Proteolytic activity of Plasmodium falciparum subtilisin-like protease 3 on parasite profilin, a multifunctional protein.


:Subtilisin-like proteases of malaria parasite Plasmodium falciparum (PfSUB1, 2 and 3) are expressed at late asexual blood stages. PfSUB1 and 2 are considered important drug targets due to their essentiality for parasite blood stages and role in merozoite egress and invasion of erythrocytes. We have earlier shown the in vitro serine protease activity of PfSUB3 and its localization at asexual blood stages. In this study, we attempted to identify the biological substrate(s) of PfSUB3 and found parasite profilin (PfPRF) as a substrate of the protease. Eukaryotic profilins are multifunctional proteins with primary role in regulation of actin filament assembly. PfPRF possesses biochemical features of eukaryotic profilins and its rodent ortholog is essential in blood stages. Profilin from related apicomplexan parasite Toxoplasma gondii (TgPRF) is known to be involved in parasite motility, host cell invasion, active egress from host cell, immune evasion and virulence in mice. In this study, mature PfSUB3 proteolysed recombinant PfPRF in a dose-dependent manner in in vitro assays. Recombinant PfPRF was assessed for its proinflammatory activity and found to induce high level of TNF-α and low but significant level of IL-12 from mouse bone marrow-derived dendritic cells. Proteolysis of PfPRF by PfSUB3 is suggestive of the probable role of the protease in the processes of motility, virulence and immune evasion.


Mol Biochem Parasitol


Alam A,Bhatnagar RK,Relan U,Mukherjee P,Chauhan VS




Has Abstract


2013-10-01 00:00:00














  • Expression and cellular localization of Trypanosoma cruzi type II DNA topoisomerase.

    abstract::Topoisomerases are enzymes that participate in many cellular functions involving topological manipulation of DNA strands. There are two types of topoisomerases in the cell: (a) type I topoisomerases; and (b) type II topoisomerases (topo II). Previously we have cloned and sequenced the gene encoding Trypanosoma cruzi t...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Fragoso SP,Mattei D,Hines JC,Ray D,Goldenberg S

    更新日期:1998-08-01 00:00:00

  • Functional characterisation of a nematode secreted GM2-activator protein.

    abstract::We have identified a GM2-activator protein (GM2AP) with highly unusual properties secreted by the nematode parasite Trichinella spiralis. Expression in Pichia pastoris resulted in a hyperglycosylated protein of 28 kDa, but the 18 kDa native protein was not glycosylated. The parasite GM2AP does not facilitate degradati...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Bruce AF,Gares MP,Selkirk ME,Gounaris K

    更新日期:2006-06-01 00:00:00

  • Characterization of tubulin genes in Trypanosoma rangeli.

    abstract::Tubulin genes in Trypanosoma rangeli, the only trypanosome besides T. cruzi to infect humans in America, are organized in homogeneous, alternate alpha and beta gene tandem repeats of 3.8 kb. The basic repeat was cloned, mapped and partially sequenced. In contrast to most other eukaryotes, where tubulin genes are scatt...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Esquenazi D,Morel CM,Traub-Cseko YM

    更新日期:1989-05-15 00:00:00

  • Three genes and two isozymes: gene conversion and the compartmentalization and expression of the phosphoglycerate kinases of Trypanosoma (Nannomonas) congolense.

    abstract::The glycosome, a microbody organelle found only in kinetoplastid protozoa, compartmentalizes the first six enzymes of glycolysis. In order to better understand the regulation and targeting of glycolytic enzymes in trypanosomes, we have cloned and analyzed the three genes of the phosphoglycerate kinase (PGK) complex of...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Parker HL,Hill T,Alexander K,Murphy NB,Fish WR,Parsons M

    更新日期:1995-02-01 00:00:00

  • A unique Rab GTPase, EhRabA, of Entamoeba histolytica, localizes to the leading edge of motile cells.

    abstract::Entamoeba histolytica, an enteric protozoan parasite, infects 10% of the world's population leading to 50 million cases of invasive amoebiasis annually. Parasite vesicle trafficking and motility, which relies on vesicle trafficking to deliver membrane and membrane components to the leading edge, are important for viru...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Welter BH,Temesvari LA

    更新日期:2004-06-01 00:00:00

  • Structure and function of Per-ARNT-Sim domains and their possible role in the life-cycle biology of Trypanosoma cruzi.

