Energy metabolism in Cotugnia digonopora and the effect of anthelmintics.

abstract：:Blood-stage malaria vaccine candidates include surface proteins of the merozoite. Antibodies to these proteins may either block essential steps during invasion or render the merozoite susceptible to phagocytosis or complement-mediated degradation. Structural information on merozoite surface proteins complexed to antib...

journal_title：Molecular and biochemical parasitology

authors： Dekker C,Uthaipibull C,Calder LJ,Lock M,Grainger M,Morgan WD,Dodson GG,Holder AA

更新日期：2004-09-01 00:00:00

abstract：:We have assessed the phylogenetic status of the Trypanosoma cruzi Genome Project CL Brener reference strain by multilocus enzyme electrophoresis (MLEE) and multiprimer random amplified polymorphic DNA (RAPD) including a set of cloned stocks representative of the whole genetic diversity of T. cruzi. MLEE and RAPD data ...

journal_title：Molecular and biochemical parasitology

authors： Brisse S,Barnabé C,Bañuls AL,Sidibé I,Noël S,Tibayrenc M

更新日期：1998-05-01 00:00:00

abstract：:Antibodies raised against a Leishmania major recombinant promastigote surface antigen 2 (PSA-2) fragment recognized three major polypeptides of approximate M(r) 96,000, 80,000 and 50,000 in promastigotes of three Israeli isolates of L. major including the cloned line LRC-L137-V121, but detected a different array of po...

journal_title：Molecular and biochemical parasitology

authors： Symons FM,Murray PJ,Ji H,Simpson RJ,Osborn AH,Cappai R,Handman E

更新日期：1994-09-01 00:00:00

abstract：:Three days after mice were inoculated with Trypanosoma brucei gambiense, total hepatic cytochrome P-450 levels were decreased 14% from control values, while the specific mixed-function oxidase activity (per nmole of cytochrome P-450) was inhibited almost 40% in infected animals. Furthermore, drugs used to treat trypan...

journal_title：Molecular and biochemical parasitology

authors： Shertzer HG,Hall JE,Seed JR

更新日期：1981-08-01 00:00:00

abstract：:Thrombospondin-related anonymous protein (TRAP) family members participate in attachment and invasion of host cells by apicomplexan parasites. A TRAP homologue in Neospora caninum strain Nc-1 (NcMIC2) was cloned, sequenced and found to be 61% identical (75% similar) at the amino acid level to Toxoplasma gondii MIC2 (T...

journal_title：Molecular and biochemical parasitology

authors： Lovett JL,Howe DK,Sibley LD

更新日期：2000-03-15 00:00:00

abstract：:The capability of synthesizing fatty acids de novo in the meront stage of the oyster protozoan parasite, Perkinsus marinus, was investigated employing stable-isotope-labeled precursors (1,2 13C-acetate and palmitic-d(31) acid). Fatty acid methyl esters derived from 1,2 13C-acetate and palmitic-d(31) acid were analyzed...

journal_title：Molecular and biochemical parasitology

authors： Chu FL,Lund E,Soudant P,Harvey E

更新日期：2002-02-01 00:00:00

abstract：:Erythrocytes from Plasmodium berghei-infected mice on incubation either in plasma or artificial isotonic media showed an increase in uptake of 45Ca2+ compared with erythrocytes from uninfected mice. Infected cells (55% parasitaemia) incubated in plasma from normal or infected mice gave uptake rates of 9.8 and 8.1 nmol...

journal_title：Molecular and biochemical parasitology

authors： Krungkrai J,Yuthavong Y

更新日期：1983-03-01 00:00:00

abstract：:Schistosoma mansoni schistosomula cultured in the presence of phospholipids showed a decreased sensitivity to the lethal complement-mediated action of anti-schistosome antibodies. Phosphatidyl choline, sphingomyelin and phosphatidyl ethanolamine had a protective action on the schistosomula transformed in vitro by pass...

journal_title：Molecular and biochemical parasitology

authors： Billecocq A

更新日期：1987-09-01 00:00:00

abstract：:Opisthorchis viverrini is the causative agent of human opisthorchiasis in Thailand and long lasting infection with the parasite has been correlated with the development of cholangiocarcinoma. In this work we have molecularly characterized the first member of a protein family carrying two DM9 repeats in this parasite (...

journal_title：Molecular and biochemical parasitology

authors： Labbunruang N,Phadungsil W,Tesana S,Smooker PM,Grams R

更新日期：2016-05-01 00:00:00

abstract：:We have examined changes in the relative synthesis of individual proteins in promastigotes of Leishmania major subjected to decreasing serum levels in vitro. We observed increases in the relative synthesis of the putative heat-shock proteins of 82 and 70 kDa and of proteins of 79 and 41 kDa but decreases in the synthe...

