当前位置： SCI文献检索 > MOLECULAR AND BIOCHEMICAL PARASITOLOGY期刊下所有文献 > Cloning and sequence analysis of the Schistosoma mansoni membrane glycoprotein antigen gene GP22.

Cloning and sequence analysis of the Schistosoma mansoni membrane glycoprotein antigen gene GP22.

Abstract：

:A family of Schistosoma mansoni proteins (18-22 kDa, pI 5.3-5.8) are biosynthesized in juvenile worms and immunoprecipitated by antibodies uniquely present in protective Fischer rat antiserum. A cDNA clone, lambda gt11-40, expressing epitopes common to this protein family was used to obtain a genomic DNA clone, by hybridization with a lambda gt11-40 oligonucleotide probe. In the 1.37 kb of genomic DNA sequenced, an open reading frame of 182 amino acids was identified on the strand corresponding to lambda gt11-40 coding sequences, and those of identical independently isolated cDNA clones defining a 25-kDa surface membrane glycoprotein. The new S. mansoni gene is termed GP22. There are two candidate promoters, confirmed by primer extension studies with worm RNA. Promoter 1 (P1) is preceded by a G + C-rich region and potential CAAT sequences, and is to the 5'-side of P2. Transcription from P1 is initiated at 2 different sites, apparently producing mRNAs with different translation start sites (ATG). Decoding these mRNAs yields protein products of 182 (P1), 175 (P1), 140 (P2) and 136 (P2) amino acids. The polypeptides share the following features: a hydrophobic segment near the carboxy terminus sufficient to span a lipid bilayer, with a consensus sequence for thio-esterification by a fatty acid; an external domain containing 2 potential N-linked glycosylation sites; and a candidate leucine-zipper motif, suggesting the protein may exist as a dimer on the worm surface. While sharing these common features in their carboxy terminal regions, the three proteins differ in the length and properties of their amino termini. The 140-amino acid protein has a short hydrophobic amino terminus, while the 175- and 182-amino acid proteins have more extensive hydrophobic sequences, each preceded by a hydrophilic amino terminal sequence. The heterogeneity observed in 2-dimensional gels of the antigen may be explained in part by the size and charge differences among the proteins deduced from the sequence and transcription pattern of this gene. The possibility of stage-specific regulated expression of this candidate vaccine antigen family is an attractive concept, potentially accounting for the phenomenon of concomitant immunity observed in the rat and perhaps other schistosome hosts.

journal_name

Mol Biochem Parasitol

journal_title

Molecular and biochemical parasitology

authors

el-Sherbeini M,Ramadan N,Bostian KA,Knopf PM

doi

10.1016/0166-6851(91)90132-p

subject

Has Abstract

pub_date

1991-11-01 00:00:00

pages

83-98

issue

1

eissn

0166-6851

issn

1872-9428

pii

0166-6851(91)90132-P

journal_volume

49

pub_type

杂志文章

相关文献

MOLECULAR AND BIOCHEMICAL PARASITOLOGY文献大全
  • Characterization of a Giardia lamblia variant-specific surface protein (VSP) gene from isolate GS/M and estimation of the VSP gene repertoire size.

    abstract：:Giardia lamblia undergoes surface antigenic variation. The variant-specific surface proteins (VSPs) of isolate WB are cysteine-rich, can vary dramatically in size, contain Cys-X-X-Cys motifs, and are differentially expressed. GS/M(H7) is a Giardia clone from a different isolate which expresses a VSP epitope not found ...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(92)90072-r

    authors： Nash TE,Mowatt MR

    更新日期：1992-04-01 00:00:00

  • Characterization and localization of Plasmodium falciparum homolog of prokaryotic ClpQ/HslV protease.

    abstract：:The beta subunits (beta1, beta2, and beta5) of 20S proteasome and HslV/ClpQ are ATP-dependent threonine proteases present in eukaryotes and prokaryotes, respectively that control levels of key regulatory proteins in the cell. The orthologue of prokaryotic HslV protease in Plasmodium falciparum (PfHslV) is a novel drug...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2007.01.002

    authors： Ramasamy G,Gupta D,Mohmmed A,Chauhan VS

    更新日期：2007-04-01 00:00:00

  • Nuclear factors binding to the mini-exon repeat of Trypanosoma brucei.

