Cloning and characterization of the ribosomal RNA gene repeat from Ostertagia ostertagi.


:Clones of the rRNA genes of Ostertagia ostertagi were selected from a library prepared from the genomic DNA of adult worms of strain LA-2. A 13.4-kb insert in a clone, lambda OOR78, is comprised of one complete 7.5-kb rDNA unit and portions of two adjacent units. The rDNA unit is directly repeated in a head-to-tail fashion and represents approximately 0.9% of the total genomic DNA. This repeating unit appears to be the only long tandemly repeated sequence in the genome. Restriction enzyme recognition sites in the rDNAs of four strains of O. ostertagi were fully conserved with the exception of one PstI site present in the large rRNA gene which was absent from a proportion of the genes of the LA-2 strain. The rDNA of O. ostertagi is more similar to that of Caenorhabditis elegans in unit length and arrangement than to parasitic helminths previously examined.


Mol Biochem Parasitol


Dame JB,Yowell CA,Courtney CH,Lindgren WG




Has Abstract


1991-04-01 00:00:00














  • Purification, characterization, and immunochemical studies of beta-N-acetyl-D-hexosaminidase from the parasitic nematode Trichinella spiralis.

    abstract::The exoglycosidase, beta-N-acetyl-D-hexosaminidase was purified 600-fold from the muscle-stage larvae (L1) of Trichinella spiralis. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), the purified enzyme-active fraction contained 4 polypeptides with apparent molecular weights of 100,000, 68,000, 5...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Rhoads ML

    更新日期:1988-10-01 00:00:00

  • Mosquito innate immunity: involvement of beta 1,3-glucan recognition protein in melanotic encapsulation immune responses in Armigeres subalbatus.

    abstract::Beta 1,3-glucan recognition proteins (GRP) have specific affinity for beta 1,3-glucan, a component on the surface of fungi and bacteria. By interacting with beta 1,3-glucan, GRP initiates activation of prophenoloxidase, a key enzyme in the signaling pathway leading to melanotic encapsulation in invertebrates. In this ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Wang X,Fuchs JF,Infanger LC,Rocheleau TA,Hillyer JF,Chen CC,Christensen BM

    更新日期:2005-01-01 00:00:00

  • Conservation of the Plasmodium falciparum sporozoite surface protein gene, STARP, in field isolates and distinct species of Plasmodium.

    abstract::The extent of structural conservation of the Plasmodium falciparum sporozoite surface protein gene, STARP, recently characterized in the T9/96 clone, has been analyzed using the polymerase chain reaction. Results from Ivory Coast and Thai clones, field isolates originating from Brazil and Kenya and laboratory-maintain...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Fidock DA,Sallenave-Sales S,Sherwood JA,Gachihi GS,Ferreira-da-Cruz MF,Thomas AW,Druilhe P

    更新日期:1994-10-01 00:00:00

  • Extensive homologies between Leishmania donovani chromosomes of markedly different size.

    abstract::The smallest chromosome (230 kb) of the HU3 stock of Leishmania donovani was purified from an orthogonal field alternation (OFAGE) gel, digested with PstI and cloned into the plasmid pUC13. When used to probe Southern blots of OFAGE gels, the cloned sequences recognised one or more large chromosomes in all L. donovani...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Bishop RP

    更新日期:1990-01-01 00:00:00

  • Zinc finger nuclease technology: A stable tool for high efficiency transformation in bloodstream form T. brucei.

    abstract::In Trypanosoma brucei, the generation of knockout mutants is relatively easy compared to other organisms as transfection methods are well established. These methods have their limitations, however, when it comes to the generation of genome-wide libraries that require a minimum of several hundred thousand transformants...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Schumann G,Kangussu-Marcolino MM,Doiron N,Käser S,de Assis Burle-Caldas G,DaRocha WD,Teixeira SM,Roditi I

    更新日期:2017-04-01 00:00:00

  • Immunoprecipitation of surface antigen precursors from Schistosoma mansoni messenger RNA in vitro translation products.

    abstract::Messenger RNA has been isolated from adults and eggs of Schistosoma mansoni and translated in vitro in mRNA dependent rabbit reticulocyte lysate. The in vitro translation products have been immunoprecipitated using a wide variety of hyperimmune and infection sera from rabbits, mice and humans. A large number of in vit...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Taylor DW,Cordingley JS,Butterworth AE

