.02.010subject
Has Abstractpub_date
2007-06-01 00:00:00pages
125-32issue
2eissn
0166-6851issn
1872-9428pii
S0166-6851(07)00067-9journal_volume
153pub_type
杂志文章abstract::A 230 000 molecular weight (MW) Plasmodium yoelii protein, a 250 000 MW P. chabaudi protein and a 195 000 MW P. falciparum protein, identified using monoclonal antibodies, have similar characteristics, and have been implicated as protective antigens. In this study the serological relationship between these proteins wa...
journal_title:Molecular and biochemical parasitology
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章,评审
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abstract::The cyst wall of Entamoeba invadens (Ei), a model for the human pathogen Entamoeba histolytica, contains chitin, which is a homopolymer of beta-1, 4-linked N-acetyl-glucosamine (GlcNAc). In fungi and in bacteria that make nodulation factors, chitin deacetylases make chitosan, which is a mixture of GlcNAc and glucosami...
journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章
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更新日期:2006-07-01 00:00:00
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章
doi:10.1016/s0166-6851(96)02765-x
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章
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journal_title:Molecular and biochemical parasitology
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章
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更新日期:2015-05-01 00:00:00
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章
doi:10.1016/0166-6851(92)90202-u
更新日期:1992-03-01 00:00:00
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章
doi:10.1016/s0166-6851(97)00152-7
更新日期:1997-12-01 00:00:00
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journal_title:Molecular and biochemical parasitology
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doi:10.1016/j.molbiopara.2013.07.003
更新日期:2013-09-01 00:00:00
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章
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更新日期:1992-08-01 00:00:00
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章
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更新日期:1997-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0166-6851(95)02474-3
更新日期:1995-10-01 00:00:00
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章
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更新日期:2015-08-01 00:00:00
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章
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更新日期:1984-04-01 00:00:00
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journal_title:Molecular and biochemical parasitology
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章
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更新日期:1992-01-01 00:00:00
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章
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更新日期:2011-05-01 00:00:00
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章
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