    abstract：:In trypanosomes and related kinetoplastida a common short 5' leader sequence is encoded by the highly repetitive mini-exon gene and added to all protein coding mRNAs by trans-splicing. To initiate a search for transcription factors associated with transcription of the mini-exon we have used an electrophoretic mobility...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(88)90136-3

    authors： Koronakis VE,Ross AM,Le Page RW,Hughes C

    更新日期：1988-07-01 00:00:00

  • The influence of temperature and serum deprivation on the synthesis of heat-shock proteins and alpha and beta tubulin in promastigotes of Leishmania major.

    abstract：:We have examined changes in the relative synthesis of individual proteins in promastigotes of Leishmania major subjected to decreasing serum levels in vitro. We observed increases in the relative synthesis of the putative heat-shock proteins of 82 and 70 kDa and of proteins of 79 and 41 kDa but decreases in the synthe...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(89)90136-9

    authors： Toye P,Remold H

    更新日期：1989-06-01 00:00:00

  • Identification by extrachromosomal amplification and overexpression of a zeta-crystallin/NADPH-oxidoreductase homologue constitutively expressed in Leishmania spp.

    abstract：:A gene which overexpresses a 36-kDa protein (p36) in tunicamycin-resistant Leishmania was mapped by transfection and overexpression to the upstream region of the drug maker in the extrachromosomal amplicon. Complete sequencing of this region revealed a single open reading frame of about 1 kb. Authenticity of the clone...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(94)90147-3

    authors： Liu X,Chang KP

    更新日期：1994-08-01 00:00:00

  • Characterization of protein kinase CK2 from Trypanosoma brucei.

    abstract：:CK2 is a ubiquitous but enigmatic kinase. The difficulty in assigning a role to CK2 centers on the fact that, to date, no biologically relevant modulator of its function has been identified. One common theme revolves around a constellation of known substrates involved in growth control, compatible with its concentrati...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2006.10.002

    authors： Jensen BC,Kifer CT,Brekken DL,Randall AC,Wang Q,Drees BL,Parsons M

    更新日期：2007-01-01 00:00:00

  • Molecular characterisation and localisation of an Onchocerca volvulus pi-class glutathione S-transferase.

    abstract：:Glutathione S-transferases (GSTs) constitute a major detoxification mechanism in helminth organisms and are regarded vaccine candidates against helminth infections. Onchocerca volvulus glutathione-binding proteins were purified from the aqueous soluble fraction of homogenised adult females by affinity chromatography o...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(94)90030-2

    authors： Salinas G,Braun G,Taylor DW

    更新日期：1994-07-01 00:00:00

  • Heterogeneity of the serine synthetic pathway in Entamoeba species.

    abstract：:Phosphoserine phosphatase (PSP) catalyzes the third step of the phosphorylated serine biosynthetic pathway, and occurred multiple times in evolution, while enzymes catalyzing the first and second steps in the pathway have single respective origins. In the present study, we examined the existence of PSP among genus Ent...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2016.06.002

    authors： Chiba Y,Makiuchi T,Jeelani G,Nozaki T

    更新日期：2016-06-01 00:00:00

  • Upstream regulatory elements controlling expression of the Entamoeba histolytica lectin.

    abstract：:Entamoeba histolytica genomic organization and putative promoter elements appear to be distinct from both metazoan and better characterized protozoan organisms. The recent development of DNA-mediated transfection for E. histolytica enabled characterization of cis-acting promoter elements required for gene expression. ...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/s0166-6851(96)02614-x

    authors： Purdy JE,Pho LT,Mann BJ,Petri WA Jr

    更新日期：1996-06-01 00:00:00

  • Isolation and functional characterization of snail hemocyte-modulating polypeptide from primary sporocysts of Schistosoma mansoni.