    更新日期:1984-03-01 00:00:00

  • Isolation and characterization of infective and non-infective clones of Leishmania tropica.

    abstract::Cloning by limit dilution of an isolate of Leishmania tropica (LRC-L137) that is infective for mice resulted in 7 stable clones, only one of which was infective in BALB/c mice. Three of the non-infective clones that were examined for survival in BALB/c macrophages in vitro seemed to be killed more readily, suggesting ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Handman E,Hocking RE,Mitchell GF,Spithill TW

    更新日期:1983-02-01 00:00:00

  • Effects of oxygen concentration on the intermediary metabolism of Leishmania major promastigotes.

    abstract::Leishmania major promastigotes grown in late log phase were incubated with glucose as sole exogenous carbon source in the presence of 5% CO2 and the amounts of glucose consumed and of the major products formed--succinate, pyruvate, alanine, acetate, glycerol, and D-lactate--were measured as a function of pO2. Glucose ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Keegan F,Blum JJ

    更新日期:1990-03-01 00:00:00

  • Serological cross-reaction between high molecular weight proteins synthesized in blood schizonts of Plasmodium yoelii, Plasmodium chabaudi and Plasmodium falciparum.

    abstract::A 230 000 molecular weight (MW) Plasmodium yoelii protein, a 250 000 MW P. chabaudi protein and a 195 000 MW P. falciparum protein, identified using monoclonal antibodies, have similar characteristics, and have been implicated as protective antigens. In this study the serological relationship between these proteins wa...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Holder AA,Freeman RR,Newbold CI

    更新日期:1983-11-01 00:00:00

  • A developmentally regulated cysteine protease gene family in Haemonchus contortus.

    abstract::The nucleotide sequence of a gene encoding a 35-kDa thiol protease of the parasitic nematode Haemonchus contortus has been determined. The gene, designated AC-2, shares 97% nucleotide sequence identity and 98% amino acid identity with previously characterized AC-1 cDNAs encoding the thiol protease. The AC-2 gene spans...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Pratt D,Cox GN,Milhausen MJ,Boisvenue RJ

    更新日期:1990-12-01 00:00:00

  • Sm22.6 antigen is an inhibitor to human thrombin.

    abstract::The physiological role of Schistosomiasis mansoni 22.6 antigen (sm22.6 Ag) and its pathogenic effect on the human host has never been reported. Recombinant sm22.6 Ag is a homogenous polymer under non-denaturing/non-reducing conditions, and an inhibitor to human thrombin. Kinetic and Western blot assays show that the r...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Lin YL,He S

    更新日期:2006-05-01 00:00:00

  • Transient expression of luciferase in Entamoeba histolytica driven by the ferredoxin gene 5' and 3' regions.

    abstract::We have successfully transfected Entamoeba histolytica trophozoites with constructs containing ferredoxin sequences fused to the reporter gene luciferase. We have determined the conditions and parameters necessary to maximise transient luciferase expression in our system. Our optimal construct gave values of 268 x 10(...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Gilchrist CA,Streets HL,Ackers JP,Hall R

    更新日期:1995-10-01 00:00:00

  • Molecular biology of the hexokinase isoenzyme pattern that distinguishes pathogenic Entamoeba histolytica from nonpathogenic Entamoeba dispar.

    abstract::The electrophoretic patterns of hexokinase and phosphoglucomutase have been widely used to distinguish Entamoeba histolytica from Entamoeba dispar isolates. Although E. histolytica and E. dispar, previously called pathogenic and nonpathogenic Entamoeba histolytica, differ clearly in sequences of many homologous genes,...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ortner S,Clark CG,Binder M,Scheiner O,Wiedermann G,Duchêne M

    更新日期:1997-05-01 00:00:00

  • Benznidazole-resistance in Trypanosoma cruzi: evidence that distinct mechanisms can act in concert.

    abstract::Benznidazole is the main drug used to treat Trypanosoma cruzi infections. However, frequent instances of treatment failure have been reported. To better understand potential resistance mechanisms, we analysed three clones isolated from a single parasite population that had undergone benznidazole-selection. These clone...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Campos MC,Leon LL,Taylor MC,Kelly JM

