当前位置： SCI文献检索 > MOLECULAR AND BIOCHEMICAL PARASITOLOGY期刊下所有文献 > Molecular cloning and characterisation of a developmentally regulated putative metallopeptidase present in a host protective extract of Haemonchus contortus.

Molecular cloning and characterisation of a developmentally regulated putative metallopeptidase present in a host protective extract of Haemonchus contortus.

Abstract：

:Antisera from lambs immunised with the Haemonchus contortus integral membrane protein complex, Haemonchus galactose-containing glycoprotein (H-gal-GP), the lambs being refractory to subsequent challenge, were used to identify several clones from an adult H. contortus lambda gt11 cDNA library. Using gene-specific oligonucleotide primers in conjunction with primers directed to a conserved nematode Spliced Leader (SL) sequence and to the polyA+ tail of mRNA, the remaining 5' and 3' sequences of one of these clones, metallopeptidase-1 (MEP1), were amplified. The 2.4 kb full-length coding sequences was subsequently amplified in a single reaction. Sequence analysis identified MEP1 as encoding a putative zinc metallopeptidase, which shared limited homology with the mammalian type II integral membrane protein neutral endopeptidase (NEP). Southern blotting indicated that MEP1 belonged to a multigene family. MEP1 was expressed in bacteria as a glutathione-S-transferase (GST) fusion protein, and a specific antiserum raised in sheep. This antiserum recognised several polypeptide components of H-gal-GP. Immunolocalisation studies showed that MEP1 encoded a protein located on the luminal surface of the nematode gut. Both MEP1 mRNA and protein are developmentally regulated with expression being limited to the blood-feeding stages of H. contortus.

journal_name

Mol Biochem Parasitol

journal_title

Molecular and biochemical parasitology

authors

Redmond DL,Knox DP,Newlands G,Smith WD

doi

10.1016/s0166-6851(96)02812-5

subject

Has Abstract

pub_date

1997-03-01 00:00:00

pages

77-87

issue

1

eissn

0166-6851

issn

1872-9428

pii

S0166-6851(96)02812-5

journal_volume

85

pub_type

杂志文章

相关文献

MOLECULAR AND BIOCHEMICAL PARASITOLOGY文献大全
  • Characterization of the gene family encoding a host-protective antigen of the tapeworm Taenia ovis.

    abstract：:Genomic structure has been determined for a gene encoding a host-protective antigen of the parasitic platyhelminth Taenia ovis. An incomplete cDNA, known as 45W, encodes the protective antigen. Southern hybridisation experiments using 45W cDNA as a probe, revealed that the 45W gene was a member of a multigene family. ...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(94)00104-u

    authors： Waterkeyn JG,Lightowlers MW,Coppel R,Cowman AF

    更新日期：1995-07-01 00:00:00

  • Isolation of lambda amp3 genomic recombinants coding for antigens of Eimeria tenella.

    abstract：:Eimeria are the causative agents of coccidosis, a disease which is of world wide economic importance in the poultry industry. Immunity resulting from infection is species-specific and both antibody and cell-mediated responses have been implicated. As an initial step in the development of a genetically-engineered vacci...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(87)90071-5

    authors： Clarke LE,Messer LI,Greenwood NM,Wisher MH

    更新日期：1987-01-02 00:00:00

  • Crystal structures of Toxoplasma gondii pterin-4a-carbinolamine dehydratase and comparisons with mammalian and parasite orthologues.

    abstract：:The enzyme pterin-4a-carbinolamine dehydratase (PCD) is important for the recycling of pterins within eukaryotic cells. A recombinant expression system for PCD from the apicomplexan parasite Toxoplasma gondii has been prepared, the protein purified and crystallised. Single crystal X-ray diffraction methods have produc...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2007.12.002

    authors： Cameron S,Fyffe SA,Goldie S,Hunter WN

    更新日期：2008-04-01 00:00:00

  • The mitochondrial phosphate carrier TbMCP11 is essential for mitochondrial function in the procyclic form of Trypanosoma brucei.