    abstract：:Infection with larval trematodes has been shown to inhibit several snail-host defences, including hemocyte phagocytosis, cytotoxicity, motility, and adherence. Certain plasma factors which mediate snail defence responses, and which may be produced by host hemocytes, also appear to be altered by these parasites. In thi...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(91)90124-o

    authors： Lodes MJ,Connors VA,Yoshino TP

    更新日期：1991-11-01 00:00:00

  • Antigenic variation and cytoadhesion in Babesia bovis and Plasmodium falciparum: different logics achieve the same goal.

    abstract：:Babesia bovis is a protozoal hemoparasite of cattle which behaves in certain crucial respects like Plasmodium falciparum, despite being phylogenetically distant and having many differences in its life cycle. The shared behavioral attributes of rapid antigenic variation and cytoadhesion/sequestration are thought to con...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章,评审

    doi：10.1016/j.molbiopara.2003.09.012

    authors： Allred DR,Al-Khedery B

    更新日期：2004-03-01 00:00:00

  • Actin sequestering protein, profilin, regulates intracellular vesicle transport in Leishmania.

    abstract：:Profilins are the key regulators of actin dynamics in all eukaryotic cells. However, little information is available on their biochemical properties and functions in kinetoplastids, such as Trypanosoma and Leishmania. We show here that Leishmania parasites express only one homolog of profilin (LdPfn), which catalyzes ...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2020.111280

    authors： Ambaru B,Gopalsamy A,Tammana TVS,Subramanya HS,Gupta CM

    更新日期：2020-07-01 00:00:00

  • Cloning and characterization of the ribosomal RNA gene repeat from Ostertagia ostertagi.

    abstract：:Clones of the rRNA genes of Ostertagia ostertagi were selected from a library prepared from the genomic DNA of adult worms of strain LA-2. A 13.4-kb insert in a clone, lambda OOR78, is comprised of one complete 7.5-kb rDNA unit and portions of two adjacent units. The rDNA unit is directly repeated in a head-to-tail fa...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(91)90095-n

    authors： Dame JB,Yowell CA,Courtney CH,Lindgren WG

    更新日期：1991-04-01 00:00:00

  • Peptide-fluoromethyl ketones arrest intracellular replication and intercellular transmission of Trypanosoma cruzi.

    abstract：:The major proteolytic activity of Trypanosoma cruzi is a cathepsin L-like cysteine protease expressed in all stages of the parasite. As an initial step in identifying possible functions of this enzyme in the life cycle of T. cruzi, and examining its potential as a target for rational drug design, two fluoromethyl keto...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(93)90086-d

    authors： Harth G,Andrews N,Mills AA,Engel JC,Smith R,McKerrow JH

    更新日期：1993-03-01 00:00:00

  • Effects of curcumin on the parasite Schistosoma mansoni: a transcriptomic approach.

    abstract：:Schistosomiasis remains a severe problem of public health in developing countries. Several reports show that praziquantel, the drug of choice for treating schistosomiasis, can select Schistosoma mansoni strains resistant to the drug. Thus, developing new drugs against this parasitosis is a highly desirable goal. Curcu...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2012.11.006

    authors： Morais ER,Oliveira KC,Magalhães LG,Moreira EB,Verjovski-Almeida S,Rodrigues V

    更新日期：2013-02-01 00:00:00

  • Tests of cytoplasmic RNA interference (RNAi) and construction of a tetracycline-inducible T7 promoter system in Trypanosoma cruzi.

    abstract：:The technique of RNA interference (RNAi) is exceedingly useful for knocking down the expression of a specific mRNA in African trypanosomes and other organisms for the purpose of examining the function of its gene. However, when we attempted to apply RNAi in the Latin American trypanosome, Trypanosoma cruzi, to diminis...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2003.10.005

    authors： DaRocha WD,Otsu K,Teixeira SM,Donelson JE

    更新日期：2004-02-01 00:00:00

  • Sequence heterogeneity and polymorphic gene arrangements of the Leishmania guyanensis gp63 genes.

