Molecular cloning and characterisation of a developmentally regulated putative metallopeptidase present in a host protective extract of Haemonchus contortus.


:Antisera from lambs immunised with the Haemonchus contortus integral membrane protein complex, Haemonchus galactose-containing glycoprotein (H-gal-GP), the lambs being refractory to subsequent challenge, were used to identify several clones from an adult H. contortus lambda gt11 cDNA library. Using gene-specific oligonucleotide primers in conjunction with primers directed to a conserved nematode Spliced Leader (SL) sequence and to the polyA+ tail of mRNA, the remaining 5' and 3' sequences of one of these clones, metallopeptidase-1 (MEP1), were amplified. The 2.4 kb full-length coding sequences was subsequently amplified in a single reaction. Sequence analysis identified MEP1 as encoding a putative zinc metallopeptidase, which shared limited homology with the mammalian type II integral membrane protein neutral endopeptidase (NEP). Southern blotting indicated that MEP1 belonged to a multigene family. MEP1 was expressed in bacteria as a glutathione-S-transferase (GST) fusion protein, and a specific antiserum raised in sheep. This antiserum recognised several polypeptide components of H-gal-GP. Immunolocalisation studies showed that MEP1 encoded a protein located on the luminal surface of the nematode gut. Both MEP1 mRNA and protein are developmentally regulated with expression being limited to the blood-feeding stages of H. contortus.


Mol Biochem Parasitol


Redmond DL,Knox DP,Newlands G,Smith WD




Has Abstract


1997-03-01 00:00:00














  • Molecular cloning, genomic structure and localization in a blood stage antigen of Plasmodium falciparum characterized by a serine stretch.

    abstract::Two short DNA segments were isolated by screening of a lambda gt11 library from Plasmodium falciparum schizont cDNA with an antiserum against the 140 kDa protein, which confers protective immunity to monkeys. The segments were used to identify a genomic fragment which carries the entire coding sequence for a protein o...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Knapp B,Hundt E,Nau U,Küpper HA

    更新日期:1989-01-01 00:00:00

  • Properties of the acid thiol proteinase from Schistosoma mansoni adults.

    abstract::Seven beta-naphthylamine-linked peptides were tested as substrates for a previously described thiol proteinase of adult Schistosoma mansoni. The enzyme was not active on carbobenoxy-arginyl-arginyl-4-methoxy-2-naphthylamide and carbobenzoxy-alanyl-arginyl-arginyl-4-methoxy-2-naphthylamide. Enzyme activity was maximal ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Dresden MH,Rutledge ML,Chappell CL

    更新日期:1981-11-01 00:00:00

  • Episomal and integrated maintenance of foreign DNA in Giardia lamblia.

    abstract::Giardia lamblia is an early diverging eukaryote which causes gastrointestinal disease throughout the world. Different subgroups of Giardia have been defined based on several biochemical and genetic criteria. We have developed a method for stably introducing DNA into the nuclei of the parasite using puromycin acetyltra...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Singer SM,Yee J,Nash TE

    更新日期:1998-04-01 00:00:00

  • An epitope recognised by inhibitory monoclonal antibodies that react with a 51 kilodalton merozoite surface antigen in Plasmodium falciparum.

    abstract::Monoclonal antibodies designated 8G10/48 and 9E3/48 raised against mature asexual blood stages of Plasmodium falciparum inhibit parasite growth in vitro. Both antibodies bind to an epitope which includes the linear sequence Ser Thr Asn Ser and which is present in a cDNA clone from a P. falciparum expression library. T...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Epping RJ,Goldstone SD,Ingram LT,Upcroft JA,Ramasamy R,Cooper JA,Bushell GR,Geysen HM

    更新日期:1988-02-01 00:00:00

  • Characterization of natural antisense transcripts arisen from the locus encoding Toxoplasma gondii ubiquitin-like protease.

    abstract::Natural antisense transcripts (NATs) are non-protein coding RNAs that could play an important role in regulating the expression of their counterpart protein encoding sense transcript. Although NATs are widespread in most eukaryotic genomes, very little is known about their functions. This study focuses on gaining a be...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Fahim A,Afrin F,Wen G,Ananvoranich S

    更新日期:2020-11-01 00:00:00

  • Efficient extraction and translation of Plasmodium falciparum messenger RNA.

