Cloning and characterization of the ribosomal RNA gene repeat from Ostertagia ostertagi.

Abstract:

:Clones of the rRNA genes of Ostertagia ostertagi were selected from a library prepared from the genomic DNA of adult worms of strain LA-2. A 13.4-kb insert in a clone, lambda OOR78, is comprised of one complete 7.5-kb rDNA unit and portions of two adjacent units. The rDNA unit is directly repeated in a head-to-tail fashion and represents approximately 0.9% of the total genomic DNA. This repeating unit appears to be the only long tandemly repeated sequence in the genome. Restriction enzyme recognition sites in the rDNAs of four strains of O. ostertagi were fully conserved with the exception of one PstI site present in the large rRNA gene which was absent from a proportion of the genes of the LA-2 strain. The rDNA of O. ostertagi is more similar to that of Caenorhabditis elegans in unit length and arrangement than to parasitic helminths previously examined.

journal_name

Mol Biochem Parasitol

authors

Dame JB,Yowell CA,Courtney CH,Lindgren WG

doi

10.1016/0166-6851(91)90095-n

subject

Has Abstract

pub_date

1991-04-01 00:00:00

pages

275-80

issue

2

eissn

0166-6851

issn

1872-9428

pii

0166-6851(91)90095-N

journal_volume

45

pub_type

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