    abstract::Per-ARNT-Sim (PAS) domains of proteins play important roles as modules for signalling and cellular regulation processes in widely diverse organisms such as Archaea, Bacteria, protists, plants, yeasts, insects and vertebrates. These domains are present in many proteins where they are used as sensors of stimuli and modu...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Rojas-Pirela M,Rigden DJ,Michels PA,Cáceres AJ,Concepción JL,Quiñones W

    更新日期:2018-01-01 00:00:00

  • Enzymes of purine and pyrimidine metabolism from the human malaria parasite, Plasmodium falciparum.

    abstract::Plasmodium falciparum trophozoites were isolated by mechanical rupture of infected human erythrocytes followed by a series of differential centrifugation steps. After lysis with sonication, the 100 000 x g supernatant of parasites and uninfected host cells was used to determine the specific activities of a number of e...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Reyes P,Rathod PK,Sanchez DJ,Mrema JE,Rieckmann KH,Heidrich HG

    更新日期:1982-05-01 00:00:00

  • Peptide-fluoromethyl ketones arrest intracellular replication and intercellular transmission of Trypanosoma cruzi.

    abstract::The major proteolytic activity of Trypanosoma cruzi is a cathepsin L-like cysteine protease expressed in all stages of the parasite. As an initial step in identifying possible functions of this enzyme in the life cycle of T. cruzi, and examining its potential as a target for rational drug design, two fluoromethyl keto...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Harth G,Andrews N,Mills AA,Engel JC,Smith R,McKerrow JH

    更新日期:1993-03-01 00:00:00

  • Isolation and functional characterization of snail hemocyte-modulating polypeptide from primary sporocysts of Schistosoma mansoni.

    abstract::Infection with larval trematodes has been shown to inhibit several snail-host defences, including hemocyte phagocytosis, cytotoxicity, motility, and adherence. Certain plasma factors which mediate snail defence responses, and which may be produced by host hemocytes, also appear to be altered by these parasites. In thi...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Lodes MJ,Connors VA,Yoshino TP

    更新日期:1991-11-01 00:00:00

  • In situ analysis of a variant surface glycoprotein expression-site promoter region in Trypanosoma brucei.

    abstract::In Trypanosoma brucei, the active variant surface glycoprotein genes (vsg) are located at telomeric expression sites (ES), whose expression is highly regulated during the life cycle. In the procyclic form, all ESs are repressed. In the bloodstream form, where antigenic variation occurs, only one of approximately 20 ES...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Navarro M,Cross GA

    更新日期:1998-07-01 00:00:00

  • High efficiency transfection of Plasmodium berghei facilitates novel selection procedures.

    abstract::The use of transfection in the study of the biology of malaria parasites has been limited due to poor transfection efficiencies (frequency of 10(-6) to 10(-9)) and a paucity of selection markers. Here, a new method of transfection, using non-viral Nucleofector technology, is described for the rodent parasite Plasmodiu...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Janse CJ,Franke-Fayard B,Mair GR,Ramesar J,Thiel C,Engelmann S,Matuschewski K,van Gemert GJ,Sauerwein RW,Waters AP

    更新日期:2006-01-01 00:00:00

  • Sialic acid-dependent binding of baculovirus-expressed recombinant antigens from Plasmodium falciparum EBA-175 to Glycophorin A.

    abstract::The Plasmodium falciparum Erythrocyte Binding Antigen-175, EBA-175, is a soluble merozoite stage parasite protein which binds to glycophorin A surface receptors on human erythrocytes. We have expressed two conserved cysteine-rich regions, region II and region VI, of this protein as soluble His-tagged polypeptides in i...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ockenhouse CF,Barbosa A,Blackall DP,Murphy CI,Kashala O,Dutta S,Lanar DE,Daugherty JR

    更新日期:2001-03-01 00:00:00

  • Cryptosporidium parvum cyclic GMP-dependent protein kinase (PKG): An essential mediator of merozoite egress.

    abstract::Cryptosporidiosis is an obligate intracellular pathogen causing diarrhea. Merozoite egress is essential for infection to spread between host cells. However, the mechanisms of egress have yet to be defined. We hypothesized that Cyclic GMP-Dependent Protein Kinase G (PKG) may be involved in Cryptosporidium egress. In th...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Nava S,Sadiqova A,Castellanos-Gonzalez A,White AC Jr

    更新日期:2020-05-01 00:00:00

  • Dominant lethality and insect population control.