    abstract::A convenient method was employed for the efficient extraction of total RNA from Plasmodium falciparum. By depleting the total RNA of tRNA, it was shown that P. falciparum or P. lophurae tRNAs markedly stimulate the translation of P. falciparum mRNA in the rabbit reticulocyte cell-free system. Analysis of the cell-free...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Wallach M

    更新日期:1982-12-01 00:00:00

  • Identification and functional characterization of a member of the PUR-alpha family from Schistosoma mansoni.

    abstract::Schistosoma mansoni p14 gene encodes an eggshell precursor that is expressed only in vitelline cells of mature female worms in response to a male stimulus. The upstream region of the p14 gene contains several potential cis-acting regulatory sequences. We used the upstream region of the p14 gene as bait in a yeast-one-...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Fantappie MR,Osman A,Niles EG,LoVerde PT

    更新日期:2000-10-01 00:00:00

  • Chemiluminescence enhancement by trypanocidal drugs and by inhibitors of antioxidant enzymes in Trypanosoma cruzi.

    abstract::The spontaneous emission of chemiluminescence by Trypanosoma cruzi epimastigotes was 133 +/- 5 counts s-1 (mg protein)-1. The measured intracellular steady state concentration of hydrogen peroxide in the same cells was 1.5 +/- 0.5 microM. These two values are about 12- and 15-times higher than the corresponding ones f...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Giulivi C,Turrens JF,Boveris A

    更新日期:1988-09-01 00:00:00

  • The mitochondrial phosphate carrier TbMCP11 is essential for mitochondrial function in the procyclic form of Trypanosoma brucei.

    abstract::Conserved amongst all eukaryotes is a family of mitochondrial carrier proteins (SLC25A) responsible for the import of various solutes across the inner mitochondrial membrane. We previously reported that the human parasite Trypanosoma brucei possesses 26 SLC25A proteins (TbMCPs) amongst which two, TbMCP11 and TbMCP8, w...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Gao F,Voncken F,Colasante C

    更新日期:2020-05-01 00:00:00

  • Genetic variability and evolution of the Schistosoma genome analysed by using random amplified polymorphic DNA markers.

    abstract::The usefulness of random amplified polymorphic DNA markers (RAPD) was assayed in an attempt to discriminate among species, strains and individuals within the genus Schistosoma. Depending on the species, 40-50 arbitrary decamer oligonucleotides were used as primers to amplify total DNA by the polymerase chain reaction ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Barral V,This P,Imbert-Establet D,Combes C,Delseny M

    更新日期:1993-06-01 00:00:00

  • Sequences with high propensity to form G-quartet structures in kinetoplast DNA from Phytomonas serpens.

    abstract::Naturally occurring sequences containing repetitive guanine motifs have the potential to form tetraplex DNA. Phytomonas serpens minicircle DNA shows some regions where one strand is composed mainly of G and T (GT regions). These regions contain several stretches of contiguous guanines. An oligonucleotide was construct...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Sá-Carvalho D,Traub-Cseko YM

    更新日期:1995-06-01 00:00:00

  • Complementation of Saccharomyces cerevisiaepik1ts by a phosphatidylinositol 4-kinase from Plasmodium falciparum.

    abstract::Phosphoinositides comprise a group of essential phospholipids that control a variety of cellular functions. In the case of the human malaria parasite Plasmodium falciparum, phosphoinositides have been shown to trigger exflagellation and to affect haemoglobin endocytosis and maturation of the parasite's digestive vacuo...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Krüger T,Sanchez CP,Lanzer M

    更新日期:2010-08-01 00:00:00

  • A (Ca(2+)-Mg2+)ATPase from Schistosoma mansoni is coupled to an active transport of calcium.

    abstract::The (Ca(2+)-Mg2+)ATPase activity in microsomes of Schistosoma mansoni is fully inhibited by vanadate (I50 = 2.5 microM). 45Ca2+ is accumulated within microsomal vesicles in an ATP-dependent process that is enhanced 5-fold in the presence of 40 mM phosphate. Accumulated 45Ca2+ is rapidly released by 5 microM of the Ca2...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Cunha VM,Meyer-Fernandes JR,Noël F

    更新日期:1992-06-01 00:00:00

  • Dominant lethality and insect population control.

    abstract::Recent advances in insect genetic engineering have opened up new possibilities in the genetic control of insect vectors of human diseases. We outline the current state of the sterile insect technique and show how the use of engineered dominant lethals can greatly increase the effectiveness of this approach. We conside...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Alphey L,Andreasen M

