Identification of Eimeria bovis merozoite cDNAs using differential mRNA display.


:Differences in gene expression between Eimeria bovis sporozoites and first-generation merozoites were analyzed using the technique of differential mRNA display. Approx. 5% of the sequences detected in first-generation merozoites appear to be unique relative to sporozoites. Several of the bands corresponding to merozoite-specific gene expression were isolated and cloned. Northern blot analysis revealed that the cDNA fragments DMZ-7, DMZ-8 and NMZ-6 hybridized to mRNAs expressed at > 50-fold higher levels in merozoites relative to sporozoites. A fourth cDNA fragment, NMZ-4, hybridized to a mRNA expressed at 3-fold higher levels in merozoites. Further characterization demonstrated that expression of DMZ-8 in E. bovis-infected bovine cells begins as early as 12 h after sporozoite invasion and continues throughout the entire 14 days of first-generation schizogony. Sequence analysis of each of the four merozoite cDNAs failed to identify any significant similarity to any entries in the GenBank database, suggesting that these developmentally regulated genes may be unique to coccidian parasites.


Mol Biochem Parasitol


Abrahamsen MS,Johnson RR,Hathaway M,White MW




Has Abstract


1995-05-01 00:00:00














  • Design of Onchocerca DNA probes based upon analysis of a repeated sequence family.

    abstract::Repeated DNA sequences have been instrumental in the development of DNA probes for many different parasites. Isolation of such DNA probes has generally been accomplished by differential screening of genomic libraries with total genomic DNA preparations. In the current work, a rational design strategy is presented for ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Zimmerman PA,Toe L,Unnasch TR

    更新日期:1993-04-01 00:00:00

  • Mechanisms of respiration and phosphorylation in Ascaris muscle mitochondria.

    abstract::In Ascaris muscle mitochondria the major respiratory chain-linked phosphorylation activity is accomplished by a NADH-linked reduction of fumarate to succinate. Oxygen can also be employed as a terminal electron acceptor via a cyanide- and salicyl-hydroxamate-resistant terminal oxidase. As in fumarate-dependent electro...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Köhler P,Bachmann R

    更新日期:1980-04-01 00:00:00

  • Heat shock and stress response in Giardia lamblia.

    abstract::The heat shock response was studied as a model for control of gene expression and protein synthesis in Giardia lamblia. Cultured trophozoites were metabolically labelled with [35S]methionine, and proteins were analysed by SDS-polyacrylamide gel electrophoresis and fluorography. A temperature shift from 37 degrees C to...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Lindley TA,Chakraborty PR,Edlind TD

    更新日期:1988-03-01 00:00:00

  • Antisense RNA and RNAi in protozoan parasites: working hard or hardly working?

    abstract::The complex life cycles of many protozoan parasites require the ability to respond to environmental and developmental cues through regulated gene expression. Traditionally, parasitologists have investigated these mechanisms by identifying and characterizing proteins that are necessary for the regulated expression of t...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Militello KT,Refour P,Comeaux CA,Duraisingh MT

    更新日期:2008-02-01 00:00:00

  • A novel rhoptry protein in Toxoplasma gondii bradyzoites and merozoites.

    abstract::The secretory organelles of Toxoplasma gondii orchestrate invasion of the host cell and establish the parasitophorous vacuole. Although much has been learned about the roles played by these organelles in invasion by the tachyzoite stage, little is known about the contents or functions of these organelles during bradyz...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Schwarz JA,Fouts AE,Cummings CA,Ferguson DJ,Boothroyd JC

    更新日期:2005-12-01 00:00:00

  • Base J glucosyltransferase does not regulate the sequence specificity of J synthesis in trypanosomatid telomeric DNA.

    abstract::Telomeric DNA of trypanosomatids possesses a modified thymine base, called base J, that is synthesized in a two-step process; the base is hydroxylated by a thymidine hydroxylase forming hydroxymethyluracil (hmU) and a glucose moiety is then attached by the J-associated glucosyltransferase (JGT). To examine the importa...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Bullard W,Cliffe L,Wang P,Wang Y,Sabatini R

    更新日期:2015-12-01 00:00:00

  • Food vacuole plasmepsins are processed at a conserved site by an acidic convertase activity in Plasmodium falciparum.

