Loss of matrix metalloproteinase 9 activity in Theileria annulata-attenuated cells is at the transcriptional level and is associated with differentially expressed AP-1 species.


:The schizont stage of the protozoan parasite Theileria annulata reversibly transforms bovine monocytes into an immortalised and metastatic state. We have been studying T. annulata induction of host matrix metalloproteinases (MMP) which are involved in parasite dissemination and pathogenesis. We have observed that prolonged in vitro culture of T. annulata-infected cell lines results in their attenuation and this process is associated with alterations in both host and parasite gene expression. In particular, a loss in bovine MMP expression in later passage cultures suggests that these parasite-induced MMPs are virulence factors. As a means to further our understanding of the attenuation process we examine in detail the parasite-induced differential expression of one particular bovine proteinase, MMP9, in non-attenuated (p58) and attenuated (p158) passage levels of the Ode vaccine line. We show here that MMP9 expression is regulated at the transcriptional level and we suggest that a particular parasite-induced AP-1 recognition transcription factor present in the Ode non-attenuated line may have a role to play in the expression of this host gene.


Mol Biochem Parasitol


Adamson R,Logan M,Kinnaird J,Langsley G,Hall R




Has Abstract


2000-02-25 00:00:00














  • Recent independent evolution of msp1 polymorphism in Plasmodium vivax and related simian malaria parasites.

    abstract::The Plasmodium MSP-1 is a promising malaria vaccine candidate. However, the highly polymorphic nature of the MSP-1 gene (msp1) presents a potential obstacle for effective vaccine development. To investigate the evolutionary history of msp1 polymorphism in P. vivax, we construct phylogenetic trees of msp1 from P. vivax...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Tanabe K,Escalante A,Sakihama N,Honda M,Arisue N,Horii T,Culleton R,Hayakawa T,Hashimoto T,Longacre S,Pathirana S,Handunnetti S,Kishino H

    更新日期:2007-11-01 00:00:00

  • The role of aminopeptidases in haemoglobin degradation in Plasmodium falciparum-infected erythrocytes.

    abstract::Intra-erythrocytic Plasmodium parasites digest host cell haemoglobin and use the liberated amino acids for protein synthesis. Although several endoproteases (aspartic, cysteine, and metallo-) have been shown to be involved in the initial stages of haemoglobin degradation, little is known about the steps immediately be...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Gavigan CS,Dalton JP,Bell A

    更新日期:2001-09-28 00:00:00

  • Characterization of a Fasciola gigantica protein carrying two DM9 domains reveals cellular relocalization property.

    abstract::Even at the present age of whole-organism analysis, e.g., genomics, transcriptomics, and proteomics, the biological roles of many proteins remain unresolved. Classified among the proteins of unknown function is a family of proteins harboring repeats of the DM9 domain, a 60-75 amino acids motif first described in a sma...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Phadungsil W,Smooker PM,Vichasri-Grams S,Grams R

    更新日期:2016-01-01 00:00:00

  • Hepatic mixed-function oxidase activity in mice infected with Trypanosoma brucei gambiense or treated with trypanocides.

    abstract::Three days after mice were inoculated with Trypanosoma brucei gambiense, total hepatic cytochrome P-450 levels were decreased 14% from control values, while the specific mixed-function oxidase activity (per nmole of cytochrome P-450) was inhibited almost 40% in infected animals. Furthermore, drugs used to treat trypan...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Shertzer HG,Hall JE,Seed JR

    更新日期:1981-08-01 00:00:00

  • Dominant lethality and insect population control.

    abstract::Recent advances in insect genetic engineering have opened up new possibilities in the genetic control of insect vectors of human diseases. We outline the current state of the sterile insect technique and show how the use of engineered dominant lethals can greatly increase the effectiveness of this approach. We conside...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Alphey L,Andreasen M

    更新日期:2002-05-01 00:00:00

  • Differential toxicity of ricin and diphtheria toxin for bloodstream forms of Trypanosoma brucei.

    abstract::The cytotoxicity of ricin and diphtheria toxin was studied in culture-adapted bloodstream forms of Trypanosoma brucei. Although ricin is endocytosed at a rate comparable to that of other internalized macromolecules, it is nontoxic to bloodstream-form trypanosomes. The resistance lies partly in low susceptibility of th...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Scory S,Steverding D

    更新日期:1997-12-01 00:00:00

  • The two mechanisms for antigenic variation in Trypanosoma brucei are independent processes.