    abstract：:Conserved amongst all eukaryotes is a family of mitochondrial carrier proteins (SLC25A) responsible for the import of various solutes across the inner mitochondrial membrane. We previously reported that the human parasite Trypanosoma brucei possesses 26 SLC25A proteins (TbMCPs) amongst which two, TbMCP11 and TbMCP8, w...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2020.111275

    authors： Gao F,Voncken F,Colasante C

    更新日期：2020-05-01 00:00:00

  • SYBR, TaqMan, or both: highly sensitive, non-invasive detection of Cardicola blood fluke species in Southern Bluefin Tuna (Thunnus maccoyii).

    abstract：:Three species of blood fluke from the genus Cardicola are known to parasitize and cause disease in Bluefin Tunas--C. forsteri, C. orientalis, and C. opisthorchis. Although initially believed to be separated by geography and host specificity, recent identification of at least two Cardicola spp. concurrently present wit...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2013.07.002

    authors： Polinski M,Hamilton DB,Nowak B,Bridle A

    更新日期：2013-09-01 00:00:00

  • Cloning and functional identification of a novel annexin subfamily in Cysticercus cellulosae.

    abstract：:A novel cDNA was isolated from a library of Cysticercus cellulosae by immunological screening. Sequence analysis reveals that it has typical annexin structures. However, since its N-terminus is distinct and the core domain only has 32-44% amino acid identity to the tetrads of other annexins, the cDNA encodes a member ...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/s0166-6851(01)00383-8

    authors： Hongli Y,Shuhan S,Ruiwen C,Yingjun G

    更新日期：2002-01-01 00:00:00

  • Isolation and partial sequence of a collagen gene from the human filarial parasite Brugia malayi.

    abstract：:We report the isolation and sequence of a part of a gene encoding a collagen from the genome of the human filarial parasite Brugia malayi. The deduced amino acid sequence of the sequenced portion of this gene, which we have designated BmCol1, differs from the most catalogued nematode collagens in that it is composed p...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(91)90027-4

    authors： Caulagi VR,Werner C,Rajan TV

    更新日期：1991-03-01 00:00:00

  • Variant surface glycoproteins of Trypanosoma congolense bloodstream and metacyclic forms are anchored by a glycolipid tail.

    abstract：:The variant surface glycoproteins (VSGs) of both metacyclic and bloodstream forms of Trypanosoma congolense are shown to be anchored to the plasma membrane through a glycolipid similar to that found in Trypanosoma brucei. Release of soluble VSG from both metacyclic and bloodstream forms is associated with the exposure...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(87)90045-4

    authors： Ross CA,Cardoso de Almeida ML,Turner MJ

    更新日期：1987-01-15 00:00:00

  • A gene-family encoding small exported proteins is conserved across Plasmodium genus.

    abstract：:A gene-family, named sep, encoding small exported proteins conserved across Plasmodium species has been identified. SEP proteins (13-16 kDa) contain a predicted signal peptide at the NH(2)-terminus, an internal hydrophobic region and a polymorphic, low-complexity region at the carboxy-terminus. One member of the Plasm...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/s0166-6851(02)00275-x

    authors： Birago C,Albanesi V,Silvestrini F,Picci L,Pizzi E,Alano P,Pace T,Ponzi M

    更新日期：2003-02-01 00:00:00

  • Autophagy in parasitic protists: unique features and drug targets.

    abstract：:Eukaryotic cells can degrade their own components, cytosolic proteins and organelles, using dedicated hydrolases contained within the acidic interior of their lysosomes. This degradative process, called autophagy, is used under starvation conditions to recycle redundant or less important macromolecules, facilitates me...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章,评审

    doi：10.1016/j.molbiopara.2011.02.003

    authors： Brennand A,Gualdrón-López M,Coppens I,Rigden DJ,Ginger ML,Michels PA

    更新日期：2011-06-01 00:00:00

  • A unique Rab GTPase, EhRabA, of Entamoeba histolytica, localizes to the leading edge of motile cells.