    更新日期:2002-05-01 00:00:00

  • Context-dependent roles of the Entamoeba histolytica core promoter element GAAC in transcriptional activation and protein complex assembly.

    abstract::Transcriptional control of the hgl5 gene of Entamoeba histolytica is mediated through an unusual core promoter composed of TATA, GAAC and Initiator elements. In the hgl5 promoter the GAAC element (AATGAACT) determines the site and rate of transcription initiation. Here we tested the role of the GAAC element in transcr...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Singh U,Gilchrist CA,Schaenman JM,Rogers JB,Hockensmith JW,Mann BJ,Petri WA

    更新日期:2002-03-01 00:00:00

  • Demonstration of glycosomes (microbodies) in the Bodonid flagellate Trypanoplasma borelli (Protozoa, Kinetoplastida).

    abstract::Homogenates of Trypanoplasma borelli were subjected to subcellular fractionation by sequential differential and isopycnic centrifugation in sucrose. Glycerol-3-phosphate dehydrogenase and the glycolytic enzymes, glucosephosphate isomerase and triosephosphate isomerase, as well as the peroxisomal marker enzyme catalase...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Opperdoes FR,Nohynkova E,Van Schaftingen E,Lambeir AM,Veenhuis M,Van Roy J

    更新日期:1988-08-01 00:00:00

  • The two mechanisms for antigenic variation in Trypanosoma brucei are independent processes.

    abstract::Antigenic switching in Trypanosoma brucei can occur either by the production of a telomeric copy of a variant surface glycoprotein (VSG) gene through a gene conversion mechanism or by the nonduplicative activation of a telomeric VSG gene. The 5 VSG gene telomeric copy that is expressed in IsTaR 1 variant antigenic typ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Aline RF Jr,Stuart K

    更新日期:1985-06-01 00:00:00

  • Purification and properties of Plasmodium falciparum malate dehydrogenase.

    abstract::Asexual intraerythrocytic Plasmodium falciparum were shown to have a single isoenzyme of malate dehydrogenase. This malate dehydrogenase was purified to apparent homogeneity using a three-step purification protocol. The parasite malate dehydrogenase had an apparent subunit molecular weight of 32 kDa, a pH optimum of 7...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Lang-Unnasch N

    更新日期:1992-01-01 00:00:00

  • Characterization of macromolecular transport pathways in malaria-infected erythrocytes.

    abstract::We have previously provided evidence for a pathway in Plasmodium falciparum-infected erythrocytes, coined the parasitophorous duct pathway, which provides serum (macro)molecules direct access to intraerythrocytic parasites . The present study addresses the purity of the fluorescent macromolecules used to define the du...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Goodyer ID,Pouvelle B,Schneider TG,Trelka DP,Taraschi TF

    更新日期:1997-07-01 00:00:00

  • Isolation, sequencing and expression of the gene encoding hypoxanthine-guanine-xanthine phosphoribosyltransferase of Tritrichomonas foetus.

    abstract::We have cloned and expressed the full-length gene encoding the hypoxanthine-guanine-xanthine phosphoribosyltransferase (HGXPRTase) from the anaerobic protozoan parasite Tritrichomonas foetus. This enzyme is essential in nucleic acid metabolism of T. foetus because the parasite is unable to synthesize purine nucleotide...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Chin MS,Wang CC

    更新日期:1994-02-01 00:00:00

  • Characterization of the complete mitogenome of Centrorhynchus clitorideus (Meyer, 1931) (Palaeacanthocephala: Centrorhynchidae), the largest mitochondrial genome in Acanthocephala, and its phylogenetic implications.

    abstract::Species of Centrorhynchus (Polymorphida: Centrorhynchidae) commonly parasitize various falconiform and strigiform birds worldwide. In the present study, the complete mitochondrial (mt) genome sequences of Centrorhynchus clitorideus was sequenced and annotated for the first time based on specimens collected from the li...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Muhammad N,Suleman,Khan MS,Li L,Zhao Q,Ullah H,Zhu XQ,Ma J

    更新日期:2020-05-01 00:00:00

  • Long-range restriction maps of size-variable homologous chromosomes in Leishmania infantum.

    abstract::In order to clarify the interpretation of molecular karyotype polymorphisms in Leishmania, the three smallest chromosomes from five cloned strains of Leishmania infantum were identified by chromosome-specific anonymous DNA probes. The presence in the same clone of homologous chromosomes of a different size was demonst...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Blaineau C,Bastien P,Rioux JA,Roizès G,Pagès M