    abstract::Antigenic switching in Trypanosoma brucei can occur either by the production of a telomeric copy of a variant surface glycoprotein (VSG) gene through a gene conversion mechanism or by the nonduplicative activation of a telomeric VSG gene. The 5 VSG gene telomeric copy that is expressed in IsTaR 1 variant antigenic typ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Aline RF Jr,Stuart K

    更新日期:1985-06-01 00:00:00

  • PfCG2, a Plasmodium falciparum protein peripherally associated with the parasitophorous vacuolar membrane, is expressed in the period of maximum hemoglobin uptake and digestion by trophozoites.

    abstract::A Plasmodium falciparum gene closely linked to the chloroquine resistance locus encodes PfCG2, a predicted 320-330kDa protein. In the parasitized erythrocyte, PfCG2 expression rises sharply in the trophozoite stage and is detected in electron-dense patches along the parasitophorous vacuolar membrane (PVM), in the cyto...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Cooper RA,Papakrivos J,Lane KD,Fujioka H,Lingelbach K,Wellems TE

    更新日期:2005-12-01 00:00:00

  • DNA sequence of Crithidia fasciculata kinetoplast minicircles.

    abstract::Kinetoplast DNA (kDNA) networks of the insect trypanosomatid Crithidia fasciculata strain CF-C1 contain a nearly homogeneous population of kDNA minicircles as judged by restriction enzyme cleavage analysis. We have determined the entire nucleotide sequence of the major class of minicircles by analyzing M13 phage clone...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Sugisaki H,Ray DS

    更新日期:1987-04-01 00:00:00

  • The Leishmania promastigote surface antigen 2 complex is differentially expressed during the parasite life cycle.

    abstract::The promastigote surface antigen 2 (PSA-2) complex comprises a family of antigenically similar polypeptides of M(r) 96,000, 80,000 and 50,000, anchored to the membrane with glycosylphosphatidylinositol. Although PSA-2 was initially detected only in promastigotes, Northern blot analysis indicated that mRNA transcripts ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Handman E,Osborn AH,Symons F,van Driel R,Cappai R

    更新日期:1995-11-01 00:00:00

  • 3-Methyladenine blocks Toxoplasma gondii division prior to centrosome replication.

    abstract::The apicomplexan Toxoplasma gondii replicates by endodyogeny, in which replicated organelles assemble into nascent daughter buds within the maternal parasite. The mechanisms governing this complex sequence are not understood. We now report that the kinase inhibitor 3-methlyadenine (3-MA) efficiently blocks T. gondii r...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Wang Y,Karnataki A,Parsons M,Weiss LM,Orlofsky A

    更新日期:2010-10-01 00:00:00

  • The enzymes of pyrimidine biosynthesis in a range of parasitic protozoa and helminths.

    abstract::The activities of carbamoylphosphate synthase, aspartate transcarbamoylase, dihydroorotase, orotate phosphoribosyl transferase and orotidine-5'-phosphate decarboxylase, five of the six enzymes of pyrimidine biosynthesis, have been measured in Crithidia fasciculata, Trypanosoma cruzi, Leishmania major, Trichomonas vagi...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hill B,Kilsby J,Rogerson GW,McIntosh RT,Ginger CD

    更新日期:1981-02-01 00:00:00

  • Mosquito infectivity is directly related to the proportion of repetitive DNA in Plasmodium berghei.

    abstract::A strain of Plasmodium berghei (NK 65) was followed during syringe transmission in mice for over 120 passages after the last complete cycle, while the following parameters were monitored: (a) capacity to infect mosquitoes, inducing oocyst formation; (b) presence in the peripheral blood of morphologically identifiable ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Birago C,Bucci A,Dore E,Frontali C,Zenobi P

    更新日期:1982-07-01 00:00:00

  • Structural analysis of the acetaldehyde dehydrogenase activity of Entamoeba histolytica alcohol dehydrogenase 2 (EhADH2), a member of the ADHE enzyme family.

    abstract::The ADHE family of enzymes are bifunctional acetaldehyde dehydrogenase (ALDH)/alcohol dehydrogenase (ADH) enzymes that probably arose from the fusion of genes encoding separate ALDH and ADH enzymes. Here we have used the Entamoeba histolytica alcohol dehydrogenase 2 (EhADH2) enzyme as a prototype to analyze the struct...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Chen M,Li E,Stanley SL Jr

    更新日期:2004-10-01 00:00:00

  • Biochemical and molecular characterization of the pyrimidine biosynthetic enzyme dihydroorotate dehydrogenase from Toxoplasma gondii.