journal_title：Molecular and biochemical parasitology

authors： Toye P,Remold H

更新日期：1989-06-01 00:00:00

abstract：:Many of the currently available nematicides used in nematode control are hazardous to the user, environment and beneficial non-target organisms. Therefore the need to develop alternative methods for nematode control such as the development of nematode-resistant crops through RNA-mediated interference (RNAi) holds grea...

journal_title：Molecular and biochemical parasitology

authors： Joseph S,Gheysen G,Subramaniam K

更新日期：2012-11-01 00:00:00

abstract：:The presence of virus-derived RNA was investigated in 38 axenically growing Giardia isolates from different geographic areas. The RNA virus was demonstrated in Giardia strains from humans in the U.S.A., England and the majority of strains from Poland. Two strains isolated respectively from a cat and a cavia also conta...

journal_title：Molecular and biochemical parasitology

authors： De Jonckheere JF,Gordts B

更新日期：1987-02-01 00:00:00

abstract：:During the replicative cycle of many double-stranded RNA viruses, transcription of particles with a double-stranded RNA genome alternates with replication of particles containing a single-stranded genome. In virions infecting some strains of Leishmania guyanensis the putative transcriptase and replicase activities of ...

journal_title：Molecular and biochemical parasitology

authors： Weeks RS,Patterson JL,Stuart K,Widmer G

更新日期：1992-06-01 00:00:00

abstract：:Reversible acetylation of core histones plays an important role in transcriptional regulation, cell cycle progression and developmental events. The acetylation state of histones is controlled by a dynamic equilibrium between activities of histone acetylase and deacetylase enzymes. Histone deacetylase (HDAC) was recent...

journal_title：Molecular and biochemical parasitology

authors： Joshi MB,Lin DT,Chiang PH,Goldman ND,Fujioka H,Aikawa M,Syin C

更新日期：1999-03-15 00:00:00

abstract：:The alpha and beta tubulin genes of Leishmania major were cloned and used to study the genomic organization, chromosomal location and transcription of tubulin genes in L. major. The number of beta tubulin isogenes was determined by hybridization of probes representing the 5' and 3' ends of the cloned beta tubulin cDNA...

journal_title：Molecular and biochemical parasitology

authors： Spithill TW,Samaras N

更新日期：1987-05-01 00:00:00

abstract：:Toxocariasis is a neglected disease, and its main etiological agent is the nematode Toxocara canis. Serological diagnosis is performed by an enzyme-linked immunosorbent assay using T. canis excretory and secretory (TES) antigens produced by in vitro cultivation of larvae. Identification of TES proteins can be useful f...

journal_title：Molecular and biochemical parasitology

authors： Sperotto RL,Kremer FS,Aires Berne ME,Costa de Avila LF,da Silva Pinto L,Monteiro KM,Caumo KS,Ferreira HB,Berne N,Borsuk S

更新日期：2017-01-01 00:00:00

abstract：:The binding kinetics of several benzimidazole compounds were determined with recombinant tubulin from benzimidazole-sensitive and -insensitive organisms. This study utilised the naturally occurring high efficacy of the benzimidazoles for the parasitic protozoa Giardia duodenalis and Encephalitozoon intestinalis, and l...

journal_title：Molecular and biochemical parasitology

authors： MacDonald LM,Armson A,Thompson AR,Reynoldson JA

更新日期：2004-11-01 00:00:00

abstract：:Antigenic variation in African trypanosomes proceeds by switching between different variant surface glycoprotein (VSG) molecules, whose extensive epitope differences enable evasion of antibody responses. Each trypanosome has approximately 1000 basic copy VSG genes inside chromosomes and a subset located at telomeres. ...

journal_title：Molecular and biochemical parasitology

authors： Graham VS,Barry JD

更新日期：1996-07-01 00:00:00

abstract：:GRA3 is a 30-kDa protein located inside the dense granules of Toxoplasma gondii. Following invasion and exocytosis of dense granules within the parasitophorous vacuole, GRA3 becomes associated with the parasitophorous vacuolar membrane (PVM) and extensions of the PVM which protrude into the cytoplasm. A partial cDNA e...

journal_title：Molecular and biochemical parasitology

authors： Bermudes D,Dubremetz JF,Achbarou A,Joiner KA

更新日期：1994-12-01 00:00:00

abstract：:After a single intravenous injection of suramin the rate of removal of the drug from the plasma into other tissue compartments of the rat is independent of initial concentration. The data can be fitted to the sum of two exponential functions, consistent with a two-compartment, open model system. Trypanosomes take up o...

journal_title：Molecular and biochemical parasitology

authors： Fairlamb AH,Bowman IB

更新日期：1980-10-01 00:00:00

abstract：:A gene-family, named sep, encoding small exported proteins conserved across Plasmodium species has been identified. SEP proteins (13-16 kDa) contain a predicted signal peptide at the NH(2)-terminus, an internal hydrophobic region and a polymorphic, low-complexity region at the carboxy-terminus. One member of the Plasm...