    更新日期:2014-01-01 00:00:00

  • Acute and persistent infection by a transfected Mo7 strain of Babesia bovis.

    abstract::A Mo7-derived Babesia bovis line stably transfected with the gfp-bsd gene was inoculated into two four to five months old calves, while two additional calves were inoculated with Mo7 parasites. Similar mild clinical signs were detected in all calves. B. bovis rap-1 was identified in the bloodstream by PCR four days po...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Suarez CE,Laughery JM,Schneider DA,Sondgeroth KS,McElwain TF

    更新日期:2012-09-01 00:00:00

  • Cloning and functional identification of a novel annexin subfamily in Cysticercus cellulosae.

    abstract::A novel cDNA was isolated from a library of Cysticercus cellulosae by immunological screening. Sequence analysis reveals that it has typical annexin structures. However, since its N-terminus is distinct and the core domain only has 32-44% amino acid identity to the tetrads of other annexins, the cDNA encodes a member ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hongli Y,Shuhan S,Ruiwen C,Yingjun G

    更新日期:2002-01-01 00:00:00

  • Nuclear actin-related protein is required for chromosome segregation in Toxoplasma gondii.

    abstract::Apicomplexa parasites use complex cell cycles to replicate that are not well understood mechanistically. We have established a robust forward genetic strategy to identify the essential components of parasite cell division. Here we describe a novel temperature sensitive Toxoplasma strain, mutant 13-20C2, which growth a...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Suvorova ES,Lehmann MM,Kratzer S,White MW

    更新日期:2012-01-01 00:00:00

  • Sequence homology and absence of mRNA defines a possible pseudogene member of the Trypanosoma cruzi gp85/sialidase multigene family.

    abstract::A genomic clone, pTt21, containing DNA apparently transcribed specifically in Trypanosoma cruzi trypomastigotes, was obtained by differentially screening a genomic library with trypomastigote and epimastigote cDNA. This 3444-bp clone contained open reading frames at each end, separated by a 1.8-kb non-coding region. T...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Takle GB,O'Connor J,Young AJ,Cross GA

    更新日期:1992-11-01 00:00:00

  • Identification of six Trypanosoma cruzi lineages by sequence-characterised amplified region markers.

    abstract::Six discrete phylogenetic lineages were recently identified in Trypanosoma cruzi, on the basis of multilocus enzyme electrophoresis and random amplified polymorphic DNA (RAPD) characterisation. The objective of the present study was to develop specific PCR-based markers for the identification of each of the six lineag...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Brisse S,Dujardin JC,Tibayrenc M

    更新日期:2000-11-01 00:00:00

  • The mitochondrial phosphate carrier TbMCP11 is essential for mitochondrial function in the procyclic form of Trypanosoma brucei.

    abstract::Conserved amongst all eukaryotes is a family of mitochondrial carrier proteins (SLC25A) responsible for the import of various solutes across the inner mitochondrial membrane. We previously reported that the human parasite Trypanosoma brucei possesses 26 SLC25A proteins (TbMCPs) amongst which two, TbMCP11 and TbMCP8, w...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Gao F,Voncken F,Colasante C

    更新日期:2020-05-01 00:00:00

  • Molecular characterisation of mitochondrial and cytosolic trypanothione-dependent tryparedoxin peroxidases in Trypanosoma brucei.

    abstract::In trypanosomatids, removal of hydrogen peroxide and other aryl and alkyl peroxides is achieved by the NADPH-dependent trypanothione peroxidase system, whose components are trypanothione reductase (TRYR), trypanothione, tryparedoxin (TRYX) and tryparedoxin peroxidase (TRYP). Here, we report the cloning of a multi-copy...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Tetaud E,Giroud C,Prescott AR,Parkin DW,Baltz D,Biteau N,Baltz T,Fairlamb AH

    更新日期:2001-09-03 00:00:00

  • In vitro selection of halofantrine resistance in Plasmodium falciparum is not associated with increased expression of Pgh1.

    abstract::Recent investigations into quinoline and phenanthrene methanol resistance in Plasmodium falciparum have described a linkage between amplification of the mdr homologue pfmdr1 and decreased susceptibility to mefloquine (MQ) and halofantrine (HF). We have examined the current theories on cross-resistance patterns and pfm...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ritchie GY,Mungthin M,Green JE,Bray PG,Hawley SR,Ward SA