    abstract：:Entamoeba histolytica, an enteric protozoan parasite, infects 10% of the world's population leading to 50 million cases of invasive amoebiasis annually. Parasite vesicle trafficking and motility, which relies on vesicle trafficking to deliver membrane and membrane components to the leading edge, are important for viru...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2004.02.002

    authors： Welter BH,Temesvari LA

    更新日期：2004-06-01 00:00:00

  • Filarial parasites contain a ras homolog of the TC4/ran/Spil family.

    abstract：:We have isolated and characterized a gene encoding a novel GTP-binding protein of the GTPase superfamily in the filarial parasites Brugia malayi and Onchocerca volvulus. The deduced amino acid sequence of the cloned molecule has approximately 30% overall homology to ras proteins and approximately 90% homology to the '...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(92)90175-j

    authors： Dissanayake S,Xu M,Piessens WF

    更新日期：1992-12-01 00:00:00

  • Recent independent evolution of msp1 polymorphism in Plasmodium vivax and related simian malaria parasites.

    abstract：:The Plasmodium MSP-1 is a promising malaria vaccine candidate. However, the highly polymorphic nature of the MSP-1 gene (msp1) presents a potential obstacle for effective vaccine development. To investigate the evolutionary history of msp1 polymorphism in P. vivax, we construct phylogenetic trees of msp1 from P. vivax...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2007.07.002

    authors： Tanabe K,Escalante A,Sakihama N,Honda M,Arisue N,Horii T,Culleton R,Hayakawa T,Hashimoto T,Longacre S,Pathirana S,Handunnetti S,Kishino H

    更新日期：2007-11-01 00:00:00

  • Population genetic analysis and sub-structuring in Babesia bovis.

    abstract：:The tick-borne protozoan parasite, Babesia bovis is one of the causes of bovine babesiosis, an economically important disease of cattle in tropical and sub-tropical countries. Using the recently published genome sequence of the parasite, we developed a panel of eight mini- and micro-satellite markers and used these to...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2011.02.002

    authors： Simuunza M,Bilgic H,Karagenc T,Syakalima M,Shiels B,Tait A,Weir W

    更新日期：2011-06-01 00:00:00

  • Surface epitope localization and gene structure of a Babesia bovis 44-kilodalton variable merozoite surface antigen.

    abstract：:Variation of Babesia bovis merozoite surface antigens occurs among geographic strains of the parasite. In this and a concurrent report, we investigate this variation at the gene and protein level. Using a monoclonal antibody (mAb 23/70.174), B. bovis gene sequences were identified that encoded a surface epitope of a 4...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(92)90128-7

    authors： Jasmer DP,Reduker DW,Hines SA,Perryman LE,McGuire TC

    更新日期：1992-10-01 00:00:00

  • Electrophoretic analyses of myofibrillar proteins from the body wall muscles of Ascaris suum.

    abstract：:A myofibrillar protein extract has been isolated from the muscle of Ascaris suum. Two-dimensional electrophoresis of this extract revealed that the myosin light chain 1 (ALC1) migrates as 3 components with approximate isoelectric points in the range of 5.3-5.6. The most acidic component of ALC1 appeared to be phosphor...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(81)90007-4

    authors： Srihari T,Wiehrer W,Pette D,Harris BG

    更新日期：1981-06-01 00:00:00

  • A conserved domain targets exported PHISTb family proteins to the periphery of Plasmodium infected erythrocytes.

    abstract：:During blood-stage infection, malaria parasites export numerous proteins to the host erythrocyte. The Poly-Helical Interspersed Sub-Telomeric (PHIST) proteins are an exported family that share a common 'PRESAN' domain, and include numerous members in Plasmodium falciparum, Plasmodium vivax and Plasmodium knowlesi. In ...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2014.07.011

    authors： Tarr SJ,Moon RW,Hardege I,Osborne AR

    更新日期：2014-08-01 00:00:00

  • Optical map of the genotype A1 WB C6 Giardia lamblia genome isolate.