    abstract::Recent advances in insect genetic engineering have opened up new possibilities in the genetic control of insect vectors of human diseases. We outline the current state of the sterile insect technique and show how the use of engineered dominant lethals can greatly increase the effectiveness of this approach. We conside...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章,评审


    authors: Alphey L,Andreasen M

    更新日期:2002-05-01 00:00:00

  • Homologous telomeric sequences are present in different species of the genus Plasmodium.

    abstract::The telomeric sequence cloned from Plasmodium berghei (see M. Ponzi et al. (1985) EMBO J. 4, 2991-2995) was tested for species specificity. A telomeric and a subtelomeric fragment of the cloned insert served as separate, labelled probes on pulsed field gradient electrophoretical patterns and on genomic digests from th...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Dore E,Pace T,Ponzi M,Scotti R,Frontali C

    更新日期:1986-11-01 00:00:00

  • Molecular basis of binding of the Plasmodium falciparum receptor BAEBL to erythrocyte receptor glycophorin C.

    abstract::Plasmodium falciparum invades human erythrocytes by redundant pathways. Unlike Plasmodium vivax that has one Duffy Binding-Like (DBL) receptor, P. falciparum has four members of the DBL receptor family. Furthermore, one of these DBL genes, BAEBL, has polymorphisms at four amino acids in region II; each polymorphism bi...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Jiang L,Duriseti S,Sun P,Miller LH

    更新日期:2009-11-01 00:00:00

  • The mature-parasite-infected erythrocyte surface antigen (MESA) of Plasmodium falciparum associates with the erythrocyte membrane skeletal protein, band 4.1.

    abstract::Several proteins synthesized by mature asexual stages of Plasmodium falciparum interact with the erythrocyte membrane skeleton. One of these is the mature-parasite-infected erythrocyte surface antigen (MESA; also called PfEMP2), a phosphoprotein of 250-300 kDa, which is found on the internal face of the erythrocyte me...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Lustigman S,Anders RF,Brown GV,Coppel RL

    更新日期:1990-01-15 00:00:00

  • Genomic organization, chromosomal location and transcription of dispersed and repeated tubulin genes in Leishmania major.

    abstract::The alpha and beta tubulin genes of Leishmania major were cloned and used to study the genomic organization, chromosomal location and transcription of tubulin genes in L. major. The number of beta tubulin isogenes was determined by hybridization of probes representing the 5' and 3' ends of the cloned beta tubulin cDNA...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Spithill TW,Samaras N

    更新日期:1987-05-01 00:00:00

  • Divergent evolutionary constraints on mitochondrial and nuclear genomes of malaria parasites.

    abstract::Genetic variation among malaria parasites has important consequences with regard to drug resistance, pathogenicity, immunity, transmission, and speciation. In this regard, malaria parasites have been shown to display a high degree of inter- and intra-species genetic divergence. The nuclear genomes of Plasmodium falcip...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: McIntosh MT,Srivastava R,Vaidya AB

    更新日期:1998-09-01 00:00:00

  • Trypanosoma brucei: the effect of glycerol on the anaerobic metabolism of glucose.

    abstract::Studies measuring the glycolytic intermediate and adenine nucleotide concentrations in Trypanosoma brucei metabolising glucose either aerobically or under conditions where glycerol-3-phosphate oxidase is inactive have shown the following: 1. Inhibition with 0.5 mM salicylhydroxamic acid (SHAM) accurately simulates ana...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Hammond DJ,Bowman IB

    更新日期:1980-12-01 00:00:00

  • Unusual diversity in alpha-amanitin sensitivity of RNA polymerases in trichomonads.

    abstract::Previous studies in the parasitic protist Trichomonas vaginalis have revealed that protein coding genes are transcribed by an alpha-amanitin-resistant RNA polymerase (RNAP) II. To investigate whether this unusual property is a general characteristic of trichomonads, we addressed the physiology of RNA synthesis in lyso...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Vanácová S,Tachezy J,Ullu E,Tschudi C

    更新日期:2001-07-01 00:00:00

  • Karyotype and synteny among the chromosomes of all four species of human malaria parasite.

    abstract::The karyotype and chromosomes of the human malaria parasite Plasmodium falciparum have been well characterized in recent years. Here we present karyotype maps of the three other human malaria species, P. vivax, P. malariae and P. ovale. Chromosomes of these species were found to be of significantly higher molecular we...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Carlton JM,Galinski MR,Barnwell JW,Dame JB