    更新日期:1996-12-02 00:00:00

  • Molecular cloning and analysis of Ancylostoma ceylanicum glutamate-cysteine ligase.

    abstract::Glutamate-cysteine ligase (GCL) is a heterodimer enzyme composed of a catalytic subunit (GCLC) and a modifier subunit (GCLM). This enzyme catalyses the synthesis of γ-glutamylcysteine, a precursor of glutathione. cDNAs of the putative glutamate-cysteine ligase catalytic (Ace-GCLC) and modifier subunits (Ace-GCLM) of A...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Wiśniewski M,Lapiński M,Zdziarska A,Długosz E,Bąska P

    更新日期:2014-08-01 00:00:00

  • Structural studies on Plasmodium vivax merozoite surface protein-1.

    abstract::Plasmodium vivax infection is the second most common cause of malaria throughout the world. Like other Plasmodium species, P. vivax has a large protein complex, MSP-1, located on the merozoite surface. The C-terminal MSP-1 sub-unit, MSP-1(42), is cleaved during red blood cell invasion, causing the majority of the comp...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Babon JJ,Morgan WD,Kelly G,Eccleston JF,Feeney J,Holder AA

    更新日期:2007-05-01 00:00:00

  • Expression of foreign proteins in Trypanosoma congolense.

    abstract::An expression vector was constructed to express foreign genes in Trypanosoma congolense. The foreign gene and a neomycin phosphotransferase (NPT) gene are flanked by glutamate and alanine rich protein (GARP) gene processing signals and their expression is driven by a ribosomal RNA gene promoter. The plasmid is not mai...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Downey N,Donelson JE

    更新日期:1999-10-25 00:00:00

  • The subcellular localisation of trypanosome RRP6 and its association with the exosome.

    abstract::The exosome, a complex of 3'-exoribonucleases and associated proteins, is involved in the degradation of eukaryotic mRNAs in the cytoplasm, and has RNA processing and quality control functions in the nucleus. In yeast, the nuclear exosome differs from the cytoplasmic one in that it contains an additional non-essential...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Haile S,Cristodero M,Clayton C,Estévez AM

    更新日期:2007-01-01 00:00:00

  • Three acetylcholinesterases of the pinewood nematode, Bursaphelenchus xylophilus: insights into distinct physiological functions.

    abstract::Acetylcholinesterase (AChE) plays a key role in postsynaptic transmission in most animals. Nematodes encode multiple AChEs, implying its functional diversity. To explore physiological functions of multiple AChEs, three distinct AChEs (BxACE-1, BxACE-2, and BxACE-3) were identified and characterized from the pinewood n...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Kang JS,Lee DW,Choi JY,Je YH,Koh YH,Lee SH

    更新日期:2011-02-01 00:00:00

  • A bioinformatic analysis of the RAB genes of Trypanosoma brucei.

    abstract::RAB proteins are small GTPases with vital roles in eukaryotic intracellular transport; orthologous RABs appear to fulfil similar functions in diverse organisms. Trypanosoma brucei spp., the causative organisms of Old World trypanosomiasis of humans and domestic animals, have extremely effective endocytic and exocytic ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ackers JP,Dhir V,Field MC

    更新日期:2005-05-01 00:00:00

  • Trypanosoma brucei: the effect of glycerol on the anaerobic metabolism of glucose.

    abstract::Studies measuring the glycolytic intermediate and adenine nucleotide concentrations in Trypanosoma brucei metabolising glucose either aerobically or under conditions where glycerol-3-phosphate oxidase is inactive have shown the following: 1. Inhibition with 0.5 mM salicylhydroxamic acid (SHAM) accurately simulates ana...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hammond DJ,Bowman IB

    更新日期:1980-12-01 00:00:00

  • Structure and expression of the knob-associated histidine-rich protein of Plasmodium falciparum.

    abstract::cDNA clones encoding 473 amino acids of the knob-associated histidine-rich protein (PfHRPI) of Plasmodium falciparum clone FCR-3/A2 (Gambia) have been isolated and sequenced. Although a short region close to the amino terminus of the predicted sequence contains three blocks of six, seven or nine consecutive histidine ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ellis J,Irving DO,Wellems TE,Howard RJ,Cross GA

    更新日期:1987-11-01 00:00:00