    abstract::Intraerythrocytic Plasmodium falciparum digests vast amounts of hemoglobin within an acidic food vacuole (FV). Four homologous aspartic proteases participate in hemoglobin degradation within the FV. Plasmepsin (PM) I and II are thought to initiate degradation of the native hemoglobin molecule. PM IV and histo-aspartic...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Banerjee R,Francis SE,Goldberg DE

    更新日期:2003-07-01 00:00:00

  • Proteolytic activity of Plasmodium falciparum subtilisin-like protease 3 on parasite profilin, a multifunctional protein.

    abstract::Subtilisin-like proteases of malaria parasite Plasmodium falciparum (PfSUB1, 2 and 3) are expressed at late asexual blood stages. PfSUB1 and 2 are considered important drug targets due to their essentiality for parasite blood stages and role in merozoite egress and invasion of erythrocytes. We have earlier shown the i...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Alam A,Bhatnagar RK,Relan U,Mukherjee P,Chauhan VS

    更新日期:2013-10-01 00:00:00

  • Characterisation of protein kinase C like activities in Trypanosoma brucei.

    abstract::Protein kinase activities in bloodstream and procyclic forms of Trypanosoma brucei have been partially purified and characterised. Cytosolic extracts were separated on DEAE-cellulose and assayed for the ability to phosphorylate histone in the presence of Ca2+ and diacylglycerol. Five peaks of activity were identified ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Keith K,Hide G,Tait A

    更新日期:1990-11-01 00:00:00

  • The Leishmania mexicana proteasome.

    abstract::As a start to understanding the importance of intracellular proteolysis in the protozoon Leishmania mexicana, the parasite proteasome has been purified and characterised. The L. mexicana proteasome is similar to proteasomes from other eukaryotes. It is soluble, and the 20S form has a mass of around 670 kDa, composed o...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Robertson CD

    更新日期:1999-09-20 00:00:00

  • Molecular characterisation and localisation of an Onchocerca volvulus pi-class glutathione S-transferase.

    abstract::Glutathione S-transferases (GSTs) constitute a major detoxification mechanism in helminth organisms and are regarded vaccine candidates against helminth infections. Onchocerca volvulus glutathione-binding proteins were purified from the aqueous soluble fraction of homogenised adult females by affinity chromatography o...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Salinas G,Braun G,Taylor DW

    更新日期:1994-07-01 00:00:00

  • Cytochrome c and c1 heme lyases are essential in Plasmodium berghei.

    abstract::Malaria parasites possess a de novo heme synthetic pathway. Interestingly, this pathway is dispensable during the blood stages of development in mammalian hosts. The assembly of the two most important hemeproteins, cytochromes c and c1, is mediated by cytochrome heme lyase enzymes. Plasmodium spp. possess two cytochro...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Posayapisit N,Songsungthong W,Koonyosying P,Falade MO,Uthaipibull C,Yuthavong Y,Shaw PJ,Kamchonwongpaisan S

    更新日期:2016-01-01 00:00:00

  • Characterization of the phytochelatin synthase from the human parasitic nematode Ancylostoma ceylanicum.

    abstract::Hookworm disease is a debilitating worm infection that affects hundreds of millions of people. Despite the existence of anthelmintic drugs, reports have testified of a decrease in efficacy of these drugs. Therefore, it is imperative to find new drugs and drug targets for hookworm disease treatment. In this study we id...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Rigouin C,Vermeire JJ,Nylin E,Williams DL

    更新日期:2013-09-01 00:00:00

  • Identification and characterization of an Onchocerca volvulus cDNA clone encoding a highly immunogenic calponin-like protein.

    abstract::The identification and characterization of a recombinant cDNA clone, designated OV9M, expressing an antigen present in Onchocerca volvulus infective larvae and adult stages is described. Clone OV9M was identified by screening a lambda gt11 cDNA expression library derived from adult O. volvulus mRNA using pooled rabbit...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Irvine M,Huima T,Prince AM,Lustigman S

    更新日期:1994-05-01 00:00:00

  • Identification and localization of a Novel Invasin of Plasmodium falciparum.

