Abstract:
:The ability of Leishmania amastigotes to survive within the drastic environmental changes encountered in the phagolysosomes of mammalian macrophages is heavily dependent on the developmental regulation of a variety of genes. The identification of genes that are expressed preferentially in the mammalian stage of the parasite should increase our understanding of the molecular mechanisms regulating stage-specific gene expression and of the determinants that control its intracellular survival and contribute to its pathogenesis. We report here detailed sequence characterization and structural organization of the amastin gene family in Leishmania major and Leishmania infantum and the study of their developmental gene regulation throughout the parasite's life cycle. Amastin surface proteins represent the largest developmentally regulated gene family reported so far in Leishmania comprising up to 45 members. All the members of the amastin gene family in both Leishmania and Trypanosoma species share a similar structural organization and contain a highly conserved 11 amino acid extracellular domain, which is unique to amastin proteins. The majority of the amastin gene homologs are specifically expressed in the amastigote stage of the parasite. Three distinct RNA elements were identified in the 3'-untranslated regions (3'UTR) of the amastin transcripts. The majority of these transcripts contain a conserved 450 nt cis-acting 3'UTR element shown previously to regulate stage-specific gene expression at the level of translation, which suggests that several amastin homologs may be regulated by a similar mechanism of translational control inside the macrophage. These findings further highlight the unique features of gene expression control in Leishmania.
journal_name
Mol Biochem Parasitoljournal_title
Molecular and biochemical parasitologyauthors
Rochette A,McNicoll F,Girard J,Breton M,Leblanc E,Bergeron MG,Papadopoulou Bdoi
10.1016/j.molbiopara.2005.01.006subject
Has Abstractpub_date
2005-04-01 00:00:00pages
205-20issue
2eissn
0166-6851issn
1872-9428pii
S0166-6851(05)00024-1journal_volume
140pub_type
杂志文章abstract::Over the last years an expanding family of small RNAs (i.e. microRNAs, siRNAs and piRNAs) was recognized as key players in diverse forms of gene silencing and chromatin organization. Effectors functions of these small RNAs are achieved through ribonucleoprotein (RNP) complexes containing at their center an Argonaute/P...
journal_title:Molecular and biochemical parasitology
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journal_title:Molecular and biochemical parasitology
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journal_title:Molecular and biochemical parasitology
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pub_type: 杂志文章
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章
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更新日期:2007-02-01 00:00:00
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章
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更新日期:2004-04-01 00:00:00
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pub_type: 杂志文章
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journal_title:Molecular and biochemical parasitology
pub_type: 杂志文章
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