journal_title：Molecular and biochemical parasitology

authors： Birago C,Albanesi V,Silvestrini F,Picci L,Pizzi E,Alano P,Pace T,Ponzi M

更新日期：2003-02-01 00:00:00

abstract：:Genetic transformation of malaria parasites has been limited by the number of selectable markers available. For the rodent malaria parasite, Plasmodium berghei, only a single selection marker has been at hand, utilising the dihydrofolate reductase-thymidylate synthase gene from either P. berghei or Toxoplasma gondii t...

journal_title：Molecular and biochemical parasitology

authors： de Koning-Ward TF,Fidock DA,Thathy V,Menard R,van Spaendonk RM,Waters AP,Janse CJ

更新日期：2000-03-05 00:00:00

abstract：:Leishmania major promastigotes grown in late log phase were incubated with glucose as sole exogenous carbon source in the presence of 5% CO2 and the amounts of glucose consumed and of the major products formed--succinate, pyruvate, alanine, acetate, glycerol, and D-lactate--were measured as a function of pO2. Glucose ...

journal_title：Molecular and biochemical parasitology

authors： Keegan F,Blum JJ

更新日期：1990-03-01 00:00:00

abstract：:CK2 is a ubiquitous but enigmatic kinase. The difficulty in assigning a role to CK2 centers on the fact that, to date, no biologically relevant modulator of its function has been identified. One common theme revolves around a constellation of known substrates involved in growth control, compatible with its concentrati...

journal_title：Molecular and biochemical parasitology

authors： Jensen BC,Kifer CT,Brekken DL,Randall AC,Wang Q,Drees BL,Parsons M

更新日期：2007-01-01 00:00:00

abstract：:Two NAD(H)-dependent malate dehydrogenase (MDH) isoenzymes were detected in Phytomonas isolated from the lactiferous tubes of Euphorbia characias. The total specific activity in crude extracts using oxaloacetate as substrate was 3.3 U mg-1 of protein. The two isoenzymes had isoelectric points of 6.0 and 7.2, respectiv...

journal_title：Molecular and biochemical parasitology

authors： Uttaro AD,Opperdoes FR

更新日期：1997-10-01 00:00:00

abstract：:Toxoplasma gondii differentially expresses two forms of lactate dehydrogenase in tachyzoites and bradyzoites, respectively, designated LDH1 and LDH2. Previously it was demonstrated that LDH1 and LDH2 share a unique structural feature with LDH from the malarial parasite Plasmodium falciparum (pLDH), namely, the additio...

journal_title：Molecular and biochemical parasitology

authors： Dando C,Schroeder ER,Hunsaker LA,Deck LM,Royer RE,Zhou X,Parmley SF,Vander Jagt DL

更新日期：2001-11-01 00:00:00

abstract：:We have cloned and sequenced a homologue of the ring-infected erythrocyte surface antigen (RESA) gene from Plasmodium falciparum designated RESA-2. Two reading frames with high homology to exon 1 and exon 2 of RESA at both the nucleotide and amino acid levels were identified in the RESA-2 sequence. However, RESA-2 doe...

journal_title：Molecular and biochemical parasitology

authors： Cappai R,Kaslow DC,Peterson MG,Cowman AF,Anders RF,Kemp DJ

更新日期：1992-09-01 00:00:00

abstract：:The glycosylphosphatidylinositol phospholipase C (GPI-PLC) from Trypanosoma brucei is particularly effective in hydrolysing the GPI-anchors of some proteins. The enzyme is inhibited by Zn2+ and p-chloromercurylphenylsulphonic acid, both of which can act as sulphydryl reagents, suggesting that a cysteine residue may be...

journal_title：Molecular and biochemical parasitology

authors： Carnall N,Webb H,Carrington M

更新日期：1997-12-15 00:00:00

abstract：:Translation initiation factor eIF4F is essential for cap-dependent translation initiation in eukaryotes. eIF4F is a trimeric complex consisting of a scaffold protein eIF4G, cap-binding protein eIF4E and DEAD-box RNA helicase eIF4A. eIF4F binds to the 5' cap structure of the mRNA through eIF4E and facilitates the bindi...

journal_title：Molecular and biochemical parasitology

authors： Adedoja AN,McMahan T,Neal JP,Hamal Dhakal S,Jois S,Romo D,Hull K,Garlapati S

更新日期：2020-03-01 00:00:00

abstract：:We have previously provided evidence for a pathway in Plasmodium falciparum-infected erythrocytes, coined the parasitophorous duct pathway, which provides serum (macro)molecules direct access to intraerythrocytic parasites . The present study addresses the purity of the fluorescent macromolecules used to define the du...

journal_title：Molecular and biochemical parasitology

authors： Goodyer ID,Pouvelle B,Schneider TG,Trelka DP,Taraschi TF

更新日期：1997-07-01 00:00:00