    abstract::Plasmodium falciparum is the causative organism for the most severe form of malaria among humans. The clinical symptoms are accredited to the asexual stage of parasite life cycle, involving merozoite invasion of erythrocyte, development and re-invasion into the new erythrocyte. Interaction of parasite proteins present...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hans N,Relan U,Dubey N,Gaur D,Chauhan VS

    更新日期:2015-08-01 00:00:00

  • Pyrazolopyrimidine metabolism in African trypanosomes: metabolic similarities to Trypanosoma cruzi and Leishmania spp.

    abstract::Growth inhibition and radioisotope incorporation studies with allopurinol (4-hydroxypyrazolo(3,4-d)pyrimidine) have shown that the African trypanosomes are biologically and biochemically similar to Leishmania spp. and Trypanosoma cruzi with respect to their response to this compound. These organisms, as a group, share...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Berens RL,Marr JJ,Brun R

    更新日期:1980-04-01 00:00:00

  • Arp1, an actin-related protein, in Plasmodium berghei.

    abstract::Actin-related proteins (Arps) constitute a family of eukaryotic cytoskeletal proteins involved in such diverse events as cell motility, cytokinesis, vesicle transport, and chromatin remodelling. Previously, in a study of Plasmodium berghei gene expression in ookinetes and oocysts, we detected stage-specific increased ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Siden-Kiamos I,Schüler H,Liakopoulos D,Louis C

    更新日期:2010-10-01 00:00:00

  • Regulation of hsp70 expression in Trypanosoma cruzi by temperature and growth phase.

    abstract::The steady-state level of the hsp70 mRNAs of Trypanosoma cruzi cultured at different temperatures and growth conditions has been analyzed by Northern blotting. We show that only one size class of hsp70 mRNA, of about 2.2 kb, is transcribed from the hsp70 cluster and that its transcription is constitutive at 28 degrees...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Requena JM,Jimenez-Ruiz A,Soto M,Assiego R,Santarén JF,Lopez MC,Patarroyo ME,Alonso C

    更新日期:1992-07-01 00:00:00

  • Antigenic variation and cytoadhesion in Babesia bovis and Plasmodium falciparum: different logics achieve the same goal.

    abstract::Babesia bovis is a protozoal hemoparasite of cattle which behaves in certain crucial respects like Plasmodium falciparum, despite being phylogenetically distant and having many differences in its life cycle. The shared behavioral attributes of rapid antigenic variation and cytoadhesion/sequestration are thought to con...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Allred DR,Al-Khedery B

    更新日期:2004-03-01 00:00:00

  • The T687G SNP in a P-glycoprotein gene of Fasciola hepatica is not associated with resistance to triclabendazole in two resistant Australian populations.

    abstract::Triclabendazole (TCBZ) is widely used for control of Fasciola hepatica (liver fluke) in animals and humans and resistance to this drug is now widespread. However, the mechanism of resistance to TCBZ is not known. A T687G single nucleotide polymorphism (SNP) in a P-glycoprotein gene was proposed as a molecular marker f...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Elliott TP,Spithill TW

    更新日期:2014-11-01 00:00:00

  • Characterization and detection of plant trypanosomatids by sequence analysis of the small subunit ribosomal RNA gene.

    abstract::The complete sequences of the genomic small subunit ribosomal RNA gene from two Phytomonas isolates: one associated with palm pathologies (P. cocos FGuiana) and one found in lactiferous plants with no apparent pathology (P. Euphorbe Senegal), were analyzed. Partial sequences from a number of other Phytomonas isolates ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Marché S,Roth C,Philippe H,Dollet M,Baltz T

    更新日期:1995-04-01 00:00:00

  • cDNA expressed sequence tags of Trypanosoma brucei rhodesiense provide new insights into the biology of the parasite.

    abstract::A total of 518 expressed sequence tags (ESTs) have been generated from clones randomly selected from a cDNA library and a spliced leader sub-library of a Trypanosoma brucei rhodesiense bloodstream clone. 205 (39%) of the clones were identified based on matches to 113 unique genes in the public databases. Of these, 71 ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: el-Sayed NM,Alarcon CM,Beck JC,Sheffield VC,Donelson JE

    更新日期:1995-07-01 00:00:00

  • Substrate specificity of recombinant cysteine proteinase, CPB, of Leishmania mexicana.