    更新日期:1991-06-01 00:00:00

  • Molecular cloning, organellar targeting and developmental expression of mitochondrial chaperone HSP60 in Toxoplasma gondii.

    abstract::The obligate intracellular protozoan parasite Toxoplasma gondii has a single tubular mitochondrion. During infection, it recruits the host cell's mitochondria abutting to the intracellular vacuole, that contains the parasites. The respective contribution of host and parasitic mitochondria in the intracellular growth o...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Toursel C,Dzierszinski F,Bernigaud A,Mortuaire M,Tomavo S

    更新日期:2000-12-01 00:00:00

  • Characterization of the gene family encoding a host-protective antigen of the tapeworm Taenia ovis.

    abstract::Genomic structure has been determined for a gene encoding a host-protective antigen of the parasitic platyhelminth Taenia ovis. An incomplete cDNA, known as 45W, encodes the protective antigen. Southern hybridisation experiments using 45W cDNA as a probe, revealed that the 45W gene was a member of a multigene family. ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Waterkeyn JG,Lightowlers MW,Coppel R,Cowman AF

    更新日期:1995-07-01 00:00:00

  • Conservation of the Plasmodium falciparum sporozoite surface protein gene, STARP, in field isolates and distinct species of Plasmodium.

    abstract::The extent of structural conservation of the Plasmodium falciparum sporozoite surface protein gene, STARP, recently characterized in the T9/96 clone, has been analyzed using the polymerase chain reaction. Results from Ivory Coast and Thai clones, field isolates originating from Brazil and Kenya and laboratory-maintain...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Fidock DA,Sallenave-Sales S,Sherwood JA,Gachihi GS,Ferreira-da-Cruz MF,Thomas AW,Druilhe P

    更新日期:1994-10-01 00:00:00

  • Homologous telomeric sequences are present in different species of the genus Plasmodium.

    abstract::The telomeric sequence cloned from Plasmodium berghei (see M. Ponzi et al. (1985) EMBO J. 4, 2991-2995) was tested for species specificity. A telomeric and a subtelomeric fragment of the cloned insert served as separate, labelled probes on pulsed field gradient electrophoretical patterns and on genomic digests from th...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Dore E,Pace T,Ponzi M,Scotti R,Frontali C

    更新日期:1986-11-01 00:00:00

  • The role of N-linked carbohydrates in the antigenicity of Taenia solium metacestode glycoproteins of 12, 16 and 18 kD.

    abstract::The glycoproteins of 12-28 kD from Taenia solium metacestodes provide a high specificity and sensitivity for the serological diagnosis of the central nervous system infection, neurocysticercosis. Their widespread use as antigens for routine serological assays will require their production in large and reproducible amo...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Obregón-Henao A,Gil DL,Gómez DI,Sanzón F,Teale JM,Restrepo BI

    更新日期:2001-05-01 00:00:00

  • A Trypanosoma brucei mutant resistant to alpha-difluoromethylornithine.

    abstract::Procyclic Trypanosoma brucei brucei strain 366D is susceptible to DL-alpha-difluoromethylornithine (DFMO) with an in vitro ED50 value of 225 microM. A mutant of the procyclic strain resistant to 20 mM of DFMO was isolated by serial in vitro passages of the organisms in increasing concentrations of the drug. Drug resis...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Phillips MA,Wang CC

    更新日期:1987-01-02 00:00:00

  • The effect of over-expression of the alternative oxidase in the procyclic forms of Trypanosoma brucei.

    abstract::Trypanosome alternative oxidase (TAO) is the cyanide-resistant but SHAM-sensitive terminal oxidase of the mitochondrial electron transport chain in African trypanosomes. The bloodstream forms of Trypanosoma brucei lack cytochromes and respire exclusively via TAO. On the other hand, the insect, or procyclic form posses...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Walker R Jr,Saha L,Hill GC,Chaudhuri M

    更新日期:2005-02-01 00:00:00

  • Cloning and functional identification of a novel annexin subfamily in Cysticercus cellulosae.

    abstract::A novel cDNA was isolated from a library of Cysticercus cellulosae by immunological screening. Sequence analysis reveals that it has typical annexin structures. However, since its N-terminus is distinct and the core domain only has 32-44% amino acid identity to the tetrads of other annexins, the cDNA encodes a member ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hongli Y,Shuhan S,Ruiwen C,Yingjun G

    更新日期:2002-01-01 00:00:00