    abstract：:The Giardia lamblia genome consists of 12 Mb divided among 5 chromosomes ranging in size from approximately 1 to 4 Mb. The assembled contigs of the genotype A1 isolate, WB, were previously mapped along the 5 chromosomes on the basis of hybridization of plasmid clones representing the contigs to chromosomes separated b...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2011.07.008

    authors： Perry DA,Morrison HG,Adam RD

    更新日期：2011-12-01 00:00:00

  • Identification of peripheral membrane proteins associated with the tubo-vesicular network of Plasmodium falciparum infected erythrocytes.

    abstract：:During intracellular development of the malarial parasite numerous membranous vesicles appear in the infected erythrocyte cytoplasm between the parasitophorous vacuolar membrane (PVM) and the erythrocyte plasma membrane. In this study we describe the characterization of a monoclonal antibody which recognizes two major...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/s0166-6851(97)00206-5

    authors： Martinez SL,Clavijo CA,Winograd E

    更新日期：1998-03-15 00:00:00

  • Loss of matrix metalloproteinase 9 activity in Theileria annulata-attenuated cells is at the transcriptional level and is associated with differentially expressed AP-1 species.

    abstract：:The schizont stage of the protozoan parasite Theileria annulata reversibly transforms bovine monocytes into an immortalised and metastatic state. We have been studying T. annulata induction of host matrix metalloproteinases (MMP) which are involved in parasite dissemination and pathogenesis. We have observed that prol...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/s0166-6851(99)00213-3

    authors： Adamson R,Logan M,Kinnaird J,Langsley G,Hall R

    更新日期：2000-02-25 00:00:00

  • A surface-labeled 18 kilodalton antigen in Schistosoma mansoni.

    abstract：:A mild surface-labeling procedure was applied to various developmental stages of Schistosoma mansoni. An 18 kDa protein was preferentially labeled in freshly transformed schistosomula. The labeled protein was equally present on skin-penetrated and mechanically prepared schistosomula and it disappeared upon digestion o...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(86)90050-2

    authors： Liberti P,Festucci A,Ruppel A,Gigante S,Cioli D

    更新日期：1986-01-01 00:00:00

  • Characterisation of the gene encoding a 104-kilodalton microneme-rhoptry protein of Theileria parva.

    abstract：:A neutralizing antiserum, C16, raised against sporozoites of Theileria parva parva was used to screen a lambda gt11 expression library of T. parva parva (Muguga) genomic DNA fragments. Proteins encoded by one phage clone, lambda TpS-17, were reactive with the C16 antiserum. Detailed characterisation of the DNA insert ...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(90)90007-9

    authors： Iams KP,Young JR,Nene V,Desai J,Webster P,ole-MoiYoi OK,Musoke AJ

    更新日期：1990-02-01 00:00:00

  • Characterization of beta-tubulin genes in hookworms and investigation of resistance-associated mutations using real-time PCR.

    abstract：:Human hookworms (Ancylostoma duodenale, Necator americanus) are a major cause of malnutrition and anemia, particularly in children, and high worm burdens can lead to stunted growth and mental retardation. Mass drug administration (MDA) with benzimidazole (BZ) anthelmintics has the potential to greatly reduce morbidity...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2007.07.019

    authors： Schwenkenbecher JM,Albonico M,Bickle Q,Kaplan RM

    更新日期：2007-12-01 00:00:00

  • Immunochemical characterisation of Schistosoma mansoni glycolipid antigens.

    abstract：:The aim of this study was to investigate the occurrence, distribution and immunochemical properties of antibody-defined carbohydrate epitopes in neutral glycolipid fractions of Schistosoma mansoni eggs, cercariae and adults. The amount of extractable, antigenic, neutral glycolipids was lowest in adult worms, increasin...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/s0166-6851(99)00123-1

    authors： Wuhrer M,Dennis RD,Doenhoff MJ,Bickle Q,Lochnit G,Geyer R

    更新日期：1999-10-15 00:00:00

  • The T687G SNP in a P-glycoprotein gene of Fasciola hepatica is not associated with resistance to triclabendazole in two resistant Australian populations.