    abstract::The primary S(1) subsite specificity of a recombinant cysteine proteinase, CPB2.8 Delta CTE, of Leishmania mexicana was investigated in a systematic way using a series of peptides derived from Abz-KLRFSKQ-EDDnp in which Arg was substituted by all natural amino acids (where Abz is ortho-amino-benzoyl and EDDnp is N-[2,...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Alves LC,Judice WA,St Hilaire PM,Meldal M,Sanderson SJ,Mottram JC,Coombs GH,Juliano L,Juliano MA

    更新日期:2001-08-01 00:00:00

  • Chemical modification of fructose bisphosphate aldolase from Trypanosoma brucei compared to aldolase from rabbit muscle and Staphylococcus aureus.

    abstract::Chemical modifications of Class I aldolases from Trypanosoma brucei, rabbit muscle and Staphylococcus aureus with carboxypeptidase A, glyceraldehyde 3-phosphate and cysteine-specific reagents revealed the following differences between the three homologous enzymes. Aldolase from S. aureus was not affected by any of the...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Callens M,Opperdoes FR

    更新日期:1991-07-01 00:00:00

  • A Plasmodium multi-domain protein possesses multiple inositol phosphate kinase activities.

    abstract::The synchronization of intraerythrocytic maturation of Plasmodium parasites is an important factor in the malaria infection process. Synchronization is mediated by inositol phosphate (InsP(x))-induced Ca(2+)-release from internal stores. To further investigate the InsP(x) metabolism in these parasites a Plasmodium pro...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Stritzke C,Nalaskowski MM,Fanick W,Lin H,Mayr GW

    更新日期:2012-12-01 00:00:00

  • Control of VSG gene expression sites.

    abstract::Trypanosoma brucei survives in mammals by antigenic variation of its surface coat consisting of variant surface glycoprotein (VSG). Trypanosomes change coat mainly by replacing the transcribed VSG genes in an active telomeric expression site by a different VSG gene. There are about 20 different expression sites and tr...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Borst P,Ulbert S

    更新日期:2001-04-25 00:00:00

  • Repeat structures in a Plasmodium falciparum protein (MESA) that binds human erythrocyte protein 4.1.

    abstract::The mature-parasite-infected erythrocyte surface antigen (MESA, also known as PfEMP-2 and pp300) of Plasmodium falciparum is a phosphoprotein of approx. 250-300 kDa that is exported from the parasite to the erythrocyte membrane skeleton where it binds to protein 4.1. Determination of the primary sequence of MESA revea...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Coppel RL

    更新日期:1992-02-01 00:00:00

  • Proteomic analysis of Schistosoma mansoni egg secretions.

    abstract::Schistosomiasis remains a largely neglected, global health problem. The morbid pathology of the disease stems from the host's inflammatory response to parasite eggs trapped in host tissues. Long term host/parasite survival is dependent upon the successful modulation of the acute pathological response, which is induced...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Cass CL,Johnson JR,Califf LL,Xu T,Hernandez HJ,Stadecker MJ,Yates JR 3rd,Williams DL

    更新日期:2007-10-01 00:00:00

  • Mitochondrial genomes of Meloidogyne chitwoodi and M. incognita (Nematoda: Tylenchina): comparative analysis, gene order and phylogenetic relationships with other nematodes.

    abstract::Root-knot nematodes (Meloidogyne spp.) are among the most important plant pathogens. In this study, the mitochondrial (mt) genomes of the root-knot nematodes, M. chitwoodi and M. incognita were sequenced. PCR analyses suggest that both mt genomes are circular, with an estimated size of 19.7 and 18.6-19.1kb, respective...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Humphreys-Pereira DA,Elling AA

    更新日期:2014-03-01 00:00:00

  • Role of glutathione in the detoxification of ferriprotoporphyrin IX in chloroquine resistant Plasmodium berghei.

    abstract::The reduction in hemozoin content is a well known feature of chloroquine-resistant Plasmodium berghei. Using NK65-derived lines displaying increasing resistance levels, we observed an inverse relationship between the hemozoin content, and the glutathione (GSH) and glutathione S-transferase (GST) levels. Treatment of h...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Platel DF,Mangou F,Tribouley-Duret J

    更新日期:1999-01-25 00:00:00