    abstract::The pyrimidine biosynthesis pathway in the protozoan pathogen Toxoplasma gondii is essential for parasite growth during infection. To investigate the properties of dihydroorotate dehydrogenase (TgDHOD), the fourth enzyme in the T. gondii pyrimidine pathway, we expressed and purified recombinant TgDHOD. TgDHOD exhibite...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hortua Triana MA,Huynh MH,Garavito MF,Fox BA,Bzik DJ,Carruthers VB,Löffler M,Zimmermann BH

    更新日期:2012-08-01 00:00:00

  • Molecular cloning of Trypanosoma brucei CK2 catalytic subunits: the alpha isoform is nucleolar and phosphorylates the nucleolar protein Nopp44/46.

    abstract::We have demonstrated previously that Nopp44/46, an abundant nucleolar phosphoprotein of Trypanosoma brucei, is associated with a protein kinase. In many organisms multiple nucleolar proteins are phosphorylated by the protein kinase CK2, formerly known as casein kinase II. Here we report the identification of two T. br...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Park JH,Brekken DL,Randall AC,Parsons M

    更新日期:2002-01-01 00:00:00

  • Enhanced Ca2+ uptake by mouse erythrocytes in malarial (Plasmodium berghei) infection.

    abstract::Erythrocytes from Plasmodium berghei-infected mice on incubation either in plasma or artificial isotonic media showed an increase in uptake of 45Ca2+ compared with erythrocytes from uninfected mice. Infected cells (55% parasitaemia) incubated in plasma from normal or infected mice gave uptake rates of 9.8 and 8.1 nmol...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Krungkrai J,Yuthavong Y

    更新日期:1983-03-01 00:00:00

  • Role of 3'-untranslated regions in the regulation of hexose transporter mRNAs in Trypanosoma brucei.

    abstract::Trypanosoma brucei is a unicellular parasite that is transmitted from one mammalian host to the next by tsetse flies. The expression of many trypanosome genes is regulated during the life cycle but there is no evidence for developmental control of transcription by RNA polymerase II. T. brucei expresses at least two he...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hotz HR,Lorenz P,Fischer R,Krieger S,Clayton C

    更新日期:1995-12-01 00:00:00

  • A diamidine-resistant Trypanosoma equiperdum clone contains a P2 purine transporter with reduced substrate affinity.

    abstract::Following the demonstration that the transport of melaminophenyl arsenical drugs in Trypanosoma brucei is dependent upon an unusual adenosine nucleoside transporter (Carter and Fairlamb, Nature 361 (1993) 173-175) we have investigated adenosine transport in the related parasite Trypanosoma equiperdum (Botat1.1) and a ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Barrett MP,Zhang ZQ,Denise H,Giroud C,Baltz T

    更新日期:1995-07-01 00:00:00

  • The reversible effect of glucose on the energy metabolism of Schistosoma mansoni cercariae and schistosomula.

    abstract::This study on isolated cercarial bodies demonstrates that the biological transformation from cercaria to schistosomulum and the biochemical transition from an aerobic to an anaerobic energy metabolism are separate processes, which are not necessarily linked. The metabolic transition depends on the external glucose con...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Horemans AM,Tielens AG,van den Bergh SG

    更新日期:1992-03-01 00:00:00

  • Real-time PCR for sexing Schistosoma mansoni cercariae.

    abstract::The gender of cercarial larvae can only be determined using molecular methods. End point PCR methods that amplify repetitive markers on the W chromosome of the female (ZW) parasites have been developed, but sometimes results are ambiguous or incorrect. To more effectively distinguish sexes, and to determine why end po...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Chevalier FD,Le Clec'h W,Alves de Mattos AC,LoVerde PT,Anderson TJ

    更新日期:2016-01-01 00:00:00

  • Transgenic Plasmodium berghei sporozoites expressing beta-galactosidase for quantification of sporozoite transmission.

    abstract::Malaria transmission occurs during a blood-meal of an infected Anopheles mosquito. Visualization and quantification of sporozoites along the journey from the mosquito midgut, where they develop, to the vertebrate liver, their final target organ, is important for understanding many aspects of sporozoite biology. Here w...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Engelmann S,Sinnis P,Matuschewski K

    更新日期:2006-03-01 00:00:00

  • An evaluation of different methods for labelling the surface of the filarial nematode Brugia pahangi with 125iodine.