    abstract：:Triclabendazole (TCBZ) is widely used for control of Fasciola hepatica (liver fluke) in animals and humans and resistance to this drug is now widespread. However, the mechanism of resistance to TCBZ is not known. A T687G single nucleotide polymorphism (SNP) in a P-glycoprotein gene was proposed as a molecular marker f...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2014.11.006

    authors： Elliott TP,Spithill TW

    更新日期：2014-11-01 00:00:00

  • Trypanosoma cruzi attachment to lymphocyte muscarinic cholinergic and beta adrenergic receptors modulates intracellular signal transduction.

    abstract：:Plasma membrane vesicles of Trypanosoma cruzi (PMVs) formed saturation binding isotherms with naive murine T lymphocytes. Parasite membrane attachment to the muscarinic cholinergic receptors of Lyt 2.2+T cells (suppressor cells) resulted in the synthesis of cGMP, attenuation of cAMP levels and in the secretion of pros...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(91)90151-u

    authors： Borda ES,Sterin-Borda LJ,Pascual JO,Gorelik G,Felix JC,von Kreuter BF,Santos-Buch CA

    更新日期：1991-07-01 00:00:00

  • Synthesis of merozoite proteins and glycoproteins during the schizogony of Plasmodium falciparum.

    abstract：:We have investigated the protein and glycoprotein content of Plasmodium falciparum merozoites by metabolically labeling cultures of schizont-stage parasites with [35S]methionine or with [3H]glucosamine followed by incubation in nonradioactive medium to allow the schizonts to mature into merozoites, infect new erythroc...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(84)90030-6

    authors： Howard RF,Reese RT

    更新日期：1984-03-01 00:00:00

  • Isolation and functional characterization of snail hemocyte-modulating polypeptide from primary sporocysts of Schistosoma mansoni.

    abstract：:Infection with larval trematodes has been shown to inhibit several snail-host defences, including hemocyte phagocytosis, cytotoxicity, motility, and adherence. Certain plasma factors which mediate snail defence responses, and which may be produced by host hemocytes, also appear to be altered by these parasites. In thi...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/0166-6851(91)90124-o

    authors： Lodes MJ,Connors VA,Yoshino TP

    更新日期：1991-11-01 00:00:00

  • Translation initiation factors GleIF4E2 and GleIF4A can interact directly with the components of the pre-initiation complex to facilitate translation initiation in Giardia lamblia.

    abstract：:Translation initiation factor eIF4F is essential for cap-dependent translation initiation in eukaryotes. eIF4F is a trimeric complex consisting of a scaffold protein eIF4G, cap-binding protein eIF4E and DEAD-box RNA helicase eIF4A. eIF4F binds to the 5' cap structure of the mRNA through eIF4E and facilitates the bindi...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/j.molbiopara.2020.111258

    authors： Adedoja AN,McMahan T,Neal JP,Hamal Dhakal S,Jois S,Romo D,Hull K,Garlapati S

    更新日期：2020-03-01 00:00:00

  • Characterisation of the loci encoding the glutamic acid and alanine rich protein of Trypanosoma congolense.

    abstract：:We have characterised the organisation of genes encoding the glutamate and alanine rich protein (GARP) surface coat of the procyclic and epimastigote stages of Trypanosoma congolense in the tsetse fly. The GARP genes are arranged at two, possibly physically linked, loci, one of which exhibits allelic variation. One lo...

    journal_title：Molecular and biochemical parasitology

    pub_type： 杂志文章

    doi：10.1016/s0166-6851(99)00190-5

    authors： Rangarajan D,Harvey TI,Barry JD

    更新日期：2000-02-05 00:00:00