    abstract：:The Leishmania GP63 major surface protein gene family encodes multiple isoforms which differ predominantly in the carboxyterminal region. We have isolated 4 full-length gp63 cDNA clones derived from stationary-phase promastigote RNA of a cloned isolate of Leishmania guyanensis, a member of the braziliensis complex. Th...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(93)90107-9

    authors： Steinkraus HB,Greer JM,Stephenson DC,Langer PJ

    更新日期：1993-12-01 00:00:00

  • Molecular and functional characterisation of the heat shock protein 10 of Strongyloides ratti.

    abstract：:Strongyloides stercoralis and S. ratti are intestinal parasitic nematodes infecting rats and humans, respectively. Both present extraordinary life cycles comprising a free-living generation in addition to parasitic stages. In search of molecules possibly involved in parasite-host interaction, we performed mass spectro...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2009.07.007

    authors： Tazir Y,Steisslinger V,Soblik H,Younis AE,Beckmann S,Grevelding CG,Steen H,Brattig NW,Erttmann KD

    更新日期：2009-12-01 00:00:00

  • Molecular cloning of GRA4, a Toxoplasma gondii dense granule protein, recognized by mucosal IgA antibodies.

    abstract：:Clones which were selected from a Toxoplasma gondii expression library with the immune serum from a T. gondii-infected rabbit, were further screened using milk and intestinal secretions from mice which had been orally infected with T. gondii cysts. The gene products of several clones reacted strongly with milk IgA and...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(92)90172-g

    authors： Mevelec MN,Chardès T,Mercereau-Puijalon O,Bourguin I,Achbarou A,Dubremetz JF,Bout D

    更新日期：1992-12-01 00:00:00

  • Molecular cloning of Trypanosoma brucei CK2 catalytic subunits: the alpha isoform is nucleolar and phosphorylates the nucleolar protein Nopp44/46.

    abstract：:We have demonstrated previously that Nopp44/46, an abundant nucleolar phosphoprotein of Trypanosoma brucei, is associated with a protein kinase. In many organisms multiple nucleolar proteins are phosphorylated by the protein kinase CK2, formerly known as casein kinase II. Here we report the identification of two T. br...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/s0166-6851(01)00407-8

    authors： Park JH,Brekken DL,Randall AC,Parsons M

    更新日期：2002-01-01 00:00:00

  • Extensive homologies between Leishmania donovani chromosomes of markedly different size.

    abstract：:The smallest chromosome (230 kb) of the HU3 stock of Leishmania donovani was purified from an orthogonal field alternation (OFAGE) gel, digested with PstI and cloned into the plasmid pUC13. When used to probe Southern blots of OFAGE gels, the cloned sequences recognised one or more large chromosomes in all L. donovani...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(90)90198-u

    authors： Bishop RP

    更新日期：1990-01-01 00:00:00

  • Identification of trypanosomatid PEX19: functional characterization reveals impact on cell growth and glycosome size and number.

    abstract：:Glycosomes are peroxisome-like organelles present in trypanosomatid pathogens. These organelles compartmentalize glycolysis, among other reactions, and are essential in both bloodstream and procyclic form Trypanosoma brucei. Peroxins (PEXs) are proteins necessary for biogenesis of peroxisomes and glycosomes. In each a...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2005.03.008

    authors： Banerjee SK,Kessler PS,Saveria T,Parsons M

    更新日期：2005-07-01 00:00:00

  • Substrate specificity of recombinant cysteine proteinase, CPB, of Leishmania mexicana.

    abstract：:The primary S(1) subsite specificity of a recombinant cysteine proteinase, CPB2.8 Delta CTE, of Leishmania mexicana was investigated in a systematic way using a series of peptides derived from Abz-KLRFSKQ-EDDnp in which Arg was substituted by all natural amino acids (where Abz is ortho-amino-benzoyl and EDDnp is N-[2,...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/s0166-6851(01)00290-0

    authors： Alves LC,Judice WA,St Hilaire PM,Meldal M,Sanderson SJ,Mottram JC,Coombs GH,Juliano L,Juliano MA