    abstract::The specificity of a range of 125I labelling techniques (Chloramine T, Iodogen, Bolton and Hunter reagent, lactoperoxidase and iodosulfanilic acid) to the surface of the filarial nematode Brugia pahangi was evaluated by autoradiography of sections of labelled worms and of dried SDS-polyacrylamide gels following electr...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Marshall E,Howells RE

    更新日期:1985-06-01 00:00:00

  • Uptake of the trypanocidal drug suramin by bloodstream forms of Trypanosoma brucei and its effect on respiration and growth rate in vivo.

    abstract::After a single intravenous injection of suramin the rate of removal of the drug from the plasma into other tissue compartments of the rat is independent of initial concentration. The data can be fitted to the sum of two exponential functions, consistent with a two-compartment, open model system. Trypanosomes take up o...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Fairlamb AH,Bowman IB

    更新日期:1980-10-01 00:00:00

  • Expression profiling of the Leishmania life cycle: cDNA arrays identify developmentally regulated genes present but not annotated in the genome.

    abstract::As genomic sequencing of Leishmania nears completion, functional analyses that provide a global genetic perspective on biological processes are important. Despite polycistronic transcription, RNA transcript abundance can be measured using microarrays. To provide a resource to evaluate cDNA arrays, we undertook 5' expr...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Almeida R,Gilmartin BJ,McCann SH,Norrish A,Ivens AC,Lawson D,Levick MP,Smith DF,Dyall SD,Vetrie D,Freeman TC,Coulson RM,Sampaio I,Schneider H,Blackwell JM

    更新日期:2004-07-01 00:00:00

  • L-myo-Inositol 1-phosphate synthase from Entamoeba histolytica.

    abstract::L-myo-Inositol 1-phosphate synthase (I-1-P synthase) catalyses the primary reaction for the synthesis of inositol in a variety of prokaryotes, eukaryotes and in the chloroplasts of algae and higher plants. Inositol is a precursor of essential macromolecules like membrane phospholipids, GPI anchor proteins and lipophos...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Lohia A,Hait NC,Majumder AL

    更新日期:1999-01-05 00:00:00

  • The Plasmodium falciparum exported protein PF3D7_0402000 binds to erythrocyte ankyrin and band 4.1.

    abstract::Plasmodium falciparum extensively modifies the infected red blood cell (RBC), resulting in changes in deformability, shape and surface properties. These alterations suggest that the RBC cytoskeleton is a major target for modification during infection. However, the molecular mechanisms leading to these changes are larg...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Shakya B,Penn WD,Nakayasu ES,LaCount DJ

    更新日期:2017-09-01 00:00:00

  • Microsatellite polymorphism in the sexually transmitted human pathogen Trichomonas vaginalis indicates a genetically diverse parasite.

    abstract::Given the growing appreciation of serious health sequelae from widespread Trichomonas vaginalis infection, new tools are needed to study the parasite's genetic diversity. To this end we have identified and characterized a panel of 21 microsatellites and six single-copy genes from the T. vaginalis genome, using seven l...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Conrad M,Zubacova Z,Dunn LA,Upcroft J,Sullivan SA,Tachezy J,Carlton JM

    更新日期:2011-01-01 00:00:00

  • Profiling the malaria genome: a gene survey of three species of malaria parasite with comparison to other apicomplexan species.

    abstract::We have undertaken the first comparative pilot gene discovery analysis of approximately 25,000 random genomic and expressed sequence tags (ESTs) from three species of Plasmodium, the infectious agent that causes malaria. A total of 5482 genome survey sequences (GSSs) and 5582 ESTs were generated from mung bean nucleas...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Carlton JM,Muller R,Yowell CA,Fluegge MR,Sturrock KA,Pritt JR,Vargas-Serrato E,Galinski MR,Barnwell JW,Mulder N,Kanapin A,Cawley SE,Hide WA,Dame JB

    更新日期:2001-12-01 00:00:00

  • Primary structure of Trypanosoma cruzi small-subunit ribosomal RNA coding region: comparison with other trypanosomatids.

    abstract::A Trypanosoma cruzi small subunit ribosomal RNA gene was sequenced from genomic recombinant plasmid clones. The assigned coding region was 2319 bp, the longest SSU rRNA gene described to date. On the basis of comparisons with published sequences from Crithidia fasciculata, Trypanosoma brucei, and Leishmania donovani, ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hernández R,Rios P,Valdés AM,Piñero D

    更新日期:1990-06-01 00:00:00