    更新日期:1999-06-25 00:00:00

  • Predicted structure of a major Schistosoma mansoni eggshell protein.

    abstract::The complete sequence for a major Schistosome mansoni eggshell protein gene has been determined from a genomic DNA fragment. The use of an open reading frame encoding a glycine-rich polypeptide was confirmed by in vitro translation of schistosome mRNA in the presence of [3H]glycine and comparison with the amino acid c...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Rodrigues V,Chaudhri M,Knight M,Meadows H,Chambers AE,Taylor WR,Kelly C,Simpson AJ

    更新日期:1989-01-01 00:00:00

  • RNA interference in Pratylenchus coffeae: knock down of Pc-pat-10 and Pc-unc-87 impedes migration.

    abstract::Many of the currently available nematicides used in nematode control are hazardous to the user, environment and beneficial non-target organisms. Therefore the need to develop alternative methods for nematode control such as the development of nematode-resistant crops through RNA-mediated interference (RNAi) holds grea...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Joseph S,Gheysen G,Subramaniam K

    更新日期:2012-11-01 00:00:00

  • The effects of p-chloromercuriphenylsulfonic acid on Trypanosoma cruzi infection of mammalian host cells in vitro.

    abstract::Treatment of Trypanosoma cruzi blood trypomastigotes with p-chloromercuriphenylsulfonic acid (PCMS) increased the association of the parasite with either mouse resident peritoneal macrophages or rat heart myoblasts in vitro. The effect was evidenced by elevation of both the percentage of host cells with parasites and ...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ayala A,Kierszenbaum F

    更新日期:1987-02-01 00:00:00

  • In vitro selection of halofantrine resistance in Plasmodium falciparum is not associated with increased expression of Pgh1.

    abstract::Recent investigations into quinoline and phenanthrene methanol resistance in Plasmodium falciparum have described a linkage between amplification of the mdr homologue pfmdr1 and decreased susceptibility to mefloquine (MQ) and halofantrine (HF). We have examined the current theories on cross-resistance patterns and pfm...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Ritchie GY,Mungthin M,Green JE,Bray PG,Hawley SR,Ward SA

    更新日期:1996-12-02 00:00:00

  • A novel multi-domain mucin-like glycoprotein of Cryptosporidium parvum mediates invasion.

    abstract::Cryptosporidium parvum is a protozoan parasite which produces self-limited disease in immunocompetent hosts and devastating, persistent diarrhea in immunocompromised individuals. There is no effective treatment for cryptosporidiosis and little is known about the basic biology of the organism. Cloning and sequence anal...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Barnes DA,Bonnin A,Huang JX,Gousset L,Wu J,Gut J,Doyle P,Dubremetz JF,Ward H,Petersen C

    更新日期:1998-10-30 00:00:00

  • Stable DNA transfection of a wide range of trypanosomatids.

    abstract::We have shown that the Leishmania major transfection vector pR-NEO (or derivatives thereof) can be introduced and stably maintained in four species complexes of pathogenic Leishmania (L. tropica, L. mexicana, L. donovani, L. braziliensis), and the genera Endotrypanum and Crithidia; transfection of Trypanosoma cruzi or...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Coburn CM,Otteman KM,McNeely T,Turco SJ,Beverley SM

    更新日期:1991-05-01 00:00:00

  • Antigenic diversity of the circumsporozoite proteins in the Plasmodium cynomolgi complex.

    abstract::Antigenic diversity was observed in the circumsporozoite (CS) proteins of five of the six Plasmodium cynomolgi isolates (NIH, Mulligan, London, Gombak, Ceylon, RO) that we examined. Monoclonal antibodies were produced against salivary gland sporozoites of three of the isolates. Interaction of these monoclonal antibodi...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Cochrane AH,Gwadz RW,Ojo-Amaize E,Hii J,Nussenzweig V,Nussenzweig RS

    更新日期:1985-01-01 00:00:00

  • Studies on mitochondrial ATPase of Leishmania donovani using digitonin-permeabilized promastigotes.

    abstract::Mitochondrial ATPase of Leishmania donovani was characterized using digitonin-permeabilized promastigotes and the results were compared with those from isolated mitochondria. Maximum mitochondrial ATPase activity was obtained in promastigotes permeabilized with digitonin at a final concentration of 20 microM and the s...

    journal_title:Molecular and biochemical parasitology

    pub_type: 杂志文章


    authors: Das A

    更新日期:1993-08-01 00:00:00