    更新日期：2001-08-01 00:00:00

  • A schizont-derived protein, TpSCOP, is involved in the activation of NF-kappaB in Theileria parva-infected lymphocytes.

    abstract：:Theileria parva is a tick-transmitted intracellular protozoan parasite that causes East Coast fever, a fatal bovine lymphoproliferative disease. The molecular mechanisms that underlie host cell transformation by T. parva schizonts have been studied extensively, and it is known that the nuclear factor-kappa B (NF-kappa...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2010.06.005

    authors： Hayashida K,Hattori M,Nakao R,Tanaka Y,Kim JY,Inoue N,Nene V,Sugimoto C

    更新日期：2010-11-01 00:00:00

  • Role of porcine serum haptoglobin in the host-parasite relationship of Taenia solium cysticercosis.

    abstract：:Human and porcine cysticercosis is a parasitic disease caused by the larval stage (cysts) of the tapeworm Taenia solium. Cysts may live in several host tissues such as skeletal muscle or brain. We have previously described the presence of host haptoglobin (Hp) and hemoglobin (Hb) in different protein extracts of the T...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2016.05.010

    authors： Navarrete-Perea J,Toledano-Magaña Y,De la Torre P,Sciutto E,Bobes RJ,Soberón X,Laclette JP

    更新日期：2016-06-01 00:00:00

  • Molecular cloning and partial characterization of ribosomal RNA genes from Trypanosoma cruzi.

    abstract：:To further analyze the organization of the nuclear rDNA locus in Trypanosoma cruzi, genomic recombinant plasmid clones were constructed and isolated after hybridization with rRNA molecules as hybridization probes. Approximately 11 kilobase pairs from the cistron were cloned in three recombinant plasmids carrying adjac...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(88)90047-3

    authors： Hernández R,Díaz-de Léon F,Castañeda M

    更新日期：1988-01-15 00:00:00

  • Surface-associated antigens of Brugia malayi L2 and L3 parasites during vector-stage development.

    abstract：:Surface and metabolic labeling procedures were used to characterize the composition and the time of expression of Brugia malayi L2 and L3 surface-associated molecules as the larvae develop within the mosquito vector. Larvae were harvested from mosquito tissues at 5 (early L2), 8 (late L2) and 11 (L3) days post-infecti...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(92)90039-m

    authors： Ibrahim MS,Richie TL,Scott AL

    更新日期：1992-05-01 00:00:00

  • Long-range restriction maps of size-variable homologous chromosomes in Leishmania infantum.

    abstract：:In order to clarify the interpretation of molecular karyotype polymorphisms in Leishmania, the three smallest chromosomes from five cloned strains of Leishmania infantum were identified by chromosome-specific anonymous DNA probes. The presence in the same clone of homologous chromosomes of a different size was demonst...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(91)90053-9

    authors： Blaineau C,Bastien P,Rioux JA,Roizès G,Pagès M

    更新日期：1991-06-01 00:00:00

  • Identification of antigens of Onchocerca volvulus and Onchocerca gibsoni for diagnostic use.

    abstract：:Adults of Onchocerca volvulus and Onchocerca gibsoni were identically fractionated into a surface-enriched fraction, a phosphate buffered saline (PBS) extract and a PBS insoluble-detergent soluble fraction. Glycoproteins were prepared from these extracts and all fractions were examined by the Western blot technique us...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(86)90102-7

    authors： Cabrera Z,Parkhouse RM

    更新日期：1986-09-01 00:00:00

  • Multimerization of the Toxoplasma gondii MIC2 integrin-like A-domain is required for binding to heparin and human cells.

    abstract：:Toxoplasma gondii is an obligate intracellular parasite that causes toxoplasmosis in humans and animals. To invade host cells, T. gondii deploys the contents of its apically oriented secretory organelles that include the micronemes. Contained within the micronemes are proteins that possess adhesive motifs resembling t...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2003.12.001

    authors： Harper JM,Hoff EF,Carruthers VB

    更新日期：2004-04-01 00:00:00